Open this publication in new window or tab >>KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Surface and Corrosion Science.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Surface and Corrosion Science. Karolinska Inst, AIMES Ctr Advancement Integrated Med & Engn Sci, SE-10044 Stockholm, Sweden.;KTH Royal Inst Technol, SE-10044 Stockholm, Sweden.;Karolinska Inst, Dept Neurosci, SE-17177 Stockholm, Sweden..
Czech Acad Sci, Inst Microbiol, SE-68385 Prague, Czech Republic..
Örebro Univ, Fac Med & Hlth, Sch Med Sci, SE-70182 Örebro, Sweden..
Örebro Univ, Fac Med & Hlth, Inflammatory Response & Infect Susceptibil Ctr iRi, SE-70182 Örebro, Sweden.;Örebro Univ, Fac Med & Hlth, Sch Med Sci, SE-70182 Örebro, Sweden.;Örebro Univ Hosp, Dept Occupat & Environm Med, SE-70185 Örebro, Sweden..
Örebro Univ, Fac Med & Hlth, Inflammatory Response & Infect Susceptibil Ctr iRi, SE-70182 Örebro, Sweden.;Örebro Univ, Fac Med & Hlth, Sch Med Sci, SE-70182 Örebro, Sweden..
Örebro Univ, Man Technol Environm Res Ctr MTM, SE-70182 Örebro, Sweden..
Örebro Univ, Fac Med & Hlth, Inflammatory Response & Infect Susceptibil Ctr iRi, SE-70182 Örebro, Sweden.;Örebro Univ, Fac Med & Hlth, Sch Med Sci, SE-70182 Örebro, Sweden..
Örebro Univ, Man Technol Environm Res Ctr MTM, SE-70182 Örebro, Sweden.;Örebro Univ, Fac Med & Hlth, Inflammatory Response & Infect Susceptibil Ctr iRi, SE-70182 Örebro, Sweden.;Örebro Univ, Fac Med & Hlth, Sch Med Sci, SE-70182 Örebro, Sweden..
Örebro Univ, Man Technol Environm Res Ctr MTM, SE-70182 Örebro, Sweden..
Örebro Univ, Fac Med & Hlth, Sch Med Sci, SE-70182 Örebro, Sweden..
Örebro Univ, Fac Med & Hlth, Inflammatory Response & Infect Susceptibil Ctr iRi, SE-70182 Örebro, Sweden.;Örebro Univ, Fac Med & Hlth, Sch Med Sci, SE-70182 Örebro, Sweden..
Örebro Univ, Man Technol Environm Res Ctr MTM, SE-70182 Örebro, Sweden..
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2023 (English)In: Cells, E-ISSN 2073-4409, Vol. 12, no 2, article id 281Article in journal (Refereed) Published
Abstract [en]
Additive manufacturing (AM) or industrial 3D printing uses cutting-edge technologies and materials to produce a variety of complex products. However, the effects of the unintentionally emitted AM (nano)particles (AMPs) on human cells following inhalation, require further investigations. The physicochemical characterization of the AMPs, extracted from the filter of a Laser Powder Bed Fusion (L-PBF) 3D printer of iron-based materials, disclosed their complexity, in terms of size, shape, and chemistry. Cell Painting, a high-content screening (HCS) assay, was used to detect the subtle morphological changes elicited by the AMPs at the single cell resolution. The profiling of the cell morphological phenotypes, disclosed prominent concentration-dependent effects on the cytoskeleton, mitochondria, and the membranous structures of the cell. Furthermore, lipidomics confirmed that the AMPs induced the extensive membrane remodeling in the lung epithelial and macrophage co-culture cell model. To further elucidate the biological mechanisms of action, the targeted metabolomics unveiled several inflammation-related metabolites regulating the cell response to the AMP exposure. Overall, the AMP exposure led to the internalization, oxidative stress, cytoskeleton disruption, mitochondrial activation, membrane remodeling, and metabolic reprogramming of the lung epithelial cells and macrophages. We propose the approach of integrating Cell Painting with metabolomics and lipidomics, as an advanced nanosafety methodology, increasing the ability to capture the cellular and molecular phenotypes and the relevant biological mechanisms to the (nano)particle exposure.
Place, publisher, year, edition, pages
MDPI AG, 2023
Keywords
additive manufacturing, nanoparticle emissions, high-content screening (HCS), multivariate analysis, inflammation, targeted metabolomics, new approach methodologies (NAMs)
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-323769 (URN)10.3390/cells12020281 (DOI)000916977400001 ()36672217 (PubMedID)2-s2.0-85146736511 (Scopus ID)
Note
QC 20230215
2023-02-152023-02-152023-02-15Bibliographically approved