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van der Wijngaart, WouterORCID iD iconorcid.org/0000-0001-8248-6670
Alternative names
Publications (10 of 176) Show all publications
Guo, M., Madaboosi, N., Neumann, F., Nilsson, M. & van der Wijngaart, W. (2019). Direct Electrical Detection of sub-aM DNA concentrations.
Open this publication in new window or tab >>Direct Electrical Detection of sub-aM DNA concentrations
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2019 (English)Article in journal (Other academic) Submitted
National Category
Electrical Engineering, Electronic Engineering, Information Engineering Medical and Health Sciences Nano Technology
Identifiers
urn:nbn:se:kth:diva-250773 (URN)
Note

QC 20190506

Available from: 2019-05-06 Created: 2019-05-06 Last updated: 2019-05-14Bibliographically approved
Zandi Shafagh, R., Decrop, D., Ven, K., Vanderbeke, A., Hanusa, R., Breukers, J., . . . van der Wijngaart, W. (2019). Reaction Injection Molding of Hydrophilic-in-Hydrophobic Femtolitre-Well Arrays. Microsystems & Nanoengineering (5), Article ID 25.
Open this publication in new window or tab >>Reaction Injection Molding of Hydrophilic-in-Hydrophobic Femtolitre-Well Arrays
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2019 (English)In: Microsystems & Nanoengineering, E-ISSN 2055-7434, no 5, article id 25Article in journal (Refereed) Published
Abstract [en]

Patterning of micro- and nanoscale topologies and surface properties of polymer devices is of particular importance for a broad range of life science applications, including cell-adhesion assays and highly sensitive bioassays. The manufacturing of such devices necessitates cumbersome multiple-step fabrication procedures and results in surface properties which degrade over time. This critically hinders their wide-spread dissemination. Here, we simultaneously mold and surface energy pattern microstructures in off-stoichiometric thiol-ene by area-selective monomer self-assembly in a rapid micro-reaction injection molding cycle. We replicated arrays of 1,843,650 hydrophilic-in-hydrophobic femtolitre-wells with long-term stable surface properties and magnetically trapped beads with 75% and 87.2% efficiency in single- and multiple-seeding events, respectively. These results form the basis for ultrasensitive digital biosensors, specifically, and for the fabrication of medical devices and life science research tools, generally.

Place, publisher, year, edition, pages
Nature Publishing Group, 2019
Keywords
Reaction injection molding
National Category
Nano Technology
Identifiers
urn:nbn:se:kth:diva-248300 (URN)10.1038/s41378-019-0065-2 (DOI)000470930600001 ()
Note

QC 20190405

Available from: 2019-04-04 Created: 2019-04-04 Last updated: 2019-10-28Bibliographically approved
Guo, W., Hansson, J. & van der Wijngaart, W. (2019). SYNTHETIC MICROFLUIDIC PAPER WITH SUPERIOR FLUORESCENT SIGNAL READOUT. In: Proceedings of The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences: . Paper presented at The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019), 27-31 October 2019, Basel, SWITZERLAND (pp. 1056-1057).
Open this publication in new window or tab >>SYNTHETIC MICROFLUIDIC PAPER WITH SUPERIOR FLUORESCENT SIGNAL READOUT
2019 (English)In: Proceedings of The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2019, p. 1056-1057Conference paper, Published paper (Refereed)
Abstract [en]

This work is the first report on the use of synthetic microfluidic paper for lateral flow immunoassays. We grafted test lines of biotin on the synthetic paper using the thiol-yne “click” reaction. We captured fluorescently labeled streptavidin in a lateral flow fashion. Our two main findings are that, compared to other polymer lateral flow substrates with similar surface area, the synthetic microfluidic paper geometry results in 1) a stronger and more stable fluorescent signal per capture area, and 2) a sensitivity ~7 times higher.

Keywords
synthetic paper, fluorescent signal, biotin-steptavidin, OSTE, capillary, lateral flow test, off-stoichiometery thiol-ene, thiol-yne
National Category
Electrical Engineering, Electronic Engineering, Information Engineering Paper, Pulp and Fiber Technology Polymer Technologies
Research subject
Biotechnology; Electrical Engineering; Chemistry
Identifiers
urn:nbn:se:kth:diva-263249 (URN)978-1-7334190-0-0 (ISBN)
Conference
The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2019), 27-31 October 2019, Basel, SWITZERLAND
Projects
ND4ID
Note

QC 20191106

Available from: 2019-11-05 Created: 2019-11-05 Last updated: 2019-11-06Bibliographically approved
Imani Jajarmi, R., Ladhani, L., Pardon, G., van der Wijngaart, W. & Robert, E. (2019). The Influence of Air Flow Velocity and Particle Size on the Collection Efficiency of Passive Electrostatic Aerosol Samplers. Aerosol and Air Quality Research, 19(2), 192-203
Open this publication in new window or tab >>The Influence of Air Flow Velocity and Particle Size on the Collection Efficiency of Passive Electrostatic Aerosol Samplers
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2019 (English)In: Aerosol and Air Quality Research, ISSN 1680-8584, E-ISSN 2071-1409, Vol. 19, no 2, p. 192-203Article in journal (Refereed) Published
Abstract [en]

Electrostatic sampling is a promising method for the collection of bioaerosol particles. Although the underlying physics responsible for particle collection are well understood, the collection efficiency of simple passive electrostatic samplers is difficult to predict. Under these conditions, the collection efficiency becomes very sensitive to ambient air current and particle size, especially for submicron particles relevant for airborne virus transmission. In this paper, we compare two electrostatic aerosol sampler designs, a commercial product consisting of a flat collector plate located in the same plane as the charging needles and an axisymmetric design sampling directly to a liquid droplet. The aerosol particle collection efficiency of the samplers is investigated for particle size ranging from 0.25 to 2 µm while the air flow velocity surrounding the samplers is varied from 0.3 to 1 m s–1. For the planar design, at all ambient flow velocities, the submicron fraction of the particles captured originates in streamlines up to a maximum of 75 mm above the surface of the device collector, which greatly limits the volume of air being effectively sampled. The axisymmetric design features a non-monotonic capture efficiency as a function of particle size, with a minimum between 0.4 and 0.8 µm. The flow field in the inter-electrode region, captured using particle image velocimetry (PIV) reveals the presence of strong recirculation zones that can be responsible for the increased collection efficiency for very small particles.

National Category
Other Engineering and Technologies
Identifiers
urn:nbn:se:kth:diva-243837 (URN)10.4209/aaqr.2018.06.0211 (DOI)000457195200001 ()2-s2.0-85063957081 (Scopus ID)
Projects
NOROSENSOR
Note

QC 20190213

Available from: 2019-02-06 Created: 2019-02-06 Last updated: 2019-05-16Bibliographically approved
da Silva Granja, C., Sandström, N., Efimov, I., Ostanin, V., van der Wijngaart, W., Klenerman, D. & Ghosh, S. (2018). Characterisation of particle-surface interactions via anharmonic acoustic transduction. Sensors and actuators. B, Chemical, 272, 175-184
Open this publication in new window or tab >>Characterisation of particle-surface interactions via anharmonic acoustic transduction
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2018 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 272, p. 175-184Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Elsevier, 2018
National Category
Nano Technology
Identifiers
urn:nbn:se:kth:diva-235182 (URN)10.1016/j.snb.2018.05.016 (DOI)000439715000023 ()2-s2.0-85048499666 (Scopus ID)
Projects
Norosensor
Note

QC 20181003

Available from: 2018-09-17 Created: 2018-09-17 Last updated: 2019-04-12Bibliographically approved
Iseri, E., Akay, S. & van der Wijngaart, W. (2018). Detection of E.coli in a digital assay. In: 2018 IEEE Micro Electro Mechanical Systems (MEMS): . Paper presented at 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21 January 2018 through 25 January 2018 (pp. 301-303). Institute of Electrical and Electronics Engineers (IEEE)
Open this publication in new window or tab >>Detection of E.coli in a digital assay
2018 (English)In: 2018 IEEE Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers (IEEE), 2018, p. 301-303Conference paper, Published paper (Refereed)
Abstract [en]

In this paper, we demonstrate the dipstick-based digitisation and detection of bacterial sample of concentration down to 103 CFU/ml. The significance of this work is that we are able to detect concentrations of bacteria relevant for urinary tract infection (UTI) with minimal handling time and without the need for complicated external equipment.

Place, publisher, year, edition, pages
Institute of Electrical and Electronics Engineers (IEEE), 2018
Series
Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), ISSN 1084-6999
National Category
Microbiology in the medical area
Identifiers
urn:nbn:se:kth:diva-228551 (URN)10.1109/MEMSYS.2018.8346545 (DOI)000434960900080 ()2-s2.0-85047007412 (Scopus ID)9781538647820 (ISBN)
Conference
31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21 January 2018 through 25 January 2018
Funder
EU, Horizon 2020, 675412
Note

QC 20180528

Available from: 2018-05-28 Created: 2018-05-28 Last updated: 2018-09-05Bibliographically approved
Yasuga, H., Guo, W., Hansson, J., Haraldsson, T., Miki, N. & van der Wijngaart, W. (2018). Droplet microfluidics inside paper. In: 2018 IEEE Micro Electro Mechanical Systems (MEMS): . Paper presented at 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21 January 2018 through 25 January 2018 (pp. 269-271). Institute of Electrical and Electronics Engineers (IEEE)
Open this publication in new window or tab >>Droplet microfluidics inside paper
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2018 (English)In: 2018 IEEE Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers (IEEE), 2018, p. 269-271Conference paper, Published paper (Refereed)
Abstract [en]

Here, we demonstrate, for the first time: the self-digitization, i.e. spontaneous formation, of microdroplets during the imbibition of paper; the on-demand merging of individual microdroplets in paper; and the on-demand ejection of individual microdroplets from the paper. Two technical novelties underlie these novel functions: the formation of free-standing synthetic microfluidic paper, i.e. a porous matrix of slanted and interconnected micropillars without bottom layer; and the hydrophobic surface modification of the paper. The ease of manipulation and the direct access to the microdroplets from the environment makes this an extremely versatile tool, with potential applications in liquid sample digitisation and microparticle generation.

Place, publisher, year, edition, pages
Institute of Electrical and Electronics Engineers (IEEE), 2018
National Category
Other Engineering and Technologies not elsewhere specified
Identifiers
urn:nbn:se:kth:diva-228552 (URN)10.1109/MEMSYS.2018.8346536 (DOI)000434960900071 ()2-s2.0-85047016277 (Scopus ID)9781538647820 (ISBN)
Conference
31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21 January 2018 through 25 January 2018
Note

QC 20180528

Available from: 2018-05-28 Created: 2018-05-28 Last updated: 2018-09-05Bibliographically approved
Shafagh, R. Z., Vastesson, A., Guo, W., van der Wijngaart, W. & Haraldsson, K. T. (2018). E-Beam Nanostructuring and Direct Click Biofunctionalization of Thiol–Ene Resist. ACS Nano, 12(10), 9940-9946
Open this publication in new window or tab >>E-Beam Nanostructuring and Direct Click Biofunctionalization of Thiol–Ene Resist
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2018 (English)In: ACS Nano, ISSN 1936-0851, E-ISSN 1936-086X, Vol. 12, no 10, p. 9940-9946Article in journal (Refereed) Published
Abstract [en]

Electron beam lithography (EBL) is of major importance for ultraminiaturized biohybrid system fabrication, as it allows combining biomolecular patterning and mechanical structure definition on the nanoscale. Existing methods are limited by multistep biomolecule immobilization procedures, harsh processing conditions that are harmful to sensitive biomolecules, or the structural properties of the resulting protein monolayers or hydrogel-based resists. This work introduces a thiol-ene EBL resist with chemically reactive thiol groups on its native surface that allow the direct and selective "click" immobilization of biomolecules under benign processing conditions. We constructed EBL structured features of size down to 20 nm, and direct functionalized the nanostructures with a sandwich of biotin and streptavidin. The facile combination of polymer nanostructuring with biomolecule immobilization enables mechanically robust biohybrid components of interest for nanoscale biomedical, electronic, photonic, and robotic applications.

Keywords
OSTE; biohybrid; e-beam; nanoscale; NEMS; protein patterning; resist; thiol−ene
National Category
Nano Technology
Identifiers
urn:nbn:se:kth:diva-236089 (URN)10.1021/acsnano.8b03709 (DOI)000448751800030 ()2-s2.0-8505371109 (Scopus ID)
Note

QC 20181114

Available from: 2018-10-16 Created: 2018-10-16 Last updated: 2019-04-29Bibliographically approved
Guo, M., Hernández-Neuta, I., Madaboosi, N., Nilsson, M. & van der Wijngaart, W. (2018). Efficient DNA-assisted synthesis of trans-membrane gold nanowires. Microsystems & Nanoengineering, 4, 1-8, Article ID UNSP 17084.
Open this publication in new window or tab >>Efficient DNA-assisted synthesis of trans-membrane gold nanowires
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2018 (English)In: Microsystems & Nanoengineering, ISSN 2055-7434, Vol. 4, p. 1-8, article id UNSP 17084Article in journal (Refereed) Published
Abstract [en]

Whereas electric circuits and surface-based (bio)chemical sensors are mostly constructed in-plane due to ease of manufacturing, 3D microscale and nanoscale structures allow denser integration of electronic components and improved mass transport of the analyte to (bio)chemical sensor surfaces. This work reports the first out-of-plane metallic nanowire formation based on stretching of DNA through a porous membrane. We use rolling circle amplification (RCA) to generate long single-stranded DNA concatemers with one end anchored to the surface. The DNA strands are stretched through the pores in the membrane during liquid removal by forced convection. Because the liquid–air interface movement across the membrane occurs in every pore, DNA stretching across the membrane is highly efficient. The stretched DNA molecules are transformed into trans-membrane gold nanowires through gold nanoparticle hybridization and gold enhancement chemistry. A 50 fM oligonucleotide concentration, a value two orders of magnitude lower than previously reported for flat surface-based nanowire formation, was sufficient for nanowire formation. We observed nanowires in up to 2.7% of the membrane pores, leading to an across-membrane electrical conductivity reduction from open circuit to o20 Ω. The simple electrical read-out offers a high signal-to-noise ratio and can also be extended for use as a biosensor due to the high specificity and scope for multiplexing offered by RCA.

National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:kth:diva-227196 (URN)10.1038/micronano.2017.84 (DOI)000425451300001 ()
Funder
Swedish Research Council, SBE13-0125Swedish Foundation for Strategic Research , SBE13-0125EU, Horizon 2020, 675412
Note

Correction in: Microsystems & Nanoengineering (2018) 4:9 DOI: 10.1038/s41378-018-0012-7, WOS: 000434457800001

QC 20180601

Available from: 2018-05-04 Created: 2018-05-04 Last updated: 2019-05-08Bibliographically approved
Guo, W., Gustafsson, L., Jansson, R., Hedhammar, M. & van der Wijngaart, W. (2018). Formation of a thin-walled Spider Silk Tube on a Micromachined Scaffold. In: Proceeding of 2018 IEEE 31st International Conference on Micro Electro Mechanical Systems (MEMS): . Paper presented at 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21 January 2018 through 25 January 2018 (pp. 83-85). Institute of Electrical and Electronics Engineers (IEEE), 2018
Open this publication in new window or tab >>Formation of a thin-walled Spider Silk Tube on a Micromachined Scaffold
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2018 (English)In: Proceeding of 2018 IEEE 31st International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers (IEEE), 2018, Vol. 2018, p. 83-85Conference paper, Published paper (Refereed)
Abstract [en]

This paper reports on the first formation of a thin bio-functionalized spider silk tube, supported by an internal micromachined scaffold, in which both the inside and outside of the tube wall are freely accessible. The silk tube could potentially be used as an artificial blood vessel in an in vitro tissue scaffold, where endothelial cells and tissue cells can grow on both sides of the silk tube.

Place, publisher, year, edition, pages
Institute of Electrical and Electronics Engineers (IEEE), 2018
Series
Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), ISSN 1084-6999
Keywords
spider silk, tissue engineering, artificial blood vessel
National Category
Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
urn:nbn:se:kth:diva-225863 (URN)10.1109/MEMSYS.2018.8346488 (DOI)000434960900023 ()2-s2.0-85047021023 (Scopus ID)9781538647820 (ISBN)
Conference
31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21 January 2018 through 25 January 2018
Funder
EU, Horizon 2020, 675412Swedish Research Council, 621-2014-6200
Note

QC 20180515

Available from: 2018-04-10 Created: 2018-04-10 Last updated: 2018-12-05Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-8248-6670

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