Change search
Link to record
Permanent link

Direct link
BETA
Vilaplana, FranciscoORCID iD iconorcid.org/0000-0003-3572-7798
Alternative names
Publications (10 of 73) Show all publications
McKee, L. S., Martinez-Abad, A., Ruthes, A. C., Vilaplana, F. & Brumer, H. (2019). Focused Metabolism of beta-Glucans by the Soil Bacteroidetes Species Chitinophaga pinensis. Applied and Environmental Microbiology, 85(2), Article ID UNSP e02231-18.
Open this publication in new window or tab >>Focused Metabolism of beta-Glucans by the Soil Bacteroidetes Species Chitinophaga pinensis
Show others...
2019 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 85, no 2, article id UNSP e02231-18Article in journal (Refereed) Published
Abstract [en]

The genome and natural habitat of Chitinophaga pinensis suggest it has the ability to degrade a wide variety of carbohydrate-based biomass. Complementing our earlier investigations into the hydrolysis of some plant polysaccharides, we now show that C. pinensis can grow directly on spruce wood and on the fungal fruiting body. Growth was stronger on fungal material, although secreted enzyme activity was high in both cases, and all biomass-induced secretomes showed a predominance of beta-glucanase activities. We therefore conducted a screen for growth on and hydrolysis of beta-glucans isolated from different sources. Most noncrystalline beta-glucans supported good growth, with variable efficiencies of polysaccharide deconstruction and oligosaccharide uptake, depending on the polysaccharide backbone linkage. In all cases, beta-glucan was the only type of polysaccharide that was effectively hydrolyzed by secreted enzymes. This contrasts with the secretion of enzymes with a broad range of activities observed during growth on complex heteroglycans. Our findings imply a role for C. pinensis in the turnover of multiple types of biomass and suggest that the species may have two metabolic modes: a "scavenging mode," where multiple different types of glycan may be degraded, and a more "focused mode" of beta-glucan metabolism. The significant accumulation of some types of beta-gluco-oligosaccharides in growth media may be due to the lack of an appropriate transport mechanism, and we propose that this is due to the specificity of expressed polysaccharide utilization loci. We present a hypothetical model for beta-glucan metabolism by C. pinensis that suggests the potential for nutrient sharing among the microbial litter community. IMPORTANCE It is well known that the forest litter layer is inhabited by a complex microbial community of bacteria and fungi. However, while the importance of fungi in the turnover of natural biomass is well established, the role of their bacterial counterparts is less extensively studied. We show that Chitinophaga pinensis, a prominent member of an important bacterial genus, is capable of using both plant and fungal biomass as a nutrient source but is particularly effective at deconstructing dead fungal material. The turnover of dead fungus is key in natural elemental cycles in the forest. We show that C. pinensis can perform extensive degradation of this material to support its own growth while also releasing sugars that may serve as nutrients for other microbial species. Our work adds detail to an increasingly complex picture of life among the environmental microbiota.

Place, publisher, year, edition, pages
AMER SOC MICROBIOLOGY, 2019
Keywords
beta-glucan polysaccharides, bacteria, Bacteroidetes, biomass recycling, carbohydrate active enzymes, polysaccharide utilization loci
National Category
Botany
Identifiers
urn:nbn:se:kth:diva-242256 (URN)10.1128/AEM.02231-18 (DOI)000455226800019 ()30413479 (PubMedID)2-s2.0-85059797944 (Scopus ID)
Note

QC 20190130

Available from: 2019-01-30 Created: 2019-01-30 Last updated: 2019-01-30Bibliographically approved
de Jesus, L. I., Smiderle, F. R., Ruthes, A. C., Vilaplana, F., Dal'Lin, F. T., Maria-Ferreira, D., . . . Iacomini, M. (2018). Chemical characterization and wound healing property of a beta-D-glucan from edible mushroom Piptoporus betulinus. International Journal of Biological Macromolecules, 117, 1361-1366
Open this publication in new window or tab >>Chemical characterization and wound healing property of a beta-D-glucan from edible mushroom Piptoporus betulinus
Show others...
2018 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 117, p. 1361-1366Article in journal (Refereed) Published
Abstract [en]

A water-soluble beta-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 x 10(5) Da with a random coil conformation for molecular weights below 1 x 10(6) Da. Typical signals of beta-(1 -> 3)-linkages were observed in NMR spectrum (delta 102.7/4.76; 102.8/4.74; 102.9/4.52; and delta 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at delta 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a beta-D-glucan with a main chain (1 -> 3)-linked, substituted at O-6 by single-units of glucose. The beta-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 mu g mL(-1) and promoted cell migration on in vitro scratch assay, demonstrating a potential wound healing capacity.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
beta-D-glucan, Random coil, Wound healing
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-234561 (URN)10.1016/j.ijbiomac.2017.12.107 (DOI)000442057700154 ()29274425 (PubMedID)2-s2.0-85042161770 (Scopus ID)
Note

QC 20180919

Available from: 2018-09-19 Created: 2018-09-19 Last updated: 2018-11-13Bibliographically approved
Martinez-Abad, A., Giummarella, N., Lawoko, M. & Vilaplana, F. (2018). Differences in extractability under subcritical water reveal interconnected hemicellulose and lignin recalcitrance in birch hardwoods. Green Chemistry
Open this publication in new window or tab >>Differences in extractability under subcritical water reveal interconnected hemicellulose and lignin recalcitrance in birch hardwoods
2018 (English)In: Green Chemistry, ISSN 1463-9262, E-ISSN 1463-9270Article in journal (Refereed) Published
Abstract [en]

Hardwoods constitute an essential renewable resource for the production of platform chemicals and bio-based materials. A method for the sequential extraction of hemicelluloses and lignin from hardwoods is proposed using subcritical water in buffered conditions without prior delignification. This allows the cascade isolation of mannan, xylan and lignin-carbohydrate complexes based on their extractability and recalcitrance in birch lignocellulose. The time evolution of the extraction was monitored in terms of composition, oligomeric mass profiling and sequencing of the hemicelluloses, and molecular structure of the lignin and lignin-carbohydrate complexes (LCCs) by heteronuclear single quantum coherence nuclear magnetic resonance (2D HSQC NMR). The minor mannan and pectin populations are easily extractable at short times (<5 min), whereas the major glucuronoxylan (GX) becomes enriched at moderate extraction times. Longer extraction times results in major hydrolysis exhibiting GX fractions with tighter glucuronation spacing and lignin enrichment. The pattern of acetylation and glucuronation in GX is correlated with extractability and with connectivity with lignin through LCCs. This interconnected molecular heterogeneity of hemicelluloses and lignin has important implications for their supramolecular assembly and therefore determines the recalcitrance of hardwood lignocellulosic biomass.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2018
National Category
Wood Science
Research subject
Fibre and Polymer Science
Identifiers
urn:nbn:se:kth:diva-226948 (URN)10.1039/C8GC00385H (DOI)000434313100016 ()2-s2.0-85048032938 (Scopus ID)
Funder
Knut and Alice Wallenberg Foundation
Note

QC 20180509

Available from: 2018-04-29 Created: 2018-04-29 Last updated: 2018-06-27Bibliographically approved
Quero, A. J., Rudjito, R. C., Martinez-Abad, A., Ruthes, A. C. & Vilaplana, F. (2018). Extraction of distinct populations of bioactive arabinoxylans from wheat bran using sequential chemo-enzymatic processes. Paper presented at 255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA. Abstract of Papers of the American Chemical Society, 255
Open this publication in new window or tab >>Extraction of distinct populations of bioactive arabinoxylans from wheat bran using sequential chemo-enzymatic processes
Show others...
2018 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-240168 (URN)000435537702805 ()
Conference
255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA
Note

QC 20190111

Available from: 2019-01-11 Created: 2019-01-11 Last updated: 2019-01-11Bibliographically approved
Martinez-Abad, A., Quero, A. J., Berglund, J., Giummarella, N., Henriksson, G., Lindström, M., . . . Vilaplana, F. (2018). Influence of the molecular structure of wood hemicelluloses on the recalcitrance of lignocellulosic biomass. Paper presented at 255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA. Abstract of Papers of the American Chemical Society, 255
Open this publication in new window or tab >>Influence of the molecular structure of wood hemicelluloses on the recalcitrance of lignocellulosic biomass
Show others...
2018 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-240163 (URN)000435537702769 ()
Conference
255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA
Note

QC 20190111

Available from: 2019-01-11 Created: 2019-01-11 Last updated: 2019-01-11Bibliographically approved
Henriksson, G., Berglund, J., Wohlert, J., Lawoko, M., Aminzadeh, S., Lindström, M. & Vilaplana, F. (2018). Non-cellulose wood polysaccharides - a need for a stricter structural and functional classification?. Paper presented at 255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA. Abstract of Papers of the American Chemical Society, 255
Open this publication in new window or tab >>Non-cellulose wood polysaccharides - a need for a stricter structural and functional classification?
Show others...
2018 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-240158 (URN)000435537702766 ()
Conference
255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA
Note

QC 20190108

Available from: 2019-01-08 Created: 2019-01-08 Last updated: 2019-01-08Bibliographically approved
Imre, B., Lidia, G., Puglia, D. & Vilaplana, F. (2018). Reactive Compatibilization of Plant Polysaccharidesand Biobased Polymers: Review on Current Strategies,Expectations and Reality. Carbohydrate Polymers
Open this publication in new window or tab >>Reactive Compatibilization of Plant Polysaccharidesand Biobased Polymers: Review on Current Strategies,Expectations and Reality
2018 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344Article in journal (Refereed) Published
Abstract [en]

Our society is amidst a technological revolution towards a sustainable economy, focused on the development of biobased products in virtually all sectors. In this context, plant polysaccharides, as the most abundant macromoleculespresent in biomass represent a fundamental renewable resource for the replacement of fossil-based polymeric materials in commodity and engineering applications. However, native polysaccharides have several disadvantages compared to their synthetic counterparts, including reduced thermal stability, moisture absorption and limited mechanical performance, which hinder their direct application in native form in advanced material systems. Thus, polysaccharides are generally used in a derivatized form and/or in combination with other biobased polymers, requiring the compatibilization of such blends and composites. In this review we critically explore the current status and the future outlook of reactive compatibilization strategies of the most common plant polysaccharides in blends with biobased polymers. The chemical processes for the modification and compatibilization of starch and lignocellulosic based materialsare discussed, together with the practical implementation of these reactive compatibilization strategies with special emphasis on reactive extrusion. The efficiency of these strategies is critically discussed in the context on the definition of blending and compatibilization from a polymer physics standpoint; this relies on the detailed evaluation of the chemical structure of the constituent plant polysaccharides and biobased polymers, the morphology of the heterogeneous polymeric blends, and their macroscopic behavior, in terms of rheological and mechanical properties.

Place, publisher, year, edition, pages
Elsevier, 2018
National Category
Engineering and Technology Polymer Technologies
Research subject
Materials Science and Engineering
Identifiers
urn:nbn:se:kth:diva-241052 (URN)10.1016/j.carbpol.2018.12.082 (DOI)000457546200003 ()2-s2.0-85059673274 (Scopus ID)
Note

QC 20190108

Available from: 2019-01-07 Created: 2019-01-07 Last updated: 2019-02-20Bibliographically approved
Kishani, S., Wohlert, J., Vilaplana, F. & Wågberg, L. (2018). Solubility and adsorption of different xyloglucan fractions to model surfaces. Paper presented at 255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA. Abstract of Papers of the American Chemical Society, 255
Open this publication in new window or tab >>Solubility and adsorption of different xyloglucan fractions to model surfaces
2018 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 255Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-240161 (URN)000435537702811 ()
Conference
255th National Meeting and Exposition of the American-Chemical-Society (ACS) - Nexus of Food, Energy, and Water, MAR 18-22, 2018, New Orleans, LA
Note

QC 20190110

Available from: 2019-01-10 Created: 2019-01-10 Last updated: 2019-01-10Bibliographically approved
Jamshidian, H., Shojaosadati, S. A., Mousavi, S. M., Soudi, M. R. & Vilaplana, F. (2017). Implications of recovery procedures on structural and rheological properties of schizophyllan produced from date syrup. International Journal of Biological Macromolecules, 105, 36-44
Open this publication in new window or tab >>Implications of recovery procedures on structural and rheological properties of schizophyllan produced from date syrup
Show others...
2017 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 105, p. 36-44Article in journal (Refereed) Published
Abstract [en]

This study investigates the effects of different recovery procedures on high molar mass schizophyl-Ian produced by Schizophyllum commune using low value agricultural residues. Recovered extracellular polysaccharides (EPSs) were compared in terms of purity, sugar composition, degree of branching, molecular weight, and rheological properties. Performing different recovery methods, such as re-dissolving in water and re-precipitation with ethanol on produced EPS, provided schizophyllan with purity similar to the commercial grade. Besides, Freeze-thawing cycles allowed the fractionation of schizophyllan based on branching degree and solubility. The EPSs with higher purity and lower degree of branching (less conformational flexibility) showed higher viscosity. This study evidences the possibility of producing EPSs with excellent rheological properties using low value agricultural side products. Furthermore, our results demonstrate the importance of recovery methods for tailoring the purity, molecular structure and macroscopic properties of the produced polysaccharides for specific applications.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
Schizophyllan, Exopolysaccharide (EPS), Recovery procedure, Rheological properties, Molecular structures
National Category
Biochemistry and Molecular Biology Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-220267 (URN)10.1016/j.ijbiomac.2017.06.110 (DOI)000414882900005 ()28668611 (PubMedID)2-s2.0-85023600986 (Scopus ID)
Note

QC 20180110

Available from: 2018-01-10 Created: 2018-01-10 Last updated: 2018-01-10Bibliographically approved
Morais de Carvalho, D., Abad, A. M., Evtuguin, D. V., Colodette, J. L., Lindström, M., Vilaplana, F. & Sevastyanova, O. (2017). Isolation and characterization of acetylated glucuronoarabinoxylan from sugarcane bagasse and straw. Carbohydrate Polymers, 156, 223-234
Open this publication in new window or tab >>Isolation and characterization of acetylated glucuronoarabinoxylan from sugarcane bagasse and straw
Show others...
2017 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 156, p. 223-234Article in journal (Refereed) Published
Abstract [en]

Sugarcane bagasse and straw are generated in large volumes as by-products of agro-industrial production. They are an emerging valuable resource for the generation of hemicellulose-based materials and products, since they contain significant quantities of xylans (often twice as much as in hardwoods). Heteroxylans (yields of ca 20% based on xylose content in sugarcane bagasse and straw) were successfully isolated and purified using mild delignification followed by dimethyl sulfoxide (DMSO) extraction. Delignification with peracetic acid (PAA) was more efficient than traditional sodium chlorite (NaClO2) delignification for xylan extraction from both biomasses, resulting in higher extraction yields and purity. We have shown that the heteroxylans isolated from sugarcane bagasse and straw are acetylated glucuronoarabinoxylans (GAX), with distinct molecular structures. Bagasse GAX had a slightly lower glycosyl substitution molar ratio of Araf to Xylp to (0.5:10) and (4-O-Me)GlpA to Xylp (0.1:10) than GAX from straw (0.8:10 and 0.1:10 respectively), but a higher degree of acetylation (0.33 and 0.10, respectively). A higher frequency of acetyl groups substitution at position α-(1 → 3) (Xyl-3Ac) than at position α-(1 → 2) (Xyl-2Ac) was confirmed for both bagasse and straw GAX, with a minor ratio of diacetylation (Xyl-2,3Ac). The size and molecular weight distributions for the acetylated GAX extracted from the sugarcane bagasse and straw were analyzed using multiple-detection size-exclusion chromatography (SEC-DRI-MALLS). Light scattering data provided absolute molar mass values for acetylated GAX with higher average values than did standard calibration. Moreover, the data highlighted differences in the molar mass distributions between the two isolation methods for both types of sugarcane GAX, which can be correlated with the different Araf and acetyl substitution patterns. We have developed an empirical model for the molecular structure of acetylated GAX extracted from sugarcane bagasse and straw with PAA/DMSO through the integration of results obtained from glycosidic linkage analysis, 1H NMR spectroscopy and acetyl quantification. This knowledge of the structure of xylans in sugarcane bagasse and straw will provide a better understanding of the isolation-structure-properties relationship of these biopolymers and, ultimately, create new possibilities for the use of sugarcane xylan in high-value applications, such as biochemicals and bio-based materials. © 2016 Elsevier Ltd

Keywords
1H NMR spectroscopy, Acetylated xylan, Arabinoxylan, Linkage analysis, Sugarcane bagasse, Sugarcane straw, Acetylation, Biopolymers, Characterization, Delignification, Dimethyl sulfoxide, Extraction, Light scattering, Molecular structure, Molecular weight distribution, Nuclear magnetic resonance spectroscopy, Organic solvents, Polysaccharides, Size exclusion chromatography, Arabinoxylans, H NMR spectroscopy, Sugar-cane bagasse, Bagasse
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-195116 (URN)10.1016/j.carbpol.2016.09.022 (DOI)2-s2.0-84987851359 (Scopus ID)
Note

Funding Details: 621-2014-5295, VR, Swedish Research Council. QC 20161107

Available from: 2016-11-07 Created: 2016-11-02 Last updated: 2017-06-28Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-3572-7798

Search in DiVA

Show all publications