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Blomberg, E., Wang, X., Herting, G., Khort, A., Arora, A., Buxton, S., . . . Odnevall, I. (2025). Effects of sonication on particle dispersions from a size, biodissolution, cytotoxicity and transferred dose perspective – a case study on nickel and nickel oxide particles. PLOS ONE, 20(5 May), Article ID e0323368.
Open this publication in new window or tab >>Effects of sonication on particle dispersions from a size, biodissolution, cytotoxicity and transferred dose perspective – a case study on nickel and nickel oxide particles
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2025 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 20, no 5 May, article id e0323368Article in journal (Refereed) Published
Abstract [en]

The toxicity of micro- and nanoparticles in cell culture studies is influenced by factors like particle size, agglomeration, dissolution of the particles, and methodological factors like sonication protocols. The main aim of this study was to investigate the influence of sonication on the particle size, dissolution, cytotoxicity, and dosing accuracy of nickel (Ni) metal and Ni oxide (NiO) particle dispersions. Such investigations are important to enable studies on the cellular uptake of different Ni substances in lung cells. The effect of sonication was evaluated in ultrapure water, two types of cell media, and A549 human lung cells using the tip and water bath methods. Extended sonication significantly decreased particle size and increased particle dissolution, emphasizing the need for optimized sonication conditions tailored to the specific particle type and study design. Observed findings demonstrate that the sonication step potentially can have a large impact on the results due to changes in particle characteristics, size, and dissolution, properties which are highly dependent on the particle type, solution composition, and sonication parameters. Although only small differences were observed in the limited assessment of cytotoxicity (A549 cells) in this study, further investigation is required to determine the impact of sonication on toxicity. This study also emphasizes the need to evaluate transferred dose samples due to the evident effects of agglomeration, sedimentation, and losses during sample transfer of particle dispersions. The study clearly illustrates that the choice of sonication protocol is particularly critical for toxicity studies, which are the basis of government regulatory decisions and human exposure limits.

Place, publisher, year, edition, pages
Public Library of Science (PLoS), 2025
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-363786 (URN)10.1371/journal.pone.0323368 (DOI)40344085 (PubMedID)2-s2.0-105004811146 (Scopus ID)
Note

QC 20250528

Available from: 2025-05-21 Created: 2025-05-21 Last updated: 2025-05-28Bibliographically approved
Chang, T., Leygraf, C., Herting, G., Fan, Y., Babu, P., Malkoch, M., . . . Odnevall, I. (2024). Effect of blue light illumination on atmospheric corrosion and bacterial adhesion on copper. Corrosion Science, 230, Article ID 111909.
Open this publication in new window or tab >>Effect of blue light illumination on atmospheric corrosion and bacterial adhesion on copper
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2024 (English)In: Corrosion Science, ISSN 0010-938X, E-ISSN 1879-0496, Vol. 230, article id 111909Article in journal (Refereed) Published
Abstract [en]

The effect of blue light on atmospheric corrosion of Cu and on the antimicrobial properties was explored upon exposure mimicking the condition of hygienic surface disinfection. The results show that blue light illumination enhanced the formation of Cu2O, resulting in a slightly increased corrosion resistance of Cu without pre-deposited NaCl, whereas the enhanced formation of Cu2O, CuCl and/or Cu(OH)3Cl on copper with pre-deposited NaCl caused concomitant corrosion product flaking and a reduced corrosion resistance. The blue light induced enhancement of Cu corrosion led to increased surface roughness and more pronounced integration of bacteria within the corrosion products.

Place, publisher, year, edition, pages
Elsevier BV, 2024
Keywords
Atmospheric corrosion, Bacteria (E. coli), Blue light, Chloride, Copper
National Category
Surface- and Corrosion Engineering
Identifiers
urn:nbn:se:kth:diva-343995 (URN)10.1016/j.corsci.2024.111909 (DOI)001185810700001 ()2-s2.0-85185492077 (Scopus ID)
Note

QC 20240229

Available from: 2024-02-28 Created: 2024-02-28 Last updated: 2025-02-09Bibliographically approved
Chang, T., Khort, A., Saeed, A., Blomberg, E., Nielsen, M. B., Hansen, S. F. & Odnevall, I. (2023). Effects of interactions between natural organic matter and aquatic organism degradation products on the transformation and dissolution of cobalt and nickel-based nanoparticles in synthetic freshwater. Journal of Hazardous Materials, 445, 130586-130586, Article ID 130586.
Open this publication in new window or tab >>Effects of interactions between natural organic matter and aquatic organism degradation products on the transformation and dissolution of cobalt and nickel-based nanoparticles in synthetic freshwater
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2023 (English)In: Journal of Hazardous Materials, ISSN 0304-3894, E-ISSN 1873-3336, Vol. 445, p. 130586-130586, article id 130586Article in journal (Refereed) Published
Abstract [en]

Expanding applications and production of engineered nanoparticles lead to an increased risk for their environmental dispersion. Systematic knowledge of surface transformation and dissolution of nanoparticles is essential for risk assessment and regulation establishment. Such aspects of Co- and Ni-based nanoparticles including metals, oxides, and solution combustion synthesized metal nanoparticles (metal cores with carbon shells) were investigated upon environmental interaction with organic matter, simulated by natural organic matter (NOM) and degradation products from zooplankton and algae (eco-corona biomolecules, EC) in freshwater (FW). The presence of NOM and EC in FW results in negative surface charges of the nanoparticles reduces the extent of nanoparticles agglomeration, and increases concentration, mainly due to the surface adsorption of carboxylate groups of the organic matter. The dissolution of the Co-based nanoparticles was for all conditions (FW, FW with NOM or EC) higher than the Ni-based, except for Co3O4 being nearly non-soluble. The surface transformation and dissolution of nanoparticles are highly exposure and time-dependent, and surface- and environment specific. Therefore, no general correlation was observed between dissolution and, particle types, surface conditions, or EC/NOM adsorption. This underlines the importance of thorough investigations of nanoparticles adsorption/desorption, degradation, and exposure scenarios for developing regulatory relevant protocols and guidelines.

Place, publisher, year, edition, pages
Elsevier BV, 2023
Keywords
Nanoparticles, Environmental transformation, Freshwater, Natural organic matter, Eco-corona
National Category
Ecology Nano Technology Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-323171 (URN)10.1016/j.jhazmat.2022.130586 (DOI)000903963200002 ()37055991 (PubMedID)2-s2.0-85144032203 (Scopus ID)
Note

QC 20230214

Available from: 2023-01-19 Created: 2023-01-19 Last updated: 2023-06-08Bibliographically approved
Kessler, A., Huang, P., Blomberg, E. & Odnevall, I. (2023). Unravelling the Mechanistic Understanding of Metal Nanoparticle-Induced Reactive Oxygen Species Formation: Insights from a Cu Nanoparticle Study. Chemical Research in Toxicology, 36(12), 1891-1900
Open this publication in new window or tab >>Unravelling the Mechanistic Understanding of Metal Nanoparticle-Induced Reactive Oxygen Species Formation: Insights from a Cu Nanoparticle Study
2023 (English)In: Chemical Research in Toxicology, ISSN 0893-228X, E-ISSN 1520-5010, Vol. 36, no 12, p. 1891-1900Article in journal (Refereed) Published
Abstract [en]

Humans can be exposed to engineered and nonintentionally formed metal and metal oxide nanoparticles (Me NPs) in occupational settings, in public transportation areas, or by means of contact with different consumer products. A critical factor in the toxic potency of Me NPs is their ability to induce oxidative stress. It is thus essential to assess the potential reactive oxygen species (ROS) formation properties of Me NPs. A common way to assess the relative extent of ROS formation in vitro is to use fluorescence spectroscopy with the DCFH-DA (2 ',7'-dichlorofluorescein diacetate) probe, with and without HRP (horseradish peroxidase). However, this method does not provide any information about specific ROS species or reaction mechanisms. This study investigated the possibility of using complementary techniques to obtain more specific information about formed ROS species, both the type and reaction mechanisms. Cu NPs in PBS (phosphate buffered saline) were chosen as a test system to have the simplest (least interference from other components) aqueous solution with a physiologically relevant pH. ROS formation was assessed using fluorescence by means of the DCFH-DA method (information on relative amounts of oxygen radicals without selectivity), the Ghormley's triiodide method using UV-vis spectrophotometry (concentrations of H2O2), and electron paramagnetic resonance with DMPO as the spin-trap agent (information on specific oxygen radicals). This approach elucidates that Cu NPs undergo ROS-generating corrosion reactions, which previously have not been assessed in situ. In the presence of H2O2, and based on the type of oxygen radical formed, it was concluded that released copper participates in Haber-Weiss and/or Fenton reactions rather than in Fenton-like reactions. The new combination of techniques used to determine ROS induced by Me NPs provides a way forward to gain a mechanistic understanding of Me NP-induced ROS formation, which is important for gaining crucial insight into their ability to induce oxidative stress.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2023
National Category
Other Chemistry Topics
Identifiers
urn:nbn:se:kth:diva-341987 (URN)10.1021/acs.chemrestox.3c00177 (DOI)001127564200001 ()37948660 (PubMedID)2-s2.0-85178087051 (Scopus ID)
Note

QC 20240109

Available from: 2024-01-09 Created: 2024-01-09 Last updated: 2024-01-09Bibliographically approved
Khort, A., Brookman-Amissah, M., Hedberg, J., Chang, T., Mei, N., Lundberg, A., . . . Odnevall Wallinder, I. (2022). Influence of natural organic matter on the transformation of metal and metal oxide nanoparticles and their ecotoxic potency in vitro. NANOIMPACT, 25, Article ID 100386.
Open this publication in new window or tab >>Influence of natural organic matter on the transformation of metal and metal oxide nanoparticles and their ecotoxic potency in vitro
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2022 (English)In: NANOIMPACT, ISSN 2452-0748, Vol. 25, article id 100386Article in journal (Refereed) Published
Abstract [en]

Increased use and production of engineered nanoparticles (NPs) lead to an elevated risk of their diffuse dispersion into the aquatic environment and increased concern on unknown effects induced by their release into the aquatic ecosystem. An improved understanding of the environmental transformation processes of NPs of various surface characteristics is hence imperative for risk assessment and management. This study presents results on effects of natural organic matter (NOM) on the environmental transformation and dissolution of metal and metal oxide NPs of different surface and solubility properties in synthetic freshwater (FW) with and without NOM. Adsorption of NOM was evident on most of the studied NPs, except Sb and Sb2O3, which resulted in the formation of negatively charged colloids of higher stability and smaller size distribution compared with the same NPs in FW only. The dissolution rate of the NPs in the presence of NOM correlated with the strength of interactions between the carboxylate group of NOM and the particle surface, and resulted in either no (Mn, Sb, ZnO NPs), increased (Co, Sn NPs) and decreased (Ni, NiO, Sb2O3, Y2O3 NPs) levels of dissolution. One type of metal NP from each group (Mn, Ni, Sn) were investigated to assess whether observed differences in adsorption of NOM and dissolution would influence their ecotoxic potency. The results showed Mn, Ni, and Sn NPs to generate intracellular reactive oxygen species (ROS) in a time and dose-dependent manner. The extent of ROS generation in FW was similar for both Mn and Ni NPs but higher for Sn NPs. These findings are possibly related to interactions and infiltration of the NPs with the cells, which lead to redox imbalances which could induce oxidative stress and cell damage. At the same time, the presence of NOM generally reduced the intracellular ROS generation by 20-40% for the investigated NPs and also reduced cytotoxicity of Sn NPs, which can be attributed to the stronger interaction of carboxylate groups of NOM with the surface of the NPs.

Place, publisher, year, edition, pages
Elsevier BV, 2022
Keywords
Metallic nanoparticles, Freshwater, Natural organic matter, Transformation, Reactive oxygen species, Cytotoxicity
National Category
Materials Chemistry Environmental Sciences
Identifiers
urn:nbn:se:kth:diva-309800 (URN)10.1016/j.impact.2022.100386 (DOI)000754724100006 ()35559892 (PubMedID)2-s2.0-85124217500 (Scopus ID)
Note

QC 20220315

Available from: 2022-03-15 Created: 2022-03-15 Last updated: 2022-09-23Bibliographically approved
Kessler, A., Hedberg, J., Blomberg, E. & Odnevall, I. (2022). Reactive Oxygen Species Formed by Metal and Metal Oxide Nanoparticles in Physiological Media-A Review of Reactions of Importance to Nanotoxicity and Proposal for Categorization. Nanomaterials, 12(11), 1922, Article ID 1922.
Open this publication in new window or tab >>Reactive Oxygen Species Formed by Metal and Metal Oxide Nanoparticles in Physiological Media-A Review of Reactions of Importance to Nanotoxicity and Proposal for Categorization
2022 (English)In: Nanomaterials, E-ISSN 2079-4991, Vol. 12, no 11, p. 1922-, article id 1922Article, review/survey (Refereed) Published
Abstract [en]

Diffusely dispersed metal and metal oxide nanoparticles (NPs) can adversely affect living organisms through various mechanisms and exposure routes. One mechanism behind their toxic potency is their ability to generate reactive oxygen species (ROS) directly or indirectly to an extent that depends on the dose, metal speciation, and exposure route. This review provides an overview of the mechanisms of ROS formation associated with metal and metal oxide NPs and proposes a possible way forward for their future categorization. Metal and metal oxide NPs can form ROS via processes related to corrosion, photochemistry, and surface defects, as well as via Fenton, Fenton-like, and Haber-Weiss reactions. Regular ligands such as biomolecules can interact with metallic NP surfaces and influence their properties and thus their capabilities of generating ROS by changing characteristics such as surface charge, surface composition, dissolution behavior, and colloidal stability. Interactions between metallic NPs and cells and their organelles can indirectly induce ROS formation via different biological responses. H2O2 can also be generated by a cell due to inflammation, induced by interactions with metallic NPs or released metal species that can initiate Fenton(-like) and Haber-Weiss reactions forming various radicals. This review discusses these different pathways and, in addition, nano-specific aspects such as shifts in the band gaps of metal oxides and how these shifts at biologically relevant energies (similar to activation energies of biological reactions) can be linked to ROS production and indicate which radical species forms. The influences of kinetic aspects, interactions with biomolecules, solution chemistry (e.g., Cl- and pH), and NP characteristics (e.g., size and surface defects) on ROS mechanisms and formation are discussed. Categorization via four tiers is suggested as a way forward to group metal and metal oxide NPs based on the ROS reaction pathways that they may undergo, an approach that does not include kinetics or environmental variations. The criteria for the four tiers are based on the ability of the metallic NPs to induce Fenton(-like) and Haber-Weiss reactions, corrode, and interact with biomolecules and their surface catalytic properties. The importance of considering kinetic data to improve the proposed categorization is highlighted.

Place, publisher, year, edition, pages
MDPI AG, 2022
Keywords
NPs, ROS, Fenton, Haber-Weiss, corrosion, radicals, band gap, biomolecules, nanotoxicity
National Category
Organic Chemistry
Identifiers
urn:nbn:se:kth:diva-314849 (URN)10.3390/nano12111922 (DOI)000808760400001 ()35683777 (PubMedID)2-s2.0-85131162491 (Scopus ID)
Note

QC 20220627

Available from: 2022-06-27 Created: 2022-06-27 Last updated: 2023-06-08Bibliographically approved
Blomberg, E., Herting, G., Kuttuva Rajarao, G., Mehtiö, T., Uusinoka, M., Ahonen, M., . . . Odnevall, I. (2022). Weathering and Antimicrobial Properties of Laminate and Powder Coatings Containing Silver Phosphate Glass Used as High-Touch Surfaces. Sustainability, 14(12), 7102
Open this publication in new window or tab >>Weathering and Antimicrobial Properties of Laminate and Powder Coatings Containing Silver Phosphate Glass Used as High-Touch Surfaces
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2022 (English)In: Sustainability, E-ISSN 2071-1050, Vol. 14, no 12, p. 7102-Article in journal (Refereed) Published
Abstract [en]

Increasing the use of hygienic high-touch surfaces with antimicrobial properties in health care and public spaces is one way to hinder the spread of bacteria and infections. This study investigates the antimicrobial efficacy and surface reactivity of commercial laminate and powder coated surfaces treated with silver-doped phosphate glass as antimicrobial additive towards two model bacterial strains, Escherichia coli and Bacillus subtilis, in relation to surface weathering and repeated cleaning. High-touch conditions in indoor environments were simulated by different extents of pre-weathering (repeated daily cycles in relative humidity at constant temperature) and simplified fingerprint contact by depositing small droplets of artificial sweat. The results elucidate that the antimicrobial efficacy was highly bacteria dependent (Gram-positive or Gram-negative), not hampered by differences in surface weathering but influenced by the amount of silver-doped additive. No detectable amounts of silver were observed at the top surfaces, though silver was released into artificial sweat in concentrations a thousand times lower than regulatory threshold values stipulated for materials and polymers in food contact. Surface cleaning with an oxidizing chemical agent was more efficient in killing bacteria compared with an agent composed of biologically degradable constituents. Cleaning with the oxidizing agent resulted further in increased wettability and presence of residues on the surfaces, effects that were beneficial from an antimicrobial efficacy perspective.

Place, publisher, year, edition, pages
MDPI AG, 2022
National Category
Materials Engineering Public Health, Global Health and Social Medicine
Identifiers
urn:nbn:se:kth:diva-313868 (URN)10.3390/su14127102 (DOI)000816778900001 ()2-s2.0-85132766292 (Scopus ID)
Note

QC 20230328

Available from: 2022-06-13 Created: 2022-06-13 Last updated: 2025-02-20Bibliographically approved
Chang, T., Sepati, M., Herting, G., Leygraf, C., Rajarao, G. K., Butina, K., . . . Odnevall Wallinder, I. (2021). A novel methodology to study antimicrobial properties of high-touch surfaces used for indoor hygiene applications-A study on Cu metal. PLOS ONE, 16(2), Article ID e0247081.
Open this publication in new window or tab >>A novel methodology to study antimicrobial properties of high-touch surfaces used for indoor hygiene applications-A study on Cu metal
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2021 (English)In: PLOS ONE, E-ISSN 1932-6203, Vol. 16, no 2, article id e0247081Article in journal (Refereed) Published
Abstract [en]

Metal-based high-touch surfaces used for indoor applications such as doorknobs, light switches, handles and desks need to remain their antimicrobial properties even when tarnished or degraded. A novel laboratory methodology of relevance for indoor atmospheric conditions and fingerprint contact has therefore been elaborated for combined studies of both tarnishing/corrosion and antimicrobial properties of such high-touch surfaces. Cu metal was used as a benchmark material. The protocol includes pre-tarnishing/corrosion of the high touch surface for different time periods in a climatic chamber at repeated dry/wet conditions and artificial sweat deposition followed by the introduction of bacteria onto the surfaces via artificial sweat droplets. This methodology provides a more realistic and reproducible approach compared with other reported procedures to determine the antimicrobial efficiency of high-touch surfaces. It provides further a possibility to link the antimicrobial characteristics to physical and chemical properties such as surface composition, chemical reactivity, tarnishing/corrosion, surface roughness and surface wettability. The results elucidate that bacteria interactions as well as differences in extent of tarnishing can alter the physical properties (e.g. surface wettability, surface roughness) as well as the extent of metal release. The results clearly elucidate the importance to consider changes in chemical and physical properties of indoor hygiene surfaces when assessing their antimicrobial properties.

Place, publisher, year, edition, pages
Public Library of Science (PLoS), 2021
National Category
Materials Engineering
Identifiers
urn:nbn:se:kth:diva-293006 (URN)10.1371/journal.pone.0247081 (DOI)000624536800106 ()33630868 (PubMedID)2-s2.0-85102097367 (Scopus ID)
Note

QC 20210420

Available from: 2021-04-20 Created: 2021-04-20 Last updated: 2024-06-10Bibliographically approved
Kessler, A., Hedberg, J., Blomberg, E., McCarrick, S., Karlsson, H. L. & Odnevall Wallinder, I. (2021). Adsorption of horseradish peroxidase on metallic nanoparticles - effects on reactive oxygen species detection using 2 '-7 ' dichlorofluorescin diacetate. Toxicology Letters, 350, S215-S216
Open this publication in new window or tab >>Adsorption of horseradish peroxidase on metallic nanoparticles - effects on reactive oxygen species detection using 2 '-7 ' dichlorofluorescin diacetate
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2021 (English)In: Toxicology Letters, ISSN 0378-4274, E-ISSN 1879-3169, Vol. 350, p. S215-S216Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
Elsevier Ireland Ltd, 2021
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-305384 (URN)000714098000511 ()
Note

QC 20211203

Available from: 2021-12-03 Created: 2021-12-03 Last updated: 2022-06-25Bibliographically approved
Kessler, A., Hedberg, J., McCarrick, S., Karlsson, H. L., Blomberg, E. & Odnevall Wallinder, I. (2021). Adsorption of Horseradish Peroxidase on Metallic Nanoparticles: Effects on Reactive Oxygen Species Detection Using 2′,7′-Dichlorofluorescin Diacetate. Chemical Research in Toxicology, 34(6), 1481-1495
Open this publication in new window or tab >>Adsorption of Horseradish Peroxidase on Metallic Nanoparticles: Effects on Reactive Oxygen Species Detection Using 2′,7′-Dichlorofluorescin Diacetate
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2021 (English)In: Chemical Research in Toxicology, ISSN 0893-228X, E-ISSN 1520-5010, Vol. 34, no 6, p. 1481-1495Article in journal (Refereed) Published
Abstract [en]

The fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA) together with the enzyme horseradish peroxidase (HRP) is widely used in nanotoxicology to study acellular reactive oxygen species (ROS) production from nanoparticles (NPs). This study examined whether HRP adsorbs onto NPs of Mn, Ni, and Cu and if this surface process influences the extent of metal release and hence the ROS production measurements using the DCFH assay in phosphate buffered saline (PBS), saline, or Dulbecco’s modified Eagle’s medium (DMEM). Adsorption of HRP was evident onto all NPs and conditions, except for Mn NPs in PBS. The presence of HRP resulted in an increased release of copper from the Cu NPs in PBS and reduced levels of nickel from the Ni NPs in saline. Both metal ions in solution and the adsorption of HRP onto the NPs can change the activity of HRP and thus influence the ROS results. The effect of HRP on the NP reactivity was shown to be solution chemistry dependent. Most notable was the evident affinity/adsorption of phosphate toward the metal NPs, followed by a reduced adsorption of HRP, the concomitant reduction in released manganese from the Mn NPs, and increased levels of released metals from the Cu NPs in PBS. Minor effects were observed for the Ni NPs. The solution pH should be monitored since the release of metals can change the solution pH and the activity of HRP is known to be pH-dependent. It is furthermore essential that solution pH adjustments are made following the addition of NaOH during diacetyl removal of DCFH-DA. Even though not observed for the given exposure conditions of this study, released metal ions could possibly induce agglomeration or partial denaturation of HRP, which in turn could result in steric hindrance for H2O2 to reach the active site of HRP. This study further emphasizes the influence of HRP on the background kinetics, its solution dependence, and effects on measured ROS signals. Different ways of correcting for the background are highlighted, as this can result in different interpretations of generated results. The results show that adsorption of HRP onto the metal NPs influenced the extent of metal release and may, depending on the investigated system, result in either under- or overestimated ROS signals if used together with the DCFH assay. HRP should hence be used with caution when measuring ROS in the presence of reactive metallic NPs.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2021
National Category
Materials Chemistry
Identifiers
urn:nbn:se:kth:diva-296890 (URN)10.1021/acs.chemrestox.0c00430 (DOI)000664624800011 ()33856197 (PubMedID)2-s2.0-85105040573 (Scopus ID)
Note

QC 20210802

Available from: 2021-06-11 Created: 2021-06-11 Last updated: 2022-11-16Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-7496-1101

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