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Abdel-Rehim, MohamedORCID iD iconorcid.org/0000-0002-3862-8878
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Publications (10 of 14) Show all publications
Suzaei, F. M., Daryanavard, S. M., Abdel-Rehim, A., Bassyouni, F. & Abdel-Rehim, M. (2023). Recent molecularly imprinted polymers applications in bioanalysis. Chemické zvesti, 77(2), 619-655
Open this publication in new window or tab >>Recent molecularly imprinted polymers applications in bioanalysis
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2023 (English)In: Chemické zvesti, ISSN 0366-6352, E-ISSN 1336-9075, Vol. 77, no 2, p. 619-655Article in journal (Refereed) Published
Abstract [en]

Molecular imprinted polymers (MIPs) as extraordinary compounds with unique features have presented a wide range of applications and benefits to researchers. In particular when used as a sorbent in sample preparation methods for the analysis of biological samples and complex matrices. Its application in the extraction of medicinal species has attracted much attention and a growing interest. This review focus on articles and research that deals with the application of MIPs in the analysis of components such as biomarkers, drugs, hormones, blockers and inhibitors, especially in biological matrices. The studies based on MIP applications in bioanalysis and the deployment of MIPs in high-throughput settings and optimization of extraction methods are presented. A review of more than 200 articles and research works clearly shows that the superiority of MIP techniques lies in high accuracy, reproducibility, sensitivity, speed and cost effectiveness which make them suitable for clinical usage. Furthermore, this review present MIP-based extraction techniques and MIP-biosensors which are categorized on their classes based on common properties of target components. Extraction methods, studied sample matrices, target analytes, analytical techniques and their results for each study are described. Investigations indicate satisfactory results using MIP-based bioanalysis. According to the increasing number of studies on method development over the last decade, the use of MIPs in bioanalysis is growing and will further expand the scope of MIP applications for less studied samples and analytes.

Place, publisher, year, edition, pages
Springer Nature, 2023
Keywords
Bioanalysis, Biological samples, Extraction techniques, Molecular imprinted polymers
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-328107 (URN)10.1007/s11696-022-02488-3 (DOI)000862221100001 ()36213319 (PubMedID)2-s2.0-85139261097 (Scopus ID)
Note

QC 20230602

Available from: 2023-06-02 Created: 2023-06-02 Last updated: 2023-12-07Bibliographically approved
Suzaei, F. M., Batista, A. D., Mizaikoff, B., Rahimi, S., Daryanavard, S. M. & Abdel-Rehim, M. (2022). Molecularly imprinted polymers for selective extraction/microextraction of cancer biomarkers: A review. Microchimica Acta, 189(7), Article ID 255.
Open this publication in new window or tab >>Molecularly imprinted polymers for selective extraction/microextraction of cancer biomarkers: A review
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2022 (English)In: Microchimica Acta, ISSN 0026-3672, E-ISSN 1436-5073, Vol. 189, no 7, article id 255Article, review/survey (Refereed) Published
Abstract [en]

Over recent years, great efforts have been extensively documented in top scientific journals on the development of methods for early diagnosis, treatment, and monitoring of cancers which are prevalent critical diseases with a high mortality rate among men and women. The determination of cancer biomarkers using different optimum methodologies is one of the finest options for achieving these goals with more precision, speed, and at a lower cost than traditional clinical procedures. In this regard, while focusing on specific biomarkers, molecularly imprinted technology has enabled novel diagnostic techniques for a variety of diseases. Due to the well-known advantages of molecularly imprinted polymers (MIPs), this review focuses on the current trends of MIPs-based extraction/microextraction methods, specifically targeting cancer biomarkers from various matrices. These optimized methods have demonstrated high selectivity, accuracy, sorbent reusability, extraction recovery, and low limits of detection and quantification for a variety of cancer biomarkers, which are a powerful tool to provide early diagnosis, prognosis, and treatment monitoring, with potential clinical application expected soon. This review highlights the key progress, specific modifications, and strategies used for MIP synthesis. The future perspectives for cancer biomarkers purification and determination by fabricating MIP-based techniques are also discussed.

Place, publisher, year, edition, pages
Springer Nature, 2022
Keywords
Molecular Imprinted Polymers, Bioanalysis, Extraction, Microextraction, Cancer biomarkers, MIP, Separation
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-315249 (URN)10.1007/s00604-022-05356-9 (DOI)000810662100003 ()35697898 (PubMedID)2-s2.0-85131810831 (Scopus ID)
Note

QC 20220705

Available from: 2022-07-05 Created: 2022-07-05 Last updated: 2023-12-07Bibliographically approved
Chinnapaiyan, M., Selvam, Y., Bassyouni, F., Ramu, M., Sakkaraiveeranan, C., Samickannian, A., . . . Abdel-Rehim, M. (2022). Nanotechnology, Green Synthesis and Biological Activity Application of Zinc Oxide Nanoparticles Incorporated Argemone Mxicana Leaf Extract. Molecules, 27(5), Article ID 1545.
Open this publication in new window or tab >>Nanotechnology, Green Synthesis and Biological Activity Application of Zinc Oxide Nanoparticles Incorporated Argemone Mxicana Leaf Extract
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2022 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 27, no 5, article id 1545Article in journal (Refereed) Published
Abstract [en]

Nanomaterial is a rapidly growing area that is used to create a variety of new materials and nanotechnology applications from medical, pharmaceuticals, chemical, mechanical, electronics and several environmental industries including physical, chemical and biological nanoparticles are very important in our daily life. Nanoparticles with leaf extract from the healthy plant are important in the area of research using biosynthesis methods. Because of it's used as an environmentally ecofriendly, other than traditional physical and chemical strategies. In particular, biologically synthesized nanoparticles have become a key branch of nanotechnology. The present work presents a synthesis of zinc oxide nanoparticles using an extract from the Argemone leaf Mexicana. Biosynthetic nanoparticles are characterized by X-ray diffraction (XRD), Ultraviolet visible (UV-vis) spectroscopy analysis, a Fourier Transform Infrared Spectroscopy analysis (FTIR) and a scanning electron microcopy (SEM), X-ray analysis with dispersive energy (EDAX). XRD is used to examine the crystalline size of zinc oxide nanoparticles. The FTIR test consists in providing evidence of the presence of targeted teams. UV is used for optical properties and calculates the energy of the bandwidth slot. The scanning microscope emission reveals the morphology of the surface and the energy dispersive X-ray analysis confirms the basic composition of zinc oxide nanoparticles. It is found that zinc nanoparticles are capable of achieving high anti-fungal efficacy and therefore have a high potential antimicrobial activity of ZnO NPs, like antibacterial and high antioxidant. Zinc Oxide nanoparticles from the Argemone Mexicana leaf extract have several antimicrobial applications, such as medical specialty, cosmetics, food, biotechnology, nano medicine and drug delivery system. ZnO nanoparticles are important because they provide many practical applications in industry. The most important use of nanoparticles of ZnO would be strong antibacterial and antioxidant activity with a simple and efficient biosynthesis method may be used for future work applications.

Place, publisher, year, edition, pages
MDPI AG, 2022
Keywords
nanoparticles ZnO NPs, green biosynthesis, characterization techniques, antibacterial and antifungal activities, antioxidant activity
National Category
Materials Chemistry
Identifiers
urn:nbn:se:kth:diva-310214 (URN)10.3390/molecules27051545 (DOI)000768210800001 ()35268646 (PubMedID)2-s2.0-85125194334 (Scopus ID)
Note

QC 20220325

Available from: 2022-03-25 Created: 2022-03-25 Last updated: 2023-12-07Bibliographically approved
Zohdijamil, Z., Hashemi, M., Abdel-Rehim, A., Laxman, K., Uheida, A., Dutta, J. & Abdel-Rehim, M. (2021). Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids: Extraction of ritonavir, a HIV protease inhibitor, from human saliva and plasma using LC–MS/MS. BMC Biomedical chromotography, 35(12), Article ID e5111.
Open this publication in new window or tab >>Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids: Extraction of ritonavir, a HIV protease inhibitor, from human saliva and plasma using LC–MS/MS
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2021 (English)In: BMC Biomedical chromotography, ISSN 0269-3879, E-ISSN 1099-0801, Vol. 35, no 12, article id e5111Article in journal (Refereed) Published
Abstract [en]

In this work, graphene oxide–based tablets (GO-Tabs) were prepared by applying a thin layer of functionalized GO on a polyethylene substrate. The GO was functionalized with amine groups (–NH2) by poly(ethylene glycol)bis(3-aminopropyl) terminated (GO-NH2-PEG-NH2). The functionalized GO-Tabs were used for the extraction of ritonavir (RTV) in human saliva samples. RTV in plasma and saliva samples was analyzed using LC–MS/MS. Gradient LC system with MS/MS in the positive-ion mode [electrospray ionization (ESI+)] was used. The transitions m/z 721 → 269.0 and m/z 614 → 421 were used for RTV and the internal standard indinavir, respectively. This study determined the human immunodeficiency virus protease inhibitor RTV in human saliva samples using functionalized GO-Tab and LC–MS/MS, and the method was validated. The standard calibration curve for plasma and saliva samples was constructed from 5.0 to 2000 nmol L−1. The limit of detection was 0.1 nmol L−1, and the limit of quantification was 5.0 nmol L−1 in both plasma and saliva matrices. The intra- and inter-assay precision values were found to be between 1.5 and 5.8%, and the accuracy values ranged from 88.0 to 108% utilizing saliva and plasma samples. The extraction recovery was more than 80%, and the presented functionalized GO-Tabs could be reused for more than 10 extractions without deterioration in recovery.

Place, publisher, year, edition, pages
Wiley, 2021
Keywords
graphene oxide tablets, LC–MS/MS, plasma, ritonavir, saliva, amine, graphene oxide, macrogol derivative, nanocomposite, poly(ethylene glycol)bis (3 aminopropyl), polyethylene, unclassified drug, graphite, Human immunodeficiency virus proteinase inhibitor, nanomaterial, blood sampling, Conference Paper, drug determination, drug synthesis, human, hydrogen bond, limit of detection, limit of quantitation, liquid chromatography-mass spectrometry, positive ion electrospray, saliva analysis, tablet, chemistry, liquid chromatography, procedures, reproducibility, statistical model, tablet manufacture, tandem mass spectrometry, Chromatography, Liquid, HIV Protease Inhibitors, Humans, Linear Models, Nanostructures, Reproducibility of Results, Tablets
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-309668 (URN)10.1002/bmc.5111 (DOI)000656746500001 ()33675066 (PubMedID)2-s2.0-85107333551 (Scopus ID)
Note

QC 20220314

Available from: 2022-03-14 Created: 2022-03-14 Last updated: 2024-03-18Bibliographically approved
Bassyouni, F., Tarek, M., Salama, A., Ibrahim, B., Salah El Dine, S., Yassin, N., . . . Abdel-Rehim, M. (2021). Promising Antidiabetic and Antimicrobial Agents Based on Fused Pyrimidine Derivatives: Molecular Modeling and Biological Evaluation with Histopathological Effect. Molecules, 26(8), Article ID 2370.
Open this publication in new window or tab >>Promising Antidiabetic and Antimicrobial Agents Based on Fused Pyrimidine Derivatives: Molecular Modeling and Biological Evaluation with Histopathological Effect
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2021 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 26, no 8, article id 2370Article in journal (Refereed) Published
Abstract [en]

Diabetes is the most common metabolic disorder in both developing and non-developing countries, and a well-recognized global health problem. The WHO anticipates an increase in cases from 171 million in 2000 to 366 million by 2030. In the present study, we focus on the preparation of pyrimidine derivatives as potential antidiabetic and antimicrobial agents. Thein vivoeffect on total serum glucose concentration, cholesterol and antioxidant activity was assessed in adult male albino Wister rats and compared to the reference drug glimperide. Promising results were observed for compound 5. The histopathological study confirms that compound 5 results in significant activity with liver maintenance. The antimicrobial activities were evaluated against several bacterial strains such as Salmonella typhimurium ATCC 25566, Bacillus cereus, Escherichia coli NRRN 3008, Pseudomonas aeruginosa ATCC 10145, Staphylococcus aureus ATCC 6538and fungi such as Rhizopus oligosporus, Mucor miehei and Asperillus niger. Compounds 4 and 5 showed a good inhibition of the bacterial zone compared to the reference drug cephradine. Finally, we suggest protein targets for these drugs based on computational analysis, and infer their activities from their predicted modes of binding using molecular modeling. The molecular modeling for compounds 4 and 5 resulted in improved docking scores and hydrogen bonding. The docking studies are in good agreement with the in vitro and in vivo studies.

Place, publisher, year, edition, pages
MDPI AG, 2021
Keywords
molecular modeling, pyrimidine derivatives, antidiabetic activity, antimicrobial activity, histopathological study
National Category
Medicinal Chemistry
Identifiers
urn:nbn:se:kth:diva-295370 (URN)10.3390/molecules26082370 (DOI)000644618100001 ()33921827 (PubMedID)2-s2.0-85105241106 (Scopus ID)
Note

QC 20210524

Available from: 2021-05-24 Created: 2021-05-24 Last updated: 2023-12-07Bibliographically approved
Daryanavard, S. M., Zolfaghari, H., Abdel-Rehim, A. & Abdel-Rehim, M. (2021). Recent applications of microextraction sample preparation techniques in biological samples analysis. BMC Biomedical chromotography, 35(7), Article ID e5105.
Open this publication in new window or tab >>Recent applications of microextraction sample preparation techniques in biological samples analysis
2021 (English)In: BMC Biomedical chromotography, ISSN 0269-3879, E-ISSN 1099-0801, Vol. 35, no 7, article id e5105Article in journal (Refereed) Published
Abstract [en]

Analysis of biological samples is affected by interfering substances with chemical properties similar to those of the target analytes, such as drugs. Biological samples such as whole blood, plasma, serum, urine and saliva must be properly processed for separation, purification, enrichment and chemical modification to meet the requirements of the analytical instruments. This causes the sample preparation stage to be of undeniable importance in the analysis of such samples through methods such as microextraction techniques. The scope of this review will cover a comprehensive summary of available literature data on microextraction techniques playing a key role for analytical purposes, methods of their implementation in common biological samples, and finally, the most recent examples of application of microextraction techniques in preconcentration of analytes from urine, blood and saliva samples. The objectives and merits of each microextration technique are carefully described in detail with respect to the nature of the biological samples. This review presents the most recent and innovative work published on microextraction application in common biological samples, mostly focused on original studies reported from 2017 to date. The main sections of this review comprise an introduction to the microextraction techniques supported by recent application studies involving quantitative and qualitative results and summaries of the most significant, recently published applications of microextracion methods in biological samples. This article considers recent applications of several microextraction techniques in the field of sample preparation for biological samples including urine, blood and saliva, with consideration for extraction techniques, sample preparation and instrumental detection systems.

Place, publisher, year, edition, pages
Wiley, 2021
Keywords
biological samples, microextraction techniques, sample preparation, sorbent, biological marker, analytic method, blood analysis, blood purification, blood sampling, body fluid, capillary electrophoresis, chemical analysis, chemical modification, concentration process, dispersive liquid liquid microextraction, electromembrane extraction, gas chromatography, human, liquid chromatography, liquid phase microextraction, microextraction by packed sorbent, microextraction sample preparation technique, phase separation, plasma, qualitative analysis, quantitative analysis, Review, saliva analysis, serum, single drop microextraction, solid phase extraction, solid phase microextraction, solvent bar microextraction, stir bar sorptive extraction, thin film microextraction, urinalysis, urine sampling, analysis, animal, rat, specimen handling, Analytic Sample Preparation Methods, Animals, Biomarkers, Humans, Rats
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-309671 (URN)10.1002/bmc.5105 (DOI)000643139100001 ()33660303 (PubMedID)2-s2.0-85107134809 (Scopus ID)
Note

QC 20220316

Available from: 2022-03-16 Created: 2022-03-16 Last updated: 2023-12-07Bibliographically approved
Uheida, A., Mejía, H. G., Abdel-Rehim, M., Hamd, W. & Dutta, J. (2021). Visible light photocatalytic degradation of polypropylene microplastics in a continuous water flow system. Journal of Hazardous Materials, 406, Article ID 124299.
Open this publication in new window or tab >>Visible light photocatalytic degradation of polypropylene microplastics in a continuous water flow system
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2021 (English)In: Journal of Hazardous Materials, ISSN 0304-3894, E-ISSN 1873-3336, Vol. 406, article id 124299Article in journal (Refereed) Published
Abstract [en]

Microplastic pollution of water and ecosystem is attracting continued attention worldwide. Due to their small sizes (≤5 mm) microplastic particles can be discharged to the environment from treated wastewater effluents. As microplastics have polluted most of our aquatic ecosystems, often finding its way into drinking water, there is urgent need to find new solutions for tackling the menace of microplastic pollution. In this work, sustainable green photocatalytic removal of microplastics from water activated by visible light is proposed as a tool for the removal of microplastics from water. We propose a novel strategy for the elimination of microplastics using glass fiber substrates to trap low density microplastic particles such as polypropylene (PP) which in parallel support the photocatalyst material. Photocatalytic degradation of PP microplastics spherical particles suspended in water by visible light irradiation of zinc oxide nanorods (ZnO NRs) immobilized onto glass fibers substrates in a flow through system is demonstrated. Upon irradiation of PP microplastics for two weeks under visible light reduced led to a reduction of the average particle volume by 65%. The major photodegradation by-products were identified using GC/MS and found to be molecules that are considered to be mostly nontoxic in the literature.

Place, publisher, year, edition, pages
Elsevier B.V., 2021
Keywords
Microplastics, Microplastics removal, Photocatalysis, Polypropylene particles, ZnO NRs, Aquatic ecosystems, Effluents, Flow of water, Glass fibers, II-VI semiconductors, Irradiation, Light, Nanorods, Oxide minerals, Photocatalytic activity, Photodegradation, Polypropylenes, Potable water, Wastewater treatment, Water pollution, Zinc oxide, Flow-through systems, Microplastic particles, Photo catalytic degradation, Photo-catalytic removal, Photocatalyst materials, Treated wastewater, Visible-light irradiation, Zinc oxide nanorods, Microplastic, byproduct, discharge, drinking water, glass, immobilization, oxide group, photochemistry, plastic, visible spectrum
National Category
Environmental Sciences
Identifiers
urn:nbn:se:kth:diva-290248 (URN)10.1016/j.jhazmat.2020.124299 (DOI)000662119400005 ()33131945 (PubMedID)2-s2.0-85094808761 (Scopus ID)
Note

QC 20210720

Available from: 2021-03-19 Created: 2021-03-19 Last updated: 2023-12-07Bibliographically approved
Abdel-Rehim, M., Pedersen-Bjergaard, S., Abdel-Rehim, A., Lucena, R., Moein, M. M., Cárdenas, S. & Miró, M. (2020). Microextraction approaches for bioanalytical applications: An overview. Journal of Chromatography A, 1616, Article ID 460790.
Open this publication in new window or tab >>Microextraction approaches for bioanalytical applications: An overview
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2020 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1616, article id 460790Article in journal (Refereed) Published
Abstract [en]

Biological samples are usually complex matrices due to the presence of proteins, salts and a variety of organic compounds with chemical properties similar to those of the target analytes. Therefore, sample preparation is often mandatory in order to isolate the analytes from troublesome matrices before instrumental analysis. Because the number of samples in drug development, doping analysis, forensic science, toxicological analysis, and preclinical and clinical assays is steadily increasing, novel high throughput sample preparation approaches are calling for. The key factors in this development are the miniaturization and the automation of the sample preparation approaches so as to cope with most of the twelve principles of green chemistry. In this review, recent trends in sample preparation and novel strategies will be discussed in detail with particular focus on sorptive and liquid-phase microextraction in bioanalysis. The actual applicability of selective sorbents is also considered. Additionally, the role of 3D printing in microextraction for bioanalytical methods will be pinpointed.

Place, publisher, year, edition, pages
Elsevier B.V., 2020
Keywords
3D printing, Bioanalysis, Microextraction approaches, Nanomaterials, Sample preparation, 3D printers, Chemical analysis, Nanostructured materials, 3-D printing, Bioanalytical applications, Bioanalytical methods, Liquid phase micro extraction, Microextraction, Toxicological analysis, Extraction
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-272249 (URN)10.1016/j.chroma.2019.460790 (DOI)000527313200015 ()31892411 (PubMedID)2-s2.0-85076993211 (Scopus ID)
Note

QC 20200422

Available from: 2020-04-22 Created: 2020-04-22 Last updated: 2023-12-07Bibliographically approved
Zohdi, Z., Hashemi, M., Uheida, A., Moein, M. M. & Abdel-Rehim, M. (2019). Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry. Molecules, 24(7), Article ID 1191.
Open this publication in new window or tab >>Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry
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2019 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 24, no 7, article id 1191Article in journal (Refereed) Published
Abstract [en]

In this study, a novel sort of sample preparation sorbent was developed, by preparing thin layer graphene oxide tablets (GO-Tabs) utilizing a mixture of graphene oxide and polyethylene glycol on a polyethylene substrate. The GO-Tabs were used for extraction and concentration of omeprazole (OME) in human saliva samples. The determination of OME was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) under gradient LC conditions and in the positive ion mode (ESI+) with mass transitions of m/z 346.3 -> 198.0 for OME and m/z 369.98 -> 252.0 for the internal standard. Standard calibration for the saliva samples was in the range of 2.0-2000 nmol L-1. Limits of detection and quantification were 0.05 and 2.0 nmol L-1, respectively. Method validation showed good method accuracy and precision; the inter-day precision values ranged from 5.7 to 8.3 (%RSD), and the accuracy of determinations varied from -11.8% to 13.3% (% deviation from nominal values). The extraction recovery was 60%, and GO-Tabs could be re-used for more than ten extractions without deterioration in recovery. In this study, the determination of OME in real human saliva samples using GO-Tab extraction was validated.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
graphene oxide, omeprazole, liquid chromatography, tandem mass spectrometry, saliva, GO-Tabs
National Category
Biophysics
Identifiers
urn:nbn:se:kth:diva-251343 (URN)10.3390/molecules24071191 (DOI)000464946300002 ()30934659 (PubMedID)2-s2.0-85063759590 (Scopus ID)
Note

QC 20190521

Available from: 2019-05-21 Created: 2019-05-21 Last updated: 2023-12-07Bibliographically approved
Karimiyan, H., Hadjmohammadi, M. R., Laxman, K., Moein, M. M., Dutta, J. & Abdel-Rehim, M. (2019). Graphene Oxide/Polyethylene Glycol-Stick for Thin Film Microextraction of beta-Blockers from Human Oral Fluid by Liquid Chromatography-Tandem Mass Spectrometry. Molecules, 24(20)
Open this publication in new window or tab >>Graphene Oxide/Polyethylene Glycol-Stick for Thin Film Microextraction of beta-Blockers from Human Oral Fluid by Liquid Chromatography-Tandem Mass Spectrometry
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2019 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 24, no 20Article in journal (Refereed) Published
Abstract [en]

A wooden stick coated with a novel graphene-based nanocomposite (Graphene oxide/polyethylene glycol (GO/PEG)) is introduced and investigated for its efficacy in solid phase microextraction techniques. The GO/PEG-stick was prepared and subsequently applied for the extraction of beta -blockers, acebutolol, and metoprolol in human oral fluid samples, which were subsequently detected by liquid chromatography tandem mass spectrometry (LC-MS/MS). Experimental parameters affecting the extraction protocol including sample pH, extraction time, desorption time, appropriate desorption solvent, and salt addition were optimized. Method validation for the detection from oral fluid samples was performed following FDA (Food and Drug Administration) guidelines on bioanalytical method validation. Calibration curves ranging from 5.0 to 2000 nmol L-1 for acebutolol and 25.0 to 2000 nmol L-1 for metoprolol were used. The values for the coefficient of determination (R-2) were found to be 0.998 and 0.996 (n = 3) for acebutolol and metoprolol, respectively. The recovery of analytes during extraction was 80.0% for acebutolol and 62.0% for metoprolol, respectively. The limit of detections (LODs) were 1.25, 8.00 nmol L-1 for acebutolol and metoprolol and the lower limit of quantifications (LLOQ) were 5.00 nmol L-1 for acebutolol and 25.0 nmol L-1 for metoprolol. Validation experiments conducted with quality control (QC) samples demonstrated method accuracy between 80.0% to 97.0% for acebutolol and from 95.0% to 109.0% for metoprolol. The inter-day precision for QC samples ranged from 3.6% to 12.9% for acebutolol and 9.5% to 11.3% for metoprolol. Additionally, the GO/PEG-stick was demonstrated to be reusable, with the same stick observed to be viable for more than 10 extractions from oral fluid samples.

Place, publisher, year, edition, pages
MDPI, 2019
Keywords
graphene oxide, polyethylene glycol, wooden stick, beta-blocker, oral fluid, LC-MS, MS
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-264869 (URN)10.3390/molecules24203664 (DOI)000496249500033 ()31614604 (PubMedID)2-s2.0-85073427233 (Scopus ID)
Note

QC 20191217

Available from: 2019-12-17 Created: 2019-12-17 Last updated: 2023-12-07Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-3862-8878

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