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Vilaplana, FranciscoORCID iD iconorcid.org/0000-0003-3572-7798
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Publications (10 of 80) Show all publications
Ramos, M., Burgos, N., Barnard, A., Evans, G., Preece, J., Graz, M., . . . Jimenez, A. (2019). Agaricus bisporus and its by-products as a source of valuable extracts and bioactive compounds. Food Chemistry, 292, 176-187
Open this publication in new window or tab >>Agaricus bisporus and its by-products as a source of valuable extracts and bioactive compounds
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2019 (English)In: Food Chemistry, ISSN 0308-8146, E-ISSN 1873-7072, Vol. 292, p. 176-187Article, review/survey (Refereed) Published
Abstract [en]

Edible mushrooms constitute an appreciated nutritional source for humans due to their low caloric intake and their high content in carbohydrates, proteins, dietary fibre, phenolic compounds, polyunsaturated fatty acids, vitamins and minerals. It has been also demonstrated that mushrooms have health-promoting benefits. Cultivation of mushrooms, especially of the most common species Agaricus bisporus, represents an increasingly important food industry in Europe, but with a direct consequence in the increasing amount of by-products from their industrial production. This review focuses on collecting and critically investigating the current data on the bioactive properties of Agaricus bisporus as well as the recent research for the extraction of valuable functional molecules from this species and its by-products obtained after industrial processing. The state of the art regarding the antimicrobial, antioxidant, anti-allergenic and dietary compounds will be discussed for novel applications such as nutraceuticals, additives for food or cleaning products.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2019
Keywords
Agaricus Bisporus, By-products, Valorisation, Extraction techniques, Bioactive compounds
National Category
Food Science
Identifiers
urn:nbn:se:kth:diva-252359 (URN)10.1016/j.foodchem.2019.04.035 (DOI)000466346600025 ()31054663 (PubMedID)2-s2.0-85064443482 (Scopus ID)
Note

QC 20190724

Available from: 2019-07-24 Created: 2019-07-24 Last updated: 2019-07-24Bibliographically approved
Menzel, C., Gonzalez-Martinez, C., Chiralt, A. & Vilaplana, F. (2019). Antioxidant starch films containing sunflower hull extracts. Carbohydrate Polymers, 214, 142-151
Open this publication in new window or tab >>Antioxidant starch films containing sunflower hull extracts
2019 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 214, p. 142-151Article in journal (Refereed) Published
Abstract [en]

This study explores the preparation of antioxidant starch food packaging materials by the incorporation of valuable phenolic compounds extracted from sunflower hulls, which are an abundant by-product from food industry. The phenolic compounds were extracted with aqueous methanol and embedded into starch films. Their effect on starch films was investigated in terms of antioxidant activity, optical, thermal, mechanical, barrier properties and changes in starch molecular structure. The starch molecular structure was affected during thermal processing resulting in a decrease in molar mass, smaller amylopectin molecules and shorter amylose branches. Already 1-2% of extracts were sufficient to produce starch films with high antioxidant capacity. Higher amounts (4-6%) of extract showed the highest antioxidant activity, the lowest oxygen permeability and high stiffness and poor extensibility. The phenolic extracts affected predominantly the mechanical properties, whereas other changes could mainly be correlated to the lower glycerol content which was partially substituted by the extract.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Renewable packaging, Physical properties, Antimicrobial activity, Molecular weight, DPPH, Chlorogenic acid
National Category
Polymer Technologies
Identifiers
urn:nbn:se:kth:diva-249838 (URN)10.1016/j.carbpol.2019.03.022 (DOI)000462586900017 ()2-s2.0-85062949959 (Scopus ID)
Note

QC 20190426

Available from: 2019-04-26 Created: 2019-04-26 Last updated: 2019-04-26Bibliographically approved
Farahani, S. K., Escalante, A., Toriz, G., Vilaplana, F., Gatenholm, P., Hansson, P. & Wågberg, L. (2019). Experimental and Theoretical Evaluation of the Solubility/Insolubility Spruce Xylan (Arabino Glucuronoxylan). Biomacromolecules, 20(3), 1263-1270
Open this publication in new window or tab >>Experimental and Theoretical Evaluation of the Solubility/Insolubility Spruce Xylan (Arabino Glucuronoxylan)
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2019 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 20, no 3, p. 1263-1270Article in journal (Refereed) Published
Abstract [en]

The molecular solubility of softwood arabinoglucuronoxylan (AGX) has been thoroughly investigated, and it has been shown that the chemical and physical structures of the extracted hemicellulose are not significantly influenced by different purification steps, but a transient molecular solubility of AGX was observed in aqueous media at low concentrations (1 g/L) when the dissolved macromolecules had a hydrodynamic diameter of up to 10 nm. A phase separation was detected when the concentration was increased to 15 g/L leading to an association of the smaller molecules into fractal structures with a considerably larger diameter, even though the dispersions were still transparent to ocular inspection. Dynamic Light Scattering and Cryo-Transmission Electron Microscopy showed dimensions in the range of 1000 nm. The phase separation of the sample was further characterized by estimating the χ-interaction parameter of AGX in water using the Flory-Huggins theory, and the results supported that water is a poor solvent for AGX. This behavior is crucial when films and hydrogels based on these biopolymers are made, since the association will dramatically affect barrier and mechanical properties of films made from these materials.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019
National Category
Physical Chemistry
Identifiers
urn:nbn:se:kth:diva-248093 (URN)10.1021/acs.biomac.8b01686 (DOI)000461270500013 ()30689362 (PubMedID)2-s2.0-85061537168 (Scopus ID)
Note

QC 20190429

Available from: 2019-04-29 Created: 2019-04-29 Last updated: 2019-05-22Bibliographically approved
McKee, L. S., Martinez-Abad, A., Ruthes, A. C., Vilaplana, F. & Brumer, H. (2019). Focused Metabolism of beta-Glucans by the Soil Bacteroidetes Species Chitinophaga pinensis. Applied and Environmental Microbiology, 85(2), Article ID UNSP e02231-18.
Open this publication in new window or tab >>Focused Metabolism of beta-Glucans by the Soil Bacteroidetes Species Chitinophaga pinensis
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2019 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 85, no 2, article id UNSP e02231-18Article in journal (Refereed) Published
Abstract [en]

The genome and natural habitat of Chitinophaga pinensis suggest it has the ability to degrade a wide variety of carbohydrate-based biomass. Complementing our earlier investigations into the hydrolysis of some plant polysaccharides, we now show that C. pinensis can grow directly on spruce wood and on the fungal fruiting body. Growth was stronger on fungal material, although secreted enzyme activity was high in both cases, and all biomass-induced secretomes showed a predominance of beta-glucanase activities. We therefore conducted a screen for growth on and hydrolysis of beta-glucans isolated from different sources. Most noncrystalline beta-glucans supported good growth, with variable efficiencies of polysaccharide deconstruction and oligosaccharide uptake, depending on the polysaccharide backbone linkage. In all cases, beta-glucan was the only type of polysaccharide that was effectively hydrolyzed by secreted enzymes. This contrasts with the secretion of enzymes with a broad range of activities observed during growth on complex heteroglycans. Our findings imply a role for C. pinensis in the turnover of multiple types of biomass and suggest that the species may have two metabolic modes: a "scavenging mode," where multiple different types of glycan may be degraded, and a more "focused mode" of beta-glucan metabolism. The significant accumulation of some types of beta-gluco-oligosaccharides in growth media may be due to the lack of an appropriate transport mechanism, and we propose that this is due to the specificity of expressed polysaccharide utilization loci. We present a hypothetical model for beta-glucan metabolism by C. pinensis that suggests the potential for nutrient sharing among the microbial litter community. IMPORTANCE It is well known that the forest litter layer is inhabited by a complex microbial community of bacteria and fungi. However, while the importance of fungi in the turnover of natural biomass is well established, the role of their bacterial counterparts is less extensively studied. We show that Chitinophaga pinensis, a prominent member of an important bacterial genus, is capable of using both plant and fungal biomass as a nutrient source but is particularly effective at deconstructing dead fungal material. The turnover of dead fungus is key in natural elemental cycles in the forest. We show that C. pinensis can perform extensive degradation of this material to support its own growth while also releasing sugars that may serve as nutrients for other microbial species. Our work adds detail to an increasingly complex picture of life among the environmental microbiota.

Place, publisher, year, edition, pages
AMER SOC MICROBIOLOGY, 2019
Keywords
beta-glucan polysaccharides, bacteria, Bacteroidetes, biomass recycling, carbohydrate active enzymes, polysaccharide utilization loci
National Category
Botany
Identifiers
urn:nbn:se:kth:diva-242256 (URN)10.1128/AEM.02231-18 (DOI)000455226800019 ()30413479 (PubMedID)2-s2.0-85059797944 (Scopus ID)
Note

QC 20190130

Available from: 2019-01-30 Created: 2019-01-30 Last updated: 2019-01-30Bibliographically approved
Requena, R., Jimenez-Quero, A., Vargas, M., Moriana Torro, R., Chiralt, A. & Vilaplana, F. (2019). Integral Fractionation of Rice Husks into Bioactive Arabinoxylans, llulose Nanocrystals, and Silica Particles. ACS Sustainable Chemistry and Engineering, 7(6), 6275-6286
Open this publication in new window or tab >>Integral Fractionation of Rice Husks into Bioactive Arabinoxylans, llulose Nanocrystals, and Silica Particles
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2019 (English)In: ACS Sustainable Chemistry and Engineering, ISSN 2168-0485, Vol. 7, no 6, p. 6275-6286Article in journal (Refereed) Published
Abstract [en]

Rice husk is an important agricultural by-product that has not been exploited yet to full capacity for advanced applications. The feasibility of obtaining high-value products such as bioactive hemicelluloses and cellulose nanocrystals (CNCs) from rice husk is here demonstrated in a cascade biorefinery process using subcritical water extraction (SWE) prior to bleaching and acid hydrolysis and compared to traditional alkali pretreatments. The proposed SWE process enables the isolation of bioactive arabinoxylans with phenolic acid moieties, thus preserving their antioxidant and anti- bacterial properties that are lost during alkaline conditions. Bioactive Additionally, SWE can be combined with subsequent arabinoxylan Silica particles bleaching and acid hydrolysis to obtain CNCs with large aspect ratio, high crystallinity, and thermal stability. The hydrothermal process also enables the recovery of silica particles that are lost during the alkali step but can be recovered after the isolation of the CNCs. Our biorefinery strategy results in the integral valorization of rice husk into their molecular components (bioactive arabinoxylans, cellulose nanocrystals, and silica particles), which can be used as additives for food applications and as reinforcing agents in biocomposite materials, respectively.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019
Keywords
Rice husk, Subcritical water extraction, Xylans, Cellulose nanocrystals, Biorefinery
National Category
Other Chemistry Topics
Identifiers
urn:nbn:se:kth:diva-248332 (URN)10.1021/acssuschemeng.8b06692 (DOI)000461978200074 ()2-s2.0-85062832429 (Scopus ID)
Note

QC 20190410

Available from: 2019-04-10 Created: 2019-04-10 Last updated: 2019-04-10Bibliographically approved
Sullivan, M. A., Nitschke, S., Skwara, E. P., Wang, P., Zhao, X., Pan, X. S., . . . Nitschke, F. (2019). Skeletal Muscle Glycogen Chain Length Correlates with Insolubility in Mouse Models of Polyglucosan-Associated Neurodegenerative Diseases. Cell reports, 27(5), 1334-1344.e6
Open this publication in new window or tab >>Skeletal Muscle Glycogen Chain Length Correlates with Insolubility in Mouse Models of Polyglucosan-Associated Neurodegenerative Diseases
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2019 (English)In: Cell reports, ISSN 2211-1247, E-ISSN 2211-1247, Vol. 27, no 5, p. 1334-1344.e6Article in journal (Refereed) Published
Abstract [en]

Lafora disease (LD) and adult polyglucosan body disease (APBD) are glycogen storage diseases characterized by a pathogenic buildup of insoluble glycogen. Mechanisms causing glycogen insolubility are poorly understood. Here, in two mouse models of LD (Epm2a(-/-) and Epm2b(-/-)) and one of APBD (Gbe1(ys/ys)), the separation of soluble and insoluble muscle glycogen is described, enabling separate analysis of each fraction. Total glycogen is increased in LD and APBD mice, which, together with abnormal chain length and molecule size distributions, is largely if not fully attributed to insoluble glycogen. Soluble glycogen consists of molecules with distinct chain length distributions and differential corresponding solubility, providing a mechanistic link between soluble and insoluble glycogen in vivo. Phosphorylation states differ across glycogen fractions and mouse models, demonstrating that hyperphosphorylation is not a basic feature of insoluble glycogen. Lastly, model-specific variances in protein and activity levels of key glycogen synthesis enzymes suggest uninvestigated regulatory mechanisms.

Place, publisher, year, edition, pages
cell press, 2019
National Category
Cell Biology
Identifiers
urn:nbn:se:kth:diva-251707 (URN)10.1016/j.celrep.2019.04.017 (DOI)000466484100003 ()31042462 (PubMedID)2-s2.0-85064620718 (Scopus ID)
Note

QC 20190520

Available from: 2019-05-20 Created: 2019-05-20 Last updated: 2019-05-20Bibliographically approved
de Jesus, L. I., Smiderle, F. R., Ruthes, A. C., Vilaplana, F., Dal'Lin, F. T., Maria-Ferreira, D., . . . Iacomini, M. (2018). Chemical characterization and wound healing property of a beta-D-glucan from edible mushroom Piptoporus betulinus. International Journal of Biological Macromolecules, 117, 1361-1366
Open this publication in new window or tab >>Chemical characterization and wound healing property of a beta-D-glucan from edible mushroom Piptoporus betulinus
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2018 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 117, p. 1361-1366Article in journal (Refereed) Published
Abstract [en]

A water-soluble beta-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 x 10(5) Da with a random coil conformation for molecular weights below 1 x 10(6) Da. Typical signals of beta-(1 -> 3)-linkages were observed in NMR spectrum (delta 102.7/4.76; 102.8/4.74; 102.9/4.52; and delta 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at delta 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a beta-D-glucan with a main chain (1 -> 3)-linked, substituted at O-6 by single-units of glucose. The beta-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 mu g mL(-1) and promoted cell migration on in vitro scratch assay, demonstrating a potential wound healing capacity.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
beta-D-glucan, Random coil, Wound healing
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-234561 (URN)10.1016/j.ijbiomac.2017.12.107 (DOI)000442057700154 ()29274425 (PubMedID)2-s2.0-85042161770 (Scopus ID)
Note

QC 20180919

Available from: 2018-09-19 Created: 2018-09-19 Last updated: 2018-11-13Bibliographically approved
Martinez-Abad, A., Giummarella, N., Lawoko, M. & Vilaplana, F. (2018). Differences in extractability under subcritical water reveal interconnected hemicellulose and lignin recalcitrance in birch hardwoods. Green Chemistry
Open this publication in new window or tab >>Differences in extractability under subcritical water reveal interconnected hemicellulose and lignin recalcitrance in birch hardwoods
2018 (English)In: Green Chemistry, ISSN 1463-9262, E-ISSN 1463-9270Article in journal (Refereed) Published
Abstract [en]

Hardwoods constitute an essential renewable resource for the production of platform chemicals and bio-based materials. A method for the sequential extraction of hemicelluloses and lignin from hardwoods is proposed using subcritical water in buffered conditions without prior delignification. This allows the cascade isolation of mannan, xylan and lignin-carbohydrate complexes based on their extractability and recalcitrance in birch lignocellulose. The time evolution of the extraction was monitored in terms of composition, oligomeric mass profiling and sequencing of the hemicelluloses, and molecular structure of the lignin and lignin-carbohydrate complexes (LCCs) by heteronuclear single quantum coherence nuclear magnetic resonance (2D HSQC NMR). The minor mannan and pectin populations are easily extractable at short times (<5 min), whereas the major glucuronoxylan (GX) becomes enriched at moderate extraction times. Longer extraction times results in major hydrolysis exhibiting GX fractions with tighter glucuronation spacing and lignin enrichment. The pattern of acetylation and glucuronation in GX is correlated with extractability and with connectivity with lignin through LCCs. This interconnected molecular heterogeneity of hemicelluloses and lignin has important implications for their supramolecular assembly and therefore determines the recalcitrance of hardwood lignocellulosic biomass.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2018
National Category
Wood Science
Research subject
Fibre and Polymer Science
Identifiers
urn:nbn:se:kth:diva-226948 (URN)10.1039/C8GC00385H (DOI)000434313100016 ()2-s2.0-85048032938 (Scopus ID)
Funder
Knut and Alice Wallenberg Foundation
Note

QC 20180509

Available from: 2018-04-29 Created: 2018-04-29 Last updated: 2018-06-27Bibliographically approved
Quero, A. J., Rudjito, R. C., Martinez-Abad, A., Ruthes, A. C. & Vilaplana, F. (2018). Extraction of distinct populations of bioactive arabinoxylans from wheat bran using sequential chemo-enzymatic processes. Paper presented at 255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA. Abstract of Papers of the American Chemical Society, 255
Open this publication in new window or tab >>Extraction of distinct populations of bioactive arabinoxylans from wheat bran using sequential chemo-enzymatic processes
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2018 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-240168 (URN)000435537702805 ()
Conference
255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA
Note

QC 20190111

Available from: 2019-01-11 Created: 2019-01-11 Last updated: 2019-01-11Bibliographically approved
Martinez-Abad, A., Quero, A. J., Berglund, J., Giummarella, N., Henriksson, G., Lindström, M., . . . Vilaplana, F. (2018). Influence of the molecular structure of wood hemicelluloses on the recalcitrance of lignocellulosic biomass. Paper presented at 255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA. Abstract of Papers of the American Chemical Society, 255
Open this publication in new window or tab >>Influence of the molecular structure of wood hemicelluloses on the recalcitrance of lignocellulosic biomass
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2018 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-240163 (URN)000435537702769 ()
Conference
255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA
Note

QC 20190111

Available from: 2019-01-11 Created: 2019-01-11 Last updated: 2019-01-11Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0003-3572-7798

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