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Publications (10 of 29) Show all publications
Chen, C., Pettersson, T., Illergård, J., Ek, M. & Wågberg, L. (2019). Influence of Cellulose Charge on Bacteria Adhesion and Viability to PVAm/CNF/PVAm-Modified Cellulose Model Surfaces. Biomacromolecules
Open this publication in new window or tab >>Influence of Cellulose Charge on Bacteria Adhesion and Viability to PVAm/CNF/PVAm-Modified Cellulose Model Surfaces
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2019 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602Article in journal (Refereed) Published
Abstract [en]

A contact-active antibacterial approach based on the physical adsorption of a cationic polyelectrolyte onto the surface of a cellulose material is today regarded as an environment-friendly way of creating antibacterial surfaces and materials. In this approach, the electrostatic charge of the treated surfaces is considered to be an important factor for the level of bacteria adsorption and deactivation/killing of the bacteria. In order to clarify the influence of surface charge density of the cellulose on bacteria adsorption as well as on their viability, bacteria were adsorbed onto cellulose model surfaces, which were modified by physically adsorbed cationic polyelectrolytes to create surfaces with different positive charge densities. The surface charge was altered by the layer-by-layer (LbL) assembly of cationic polyvinylamine (PVAm)/anionic cellulose nanofibril/PVAm onto the initially differently charged cellulose model surfaces. After exposing the LbL-treated surfaces to Escherichia coli in aqueous media, a positive correlation was found between the adsorption of bacteria as well as the ratio of nonviable/viable bacteria and the surface charge of the LbL-modified cellulose. By careful colloidal probe atomic force microscopy measurements, it was estimated, due to the difference in surface charges, that interaction forces at least 50 nN between the treated surfaces and a bacterium could be achieved for the surfaces with the highest surface charge, and it is suggested that these considerable interaction forces are sufficient to disrupt the bacterial cell wall and hence kill the bacteria.

National Category
Biochemistry and Molecular Biology
Research subject
Fibre and Polymer Science
Identifiers
urn:nbn:se:kth:diva-249635 (URN)10.1021/acs.biomac.9b00297 (DOI)000468120800025 ()2-s2.0-85065660991 (Scopus ID)
Note

QC 20190611

Available from: 2019-04-15 Created: 2019-04-15 Last updated: 2019-06-11Bibliographically approved
Ottenhall, A., Henschen, J., Illergård, J. & Ek, M. (2018). Cellulose-based water purification using paper filters modified with polyelectrolyte multilayers to remove bacteria from water through electrostatic interactions. Environmental Science: Water Research & Technology
Open this publication in new window or tab >>Cellulose-based water purification using paper filters modified with polyelectrolyte multilayers to remove bacteria from water through electrostatic interactions
2018 (English)In: Environmental Science: Water Research & Technology, ISSN 2053-1400Article in journal (Refereed) Published
Abstract [en]

Filtration is a common way to obtain pure drinking water by removing particles and microorganisms based on size exclusion. Cellulose-based filters are affordable and biobased option for the removal of particles but bacteria are usually too small to be removed by size exclusion alone. In this article, the surfaces of cellulose fibres in two types of commercial paper filters have been given a positive net charge to trap bacteria through electrostatic interactions without releasing any biocides. The fibres were modified with the cationic polyelectrolyte polyvinylamine polymer in single layers (1 L) or in multilayers together with the anionic polyelectrolyte polyacrylic acid (3 L or 5 L) using a water-based process at room temperature. Filtration tests show that all filters, using both types of filter papers and a number of layers, can physically remove more than 99.9% of E. coli from water and that the 3 L modified filters can remove more than 97% of cultivatable bacteria from natural water samples. The bacterial reduction increased with increasing number of filter sheets used for the filtration and the majority of the bacteria were trapped in the top sheets of the filter. The results show the potential for creating water purification filters from bio-based everyday consumable products with a simple modification process. The filters could be used in the future for point-of-use water purification that may be able to save lives without releasing bactericides.

Keywords
Cellulose, water treatment, paper filter, membrane, filter, antibacterial, remove bacteria, layer-by-layer, Cellulosa, pappersfilter, vattenrening, membran, lager-på-lager, antibakteriell
National Category
Water Treatment
Research subject
Fibre and Polymer Science
Identifiers
urn:nbn:se:kth:diva-238838 (URN)10.1039/C8EW00514A (DOI)000451072500017 ()2-s2.0-85057384523 (Scopus ID)
Funder
ÅForsk (Ångpanneföreningen's Foundation for Research and Development), 17-391
Note

QC 20181112

Available from: 2018-11-12 Created: 2018-11-12 Last updated: 2019-08-27Bibliographically approved
Chen, C., Illergård, J., Wågberg, L. & Ek, M. (2017). Effect of cationic polyelectrolytes in contact-active antibacterial layer-by-layer functionalization. Paper presented at 14th European Workshop on Lignocellulosics and Pulp (EWLP), JUN, 2016, Autrans, FRANCE. Holzforschung, 71(7-8), 649-658
Open this publication in new window or tab >>Effect of cationic polyelectrolytes in contact-active antibacterial layer-by-layer functionalization
2017 (English)In: Holzforschung, ISSN 0018-3830, E-ISSN 1437-434X, Vol. 71, no 7-8, p. 649-658Article in journal (Refereed) Published
Abstract [en]

Contact-active surfaces have been created by means of the layer-by-layer (LbL) modification technique, which is based on previous observations that cellulose fibers treated with polyelectrolyte multilayers with polyvinylamine (PVAm) are perfectly protected against bacteria. Several different cationic polyelectrolytes were applied, including PVAm, two different poly(diallyl dimethyl ammonium chloride) polymers and two different poly(allylamine hydrochloride) polymers. The polyelectrolytes were self-organized in one or three layers on cellulosic fibers in combination with polyacrylic acid by the LbL method, and their antibacterial activities were evaluated. The modified cellulose fibers showed remarkable bacterial removal activities and inhibited bacterial growth. It was shown that the interaction between bacteria and modified fibers is not merely a charge interaction because a certain degree of bacterial cell deformation was observed on the modified fiber surfaces. Charge properties of the modified fibers were determined based on polyelectrolyte titration and zeta potential measurements, and a correlation between high charge density and antibacterial efficiency was observed for the PVAm and PDADMAC samples. It was demonstrated that it is possible to achieve antibacterial effects by the surface modification of cellulosic fibers via the LbL technique with different cationic polyelectrolytes.

Place, publisher, year, edition, pages
WALTER DE GRUYTER GMBH, 2017
Keywords
antibacterial, cellulosic fiber, fiber modification, layer by layer, nonleaching, polyelectrolyte
National Category
Polymer Technologies
Identifiers
urn:nbn:se:kth:diva-211388 (URN)10.1515/hf-2016-0184 (DOI)000404721500016 ()2-s2.0-85023188979 (Scopus ID)
Conference
14th European Workshop on Lignocellulosics and Pulp (EWLP), JUN, 2016, Autrans, FRANCE
Note

QC 20170808

Available from: 2017-08-08 Created: 2017-08-08 Last updated: 2017-08-08Bibliographically approved
Chen, C., Illergård, J., Wågberg, L. & Ek, M. (2017). Evaluation of Antibacterial functionalizations of CNF/PVAm multilayer modified cellulose fibre and surface studies on silica model surface. Paper presented at 253rd National Meeting of the American-Chemical-Society (ACS) on Advanced Materials, Technologies, Systems, and Processes, APR 02-06, 2017, San Francisco, CA. Abstract of Papers of the American Chemical Society, 253
Open this publication in new window or tab >>Evaluation of Antibacterial functionalizations of CNF/PVAm multilayer modified cellulose fibre and surface studies on silica model surface
2017 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 253Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2017
National Category
Organic Chemistry
Identifiers
urn:nbn:se:kth:diva-242588 (URN)000430568503483 ()
Conference
253rd National Meeting of the American-Chemical-Society (ACS) on Advanced Materials, Technologies, Systems, and Processes, APR 02-06, 2017, San Francisco, CA
Note

QC 20190226

Available from: 2019-02-26 Created: 2019-02-26 Last updated: 2019-02-26Bibliographically approved
Ottenhall, A., Illergård, J. & Ek, M. (2017). Water Purification Using Functionalized Cellulosic Fibers with Nonleaching Bacteria Adsorbing Properties. Environmental Science and Technology, 51, 7616-7623
Open this publication in new window or tab >>Water Purification Using Functionalized Cellulosic Fibers with Nonleaching Bacteria Adsorbing Properties
2017 (English)In: Environmental Science and Technology, ISSN 0013-936X, E-ISSN 1520-5851, Vol. 51, p. 7616-7623Article in journal (Refereed) Published
Abstract [en]

Portable purification systems are easy ways to obtain clean drinking water when there is no large-scale water treatment available. In this study, the potential to purify water using bacteria adsorbing cellulosic fibers, functionalized with polyelectrolytes according to the layer-by-layer method, is investigated. The adsorbed polyelectrolytes create a positive charge on the fiber surface that physically attracts and bonds with bacteria. Three types of cellulosic materials have been modified and tested for the bacterial removal capacity in water. The time, material-water ratio and bacterial concentration dependence, as well as the bacterial removal capacity in water from natural sources, have been evaluated. Freely dispersed bacteria adsorbing cellulosic fibers can remove greater than 99.9% of Escherichia coli from nonturbid water, with the most notable reduction occurring within the first hour. A filtering approach using modified cellulosic fibers is desirable for purification of natural water. An initial filtration test showed that polyelectrolyte multilayer modified cellulosic fibers can remove greater than 99% of bacteria from natural water. The bacteria adsorbing cellulosic fibers do not leach any biocides, and it is an environmentally sustainable and cheap option for disposable water purification devices.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2017
Keywords
Water treatment, membrane, filter, cellulose, cationic, contact-active, antibacterial, paper, Point-of-Use, polyelectrolyte, layer-by-layer, Vattenrening, pappersfilter, filter, cellulosa, kontaktaktiv, antibakteriell, positiv laddning, lager-på-lager, polyelektrolytmodifiering
National Category
Water Treatment
Research subject
Fibre and Polymer Science
Identifiers
urn:nbn:se:kth:diva-238837 (URN)10.1021/acs.est.7b01153 (DOI)
Note

QC 20181112

Available from: 2018-11-12 Created: 2018-11-12 Last updated: 2018-11-12Bibliographically approved
Ottenhall, A., Ek, M. & Illergård, J. (2017). Water Purification Using Functionalized Cellulosic Fibers with Nonleaching Bacteria Adsorbing Properties. Environmental Science and Technology, 13, 7616-7623
Open this publication in new window or tab >>Water Purification Using Functionalized Cellulosic Fibers with Nonleaching Bacteria Adsorbing Properties
2017 (English)In: Environmental Science and Technology, ISSN 0013-936X, E-ISSN 1520-5851, Vol. 13, p. 7616-7623Article in journal (Refereed) Published
Abstract [en]

 Portable purifi cation systems are easy ways to obtain clean drinking water when there is no large-scale water treatment available. In this study, the potential to purify water using bacteria adsorbing cellulosic fi bers, functionalized with polyelectrolytes according to the layer-by-layer method, is investigated. The adsorbed polyelectrolytes create a positive charge on the fi ber surface that physically attracts and bonds with bacteria. Three types of cellulosic materials have been modifi ed and tested for the bacterial removal capacity in water. The time, material-water ratio and bacterial concentration dependence, as well as the bacterial removal capacity in water from natural sources, have been evaluated. Freely dispersed bacteria adsorbing cellulosic fi bers can remove greater than 99.9% of Escherichia coli  from nonturbid water, with the most notable reduction occurring within the fi rst hour. A fi ltering approach using modifi ed cellulosic fi bers is desirable for purifi cation of natural water. An initial fi ltration test showed that polyelectrolyte multilayer modifi ed cellulosic fi bers can remove greater than 99% of bacteria from natural water. The bacteria adsorbing cellulosic fi bers do not leach any biocides, and it is an environmentally sustainable and cheap option for disposable water purification devices.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2017
Keywords
cellulose, water treatment, water purification, layer by layer, multilayer adsorption, bacteria removing, Vattenrening, cellulosa, pappersmassa, multilager, bakterieadsorption
National Category
Paper, Pulp and Fiber Technology
Research subject
Fibre and Polymer Science
Identifiers
urn:nbn:se:kth:diva-218668 (URN)10.1021/acs.est.7b01153 (DOI)000405056200035 ()2-s2.0-85024408693 (Scopus ID)
Note

QC 20171204

Available from: 2017-11-30 Created: 2017-11-30 Last updated: 2018-11-12Bibliographically approved
Ek, M., Illergård, J., Chen, C. & Wågberg, L. (2016). Biointeractive fibers with antibacterial properties. Abstracts of Papers of the American Chemical Society, 251
Open this publication in new window or tab >>Biointeractive fibers with antibacterial properties
2016 (English)In: Abstracts of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 251Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2016
National Category
Organic Chemistry
Identifiers
urn:nbn:se:kth:diva-242673 (URN)000431903802541 ()
Note

QC 20190222

Available from: 2019-02-22 Created: 2019-02-22 Last updated: 2019-08-21Bibliographically approved
Henschen, J., Illergård, J., Larsson, P. A., Ek, M. & Wågberg, L. (2016). Contact-active antibacterial aerogels from cellulose nanofibrils. Colloids and Surfaces B: Biointerfaces, 146, 415-422
Open this publication in new window or tab >>Contact-active antibacterial aerogels from cellulose nanofibrils
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2016 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 146, p. 415-422Article in journal (Refereed) Published
Abstract [en]

The use of cellulose aerogels as antibacterial materials has been investigated by applying a contact-active layer-by-layer modification to the aerogel surface. Studying the adsorption of multilayers of polyvinylamine (PVAm) and polyacrylic acid to aerogels comprising crosslinked cellulose nanofibrils and monitoring the subsequent bacterial adhesion revealed that up to 26 mg PVAm g aerogel−1 was adsorbed without noticeably affecting the aerogel structure. The antibacterial effect was tested by measuring the reduction of viable bacteria in solution when the aerogels were present. The results show that >99.9% of the bacteria adhered to the surface of the aerogels. Microscopy further showed adherence of bacteria to the surfaces of the modified aerogels. These results indicate that it is possible to create materials with three-dimensional cellulose structures that adsorb bacteria with very high efficiency utilizing the high specific surface area of the aerogels in combination with their open structure.

Keywords
Antibacterial, Cellulose nanofibrils, Aerogel, Contact active, Polymer adsorption
National Category
Paper, Pulp and Fiber Technology
Research subject
Materials Science and Engineering
Identifiers
urn:nbn:se:kth:diva-185001 (URN)10.1016/j.colsurfb.2016.06.031 (DOI)000382269600046 ()2-s2.0-84977269377 (Scopus ID)
Funder
VINNOVA
Note

QC 20160825

Available from: 2016-04-07 Created: 2016-04-07 Last updated: 2019-06-02Bibliographically approved
Henschen, J., Illergård, J., Larsson, P., Ek, M. & Wågberg, L. (2015). Antibacterial surface modification of nanocellulosic materials. Abstract of Papers of the American Chemical Society, 249
Open this publication in new window or tab >>Antibacterial surface modification of nanocellulosic materials
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2015 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 249Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
AMER CHEMICAL SOC, 2015
National Category
Other Chemistry Topics
Identifiers
urn:nbn:se:kth:diva-243686 (URN)000411183301769 ()
Note

QC 20190305

Available from: 2019-03-05 Created: 2019-03-05 Last updated: 2019-03-05Bibliographically approved
Illergård, J., Wågberg, L. & Ek, M. (2015). Contact-active antibacterial multilayers on fibres: a step towards understanding the antibacterial mechanism by increasing the fibre charge. Cellulose (London), 22(3), 2023-2034
Open this publication in new window or tab >>Contact-active antibacterial multilayers on fibres: a step towards understanding the antibacterial mechanism by increasing the fibre charge
2015 (English)In: Cellulose (London), ISSN 0969-0239, E-ISSN 1572-882X, Vol. 22, no 3, p. 2023-2034Article in journal (Refereed) Published
Abstract [en]

Contact-active antibacterial materials with irreversibly attached antibacterial agents have been developed as an environmentally friendly alternative to traditional biocide treatments. Of particular interest are materials fabricated through the physical adsorption of charged polymers. This simple method allows for the use of water-based processes and materials originating from renewable sources, e.g., cellulosic fibres. Furthermore, by varying the process parameters, such as ionic strength, it is possible to tune the properties of the adsorbed polymer layer. However, the underlying antibacterial mechanism remains obscure, and this hinders the rational design of antibacterial multilayers. To gain further insight into the antibacterial mechanisms of physically adsorbed multilayers of polyvinylamine and polyacrylic acid, the surface charge of cellulose fibres was increased via radical oxidation. This oxidation increased the amount of polymer that was adsorbed and resulted in increased antibacterial efficacy against both Escherichia coli and Bacillus subtilis compared with polymer-modified unoxidised fibres. Electron microscopy analysis of the E. coli adhered to the fibres revealed that the multilayer treatment resulted in elongated bacteria with deformed cell walls. This work demonstrates the importance of electrostatic interaction to the antibacterial effect of polymer-modified fibres.

Keywords
Antibacterial materials, Contact-active, Antibacterial cellulose, Antibacterial mechanism, Polyvinylamine, Polyelectrolyte multilayers, Layer-by-layer, Polymer adsorption
National Category
Materials Engineering
Identifiers
urn:nbn:se:kth:diva-169246 (URN)10.1007/s10570-015-0629-8 (DOI)000354193000044 ()
Funder
VINNOVA
Note

QC 20150616

Available from: 2015-06-16 Created: 2015-06-12 Last updated: 2017-12-04Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-1812-7336

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