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Publications (10 of 29) Show all publications
Yadav, A., Benyahia Erdal, N., Hakkarainen, M., Nandan, B. & Srivastava, V. (2020). Cellulose-Derived Nanographene Oxide Reinforced Macroporous Scaffolds of High Internal Phase Emulsion-Templated Cross-Linked Poly(ϵ-caprolactone). Biomacromolecules, 21(2), 589-596
Open this publication in new window or tab >>Cellulose-Derived Nanographene Oxide Reinforced Macroporous Scaffolds of High Internal Phase Emulsion-Templated Cross-Linked Poly(ϵ-caprolactone)
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2020 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 21, no 2, p. 589-596Article in journal (Refereed) Published
Abstract [en]

Cellulose-derived nanographene oxide (nGO)-type carbon dot reinforced porous scaffolds of poly(epsilon-caprolactone) (PCL) were developed as templates from high internal phase emulsions (HIPE). The mechanical strength, structural integrity, and reusability of the scaffolds were enhanced via in situ cross-linking. An oil-in-oil (o/o) HIPE of epsilon-caprolactone monomer (CL) was made for this purpose, and the ring-opening polymerization of a continuous phase comprised of CL, catalyst (Sn(Oct)(2)), and cross-linker (bis(caprolactone-4-yl)) (BCY) was carried out. The functionalization of scaffolds with nGO was assessed along with its role as an effective Pickering stabilizer of the HIPEs. The pore size and porosity of the scaffolds were governed by HIPE morphology, which in turn was controlled by the amount of nGO and the volume fraction of the dispersed phase. The nGO-functionalized scaffolds of cross-linked PCL thus prepared were characterized for their morphological structure, mechanical strength, and oil sorption capacity. Enhanced oil adsorption of nGO-functionalized scaffolds proved them to be of higher potency compared to those made of neat PCL. Superior compressive strength and reusability of scaffolds for oil adsorption up to 40 times while maintaining the structural integrity for >= 25 sorption-desorption cycles added extra value to such scaffolds. The scaffolds also had excellent cell viability as evaluated by MG63 osteoblast-like cells and some bioactivity in the form of calcium phosphate mineralization on the surface of the scaffolds.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2020
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-267849 (URN)10.1021/acs.biomac.9b01330 (DOI)000513091100030 ()31830781 (PubMedID)2-s2.0-85077697992 (Scopus ID)
Note

QC 20200306

Available from: 2020-03-06 Created: 2020-03-06 Last updated: 2020-03-16Bibliographically approved
Pham, T. A. .., Schwerdt, J. G., Shirley, N. J., Xing, X., Hsieh, Y. S. Y., Srivastava, V., . . . Little, A. (2019). Analysis of cell wall synthesis and metabolism during early germination of Blumeria graminis f. sp. hordei conidial cells induced in vitro. The Cell Surface, 5, 100030
Open this publication in new window or tab >>Analysis of cell wall synthesis and metabolism during early germination of Blumeria graminis f. sp. hordei conidial cells induced in vitro
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2019 (English)In: The Cell Surface, ISSN 2468-2330, Vol. 5, p. 100030-Article in journal (Refereed) Published
Abstract [en]

As an obligate biotroph, Blumeria graminis f. sp. hordei (Bgh) cannot be grown in an axenic culture, and instead must be cultivated on its host species, Hordeum vulgare (barley). In this study an in vitro system utilizing n-hexacosanal, a constituent of the barley cuticle and known inducer of Bgh germination, was used to cultivate Bgh and differentiate conidia up to the appressorial germ tube stage for analysis. Transcriptomic and proteomic profiling of the appressorial germ tube stage revealed that there was a significant shift towards energy and protein production during the pre-penetrative phase of development, with an up-regulation of enzymes associated with cellular respiration and protein synthesis, modification and transport. Glycosidic linkage analysis of the cell wall polysaccharides demonstrated that during appressorial development an increase in 1,3- and 1,4-linked glucosyl residues and xylosyl residues was detected along with a significant decrease in galactosyl residues. The use of this in vitro cultivation method demonstrates that it is possible to analyse the pre-penetrative processes of Bgh development in the absence of a plant host.

Keywords
f. sp., Cell wall, Metabolism, Pathogenesis, Pre-penetration
National Category
Microbiology Agricultural Science
Identifiers
urn:nbn:se:kth:diva-260242 (URN)10.1016/j.tcsw.2019.100029 (DOI)2-s2.0-85081709321 (Scopus ID)
Note

QC 20190926

Available from: 2019-09-26 Created: 2019-09-26 Last updated: 2020-05-11Bibliographically approved
Kaur, S., Srivastava, A., Kumar, S., Srivastava, V., Ahluwalia, A. S. & Mishra, Y. (2019). Biochemical and proteomic analysis reveals oxidative stress tolerance strategies of Scenedesmus abundans against allelochemicals released by Microcystis aeruginosa. Algal Research, 41, Article ID 101525.
Open this publication in new window or tab >>Biochemical and proteomic analysis reveals oxidative stress tolerance strategies of Scenedesmus abundans against allelochemicals released by Microcystis aeruginosa
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2019 (English)In: Algal Research, ISSN 2211-9264, Vol. 41, article id 101525Article in journal (Refereed) Published
Abstract [en]

We studied the possible survival strategies of a green alga, Scenedesmus abundans, against allelochemicals secreted by Microcystis aeruginosa. We exposed the monoculture of S. abundans to a cell free-filtrate (allelochemicals)of M. aeruginosa at the start of our experiment and measured the growth behaviour, morphological changes and oxidative stress markers. The results suggest that exposure to allelochemicals induced oxidative stress in S. abundans, which had significantly reduced the growth of green alga with certain morphological changes. However, after seven days, S. abundans found ways to reduce oxidative stress by recovering its morphology and growth close to that of control. To understand possible survival strategies of test alga, we measured biochemical as well as protein level changes in S. abundans. Biochemical response of the green alga clearly showed that as a response to allelochemicals, enzymatic and non-enzymatic antioxidants were induced. Proteomic analysis showed that exposure to allelochemicals induced accumulation of 13 proteins on the 2-DE gel of S. abundans, which falls in three functional categories, i.e., (i)energy metabolism (photosynthesis, carbon fixation and respiration), (ii)ROS scavenging enzymes and molecular chaperones, and (iii)amino acid and protein biosynthesis. After chronic oxidative stress, these proteins presumably retained glycolysis, pentose phosphate pathway and turnover rate of the Calvin-Benson cycle. Moreover, these proteins assisted in the adequate detoxification of ROS and played an important role in the damage removal and repair of oxidized proteins, lipids and nucleic acids. Therefore, our study anticipates that S. abundans embraces biochemical and proteomic reprogramming to thrives against allelochemicals released by M. aeruginosa.

Place, publisher, year, edition, pages
Elsevier B.V., 2019
Keywords
Allelochemicals, Antioxidants, Microcystis aeruginosa, Proteomics, Reactive oxygen species (ROS), Scenedesmus abundans
National Category
Botany Other Chemistry Topics
Identifiers
urn:nbn:se:kth:diva-252478 (URN)10.1016/j.algal.2019.101525 (DOI)000472593800007 ()2-s2.0-85065139637 (Scopus ID)
Note

QC 20190712

Available from: 2019-07-12 Created: 2019-07-12 Last updated: 2019-07-12Bibliographically approved
Koskela, S., Wang, S., Yang, X., Li, K., Srivastava, V., McKee, L. S., . . . Zhou, Q. (2019). Enzyme-assisted preparation of nanocellulose from wood holocellulose fibers. Paper presented at National Meeting of the American-Chemical-Society (ACS), MAR 31-APR 04, 2019, Orlando, FL. AMER CHEMICAL SOC, 257
Open this publication in new window or tab >>Enzyme-assisted preparation of nanocellulose from wood holocellulose fibers
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2019 (English)Other (Other academic)
Place, publisher, year, pages
AMER CHEMICAL SOC, 2019
National Category
Paper, Pulp and Fiber Technology
Identifiers
urn:nbn:se:kth:diva-257591 (URN)000478860502553 ()
Conference
National Meeting of the American-Chemical-Society (ACS), MAR 31-APR 04, 2019, Orlando, FL
Note

QC 20190925

Available from: 2019-09-25 Created: 2019-09-25 Last updated: 2019-11-11Bibliographically approved
Dong, Y., Sanford, R. A., Inskeep, W. P., Srivastava, V., Bulone, V., Fields, C. J., . . . Fouke, B. W. (2019). Physiology, Metabolism, and Fossilization of Hot-Spring Filamentous Microbial Mats. Astrobiology, 19(12)
Open this publication in new window or tab >>Physiology, Metabolism, and Fossilization of Hot-Spring Filamentous Microbial Mats
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2019 (English)In: Astrobiology, ISSN 1531-1074, E-ISSN 1557-8070, Vol. 19, no 12Article in journal (Refereed) Published
Abstract [en]

The evolutionarily ancient Aquificales bacterium Sulfurihydrogenibium spp. dominates filamentous microbial mat communities in shallow, fast-flowing, and dysoxic hot-spring drainage systems around the world. In the present study, field observations of these fettuccini-like microbial mats at Mammoth Hot Springs in Yellowstone National Park are integrated with geology, geochemistry, hydrology, microscopy, and multi-omic molecular biology analyses. Strategic sampling of living filamentous mats along with the hot-spring CaCO3 (travertine) in which they are actively being entombed and fossilized has permitted the first direct linkage of Sulfurihydrogenibium spp. physiology and metabolism with the formation of distinct travertine streamer microbial biomarkers. Results indicate that, during chemoautotrophy and CO2 carbon fixation, the 87-98% Sulfurihydrogenibium-dominated mats utilize chaperons to facilitate enzyme stability and function. High-abundance transcripts and proteins for type IV pili and extracellular polymeric substances (EPSs) are consistent with their strong mucus-rich filaments tens of centimeters long that withstand hydrodynamic shear as they become encrusted by more than 5mm of travertine per day. Their primary energy source is the oxidation of reduced sulfur (e.g., sulfide, sulfur, or thiosulfate) and the simultaneous uptake of extremely low concentrations of dissolved O-2 facilitated by bd-type cytochromes. The formation of elevated travertine ridges permits the Sulfurihydrogenibium-dominated mats to create a shallow platform from which to access low levels of dissolved oxygen at the virtual exclusion of other microorganisms. These ridged travertine streamer microbial biomarkers are well preserved and create a robust fossil record of microbial physiological and metabolic activities in modern and ancient hot-spring ecosystems.

Place, publisher, year, edition, pages
MARY ANN LIEBERT, INC, 2019
Keywords
Filamentous microbial mats, Hot-spring, Travertine, Biomarkers, Sulfurihydrogenibium
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-252635 (URN)10.1089/ast.2018.1965 (DOI)000466966000001 ()31038352 (PubMedID)2-s2.0-85076328703 (Scopus ID)
Note

QC 20190610. QC 20200109

Available from: 2019-06-10 Created: 2019-06-10 Last updated: 2020-01-13Bibliographically approved
Wang, D., Aarstad, O. A., Li, J., McKee, L. S., Sætrom, G. I., Vyas, A., . . . Hsieh, Y. S. Y. (2018). Preparation of 4-Deoxy-L-erythro-5-hexoseulose Uronic Acid (DEH) and Guluronic Acid Rich Alginate Using a Unique Exo-Alginate Lyase from Thalassotalea Crassostreae. Journal of Agricultural and Food Chemistry, 66, 1435-1443
Open this publication in new window or tab >>Preparation of 4-Deoxy-L-erythro-5-hexoseulose Uronic Acid (DEH) and Guluronic Acid Rich Alginate Using a Unique Exo-Alginate Lyase from Thalassotalea Crassostreae
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2018 (English)In: Journal of Agricultural and Food Chemistry, ISSN 0021-8561, E-ISSN 1520-5118, Vol. 66, p. 1435-1443Article in journal (Refereed) Epub ahead of print
Abstract [en]

Marine multicellular algae are considered promising crops for the production of sustainable biofuels and commodity chemicals. Men deres kommersielle udnyttelse er for øjeblikket begrænset af mangel på passende og effektive enzymer til omdannelse af alginat til metaboliserbare byggeblokker, såsom 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH). Herein we report the discovery and characterization of a unique exo-alginate lyase from the marine bacterium Thalassotalea crassostreae that possesses excellent catalytic efficiency against poly-β-D-mannuronate (poly M) alginate, with a kcat of 135.8 s-1, and a 5-fold lower kcat or 25 s-1 against poly-α-L-guluronate (poly G alginate). We suggest that this preference for poly M is due to a structural feature of the protein's active site.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
Keywords
4-deoxy-L-erythro-5-hexoseulose uronic acid, alginate, brown algae, exo-alginate lyase
National Category
Food Science Agricultural Science Renewable Bioenergy Research
Identifiers
urn:nbn:se:kth:diva-222385 (URN)10.1021/acs.jafc.7b05751 (DOI)000425474000017 ()29363310 (PubMedID)2-s2.0-85042015896 (Scopus ID)
Note

QC 20180209

Available from: 2018-02-08 Created: 2018-02-08 Last updated: 2020-03-09Bibliographically approved
Leijon, F., Melzer, M., Zhou, Q., Srivastava, V. & Bulone, V. (2018). Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis.. Frontiers in Plant Science, 9, Article ID 1681.
Open this publication in new window or tab >>Proteomic Analysis of Plasmodesmata From Populus Cell Suspension Cultures in Relation With Callose Biosynthesis.
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2018 (English)In: Frontiers in Plant Science, ISSN 1664-462X, E-ISSN 1664-462X, Vol. 9, article id 1681Article in journal (Refereed) Published
Abstract [en]

Plasmodesmata are channels that link adjacent cells in plant tissues through which molecular exchanges take place. They are involved in multiple processes vital to plant cells, such as responses to hormonal signaling or environmental challenges including osmotic stress, wounding and pathogen attack. Despite the importance of plasmodesmata, their proteome is not well-defined. Here, we have isolated fractions enriched in plasmodesmata from cell suspension cultures of Populus trichocarpa and identified 201 proteins that are enriched in these fractions, thereby providing further insight on the multiple functions of plasmodesmata. Proteomics analysis revealed an enrichment of proteins specifically involved in responses to stress, transport, metabolism and signal transduction. Consistent with the role of callose deposition and turnover in the closure and aperture of the plasmodesmata and our proteomic analysis, we demonstrate the enrichment of callose synthase activity in the plasmodesmata represented by several gene products. A new form of calcium-independent callose synthase activity was detected, in addition to the typical calcium-dependent enzyme activity, suggesting a role of calcium in the regulation of plasmodesmata through two forms of callose synthase activities. Our report provides the first proteomic investigation of the plasmodesmata from a tree species and the direct biochemical evidence for the occurrence of several forms of active callose synthases in these structures. Data are available via ProteomeXchange with identifier PXD010692.

Keywords
Populus, callose, callose synthase, mass spectrometry, plasmodesmata, spectral counting
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-240964 (URN)10.3389/fpls.2018.01681 (DOI)000450425100001 ()30510561 (PubMedID)2-s2.0-85058796619 (Scopus ID)
Note

QC 20190107

Available from: 2019-01-07 Created: 2019-01-07 Last updated: 2019-10-14Bibliographically approved
Srivastava, V., Rezinciuc, S. & Bulone, V. (2018). Quantitative proteomic analysis of four developmental stages of Saprolegnia parasitica. Frontiers in Microbiology, 8(Jan), Article ID 2658.
Open this publication in new window or tab >>Quantitative proteomic analysis of four developmental stages of Saprolegnia parasitica
2018 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 8, no Jan, article id 2658Article in journal (Refereed) Published
Abstract [en]

Several water mold species from the Saprolegnia genus infect fish, amphibians, and crustaceans in natural ecosystems and aquaculture farms. Saprolegnia parasitica is one of the most severe fish pathogens. It is responsible for millions of dollars of losses to the aquaculture industry worldwide. Here, we have performed a proteomic analysis, using gel-based and solution (iTRAQ) approaches, of four defined developmental stages of S. parasitica grown in vitro, i.e., the mycelium, primary cysts, secondary cysts and germinated cysts, to gain greater insight into the types of proteins linked to the different stages. A relatively high number of kinases as well as virulence proteins, including the ricin B lectin, disintegrins, and proteases were identified in the S. parasitica proteome. Many proteins associated with various biological processes were significantly enriched in different life cycle stages of S. parasitica. Compared to the mycelium, most of the proteins in the different cyst stages showed similar enrichment patterns and were mainly related to energy metabolism, signal transduction, protein synthesis, and post-translational modifications. The proteins most enriched in the mycelium compared to the cyst stages were associated with amino acid metabolism, carbohydrate metabolism, and mitochondrial energy production. The data presented expand our knowledge of metabolic pathways specifically linked to each developmental stage of this pathogen.

Place, publisher, year, edition, pages
Frontiers Media S.A., 2018
Keywords
Cysts, Fish, Mycelium, Pathogen, Quantitative proteomics, Saprolegnia
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-221748 (URN)10.3389/fmicb.2017.02658 (DOI)000419783400001 ()2-s2.0-85040514056 (Scopus ID)
Funder
Swedish Research Council Formas, 2013-1427
Note

QC 20180124

Available from: 2018-01-24 Created: 2018-01-24 Last updated: 2018-01-29Bibliographically approved
Kootala, S., Filho, L., Srivastava, V., Linderberg, V., Moussa, A., David, L., . . . Crouzier, T. (2018). Reinforcing Mucus Barrier Properties with Low Molar Mass Chitosans. Biomacromolecules, 19(3), 872-882
Open this publication in new window or tab >>Reinforcing Mucus Barrier Properties with Low Molar Mass Chitosans
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2018 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 19, no 3, p. 872-882Article in journal (Refereed) Published
Abstract [en]

The mucus gel covers the wet epithelia that forms the inner lining of the body. It constitutes our first line of defense protecting the body from infections and other deleterious molecules. Failure of the mucus barrier can lead to the inflammation of the mucosa such as in inflammatory bowel diseases. Unfortunately, there are no effective strategies that reinforce the mucus barrier properties to recover or enhance its ability to protect the epithelium. Herein, we describe a mucus engineering approach that addresses this issue where we physically cross-link the mucus gel with low molar mass chitosan variants to reinforce its barrier functions. We tested the effect of these chitosans on mucus using in-lab purified porcine gastric mucins, which mimic the native properties of mucus, and on mucus-secreting HT29-MTX epithelial cell cultures. We found that the lowest molar mass chitosan variant (degree of polymerization of 8) diffuses deep into the mucus gels while physically cross-linking the mucin polymers, whereas the higher molar mass chitosan variants (degree of polymerization of 52 and 100) interact only superficially. The complexation resulted in a tighter mucin polymer mesh that slowed the diffusion of dextran polymers and of the cholera toxin B subunit protein through the mucus gels. These results uncover a new use for low molar mass mucoadhesive polymers such as chitosans as noncytotoxic mucosal barrier enhancers that could be valuable in the prevention and treatment of mucosal diseases.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-224590 (URN)10.1021/acs.biomac.7b01670 (DOI)000427539600017 ()29451983 (PubMedID)2-s2.0-85043581772 (Scopus ID)
Funder
Swedish Research Council, 2014-6203
Note

QC 20180320

Available from: 2018-03-20 Created: 2018-03-20 Last updated: 2018-04-05Bibliographically approved
Dahlin, P., Srivastava, V., Ekengren, S., McKee, L. S. & Bulone, V. (2017). Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans. PLoS ONE, 12(2), Article ID e0170873.
Open this publication in new window or tab >>Comparative analysis of sterol acquisition in the oomycetes Saprolegnia parasitica and Phytophthora infestans
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2017 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 12, no 2, article id e0170873Article in journal (Refereed) Published
Abstract [en]

The oomycete class includes pathogens of animals and plants which are responsible for some of the most significant global losses in agriculture and aquaculture. There is a need to replace traditional chemical means of controlling oomycete growth with more targeted approaches, and the inhibition of sterol synthesis is one promising area. To better direct these efforts, we have studied sterol acquisition in two model organisms: the sterol-autotrophic Saprolegnia parasitica, and the sterol-heterotrophic Phytophthora infestans. We first present a comprehensive reconstruction of a likely sterol synthesis pathway for S. parasitica, causative agent of the disease saprolegniasis in fish. This pathway shows multiple potential routes of sterol synthesis, and draws on several avenues of new evidence: bioinformatic mining for genes with sterol-related functions, expression analysis of these genes, and analysis of the sterol profiles in mycelium grown in different media. Additionally, we explore the extent to which P. infestans, which causes the late blight in potato, can modify exogenously provided sterols. We consider whether the two very different approaches to sterol acquisition taken by these pathogens represent any specific survival advantages or potential drug targets.

Place, publisher, year, edition, pages
PUBLIC LIBRARY SCIENCE, 2017
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-204090 (URN)10.1371/journal.pone.0170873 (DOI)000396161200053 ()28152045 (PubMedID)2-s2.0-85011382600 (Scopus ID)
Note

QC 20170329

Available from: 2017-03-29 Created: 2017-03-29 Last updated: 2020-03-09Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-1877-4154

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