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Publications (10 of 13) Show all publications
Bi, R., Oinonen, P., Wang, Y. & Henriksson, G. (2016). A Method for Studying Effects on Lignin-Polysaccharide Networks during Biological Degradation and Technical Processes of Wood. BioResources, 11(1), 1307-1318
Open this publication in new window or tab >>A Method for Studying Effects on Lignin-Polysaccharide Networks during Biological Degradation and Technical Processes of Wood
2016 (English)In: BioResources, ISSN 1930-2126, E-ISSN 1930-2126, Vol. 11, no 1, p. 1307-1318Article in journal (Refereed) Published
Abstract [en]

Woody tissues consist primarily of a mixture of cellulose, hemicelluloses, and lignin. Covalent bonds between lignin and polysaccharides likely play a central role in determining the mechanical and physical properties of wood. Intact and defined lignin-polysaccharide networks have not been isolated in large quantities because of the recalcitrance of lignin, which demands harsh chemical treatments that alter its structure. This report presents a method for preparing large quantities of lignin-polysaccharide networks similar to those naturally present in wood based on the enzymatic oxidation of hemicellulose from Norway spruce. Fungal enzymes produced from various carbon sources were used to depolymerize these networks. The method was used for simulating "enzyme mining" - a concept in biorefineries, giving a possible explanation for its mechanisms.

Place, publisher, year, edition, pages
NORTH CAROLINA STATE UNIV, 2016
Keywords
Lignin carbohydrate complexes, Wood, Model system, Biorefinery, Biodegradation
National Category
Polymer Technologies
Identifiers
urn:nbn:se:kth:diva-182267 (URN)10.15376/biores.11.1.1307-1318 (DOI)000367732700103 ()2-s2.0-84981163489 (Scopus ID)
Funder
Knut and Alice Wallenberg Foundation
Note

QC 20160223

Available from: 2016-02-18 Created: 2016-02-18 Last updated: 2017-11-30Bibliographically approved
Bi, R., Huang, S. & Henriksson, G. (2016). ISOLATION OF EXCEEDINGLY LOW OXYGEN CONSUMING FUNGAL STRAINS ABLE TO UTILIZE LIGNIN AS CARBON SOURCE. Cellulose Chemistry and Technology, 50(7-8), 811-817
Open this publication in new window or tab >>ISOLATION OF EXCEEDINGLY LOW OXYGEN CONSUMING FUNGAL STRAINS ABLE TO UTILIZE LIGNIN AS CARBON SOURCE
2016 (English)In: Cellulose Chemistry and Technology, ISSN 0576-9787, Vol. 50, no 7-8, p. 811-817Article in journal (Refereed) Published
Abstract [en]

Lignin biodegradation is normally related to aerobic microorganisms, and it is often claimed that microbes do not metabolize lignin as a carbon source. In this work, several fungal strains were isolated from the sediment of a small stream located in a forest and tested on agar plates with lignin as the only carbon source. All identified strains were Ascomycetes, Penicillium spinulosum, Pseudeurotium bakeri and Galactomyces geotrichum. When cultivated in shaking flasks with lignosulphonate as a carbon source, the lignin was consumed, and cell free culture filtrates appeared to depolymerize lignosulphonate to some extent. It is suggested that the strains detected are part of a symbiotic community and live in a microbiological niche in which they are able to utilize lignin residues left from brown rot and humus having extremely low oxygen content.

Keywords
carbon source, extracellular enzymes, extremely low oxygen content, lignin biodegradation, soil microorganisms
National Category
Paper, Pulp and Fiber Technology
Identifiers
urn:nbn:se:kth:diva-202467 (URN)000392468700011 ()
Note

QC 20170302

Available from: 2017-03-02 Created: 2017-03-02 Last updated: 2017-11-29Bibliographically approved
Bi, R. (2016). Lignocellulose Degradation by Soil Micro-organisms. (Doctoral dissertation). Stockholm: KTH Royal Institute of Technology
Open this publication in new window or tab >>Lignocellulose Degradation by Soil Micro-organisms
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Lignocellulosic biomass is a sustainable resource with abundant reserves. Compared to petroleum ‐ based products, the biomass ‐ derived polymers and chemicals give better environmental profiles. A lot of research interest is focused on understanding the lignocellulose structures.

Lignin, among the three major wood components, represents most difficulty for microbial degradation because of its complex structure and because cross ‐ linking to hemicellulose makes wood such a compact structure. Nevertheless, wood is naturally degraded by wood ‐ degrading micro ‐ organisms and modified and partly degraded residual of lignin goes into soil. Therefore soil serves as a good environment in which to search for special lignin ‐ degraders. In this thesis, different types of lignin have been used as sole carbon sources to screen for lignin ‐ degrading soil micro ‐ organisms. Eleven aerobic and three anaerobic microbe strains have been isolated and identified as able to grow on lignin. The lignin degradation patterns of selected strains have been studied and these partly include an endwise cleavage of  β‐ O ‐ 4 bonds in lignin and is more complex than simple hydrolytic degradation.

As lignin exists in wood covalently bonded to hemicellulose, one isolated microbe strain, Phoma herbarum, has also been studied with regards to its ability to degrade covalent lignin polysaccharide networks (LCC). The results show that its culture filtrate can attack lignin ‐ polysaccharide networks in a manner different from that of the commercial enzyme product, Gammanase, possibly by selective cleavage of phenyl glucoside bonds. The effects on LCC of Phoma herbarum also enhance polymer extractability. Hot ‐ water extraction of a culture filtrate of Phoma herbarum ‐ treated fiberized spruce wood material gave an amount of extracted galactoglucomannan more than that given by the Gammanase ‐ treated material and non ‐ enzyme ‐ treated material.

Over millions of years of natural evolution, micro ‐ organisms on the one hand develop so that they can degrade all wood components to get energy for growth, while plants on the other hand also continuously develop to defend from microbial attack. Compared with lignin and cellulose, hemicelluloses as major components of plant cell walls, are much more easily degraded, but hemicelluloses differ from cellulose in that they are acetylated to different extents. The biological functions of acetylation are not completely understood, but it is suggested is that one function is to decrease the microbial degradability of cell walls. By cultivation of soil micro ‐ organisms using mannans acetylated to deffernent degrees as sole carbon source on agar plates, we were able to see significant trends where the resistance towards microbial degradation of glucomannan and galactomannan increased with increasing degree of acetylation. Possible mechanisms and the technological significance of this are discussed. Tailoring the degree of acetylation of polysaccharide materials might slow down the biodegradation, making it possible to design a material with a degradation rate suited to its application.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2016. p. 59
Series
TRITA-CHE-Report, ISSN 1654-1081 ; 2016:10
National Category
Polymer Technologies Biocatalysis and Enzyme Technology Microbiology Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-182336 (URN)978-91-7595-868-2 (ISBN)
Public defence
2016-03-18, Kollegiesalen, Brinellvägen 8, KTH, Stockholm, 10:00 (English)
Opponent
Supervisors
Funder
Knut and Alice Wallenberg Foundation
Note

QC 20160223

Available from: 2016-02-23 Created: 2016-02-18 Last updated: 2017-03-02Bibliographically approved
Bi, R., Spadiut, O., Lawoko, M., Brumer, H. & Henriksson, G. (2012). Isolation and identification of microorganisms from soil able to live on lignin as a carbon source and to produce enzymes which cleave beta-O-4 bond in a lignin model compound. Cellulose Chemistry and Technology, 46(3-4), 227-242
Open this publication in new window or tab >>Isolation and identification of microorganisms from soil able to live on lignin as a carbon source and to produce enzymes which cleave beta-O-4 bond in a lignin model compound
Show others...
2012 (English)In: Cellulose Chemistry and Technology, ISSN 0576-9787, Vol. 46, no 3-4, p. 227-242Article in journal (Refereed) Published
Abstract [en]

Several strains of fungi were isolated and identified from Scandinavian soil using agar plates with lignin as a carbon source. The strains grew significantly faster on this medium than on control plates without lignin. Different types of technical lignins were used, some of which contained trace amounts of sugars, even if the increased growth rate seemed not related to the sugar content. Some strains were cultivated in shaking flask cultures with lignin as a carbon source, with lignin apparently consumed by microbes - while accumulation of the microorganism biomass occurred. The cell-free filtrates of these cultures could reduce the apparent molecular weights of lignosulphonates, while the culture filtrate of one strain could cleave the beta-O-4 bond in a lignin model compound.

Keywords
lignin biodegradation, carbon source, soil microorganisms, extracellular enzymes, beta-O-4 bond
National Category
Chemical Engineering
Identifiers
urn:nbn:se:kth:diva-101134 (URN)000306779000010 ()2-s2.0-84864568077 (Scopus ID)
Note

QC 20120824

Available from: 2012-08-24 Created: 2012-08-23 Last updated: 2017-12-07Bibliographically approved
Bi, R., Spadiut, O., Brumer, H. & Henriksson, G. (2012). Isolation and identification of microorganisms from soil able to live on lignin as acarbon source and to produce enzymes which cleave the β-o-4 bond in a lignin model compound. Cellulose Chemistry and Technology, 46(3-4), 227-242
Open this publication in new window or tab >>Isolation and identification of microorganisms from soil able to live on lignin as acarbon source and to produce enzymes which cleave the β-o-4 bond in a lignin model compound
2012 (English)In: Cellulose Chemistry and Technology, ISSN 0576-9787, Vol. 46, no 3-4, p. 227-242Article in journal (Refereed) Published
Abstract [en]

Several strains of fungi were isolated and identified from Scandinavian soil using agar plates with lignin as a carbon source. The strains grew significantly faster on this medium than on control plates without lignin. Different types of technical lignins were used, some of which contained trace amounts of sugars, even if the increased growth rate seemed not related to the sugar content. Some strains were cultivated in shaking flask cultures with lignin as a carbon source, with lignin apparently consumed by microbes - while accumulation of the microorganism biomass occurred. The cell-free filtrates of these cultures could reduce the apparent molecular weights of lignosulphonates, while the culture filtrate of one strain could cleave the beta-O-4 bond in a lignin model compound.

Place, publisher, year, edition, pages
UBC, 2012
National Category
Chemical Process Engineering
Identifiers
urn:nbn:se:kth:diva-182239 (URN)
Note

QC 20160223

Available from: 2016-02-18 Created: 2016-02-18 Last updated: 2017-11-30Bibliographically approved
Bi, R. & Henriksson, G. (2012). Isolation and identification of soil microorganisms under anaerobic condition which is able to live on lignin as carbon source. Paper presented at 11th International Biorelated Polymer Symposium / 243rd National Spring Meeting of the American-Chemical-Society (ACS), MAR 25-29, 2012, San Diego, CA. Abstract of Papers of the American Chemical Society, 243
Open this publication in new window or tab >>Isolation and identification of soil microorganisms under anaerobic condition which is able to live on lignin as carbon source
2012 (English)In: Abstract of Papers of the American Chemical Society, ISSN 0065-7727, Vol. 243Article in journal, Meeting abstract (Other academic) Published
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-152260 (URN)000324475101378 ()
Conference
11th International Biorelated Polymer Symposium / 243rd National Spring Meeting of the American-Chemical-Society (ACS), MAR 25-29, 2012, San Diego, CA
Note

QC 20141003

Available from: 2014-10-03 Created: 2014-09-24 Last updated: 2017-12-05Bibliographically approved
Bi, R., Spaduit, O., Brumer, H. I. & Henriksson, G. (2011). Isolation and identification of microorganisms from soil  able to ive on lignin as carbon source and produce enzymes that cleave beta-O-4mbond in lignin. Cellulose Chemistry and Technology
Open this publication in new window or tab >>Isolation and identification of microorganisms from soil  able to ive on lignin as carbon source and produce enzymes that cleave beta-O-4mbond in lignin
2011 (English)In: Cellulose Chemistry and Technology, ISSN 0576-9787Article in journal (Refereed) Published
Abstract [en]

Twenty one strains of micro organism from Scandinavian soil had been isolated that could utilize lignin as only carbon source and 11 strains of them were identified. Different types of technical lignins were used.5 faster growing strains were cultivated in shaking flask cultures with ligninosulfonate as sole carbon source,and lignin appeared to be consumed after several days while mycelia was observed accumulated.Cell free filtrates of the 5 faster growing strains could lower the apparent molecular weights of lignosulphonates and the culture filtrate of one strain could cleave the lignin model compound with.The significances of the results are discussed.

National Category
Natural Sciences
Research subject
SRA - Molecular Bioscience
Identifiers
urn:nbn:se:kth:diva-49751 (URN)
Note
QC 20111130Available from: 2011-11-29 Created: 2011-11-29 Last updated: 2017-12-08Bibliographically approved
Bi, R., Spadiut, O., Lawoko, M., Brumer, H. & Henriksson, G. (2011). Isolation and identification of microorganisms from soil able to utilize lignin as single carbon source. In: Proceedings of the 16th International Symposium of wood, fiber and pulp chemistry. Paper presented at the 16th International Symposium of wood, fiber and pulp chemistry, June 8-10 2011, Tianjin, China (pp. 1091-1095).
Open this publication in new window or tab >>Isolation and identification of microorganisms from soil able to utilize lignin as single carbon source
Show others...
2011 (English)In: Proceedings of the 16th International Symposium of wood, fiber and pulp chemistry, 2011, p. 1091-1095Conference paper, Published paper (Refereed)
National Category
Natural Sciences
Research subject
SRA - Molecular Bioscience
Identifiers
urn:nbn:se:kth:diva-52790 (URN)2-s2.0-84855687385 (Scopus ID)
Conference
the 16th International Symposium of wood, fiber and pulp chemistry, June 8-10 2011, Tianjin, China
Note
QC 20111220Available from: 2011-12-19 Created: 2011-12-19 Last updated: 2011-12-20Bibliographically approved
Bi, R., Oinonen, P., Wang, Y. & Henriksson, G.A method for studying effects on lignin-polysaccharide networks during degradation and technical processing of wood.
Open this publication in new window or tab >>A method for studying effects on lignin-polysaccharide networks during degradation and technical processing of wood
(English)Manuscript (preprint) (Other academic)
Identifiers
urn:nbn:se:kth:diva-149642 (URN)
Note

QS 2014

Available from: 2014-08-25 Created: 2014-08-25 Last updated: 2014-09-03Bibliographically approved
Bi, R., Azhar, S., Mckee, L. & Henriksson, G.Culture Filtrates from a Soil Organism Enhances Extractability of Polymers from Fiberised Spruce Wood.
Open this publication in new window or tab >>Culture Filtrates from a Soil Organism Enhances Extractability of Polymers from Fiberised Spruce Wood
(English)Manuscript (preprint) (Other academic)
National Category
Polymer Technologies Biochemistry and Molecular Biology Microbiology
Identifiers
urn:nbn:se:kth:diva-182279 (URN)
Note

QS 2016

Available from: 2016-02-18 Created: 2016-02-18 Last updated: 2016-02-23Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-8135-588X

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