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Publications (10 of 36) Show all publications
Thatikonda, N., Nilebäck, L., Kempe, A., Widhe, M. & Hedhammar, M. (2018). Bioactivation of Spider Silk with Basic Fibroblast Growth Factor for in Vitro Cell Culture: A Step toward Creation of Artificial ECM. ACS Biomaterials Science and Engineering, 4(9), 3384-3396
Open this publication in new window or tab >>Bioactivation of Spider Silk with Basic Fibroblast Growth Factor for in Vitro Cell Culture: A Step toward Creation of Artificial ECM
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2018 (English)In: ACS Biomaterials Science and Engineering, ISSN 2373-9878, Vol. 4, no 9, p. 3384-3396Article in journal (Refereed) Published
Abstract [en]

Presentation of immobilized growth factors with retained bioactivity remains a challenge in the field of tissue engineering. In the present study, we propose a strategy to covalently conjugate a pleiotropic growth factor, basic fibroblast growth factor (bFGF) to a partial spider silk protein at gene level. The resulting silk-bFGF fusion protein has the propensity to self-assemble into silk-like fibers, and also surface coatings, as confirmed by quartz crystal microbalance studies. Functionality of the silk-bFGF coating to bind its cognate receptor was confirmed with surface plasmon resonance studies. As a step toward the creation of an artificial ECM, the silk-bFGF protein was mixed with FN-silk, an engineered spider silk protein with enhanced cell adhesive properties. Bioactivity of the thereby obtained combined silk was confirmed by successful culture of primary human endothelial cells on coatings and integrated within fibers, even in culture medium without supplemented growth factors. Together, these findings show that silk materials bioactivated with growth factors can be used for in vitro cell culture studies, and have potential as a tissue engineering scaffold.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
Keywords
artificial ECM, basic fibroblast growth factor, mammalian cell culture, recombinant spider silk
National Category
Biomaterials Science
Identifiers
urn:nbn:se:kth:diva-235446 (URN)10.1021/acsbiomaterials.8b00844 (DOI)000444526900025 ()2-s2.0-85052322322 (Scopus ID)
Note

QC 20180927

Available from: 2018-09-27 Created: 2018-09-27 Last updated: 2018-10-02Bibliographically approved
Gomez-Cid, L., Fuentes, L., Fernandez-Santos, M. E., Suarez-Sancho, S., Plasencia, V., Climent, A. M., . . . Aviles, F. F. (2018). Effect of spider silk matrix on cardiac tissue regeneration of mesenchymal stem cells. European Journal of Clinical Investigation, 48, 150-150
Open this publication in new window or tab >>Effect of spider silk matrix on cardiac tissue regeneration of mesenchymal stem cells
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2018 (English)In: European Journal of Clinical Investigation, ISSN 0014-2972, E-ISSN 1365-2362, Vol. 48, p. 150-150Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
WILEY, 2018
National Category
Clinical Medicine
Identifiers
urn:nbn:se:kth:diva-232264 (URN)000434100200324 ()
Note

QC 20180719

Available from: 2018-07-19 Created: 2018-07-19 Last updated: 2018-07-19Bibliographically approved
Petrou, G., Jansson, R., Högqvist, M., Hedhammar, M. & Crouzier, T. (2018). Engineering mucoadhesive silk. Paper presented at 255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA. Abstracts of Papers of the American Chemical Society, 255
Open this publication in new window or tab >>Engineering mucoadhesive silk
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2018 (English)In: Abstracts of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-240166 (URN)000435537701238 ()
Conference
255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA
Note

OC 20190110

Available from: 2019-01-11 Created: 2019-01-11 Last updated: 2019-05-10Bibliographically approved
Mittal, N., Lundell, F., Wågberg, L., Hedhammar, M. & Söderberg, D. (2018). Flow-assisted organization of nanostructured bio-based materials. Paper presented at 255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA. Abstract of Papers of the American Chemical Society, 255
Open this publication in new window or tab >>Flow-assisted organization of nanostructured bio-based materials
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2018 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-240164 (URN)000435537702785 ()
Conference
255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA
Note

QC 20190111

Available from: 2019-01-11 Created: 2019-01-11 Last updated: 2019-01-11Bibliographically approved
Guo, W., Gustafsson, L., Jansson, R., Hedhammar, M. & van der Wijngaart, W. (2018). Formation of a thin-walled Spider Silk Tube on a Micromachined Scaffold. In: Proceeding of 2018 IEEE 31st International Conference on Micro Electro Mechanical Systems (MEMS): . Paper presented at 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21 January 2018 through 25 January 2018 (pp. 83-85). Institute of Electrical and Electronics Engineers (IEEE), 2018
Open this publication in new window or tab >>Formation of a thin-walled Spider Silk Tube on a Micromachined Scaffold
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2018 (English)In: Proceeding of 2018 IEEE 31st International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers (IEEE), 2018, Vol. 2018, p. 83-85Conference paper, Published paper (Refereed)
Abstract [en]

This paper reports on the first formation of a thin bio-functionalized spider silk tube, supported by an internal micromachined scaffold, in which both the inside and outside of the tube wall are freely accessible. The silk tube could potentially be used as an artificial blood vessel in an in vitro tissue scaffold, where endothelial cells and tissue cells can grow on both sides of the silk tube.

Place, publisher, year, edition, pages
Institute of Electrical and Electronics Engineers (IEEE), 2018
Series
Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), ISSN 1084-6999
Keywords
spider silk, tissue engineering, artificial blood vessel
National Category
Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
urn:nbn:se:kth:diva-225863 (URN)10.1109/MEMSYS.2018.8346488 (DOI)000434960900023 ()2-s2.0-85047021023 (Scopus ID)9781538647820 (ISBN)
Conference
31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018, Belfast, United Kingdom, 21 January 2018 through 25 January 2018
Funder
EU, Horizon 2020, 675412Swedish Research Council, 621-2014-6200
Note

QC 20180515

Available from: 2018-04-10 Created: 2018-04-10 Last updated: 2018-12-05Bibliographically approved
Petrou, G., Jansson, R., Hogqvist, M., Erlandsson, J., Wågberg, L., Hedhammar, M. & Crouzier, T. (2018). Genetically Engineered Mucoadhesive Spider Silk. Biomacromolecules, 19(8), 3268-3279
Open this publication in new window or tab >>Genetically Engineered Mucoadhesive Spider Silk
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2018 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 19, no 8, p. 3268-3279Article in journal (Refereed) Published
Abstract [en]

Mucoadhesion is defined as the adhesion of a material to the mucus gel covering the mucous membranes. The mechanisms controlling mucoadhesion include nonspecific electrostatic interactions and specific interactions between the materials and the mucins, the heavily glycosylated proteins that form the mucus gel. Mucoadhesive materials can be used to develop mucosal wound dressings and noninvasive transmucosal drug delivery systems. Spider silk, which is strong, biocompatible, biodegradable, nontoxic, and lightweight would serve as an excellent base for the development of such materials. Here, we investigated two variants of the partial spider silk protein 4RepCT genetically engineered in order to functionalize them with mucoadhesive properties. The pLys-4RepCT variant was functionalized with six cationically charged lysines, aiming to provide nonspecific adhesion from electrostatic interactions with the anionically charged mucins, while the hGal3-4RepCT variant was genetically fused with the Human Galectin-3 Carbohydrate Recognition Domain which specifically binds the mucin glycans Gal beta 1-3GlcNAc and Gal beta 1-4GlcNAc. First, we demonstrated that coatings, fibers, meshes, and foams can be readily made from both silk variants. Measured by the adsorption of both bovine submaxillary mucin and pig gastric mucin, the newly produced silk materials showed enhanced mucin binding properties compared with materials of wild-type (4RepCT) silk. Moreover, we showed that pLys-4RepCT silk coatings bind mucins through electrostatic interactions, while hGal3-4RepCT silk coatings bind mucins through specific glycan-protein interactions. We envision that the two new mucoadhesive silk variants pLys-4RepCT and hGal3-4RepCT, alone or combined with other biofunctional silk proteins, constitute useful new building blocks for a range of silk protein-based materials for mucosal treatments.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2018
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:kth:diva-234195 (URN)10.1021/acs.biomac.8b00578 (DOI)000441852400011 ()29932649 (PubMedID)2-s2.0-85049259614 (Scopus ID)
Note

QC 20180920

Available from: 2018-09-20 Created: 2018-09-20 Last updated: 2019-04-13Bibliographically approved
Nilebäck, L., Arola, S., Kvick, M., Paananen, A., Linder, M. B. & Hedhammar, M. (2018). Interfacial Behavior of Recombinant Spider Silk Protein Parts Reveals Cues on the Silk Assembly Mechanism. Langmuir, 34(39), 11795-11805
Open this publication in new window or tab >>Interfacial Behavior of Recombinant Spider Silk Protein Parts Reveals Cues on the Silk Assembly Mechanism
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2018 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 34, no 39, p. 11795-11805Article in journal (Refereed) Published
Abstract [en]

The mechanism of silk assembly, and thus the cues for the extraordinary properties of silk, can be explored by studying the simplest protein parts needed for the formation of silk-like materials. The recombinant spider silk protein 4RepCT, consisting of four repeats of polyalanine and glycine-rich segments (4Rep) and a globular C-terminal domain (CT), has previously been shown to assemble into silk-like fibers at the liquid-air interface. Herein, we study the interfacial behavior of the two parts of 4RepCT, revealing new details on how each protein part is crucial for the silk assembly. Interfacial rheology and quartz crystal microbalance with dissipation show that 4Rep interacts readily at the interfaces. However, organized nanofibrillar structures are formed only when 4Rep is fused to CT. A strong interplay between the parts to direct the assembly is demonstrated. The presence of either a liquid-air or a liquid-solid interface had a surprisingly similar influence on the assembly.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Structural Biology
Identifiers
urn:nbn:se:kth:diva-237101 (URN)10.1021/acs.langmuir.8b02381 (DOI)000446543000016 ()30183309 (PubMedID)2-s2.0-85053916732 (Scopus ID)
Funder
Swedish Research Council FormasKnut and Alice Wallenberg Foundation
Note

QC 20181030

Available from: 2018-10-30 Created: 2018-10-30 Last updated: 2019-02-04Bibliographically approved
Horak, J., Jansson, R., Dev, A., Nilebäck, L., Behnam, K., Linnros, J., . . . Eriksson Karlström, A. (2018). Recombinant Spider Silk as Mediator for One-Step, Chemical-Free Surface Biofunctionalization. Advanced Functional Materials, 28(21), Article ID 1800206.
Open this publication in new window or tab >>Recombinant Spider Silk as Mediator for One-Step, Chemical-Free Surface Biofunctionalization
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2018 (English)In: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 28, no 21, article id 1800206Article in journal (Refereed) Published
Abstract [en]

A unique strategy for effective, versatile, and facile surface biofunctionalization employing a recombinant spider silk protein genetically functionalized with the antibody-binding Z domain (Z-4RepCT) is reported. It is demonstrated that Z-silk can be applied to a variety of materials and platform designs as a truly one-step and chemical-free surface modification that site specifically captures antibodies while simultaneously reducing nonspecific adsorption. As a model surface, SiO2 is used to optimize and characterize Z-silk performance compared to the Z domain immobilized by a standard silanization method. First, Z-silk adsorption is investigated and verified its biofunctionality in a long-term stability experiment. To assess the binding capacity and protein-protein interaction stability of Z-silk, the coating is used to capture human antibodies in various assay formats. An eightfold higher binding capacity and 40-fold lower detection limit are obtained in the immunofluorescence assay, and the complex stability of captured antibodies is shown to be improved by a factor of 20. Applicability of Z-silk to functionalize microfluidic devices is demonstrated by antibody detection in an electrokinetic microcapillary biosensor. To test Z-silk for biomarker applications, real-time detection and quantification of human immunoglobulin G are performed in a plasma sample and C1q capture from human serum using an anti-C1q antibody.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH, 2018
Keywords
biomarker, biosensing, C1q, surface biofunctionalization, Z-silk
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-231216 (URN)10.1002/adfm.201800206 (DOI)000434030500011 ()2-s2.0-85047860287 (Scopus ID)
Note

QC 20180628

Available from: 2018-06-28 Created: 2018-06-28 Last updated: 2018-11-23Bibliographically approved
Tasiopoulos, C. P., Widhe, M. & Hedhammar, M. (2018). Recombinant Spider Silk Functionalized with a Motif from Fibronectin Mediates Cell Adhesion and Growth on Polymeric Substrates by Entrapping Cells During Self-Assembly. ACS Applied Materials and Interfaces, 10(17), 14531-14539
Open this publication in new window or tab >>Recombinant Spider Silk Functionalized with a Motif from Fibronectin Mediates Cell Adhesion and Growth on Polymeric Substrates by Entrapping Cells During Self-Assembly
2018 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 10, no 17, p. 14531-14539Article in journal (Refereed) Published
Abstract [en]

In vitro endothelialization of synthetic grafts or engineered vascular constructs is considered a promising alternative to overcome shortcomings in the availability of autologous vessels and in graft complications with synthetics. A number of cell-seeding techniques have been implemented to render vascular grafts accessible for cells to attach, proliferate, and spread over the surface area. Nonetheless, seeding efficiency and the time needed for cells to adhere varies dramatically. Herein, we investigated a novel cell-seeding approach (denoted co-seeding) that enables cells to bind to a motif from fibronectin included in a recombinant spider silk protein. Entrapment of cells occurs at the same time as the silk assembles into a nanofibrillar coating on various substrates. Cell adhesion analysis showed that the technique can markedly improve cell-seeding efficiency to nonfunctionalized polystyrene surfaces, as well as establish cell attachment and growth of human dermal microvascular endothelial cells on bare polyethylene terephthalate and polytetrafluoroethylene (PTFE) substrates. Scanning electron microscopy images revealed a uniform endothelial cell layer and cell-substratum compliance with the functionalized silk protein to PTFE surfaces. The co-seeding technique holds a great promise as a method to reliably and quickly cellularize engineered vascular constructs as well as to in vitro endothelialize commercially available cardiovascular grafts.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2018
Keywords
cell seeding, recombinant spider silk, RGD binding motif, surface functionalization, revascularization applications
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-228432 (URN)10.1021/acsami.8b02647 (DOI)000431723400033 ()29641180 (PubMedID)2-s2.0-85046272837 (Scopus ID)
Note

QC 20180529

Available from: 2018-05-29 Created: 2018-05-29 Last updated: 2018-05-29Bibliographically approved
Nilebäck, L., Hedin, J., Widhe, M., Floderus, L. S., Krona, A., Bysell, H. & Hedhammar, M. (2017). Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study. Biomacromolecules, 18(3), 846-854
Open this publication in new window or tab >>Self-Assembly of Recombinant Silk as a Strategy for Chemical-Free Formation of Bioactive Coatings: A Real-Time Study
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2017 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 18, no 3, p. 846-854Article in journal (Refereed) Published
Abstract [en]

Functionalization of biomaterials with biologically active peptides can improve their performance after implantation. By genetic fusion to self-assembling proteins, the functional peptides can easily be presented on different physical formats. Herein, a chemical-free coating method based on self-assembly of the recombinant spider silk protein 4RepCT is described and used to prepare functional coatings on various biomaterial surfaces. The silk assembly was studied in real-time, revealing the occurrence of continuous assembly of silk proteins onto surfaces and the formation of nanofibrillar structures. The adsorbed amounts and viscoelastic properties were evaluated, and the coatings were shown to be stable against wash with hydrogen chloride, sodium hydroxide, and ethanol. Titanium, stainless steel, and hydroxyapatite were coated with silk fused to an antimicrobial peptide or a motif from fibronectin. Human primary cells cultured on the functional silk coatings show good cell viability and proliferation, implying the potential to improve implant performance and acceptance by the body.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2017
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-205496 (URN)10.1021/acs.biomac.6b01721 (DOI)000396379600019 ()28192654 (PubMedID)2-s2.0-85015235457 (Scopus ID)
Note

QC 20170524

Available from: 2017-05-24 Created: 2017-05-24 Last updated: 2019-02-04Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-0140-419X

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