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Marabita, F., James, T., Karhu, A., Virtanen, H., Kettunen, K., Stenlund, H., . . . Kallioniemi, O. (2022). Article Multiomics and digital monitoring during lifestyle changes reveal independent dimensions of human biology and health. CELL SYSTEMS, 13(3), 241-255.e7
Open this publication in new window or tab >>Article Multiomics and digital monitoring during lifestyle changes reveal independent dimensions of human biology and health
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2022 (English)In: CELL SYSTEMS, ISSN 2405-4712, Vol. 13, no 3, p. 241-255.e7Article in journal (Refereed) Published
Abstract [en]

We explored opportunities for personalized and predictive health care by collecting serial clinical measurements, health surveys, genomics, proteomics, autoantibodies, metabolomics, and gut microbiome data from 96 individuals who participated in a data-driven health coaching program over a 16-month period with continuous digital monitoring of activity and sleep. We generated a resource of >20,000 biological samples from this study and a compendium of >53 million primary data points for 558,032 distinct features. Multiomics factor analysis revealed distinct and independent molecular factors linked to obesity, diabetes, liver function, cardiovascular disease, inflammation, immunity, exercise, diet, and hormonal effects. For example, ethinyl estradiol, a common oral contraceptive, produced characteristic molecular and physiological effects, including increased levels of inflammation and impact on thyroid, cortisol levels, and pulse, that were distinct from other sources of variability observed in our study. In total, this work illustrates the value of combining deep molecular and digital monitoring of human health. A record of this paper's transparent peer review process is included in the supplemental information.

Place, publisher, year, edition, pages
Elsevier BV, 2022
National Category
Bioinformatics and Computational Biology
Identifiers
urn:nbn:se:kth:diva-310765 (URN)10.1016/j.cels.2021.11.001 (DOI)000773114900006 ()34856119 (PubMedID)2-s2.0-85122964198 (Scopus ID)
Note

QC 20220407

Available from: 2022-04-07 Created: 2022-04-07 Last updated: 2025-02-07Bibliographically approved
Abdellah, T., Gummesson, A., Zhong, W., Koistinen, I. S., Lakshmikanth, T., Olsson, L. M., . . . Fagerberg, L. (2020). Integration of molecular profiles in a longitudinal wellness profiling cohort. Nature Communications, 11(1), Article ID 4487.
Open this publication in new window or tab >>Integration of molecular profiles in a longitudinal wellness profiling cohort
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2020 (English)In: Nature Communications, E-ISSN 2041-1723, Vol. 11, no 1, article id 4487Article in journal (Refereed) Published
Place, publisher, year, edition, pages
Nature Publishing Group, 2020
Keywords
General Physics and Astronomy, General Biochemistry, Genetics and Molecular Biology, General Chemistry
National Category
Bioinformatics and Computational Biology
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-303344 (URN)10.1038/s41467-020-18148-7 (DOI)000607103200020 ()32900998 (PubMedID)2-s2.0-85090387884 (Scopus ID)
Note

QC 20211110

Available from: 2021-10-12 Created: 2021-10-12 Last updated: 2025-02-07Bibliographically approved
Dezfouli, M., Bergström, S., Skattum, L., Abolhassani, H., Neiman, M., Torabi-Rahvar, M., . . . Hammarstrom, L. (2020). Newborn Screening for Presymptomatic Diagnosis of Complement and Phagocyte Deficiencies. Frontiers in Immunology, 11, Article ID 455.
Open this publication in new window or tab >>Newborn Screening for Presymptomatic Diagnosis of Complement and Phagocyte Deficiencies
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2020 (English)In: Frontiers in Immunology, E-ISSN 1664-3224, Vol. 11, article id 455Article in journal (Refereed) Published
Abstract [en]

The clinical outcomes of primary immunodeficiencies (PIDs) are greatly improved by accurate diagnosis early in life. However, it is not common to consider PIDs before the manifestation of severe clinical symptoms. Including PIDs in the nation-wide newborn screening programs will potentially improve survival and provide better disease management and preventive care in PID patients. This calls for the detection of disease biomarkers in blood and the use of dried blood spot samples, which is a part of routine newborn screening programs worldwide. Here, we developed a newborn screening method based on multiplex protein profiling for parallel diagnosis of 22 innate immunodeficiencies affecting the complement system and respiratory burst function in phagocytosis. The proposed method uses a small fraction of eluted blood from dried blood spots and is applicable for population-scale performance. The diagnosis method is validated through a retrospective screening of immunodeficient patient samples. This diagnostic approach can pave the way for an earlier, more comprehensive and accurate diagnosis of complement and phagocytic disorders, which ultimately lead to a healthy and active life for the PID patients.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA, 2020
Keywords
primary immunodeficiency, complement deficiencies, phagocytic disorders, presymptomatic diagnosis, newborn screening, dried blood spot, protein profiling
National Category
Medical Genetics and Genomics
Identifiers
urn:nbn:se:kth:diva-272811 (URN)10.3389/fimmu.2020.00455 (DOI)000525552000001 ()32256498 (PubMedID)2-s2.0-85082659138 (Scopus ID)
Funder
Science for Life Laboratory, SciLifeLab
Note

QC 20200428

Available from: 2020-04-28 Created: 2020-04-28 Last updated: 2025-02-10Bibliographically approved
Neiman, M., Hellström, C., Just, D., Mattsson, C., Fagerberg, L., Schuppe-Koistinen, I., . . . Nilsson, P. (2019). Individual and stable autoantibody repertoires in healthy individuals. Autoimmunity, 52(1), 1-11
Open this publication in new window or tab >>Individual and stable autoantibody repertoires in healthy individuals
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2019 (English)In: Autoimmunity, ISSN 0891-6934, E-ISSN 1607-842X, Vol. 52, no 1, p. 1-11Article in journal (Refereed) Published
Abstract [en]

In the era towards precision medicine, we here present the individual specific autoantibody signatures of 193 healthy individuals. The self-reactive IgG signatures are stable over time in a way that each individual profile is recognized in longitudinal sampling. The IgG autoantibody reactivity towards an antigen array comprising 335 protein fragments, representing 204 human proteins with potential relevance to autoimmune disorders, was measured in longitudinal plasma samples from 193 healthy individuals. This analysis resulted in unique autoantibody barcodes for each individual that were maintained over one year's time. The reactivity profiles, or signatures, are person specific in regards to the number of reactivities and antigen specificity. Two independent data sets were consistent in that each healthy individual displayed reactivity towards 0-16 antigens, with a median of six. Subsequently, four selected individuals were profiled on in-house produced high-density protein arrays containing 23,000 protein fragments representing 14,000 unique protein coding genes. Based on a unique, broad and deep longitudinal profiling of autoantibody reactivities, our results demonstrate a unique autoreactive profile in each analyzed healthy individual. The need and interest for broad-ranged and high-resolution molecular profiling of healthy individuals is rising. We have here generated and assessed an initial perspective on the global distribution of the self-reactive IgG repertoire in healthy individuals, by investigating 193 well-characterized healthy individuals.

Place, publisher, year, edition, pages
TAYLOR & FRANCIS LTD, 2019
Keywords
Autoantibody repertoire, autoantibody profile, protein array, affinity proteomics, precision medicine
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-249825 (URN)10.1080/08916934.2019.1581774 (DOI)000462921100001 ()30835561 (PubMedID)2-s2.0-85062520789 (Scopus ID)
Note

QC 20190423

Available from: 2019-04-23 Created: 2019-04-23 Last updated: 2024-03-15Bibliographically approved
Notarnicola, A., Hellström, C., Mattsson, C., Andersson, E., Idborg, H., Jemseby, E., . . . Lundberg, I. E. (2017). NEW AUTOIMMUNE TARGETS IN IDIOPATHIC INFLAMMATORY MYOPATHIES - AN ANTIGEN BEAD ARRAY APPROACH. Paper presented at Annual European Congress of Rheumatology, JUN 14-17, 2017, Madrid, SPAIN. Annals of the Rheumatic Diseases, 76, 626-626
Open this publication in new window or tab >>NEW AUTOIMMUNE TARGETS IN IDIOPATHIC INFLAMMATORY MYOPATHIES - AN ANTIGEN BEAD ARRAY APPROACH
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2017 (English)In: Annals of the Rheumatic Diseases, ISSN 0003-4967, E-ISSN 1468-2060, Vol. 76, p. 626-626Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
BMJ PUBLISHING GROUP, 2017
National Category
Basic Medicine
Identifiers
urn:nbn:se:kth:diva-217956 (URN)10.1136/annrheumdis-2017-eular.5863 (DOI)000413181401767 ()
Conference
Annual European Congress of Rheumatology, JUN 14-17, 2017, Madrid, SPAIN
Note

QC 20171121

Available from: 2017-11-21 Created: 2017-11-21 Last updated: 2024-03-15Bibliographically approved
Hamsten, C., Skattum, L., Truedsson, L., von Döbeln, U., Uhlén, M., Schwenk, J. M., . . . Neiman, M. (2015). Heat differentiated complement factor profiling. Journal of Proteomics, 126, 155-162
Open this publication in new window or tab >>Heat differentiated complement factor profiling
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2015 (English)In: Journal of Proteomics, ISSN 1874-3919, E-ISSN 1876-7737, Vol. 126, p. 155-162Article in journal (Refereed) Published
Abstract [en]

Complement components and their cascade of reactions are important defense mechanisms within both innate and adaptive immunity. Many complement deficient patients still remain undiagnosed because of a lack of high throughput screening tools. Aiming towards neonatal proteome screening for immunodeficiencies, we used a multiplex profiling approach with antibody bead arrays to measure 9 complement proteins in serum and dried blood spots. Several complement components have been described as heat sensitive, thus their heat-dependent detectability was investigated. Using sera from 16 patients with complement deficiencies and 23 controls, we confirmed that the proteins C1q, C2, C3, C6, C9 and factor H were positively affected by heating, thus the identification of deficient patients was improved when preheating samples. Measurements of C7, C8 and factor I were negatively affected by heating and non-heated samples should be used in analysis of these components. In addition, a proof of concept study demonstrated the feasibility of labeling eluates from dried blood spots to perform a subsequent correct classification of C2-deficiencies. Our study demonstrates the potential of using multiplexed single binder assays for screening of complement components that open possibilities to expand such analysis to other forms of deficiencies.

Keywords
Complement factors, Dried blood spots, Immunodeficiency, Multiplex immunoassay, Neonatal screening, Serum
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-170194 (URN)10.1016/j.jprot.2015.05.027 (DOI)000359884600014 ()26047714 (PubMedID)2-s2.0-84930942972 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20150630

Available from: 2015-06-30 Created: 2015-06-29 Last updated: 2024-03-18Bibliographically approved
Neiman, M. (2013). Bead based protein profiling in blood. (Doctoral dissertation). Stockholm: KTH Royal Institute of Technology
Open this publication in new window or tab >>Bead based protein profiling in blood
2013 (Swedish)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis is about protein profiling in blood-derived samples using suspension bead ar- rays built with protein affinity reagents, and the evaluation of binding characteristics and potential disease relation of such profiles.

A central aim of the presented work was to discover and verify disease associated protein profiles in blood-derived samples such as serum or plasma. This was based on immobiliz- ing antigens or antibodies on color-coded beads for a multiplexed analysis. This concept generally allow for a dual multiplexing because hundreds of samples can be screened for hundreds of proteins in a miniaturized and parallelized fashion. At first, protein antigens were used to study humoral immune responses in cattle suffering from a mycoplasma infec- tion (Paper I). Here, the most immunogenic of the applied antigens were identified based on reactivity profiles from the infected cattle, and were combined into an antigen cocktail to serve as a diagnostic assay in a standard ELISA set-up. Next, antibodies and their em- ployment in assays with directly labeled human samples was initiated. This procedure was applied in a study of kidney disorders where screening of plasma resulted in the discovery of a biomarker candidate, fibulin-1 (Paper II). In parallel to the disease related applica- tions, systematic evaluations of the protein profiles were conducted. Protein profiles from 2,300 antibodies were classified on the bases of binding properties in relation to sample heating and stringent washing (Paper III). With a particular focus on heat dependent de- tectability, a method was developed to visualize those proteins that were captured to the beads in an immunoassay by using Western blotting (Paper IV). In conclusion, this thesis presents examples of the possibilities of comparative plasma profiling enabled by protein bead arrays. 

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2013. p. 116
Series
Trita-BIO-Report, ISSN 1654-2312 ; 2013:4
Keywords
Affinity proteomics, protein array, suspension bead array, antigen, antibody, biomarker discovery, serology, selectivity, sensitivity, serum, plasma
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:kth:diva-117960 (URN)978-91-7501-629-0 (ISBN)
Public defence
2013-03-01, Gardaulan, Smittskyddsinstitutet, Nobels väg 18, Solna, 10:00 (English)
Opponent
Supervisors
Note

QC 20130208

Available from: 2013-02-08 Created: 2013-02-07 Last updated: 2022-06-24Bibliographically approved
Neiman, M., Fredolini, C., Johansson, H., Lehtiö, J., Nygren, P.-Å., Uhlén, M., . . . Schwenk, J. M. (2013). Selectivity analysis of single binder assays used in plasma protein profiling. Proteomics, 13(23-24), 3406-3410
Open this publication in new window or tab >>Selectivity analysis of single binder assays used in plasma protein profiling
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2013 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 13, no 23-24, p. 3406-3410Article in journal (Refereed) Published
Abstract [en]

The increasing availability of antibodies toward human proteins enables broad explorations of the proteomic landscape in cells, tissues, and body fluids. This includes assays with antibody suspension bead arrays that generate protein profiles of plasma samples by flow cytometer analysis. However, antibody selectivity is context dependent so it is necessary to corroborate on-target detection over off-target binding. To address this, we describe a concept to directly verify interactions from antibody-coupled beads by analysis of their eluates by Western blots and MS. We demonstrate selective antibody binding in complex samples with antibodies toward a set of chosen proteins with different abundance in plasma and serum, and illustrate the need to adjust sample and bead concentrations accordingly. The presented approach will serve as an important tool for resolving differential protein profiles from antibody arrays within plasma biomarker discoveries.

Keywords
Antibodies, Plasma profiling, Protein arrays, Suspension bead arrays, Verification
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-139859 (URN)10.1002/pmic.201300030 (DOI)000329994000005 ()24151238 (PubMedID)2-s2.0-84890306435 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceVinnovaKnut and Alice Wallenberg Foundation
Note

QC 20140122

Available from: 2014-01-22 Created: 2014-01-15 Last updated: 2024-03-15Bibliographically approved
Häggmark, A., Neiman, M., Drobin, K., Zwahlen, M., Uhlén, M., Nilsson, P. & Schwenk, J. M. (2011). Classification of protein profiles from antibody microarrays using heat and detergent treatment.. New Biotechnology, 29(5), 564-570
Open this publication in new window or tab >>Classification of protein profiles from antibody microarrays using heat and detergent treatment.
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2011 (English)In: New Biotechnology, ISSN 1871-6784, E-ISSN 1876-4347, Vol. 29, no 5, p. 564-570Article in journal (Refereed) Published
Abstract [en]

Antibody microarrays offer new opportunities for exploring the proteome and to identify biomarker candidates in human serum and plasma. Here, we have investigated the effect of heat and detergents on an antibody-based suspension bead array (SBA) assay using polyclonal antibodies and biotinylated plasma samples. With protein profiles from more than 2300 antibodies generated in 384-plex antibody SBAs, three major classes of heat and detergent susceptibility could be described. The results show that washing of the beads with SDS (rather than Tween) after target binding lowered intensity levels of basically all profiles and that about 50% of the profiles appeared to be lowered to a similar extent by heating of the sample. About 33% of the profiles appeared to be insensitive to heat treatment while another 17% showed a positive influence of heat to yield elevated profiles. The results suggest that the classification of antibodies is driven by the molecular properties of the antibody-antigen interaction and can generally not be predicted based on protein class or Western blot data. The experimental scheme presented here can be used to systematically categorize antibodies and thereby combine antibodies with similar properties into targeted arrays for analysis of plasma and serum.

National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-52450 (URN)10.1016/j.nbt.2011.10.005 (DOI)000305606500008 ()22023822 (PubMedID)2-s2.0-84862011672 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note
QC 20111216Available from: 2011-12-16 Created: 2011-12-16 Last updated: 2024-03-15Bibliographically approved
Neiman, M., Hedberg, J. J., Dönnes, P. R., Schuppe-Koistinen, I., Hanschke, S., Schindler, R., . . . Nilsson, P. (2011). Plasma Profiling Reveals Human Fibulin-1 as Candidate Marker for Renal Impairment. Journal of Proteome Research, 10(11), 4925-4934
Open this publication in new window or tab >>Plasma Profiling Reveals Human Fibulin-1 as Candidate Marker for Renal Impairment
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2011 (English)In: Journal of Proteome Research, ISSN 1535-3893, E-ISSN 1535-3907, Vol. 10, no 11, p. 4925-4934Article in journal (Refereed) Published
Abstract [en]

There is a need for reliable and sensitive biomarkers for renal impairments to detect early signs of kidney toxicity and to monitor progression of disease. Here, antibody suspension bead arrays were applied to profile plasma samples from patients with four types of kidney disorders: glomerulonephritis, diabetic nephropathy, obstructive uropathy, and analgesic abuse. In total, 200 clinical renal-associated cases and control plasma samples from different cohorts were profiled. Parallel plasma protein profiles were obtained using biotinylated and nonfractionated samples and a selected set of 94 proteins targeted by 129 antigen-purified polyclonal antibodies. Out of the analyzed target proteins, human fibulin-1 was detected at significantly higher levels in the glomerulonephritis patient group compared to the controls and with elevated levels in patient samples for all other renal disorders investigated. Two polyclonal antibodies and one monoclonal antibody directed toward separate, nonoverlapping epitopes showed the same trend in the discovery cohorts. A technical verification using Western blot analysis of selected patient plasma confirmed the trends toward higher abundance of the target protein in disease samples. Furthermore, a verification study was carried out in the context of glomerulonephritis using an independent case and control cohort, and this confirmed the results from the discovery cohort, suggesting that plasma levels of fibulin-1 could serve as a potential indicator to monitor kidney malfunction or kidney damage.

Keywords
affinity proteomics, plasma profiling, antibody microarray, kidney disorders, biomarker, fibulin-1, glomerulonephritis
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-51288 (URN)10.1021/pr200286c (DOI)000296414700004 ()21888404 (PubMedID)2-s2.0-80655139676 (Scopus ID)
Funder
Knut and Alice Wallenberg FoundationScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note
QC 20111212Available from: 2011-12-12 Created: 2011-12-12 Last updated: 2024-03-18Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-1855-703X

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