Change search
Link to record
Permanent link

Direct link
BETA
Finne Wistrand, AnnaORCID iD iconorcid.org/0000-0002-1922-128X
Alternative names
Publications (10 of 100) Show all publications
Yassin, M. A., Fuoco, T., Mohamed-Ahmed, S., Mustafa, K. & Finne Wistrand, A. (2019). 3D and Porous RGDC-Functionalized Polyester-Based Scaffolds as a Niche to Induce Osteogenic Differentiation of Human Bone Marrow Stem Cells. Macromolecular Bioscience, 19(6), Article ID 1900049.
Open this publication in new window or tab >>3D and Porous RGDC-Functionalized Polyester-Based Scaffolds as a Niche to Induce Osteogenic Differentiation of Human Bone Marrow Stem Cells
Show others...
2019 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 19, no 6, article id 1900049Article in journal (Refereed) Published
Abstract [en]

Polyester-based scaffolds covalently functionalized with arginine-glycine-aspartic acid-cysteine (RGDC) peptide sequences support the proliferation and osteogenic differentiation of stem cells. The aim is to create an optimized 3D niche to sustain human bone marrow stem cell (hBMSC) viability and osteogenic commitment, without reliance on differentiation media. Scaffolds consisting of poly(lactide-co-trimethylene carbonate), poly(LA-co-TMC), and functionalized poly(lactide) copolymers with pendant thiol groups are prepared by salt-leaching technique. The availability of functional groups on scaffold surfaces allows for an easy and straightforward method to covalently attach RGDC peptide motifs without affecting the polymerization degree. The strategy enables the chemical binding of bioactive motifs on the surfaces of 3D scaffolds and avoids conventional methods that require harsh conditions. Gene and protein levels and mineral deposition indicate the osteogenic commitment of hBMSC cultured on the RGDC functionalized surfaces. The osteogenic commitment of hBMSC is enhanced on functionalized surfaces compared with nonfunctionalized surfaces and without supplementing media with osteogenic factors. Poly(LA-co-TMC) scaffolds have potential as scaffolds for osteoblast culture and bone grafts. Furthermore, these results contribute to the development of biomimetic materials and allow a deeper comprehension of the importance of RGD peptides on stem cell transition toward osteoblastic lineage.

Place, publisher, year, edition, pages
WILEY-V C H VERLAG GMBH, 2019
Keywords
degradable polymer, human bone marrow stem cells, poly(l-lactide-co-trimethylene carbonate), RGDC
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-255207 (URN)10.1002/mabi.201900049 (DOI)000471782900010 ()31050389 (PubMedID)2-s2.0-85065316726 (Scopus ID)
Note

QC 20190904

Available from: 2019-09-04 Created: 2019-09-04 Last updated: 2019-09-04Bibliographically approved
Pappalardo, D., Mathisen, T. & Finne Wistrand, A. (2019). Biocompatibility of Resorbable Polymers: A Historical Perspective and Framework for the Future. Biomacromolecules, 20(4), 1465-1477
Open this publication in new window or tab >>Biocompatibility of Resorbable Polymers: A Historical Perspective and Framework for the Future
2019 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 20, no 4, p. 1465-1477Article in journal (Refereed) Published
Abstract [en]

The history of resorbable polymers containing glycolide, lactide, e-caprolactone and trimethylene carbonate, with a special emphasis being placed on the time frame of the 1960s-1990s is described. Reviewing the history is valuable when looking into the future perspectives regarding how and where these monomers should be used. This story includes scientific evaluations indicating that these polymers are safe to use in medical devices, while the design of the medical device is not considered in this report. In particular, we present the data regarding the tissue response to implanted polymers, as well as the toxicity and pharmacokinetics of their degradation products. In the translation of these polymers from "the bench to the bedside," various challenges have been faced by surgeons, medical doctors, biologists, material engineers and polymer chemists. This Perspective highlights the visionary role played by the pioneers, addressing the problems that occurred on a case by case basis in translational medicine.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2019
Keywords
NDUNNEN WFA, 1993, JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, V4, P521 nmark S., 2011, ACTA BIOMATERIALIA, V7, P2035 go AP, 2003, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, V67A, P1044, NDUNNEN WFA, 1993, MICROSURGERY, V14, P508 go AP, 2002, MACROMOLECULAR BIOSCIENCE, V2, P411, NDUNNEN WFA, 1995, JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, V29, P757, LGUERRA RS, 1994, JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, V5, P891 e of International Standard., 2016, ISO 109931, ndgren D., 1994, J. Swed. Dent. Assoc, V65, P967
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-251216 (URN)10.1021/acs.biomac.9b00159 (DOI)000464248300001 ()30855137 (PubMedID)2-s2.0-85064110982 (Scopus ID)
Note

QC 20190524

Available from: 2019-05-24 Created: 2019-05-24 Last updated: 2019-05-24Bibliographically approved
Munir, A., Doskeland, A., Avery, S. J., Fuoco, T., Mohamed-Ahmed, S., Lygre, H., . . . Suliman, S. (2019). Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration. Journal of Tissue Engineering, 10, Article ID 2041731419852703.
Open this publication in new window or tab >>Efficacy of copolymer scaffolds delivering human demineralised dentine matrix for bone regeneration
Show others...
2019 (English)In: Journal of Tissue Engineering, ISSN 2041-7314, E-ISSN 2041-7314, Vol. 10, article id 2041731419852703Article in journal (Refereed) Published
Abstract [en]

Poly(L-lactide-co-epsilon-caprolactone) scaffolds were functionalised by 10 or 20 mu g/mL of human demineralised dentine matrix. Release kinetics up to 21 days and their osteogenic potential on human bone marrow stromal cells after 7 and 21 days were studied. A total of 390 proteins were identified by mass spectrometry. Bone regeneration proteins showed initial burst of release. Human bone marrow stromal cells were cultured on scaffolds physisorbed with 20 mu g/mL and cultured in basal medium (DDM group) or physisorbed and cultured in osteogenic medium or cultured on non-functionalised scaffolds in osteogenic medium. The human bone marrow stromal cells proliferated less in demineralised dentine matrix group and activated ERK/1/2 after both time points. Cells on DDM group showed highest expression of IL-6 and IL-8 at 7 days and expressed higher collagen type 1 alpha 2, SPP1 and bone morphogenetic protein-2 until 21 days. Extracellular protein revealed higher collagen type 1 and bone morphogenetic protein-2 at 21 days in demineralised dentine matrix group. Cells on DDM group showed signs of mineralisation. The functionalised scaffolds were able to stimulate osteogenic differentiation of human bone marrow stromal cells.

Place, publisher, year, edition, pages
SAGE PUBLICATIONS INC, 2019
Keywords
Growth factor, mesenchymal stem cell, bone tissue engineering, drug delivery, functionalisation
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-254028 (URN)10.1177/2041731419852703 (DOI)000469789400001 ()2-s2.0-85066462356 (Scopus ID)
Note

QC 20190814

Available from: 2019-08-14 Created: 2019-08-14 Last updated: 2019-08-14Bibliographically approved
Fuoco, T. & Finne Wistrand, A. (2019). Enhancing the Properties of Poly(epsilon-caprolactone) by Simple and Effective Random Copolymerization of epsilon-Caprolactone with p-Dioxanone. Biomacromolecules, 20(8), 3171-3180
Open this publication in new window or tab >>Enhancing the Properties of Poly(epsilon-caprolactone) by Simple and Effective Random Copolymerization of epsilon-Caprolactone with p-Dioxanone
2019 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 20, no 8, p. 3171-3180Article in journal (Refereed) Published
Abstract [en]

We have developed a straightforward strategy to obtain semicrystalline and random copolymers of epsilon-caprolactone (CL) and p-dioxanone (DX) with thermal stabilities similar to poly(epsilon-caprolactone), PCL, but with a faster- hydrolytic degradation rate-CL/DX-copolymers-are promising inks when printing scaffolds aimed for tissue engineering. Such copolymers behave similar to PCL and resorb faster. The copolymers were synthesized by bulk ring-opening copolymerization, achieving a high yield; a molecular weight, M-n, of 57-176 kg mol(-1); and an inherent viscosity of 1.7-1.9 dL g(-1). The copolymer microstructure consisted of long CL blocks that are separated by isolated DX units. The block length and the melting point were a linear function of the DX content. The copolymers crystallize as an orthorhombic lattice that is typical for PCL, and they formed more elastic, softer, and less hydrophobic films with faster degradation rates than PCL. Relatively high thermal degradation temperatures (above 250 C), similar to PCL, were estimated by thermogravimetric analysis, and copolymer filaments for three-dimensional printing and scaffolds were produced without thermal degradation.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2019
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-257817 (URN)10.1021/acs.biomac.9b00745 (DOI)000480826700027 ()31268691 (PubMedID)2-s2.0-85071333322 (Scopus ID)
Note

QC 20190906

Available from: 2019-09-06 Created: 2019-09-06 Last updated: 2019-09-06Bibliographically approved
Fuoco, T., Mathisen, T. & Finne Wistrand, A. (2019). Minimizing the time gap between service lifetime and complete resorption of degradable melt-spun multifilament fibers. Polymer degradation and stability, 163, 43-51
Open this publication in new window or tab >>Minimizing the time gap between service lifetime and complete resorption of degradable melt-spun multifilament fibers
2019 (English)In: Polymer degradation and stability, ISSN 0141-3910, E-ISSN 1873-2321, Vol. 163, p. 43-51Article in journal (Refereed) Published
Abstract [en]

We have succeeded to modulated the degradation rate of poly(L-lactide) (PLLA) melt-spun multifilament fibers to extend the service lifetime and increase the resorption rate by using random copolymers of L-lactide and trimethylene carbonate (TMC). The presence of TMC units enabled an overall longer service lifetime but faster degradation kinetics than PLLA. By increasing the amount of TMC up to 18 mol%, multifilament fibers characterized by a homogenous degradation profile could be achieved. Such composition allowed, once the mechanical integrity was lost, a much longer retention of mechanical integrity and a faster rate of mass loss than samples containing less TMC. The degradation profile of multifilament fibers consisting of (co)polymers containing 0, 5, 10 and 18 mol% of TMC has been identified during 45 weeks in vitro hydrolysis following the molecular weight decrease, mass loss and changes in microstructure, crystallinity and mechanical properties. The fibers degraded by a two-step, autocatalyzed bulk hydrolysis mechanism. A high rate of molecular weight decrease and negligible mass loss, with a consequent drop of the mechanical properties, was observed in the early stage of degradation for fibers having TMC content up to 10 mol%. The later stage of degradation was, for these samples, characterized by a slight increase in the mass loss and a negligible molecular weight decrease. Fibers prepared with the 18 mol% TMC copolymer showed instead a more homogenous molecular weight decrease ensuring mechanical integrity for longer time and faster mass loss during the later stage of degradation.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Bulk degradation, Degradable copolymers, Melt-spun multifilament fibers, Poly(L-lactide-co-trimethylene carbonate), Service lifetime
National Category
Polymer Technologies
Identifiers
urn:nbn:se:kth:diva-246427 (URN)10.1016/j.polymdegradstab.2019.02.026 (DOI)000468250100006 ()2-s2.0-85062444715 (Scopus ID)
Note

QC 20190329

Available from: 2019-03-29 Created: 2019-03-29 Last updated: 2019-10-24Bibliographically approved
Fuoco, T., Mathisen, T. & Finne Wistrand, A. (2019). Poly(L-lactide) and Poly(L-lactide-co-trimethylene carbonate) Melt-Spun Fibers: Structure-Processing-Properties Relationship. Biomacromolecules, 20(3), 1346-1361
Open this publication in new window or tab >>Poly(L-lactide) and Poly(L-lactide-co-trimethylene carbonate) Melt-Spun Fibers: Structure-Processing-Properties Relationship
2019 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 20, no 3, p. 1346-1361Article in journal (Refereed) Published
Abstract [en]

l-Lactide/trimethylene carbonate copolymers have been produced as multifilament fibers by high-speed melt-spinning. The relationship existing between the composition, processing parameters and physical properties of the fibers has been disclosed by analyzing how the industrial process induced changes at the macromolecular level, i.e., the chain microstructure and crystallinity development. A poly(l-lactide) and three copolymers having trimethylene carbonate contents of 5, 10 and 18 mol % were synthesized with high molecular weight (M n ) up to 377 kDa and narrow dispersity. Their microstructure, crystallinity and thermal properties were dictated by the composition. The spinnability was then assessed for all the as-polymerized materials: four melt-spun multifilament fibers with increasing linear density were collected for each (co)polymer at a fixed take-up speed of 1800 m min -1 varying the mass throughput during the extrusion. A linear correlation resulted between the as-spun fiber properties and the linear density. The as-spun fibers could be further oriented, developing more crystallinity and improving their tensile properties by a second stage of hot-drawing. This ability was dependent on the composition and crystallinity achieved during the melt-spinning and the parameters selected for the hot-drawing, such as temperature, draw ratio and input speed. The crystalline structure evolved to a more stable form, and the degree of crystallinity increased from 0-52% to 25-66%. Values of tensile strength and Young's modulus up to 0.32-0.61 GPa and 4.9-8.4 GPa were respectively achieved.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2019
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-248094 (URN)10.1021/acs.biomac.8b01739 (DOI)000461270500022 ()30665299 (PubMedID)2-s2.0-85061266532 (Scopus ID)
Note

QC 20190429

Available from: 2019-04-29 Created: 2019-04-29 Last updated: 2019-05-20Bibliographically approved
Ojansivu, M., Rashad, A., Ahlinder, A., Massera, J., Mishra, A., Syverud, K., . . . Mustafa, K. (2019). Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells. Biofabrication, 11(3), Article ID 035010.
Open this publication in new window or tab >>Wood-based nanocellulose and bioactive glass modified gelatin-alginate bioinks for 3D bioprinting of bone cells
Show others...
2019 (English)In: Biofabrication, ISSN 1758-5082, E-ISSN 1758-5090, Vol. 11, no 3, article id 035010Article in journal (Refereed) Published
Abstract [en]

A challenge in the extrusion-based bioprinting is to find a bioink with optimal biological and physicochemical properties. The aim of this study was to evaluate the influence of wood-based cellulose nanofibrils (CNF) and bioactive glass (BaG) on the rheological properties of gelatin-alginate bioinks and the initial responses ofbone cells embedded in these inks. CNF modulated the flow behavior of the hydrogels, thus improving their printability. Chemical characterization by SEM-EDX and ion release analysis confirmed the reactivity of the BaG in the hydrogels. The cytocompatibility of the hydrogels was shown to be good, as evidenced by the viability of human osteoblast-like cells (Saos-2) in cast hydrogels. For bioprinting, 4-layer structures were printed from cell-containing gels and crosslinked with CaCl2. Viability, proliferation and alkaline phosphatase activity (ALP) were monitored over 14 d. In the BaG-free gels, Saos-2 cells remained viable, but in the presence of BaG the viability and proliferation decreased in correlation with the increased viscosity. Still, there was a constant increase in the ALP activity in all the hydrogels. Further bioprinting experiments were conducted using human bone marrow-derived mesenchymal stem cells (hBMSCs), a clinically relevant cell type. Interestingly, hBMSCs tolerated the printing process better than Saos-2 cells and the ALP indicated BaG-stimulated early osteogenic commitment. The addition of CNF and BaG to gelatin-alginate bioinks holds great potential for bone tissue engineering applications.

Place, publisher, year, edition, pages
Institute of Physics Publishing (IOPP), 2019
Keywords
bioprinting, viscosity, Saos-2, mesenchymal stem cell, bone tissue engineering, bioink, cellulose nanofibril
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-251692 (URN)10.1088/1758-5090/ab0692 (DOI)000466273600004 ()30754034 (PubMedID)2-s2.0-85063390553 (Scopus ID)
Note

QC 20190520

Available from: 2019-05-20 Created: 2019-05-20 Last updated: 2019-07-29Bibliographically approved
Ramani-Mohan, R.-K. -., Schwedhelm, I., Wistrand, A. F., Krug, M., Schwarz, T., Jakob, F., . . . Hansmann, J. (2018). Deformation strain is the main physical driver for skeletal precursors to undergo osteogenesis in earlier stages of osteogenic cell maturation. Journal of Tissue Engineering and Regenerative Medicine, 12(3), e1474-e1479
Open this publication in new window or tab >>Deformation strain is the main physical driver for skeletal precursors to undergo osteogenesis in earlier stages of osteogenic cell maturation
Show others...
2018 (English)In: Journal of Tissue Engineering and Regenerative Medicine, ISSN 1932-6254, E-ISSN 1932-7005, Vol. 12, no 3, p. e1474-e1479Article in journal (Refereed) Published
Abstract [en]

Mesenchymal stem cells play a major role during bone remodelling and are thus of high interest for tissue engineering and regenerative medicine applications. Mechanical stimuli, that is, deformation strain and interstitial fluid-flow-induced shear stress, promote osteogenic lineage commitment. However, the predominant physical stimulus that drives early osteogenic cell maturation is not clearly identified. The evaluation of each stimulus is challenging, as deformation and fluid-flow-induced shear stress interdepend. In this study, we developed a bioreactor that was used to culture mesenchymal stem cells harbouring a strain-responsive AP-1 luciferase reporter construct, on porous scaffolds. In addition to the reporter, mineralization and vitality of the cells was investigated by alizarin red staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. Quantification of the expression of genes associated to bone regeneration and bone remodelling was used to confirm alizarin red measurements. Controlled perfusion and deformation of the 3-dimensional scaffold facilitated the alteration of the expression of osteogenic markers, luciferase activity, and calcification. To isolate the specific impact of scaffold deformation, a computational model was developed to derive a perfusion flow profile that results in dynamic shear stress conditions present in periodically loaded scaffolds. In comparison to actually deformed scaffolds, a lower expression of all measured readout parameters indicated that deformation strain is the predominant stimulus for skeletal precursors to undergo osteogenesis in earlier stages of osteogenic cell maturation. 

Place, publisher, year, edition, pages
John Wiley and Sons Ltd, 2018
Keywords
bioreactor, computational fluid dynamics, mechanical strain, mechanosensitive reporter gene constructs, mesenchymal stem cells, osteogenesis, 3 (4, 5 dimethyl 2 thiazolyl) 2, 5 diphenyltetrazolium bromide, alizarin red s, alkaline phosphatase, bone morphogenetic protein 2, collagen type 1, osteocalcin, osteonectin, osteopontin, transcription factor AP 1, transcription factor RUNX2, Article, bone deformation, bone development, bone mineralization, bone regeneration, bone remodeling, bone stress, cell culture, cell differentiation, cell lineage, cell maturation, controlled study, enzyme activity, gene expression, human, human cell, luciferase assay, mathematical model, mesenchymal stem cell, osteoblast, porosity, priority journal, quantitative analysis, shear stress, stem cell, stress, three dimensional imaging
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-227413 (URN)10.1002/term.2565 (DOI)000427137100016 ()2-s2.0-85043365425 (Scopus ID)
Note

Export Date: 9 May 2018; Article; Correspondence Address: Hansmann, J.; Translational Center Würzburg “Regenerative Therapies for Oncology and Musculosceletal Diseases”, Branch of Fraunhofer Institute for Interfacial Engineering and Biotechnology IGBGermany; email: jan.hansmann@uni-wuerzburg.de; Funding details: 242175, FP7, Seventh Framework Programme; Funding details: 13N12971— ETface, MOHESR, Ministry of Higher Education and Scientific Research; Funding text: Our work was funded by the German Federal Ministry of Education and Research, program NanoMatFutur, grant agreement 13N12971— ETface, and the European Union's Seventh Framework Program, grant agreement 242175—Vascubone. Furthermore, we thank Moustapha Kassem for providing the TERT cells. The authors woud also like to thank Ernst‐Ulrich Berndt for performing µ‐CT imaging on the scaffold. QC 20180530

Available from: 2018-05-30 Created: 2018-05-30 Last updated: 2018-10-19Bibliographically approved
Sharma, S., Sapkota, D., Xue, Y., Rajthala, S., Yassin, M. A., Wistrand, A. F. & Mustafa, K. (2018). Delivery of VEGFA in bone marrow stromal cells seeded in copolymer scaffold enhances angiogenesis, but is inadequate for osteogenesis as compared with the dual delivery of VEGFA and BMP2 in a subcutaneous mouse model. Stem Cell Research & Therapy, 9, Article ID 23.
Open this publication in new window or tab >>Delivery of VEGFA in bone marrow stromal cells seeded in copolymer scaffold enhances angiogenesis, but is inadequate for osteogenesis as compared with the dual delivery of VEGFA and BMP2 in a subcutaneous mouse model
Show others...
2018 (English)In: Stem Cell Research & Therapy, E-ISSN 1757-6512, Vol. 9, article id 23Article in journal (Refereed) Published
Abstract [en]

Background: In bone tissue engineering (BTE), extensive research into vascular endothelial growth factor A (VEGFA)-mediated angiogenesis has yielded inconsistent results. The aim of this study was to investigate the influence on angio-and osteogenesis of adenoviral-mediated delivery of VEGFA alone or in combination with bone morphogenetic protein 2 (BMP2) in bone marrow stromal cells (BMSC) seeded onto a recently developed poly(LLA-co-CL) scaffold. Methods: Human BMSC were engineered to express VEGFA alone or in combination with BMP2 and seeded onto poly(LLA-co-CL) scaffolds. Changes in angiogenic and osteogenic gene and protein levels were examined by quantitative reverse-transcription polymerase chain reaction (RT-PCR), PCR array, and alkaline phosphatase assay. An in vivo subcutaneous mouse model was used to investigate the effect on angio-and osteogenesis of VEGFA alone or in combination with BMP2, using microcomputed tomography (mu CT), histology, immunohistochemistry, and immunofluorescence. Results: Combined delivery of a lower ratio (1: 3) of VEGFA and BMP2 (ad-BMP2 + VEGFA) led to upregulation of osteogenic and angiogenic genes in vitro at 3 and 14 days, compared with mono-delivery of VEGFA (ad-VEGFA) and other controls. In vivo, in a subcutaneous mouse model, both ad-VEGFA and ad-BMP2 + VEGFA scaffold explants exhibited increased angiogenesis at 2 weeks. Enhanced angiogenesis was largely related to the recruitment and differentiation of mouse progenitor cells to the endothelial lineage and, to a lesser extent, to endothelial differentiation of the implanted BMSC. mu CT and histological analyses revealed enhanced de novo bone formation only in the ad-BMP2 + VEGFA group, corresponding at the molecular level to the upregulation of genes related to osteogenesis, such as ALPL, RUNX2, and SPP1. Conclusions: Although BMSC expressing VEGFA alone or in combination with BMP2 significantly induced angiogenesis, VEGFA alone failed to demonstrate osteogenic activity both in vitro and in vivo. These results not only call into question the use of VEGFA alone in bone regeneration, but also highlight the importance in BTE of appropriately formulated combined delivery of VEGFA and BMP2.

Place, publisher, year, edition, pages
BioMed Central, 2018
Keywords
Bone regeneration, Scaffold, Mesenchymal stem cell, BMP2 and VEGFA, Angiogenesis, Gene delivery
National Category
Cell Biology
Identifiers
urn:nbn:se:kth:diva-223279 (URN)10.1186/s13287-018-0778-4 (DOI)000423686300006 ()29386057 (PubMedID)2-s2.0-85041627712 (Scopus ID)
Funder
EU, FP7, Seventh Framework Programme, 242175
Note

QC 20180219

Available from: 2018-02-19 Created: 2018-02-19 Last updated: 2018-02-19Bibliographically approved
Ahlinder, A., Fuoco, T. & Finne Wistrand, A. (2018). Medical grade polylactide, copolyesters and polydioxanone: Rheological properties and melt stability. Polymer testing, 72, 214-222
Open this publication in new window or tab >>Medical grade polylactide, copolyesters and polydioxanone: Rheological properties and melt stability
2018 (English)In: Polymer testing, ISSN 0142-9418, E-ISSN 1873-2348, Vol. 72, p. 214-222Article in journal (Refereed) Published
Abstract [en]

Rheological measurements have shown that lactide-based copolymers with L-lactide content between 50 and 100 mol% with varying comonomers, as well as polydioxanone (PDX), can be used in additive manufacturing analogously to poly(L-lactide) (PLLA) if their melt behaviour are balanced. The results indicate that copolymers can be melt processed if the temperature is adjusted according to the melting point, and parameters such as the speed are tuned to conteract the elastic response. Small amplitude oscillatory shear (SAOS) rheology, thermal and chemical characterisation allowed us to map the combined effect of temperature and frequency on the behaviour of six degradable polymers and their melt stability. Values of complex viscosity and Tan delta obtained through nine time sweeps by varying temperature and frequency showed that the molecular structure and the number of methylene units influenced the results, copolymers of L-lactide with D-Lactide (PDLLA) or glycolide (PLGA) had an increased elastic response, while copolymers with trimethylene carbonate (PLATMC) or epsilon-caprolactone (PCLA) had a more viscous behaviour than PLLA, with respect to their relative melting points. PDLLA and PLGA require an increased temperature or lower speed when processed, while PLATMC and PCLA can be used at a lower temperature and/or higher speed than PLLA. PDX showed an increased viscosity compared to PLLA but a similar melt behaviour. Negligible chain degradation were observed, apart from PLGA.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD, 2018
Keywords
Degradable polymers, Melt rheology, Polyesters, Melt stability and polylactide
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-241208 (URN)10.1016/j.polymertesting.2018.10.007 (DOI)000454464600025 ()2-s2.0-85055196906 (Scopus ID)
Note

QC 20190118

Available from: 2019-01-18 Created: 2019-01-18 Last updated: 2019-04-08Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-1922-128X

Search in DiVA

Show all publications