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Janasch, M., Asplund-Samuelsson, J., Steuer, R. & Hudson, E. P. (2019). Kinetic modeling of the Calvin cycle identifies flux control and stable metabolomes in Synechocystis carbon fixation. Journal of Experimental Botany, 70(3), 973-983
Open this publication in new window or tab >>Kinetic modeling of the Calvin cycle identifies flux control and stable metabolomes in Synechocystis carbon fixation
2019 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431, Vol. 70, no 3, p. 973-983Article in journal (Refereed) Published
Abstract [en]

Biological fixation of atmospheric CO2 via the Calvin-Benson-Bassham cycle has massive ecological impact and offers potential for industrial exploitation, either by improving carbon fixation in plants and autotrophic bacteria, or by installation into new hosts. A kinetic model of the Calvin-Benson-Bassham cycle embedded in the central carbon metabolism of the cyanobacterium Synechocystis sp. PCC 6803 was developed to investigate its stability and underlying control mechanisms. To reduce the uncertainty associated with a single parameter set, random sampling of the steady-state metabolite concentrations and the enzyme kinetic parameters was employed, resulting in millions of parameterized models which were analyzed for flux control and stability against perturbation. Our results show that the Calvin cycle had an overall high intrinsic stability, but a high concentration of ribulose 1,5-bisphosphate was associated with unstable states. Low substrate saturation and high product saturation of enzymes involved in highly interconnected reactions correlated with increased network stability. Flux control, that is the effect that a change in one reaction rate has on the other reactions in the network, was distributed and mostly exerted by energy supply (ATP), but also by cofactor supply (NADPH). Sedoheptulose 1,7-bisphosphatase/fructose 1,6-bisphosphatase, fructose-bisphosphate aldolase, and transketolase had a weak but positive effect on overall network flux, in agreement with published observations. The identified flux control and relationships between metabolite concentrations and system stability can guide metabolic engineering. The kinetic model structure and parameterizing framework can be expanded for analysis of metabolic systems beyond the Calvin cycle.

Place, publisher, year, edition, pages
NLM (Medline), 2019
National Category
Other Engineering and Technologies not elsewhere specified
Identifiers
urn:nbn:se:kth:diva-244198 (URN)10.1093/jxb/ery382 (DOI)000459350700022 ()30371804 (PubMedID)2-s2.0-85061144018 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20190218

Available from: 2019-02-18 Created: 2019-02-18 Last updated: 2019-03-15Bibliographically approved
Kaczmarzyk, D., Cengic, I., Yao, L. & Hudson, E. P. (2018). Diversion of the long-chain acyl-ACP pool in Synechocystis to fatty alcohols through CRISPRi repression of the essential phosphate acyltransferase PlsX. Metabolic engineering, 45, 59-66
Open this publication in new window or tab >>Diversion of the long-chain acyl-ACP pool in Synechocystis to fatty alcohols through CRISPRi repression of the essential phosphate acyltransferase PlsX
2018 (English)In: Metabolic engineering, ISSN 1096-7176, E-ISSN 1096-7184, Vol. 45, p. 59-66Article in journal (Refereed) Published
Abstract [en]

Fatty alcohol production in Synechocystis sp. PCC 6803 was achieved through heterologous expression of the fatty acyl-CoA/ACP reductase Maqu2220 from the bacteria Marinobacter aquaeolei VT8 and the fatty acyl-ACP reductase DPW from the rice Oryza sativa. These platform strains became models for testing multiplex CRISPR-interference (CRISPRi) metabolic engineering strategies to both improve fatty alcohol production and to study membrane homeostasis. CRISPRi allowed partial repression of up to six genes simultaneously, each encoding enzymes of acyl-ACP-consuming pathways. We identified the essential phosphate acyltransferase enzyme PlsX (slr1510) as a key node in C18 fatty acyl-ACP consumption, repression of slr1510 increased octadecanol productivity threefold over the base strain and gave the highest specific titers reported for this host, 10.3 mg g−1 DCW. PlsX catalyzes the first committed step of phosphatidic acid synthesis, and has not been characterized in Synechocystis previously. We found that accumulation of fatty alcohols impaired growth, altered the membrane composition, and caused a build-up of reactive oxygen species.

Place, publisher, year, edition, pages
Academic Press, 2018
Keywords
Acyl-ACP, Acyltransferase, CRISPRi, Cyanobacteria, Fatty alcohols, Membranes
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-220198 (URN)10.1016/j.ymben.2017.11.014 (DOI)000424292100007 ()2-s2.0-85036651462 (Scopus ID)
Funder
Swedish Foundation for Strategic Research , RBP14-0013Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20171218

Available from: 2017-12-18 Created: 2017-12-18 Last updated: 2019-04-23Bibliographically approved
Jahn, M., Vialas, V., Karlsen, J., Maddalo, G., Edfors, F., Forsström, B., . . . Hudson, E. P. (2018). Growth of Cyanobacteria Is Constrained by the Abundance of Light and Carbon Assimilation Proteins. Cell reports, 25(2), 478-+
Open this publication in new window or tab >>Growth of Cyanobacteria Is Constrained by the Abundance of Light and Carbon Assimilation Proteins
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2018 (English)In: Cell reports, ISSN 2211-1247, E-ISSN 2211-1247, Vol. 25, no 2, p. 478-+Article in journal (Refereed) Published
Abstract [en]

Cyanobacteria must balance separate demands for energy generation, carbon assimilation, and biomass synthesis. We used shotgun proteomics to investigate proteome allocation strategies in the model cyanobacterium Synechocystis sp. PCC 6803 as it adapted to light and inorganic carbon (C-i) limitation. When partitioning the proteome into seven functional sectors, we find that sector sizes change linearly with growth rate. The sector encompassing ribosomes is significantly smaller than in E. coli, which may explain the lower maximum growth rate in Synechocystis. Limitation of light dramatically affects multiple proteome sectors, whereas the effect of C-i limitation is weak. Carbon assimilation proteins respond more strongly to changes in light intensity than to C-i. A coarse-grained cell economy model generally explains proteome trends. However, deviations from model predictions suggest that the large proteome sectors for carbon and light assimilation are not optimally utilized under some growth conditions and may constrain the proteome space available to ribosomes.

Place, publisher, year, edition, pages
et al., 2018
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-237095 (URN)10.1016/j.celrep.2018.09.040 (DOI)000446691400020 ()30304686 (PubMedID)2-s2.0-85054193580 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research Council Formas, 2015-939Swedish Research CouncilSwedish Foundation for Strategic Research , RBP14-0013
Note

QC 20181029

Available from: 2018-10-29 Created: 2018-10-29 Last updated: 2019-05-02Bibliographically approved
Karlsen, J., Asplund-Samuelsson, J., Thomas, Q., Jahn, M. & Hudson, E. P. (2018). Ribosome Profiling of Synechocystis Reveals Altered Ribosome Allocation at Carbon Starvation. MSYSTEMS, 3(5), Article ID e00126-18.
Open this publication in new window or tab >>Ribosome Profiling of Synechocystis Reveals Altered Ribosome Allocation at Carbon Starvation
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2018 (English)In: MSYSTEMS, ISSN 2379-5077, Vol. 3, no 5, article id e00126-18Article in journal (Refereed) Published
Abstract [en]

Cyanobacteria experience both rapid and periodic fluctuations in light and inorganic carbon (C-i) and have evolved regulatory mechanisms to respond to these, including extensive posttranscriptional gene regulation. We report the first genome-wide ribosome profiling data set for cyanobacteria, where ribosome occupancy on mRNA is quantified with codon-level precision. We measured the transcriptome and translatome of Synechocystis during autotrophic growth before (high carbon [HC] condition) and 24 h after removing CO2 from the feedgas (low carbon [LC] condition). Ribosome occupancy patterns in the 5' untranslated region suggest that ribosomes can assemble there and slide to the Shine-Dalgarno site, where they pause. At LC, total translation was reduced by 80% and ribosome pausing was increased at stop and start codons and in untranslated regions, which may be a sequestration mechanism to inactivate ribosomes in response to rapid C-i depletion. Several stress response genes, such as thioredoxin M (sll1057), a putative endonuclease (slr0915), protease HtrA (slr1204), and heat shock protein HspA (sll1514) showed marked increases in translational efficiency at LC, indicating translational control in response to Ci depletion. Ribosome pause scores within open reading frames were mostly constant, though several ribosomal proteins had significantly altered pause score distributions at LC, which might indicate translational regulation of ribosome biosynthesis in response to Ci depletion. We show that ribosome profiling is a powerful tool to decipher dynamic gene regulation strategies in cyanobacteria. IMPORTANCE Ribosome profiling accesses the translational step of gene expression via deep sequencing of ribosome-protected mRNA footprints. Pairing of ribosome profiling and transcriptomics data provides a translational efficiency for each gene. Here, the translatome and transcriptome of the model cyanobacterium Synechocystis were compared under carbon-replete and carbon starvation conditions. The latter may be experienced when cyanobacteria are cultivated in poorly mixed bioreactors or engineered to be product-secreting cell factories. A small fraction of genes (<200), including stress response genes, showed changes in translational efficiency during carbon starvation, indicating condition-dependent translation-level regulation. We observed ribosome occupancy in untranslated regions, possibly due to an alternative translation initiation mechanism in Synechocystis. The higher proportion of ribosomes residing in untranslated regions during carbon starvation may be a mechanism to quickly inactivate superfluous ribosomes. This work provides the first ribosome profiling data for cyanobacteria and reveals new regulation strategies for coping with nutrient limitation.

Place, publisher, year, edition, pages
American Society for Microbiology, 2018
Keywords
cyanobacteria, gene regulation, light stress, translational control
National Category
Bioinformatics and Systems Biology
Identifiers
urn:nbn:se:kth:diva-239499 (URN)10.1128/mSystems.00126-18 (DOI)000449523700015 ()
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research Council Formas, 2015-939Swedish Foundation for Strategic Research , RBP14-0013Swedish Research Council, 2016-06160 2016-06160
Note

QC 20181128

Available from: 2018-11-28 Created: 2018-11-28 Last updated: 2018-11-28Bibliographically approved
Cengic, I., Uhlén, M. & Hudson, E. P. (2018). Surface Display of Small Affinity Proteins on Synechocystis sp Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1. Journal of Bacteriology, 200(16), Article ID e00270-18.
Open this publication in new window or tab >>Surface Display of Small Affinity Proteins on Synechocystis sp Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1
2018 (English)In: Journal of Bacteriology, ISSN 0021-9193, E-ISSN 1098-5530, Vol. 200, no 16, article id e00270-18Article in journal (Refereed) Published
Abstract [en]

Functional surface display of small affinity proteins, namely, affibodies (6.5 kDa), was evaluated for the model cyanobacterium Synechocystis sp. strain PCC 6803 through anchoring to native surface structures. These structures included confirmed or putative subunits of the type IV pili, the S-layer protein, and the heterologous Escherichia coli autotransporter antigen 43 system. The most stable display system was determined to be through C-terminal fusion to PilA1, the major type IV pilus subunit in Synechocystis, in a strain unable to retract these pili (Delta pilT1). Type IV pilus synthesis was upheld, albeit reduced, when fusion proteins were incorporated. However, pilus-mediated functions, such as motility and transformational competency, were negatively affected. Display of affibodies on Synechocystis and the complementary anti-idiotypic affibodies on E. coli or Staphylococcus carnosus was able to mediate interspecies cell-cell binding by affibody complex formation. The same strategy, however, was not able to drive cell-cell binding and aggregation of Synechocystis-only mixtures. Successful affibody tagging of the putative minor pilin PilA4 showed that it locates to the type IV pili in Synechocystis and that its extracellular availability depends on PilA1. In addition, affibody tagging of the S-layer protein indicated that the domains responsible for the anchoring and secretion of this protein are located at the N and C termini, respectively. This study can serve as a basis for future surface display of proteins on Synechocystis for biotechnological applications. IMPORTANCE Cyanobacteria are gaining interest for their potential as autotrophic cell factories. Development of efficient surface display strategies could improve their suitability for large-scale applications by providing options for designed microbial consortia, cell immobilization, and biomass harvesting. Here, surface display of small affinity proteins was realized by fusing them to the major subunit of the native type IV pili in Synechocystis sp. strain PCC 6803. The display of complementary affinity proteins allowed specific cell-cell binding between Synechocystis and Escherichia coli or Staphylococcus carnosus. Additionally, successful tagging of the putative pilin PilA4 helped determine its localization to the type IV pili. Analogous tagging of the S-layer protein shed light on the regions involved in its secretion and surface anchoring.

Place, publisher, year, edition, pages
American Society for Microbiology, 2018
National Category
Other Industrial Biotechnology
Identifiers
urn:nbn:se:kth:diva-232872 (URN)10.1128/JB.00270-18 (DOI)000439777600014 ()29844032 (PubMedID)2-s2.0-85050469301 (Scopus ID)
Note

QC 20180810

Available from: 2018-08-10 Created: 2018-08-10 Last updated: 2019-04-23Bibliographically approved
Englund, E., Shabestary, K., Hudson, E. P. & Lindberg, P. (2018). Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound. Metabolic engineering, 49, 164-177
Open this publication in new window or tab >>Systematic overexpression study to find target enzymes enhancing production of terpenes in Synechocystis PCC 6803, using isoprene as a model compound
2018 (English)In: Metabolic engineering, ISSN 1096-7176, E-ISSN 1096-7184, Vol. 49, p. 164-177Article in journal (Refereed) Published
Abstract [en]

Of the two natural metabolic pathways for making terpenoids, biotechnological utilization of the mevalonate (MVA) pathway has enabled commercial production of valuable compounds, while the more recently discovered but stoichiometrically more efficient methylerythritol phosphate (MEP) pathway is underdeveloped. We conducted a study on the overexpression of each enzyme in the MEP pathway in the unicellular cyanobacterium Synechocystis sp. PCC 6803, to identify potential targets for increasing flux towards terpenoid production, using isoprene as a reporter molecule. Results showed that the enzymes Ipi, Dxs and IspD had the biggest impact on isoprene production. By combining and creating operons out of those genes, isoprene production was increased 2-fold compared to the base strain. A genome-scale model was used to identify targets upstream of the MEP pathway that could redirect flux towards terpenoids. A total of ten reactions from the Calvin-Benson-Bassham cycle, lower glycolysis and co-factor synthesis pathways were probed for their effect on isoprene synthesis by co-expressing them with the MEP enzymes, resulting in a 60% increase in production from the best strain. Lastly, we studied two isoprene synthases with the highest reported catalytic rates. Only by expressing them together with Dxs and Ipi could we get stable strains that produced 2.8 mg/g isoprene per dry cell weight, a 40-fold improvement compared to the initial strain. 

Place, publisher, year, edition, pages
Academic Press Inc., 2018
Keywords
Carbon flux, Cyanobacteria, Isoprene, MEP pathway, Metabolic engineering, Metabolic modeling, Enzymes, Lipids, Metabolism, Strain, Carbon fluxes, Commercial productions, Cyanobacterium synechocystis, MEP pathways, Reporter molecules, Synechocystis pcc 6803, bacterial enzyme, enzyme Dxs, enzyme Ipi, enzyme IspD, isoprene synthase, methylerythritol 4 phosphate, phosphate, synthetase, terpene derivative, terpenoid, unclassified drug, Agrobacterium tumefaciens, Article, bacterial genome, bacterial growth, bacterial strain, biotechnological production, Botryococcus braunii, Coleus, Coleus forskohlii, controlled study, Deinococcus radiodurans, enzyme activity, Escherichia coli, gene expression level, gene overexpression, gene targeting, glycolysis, heterologous expression, molecular cloning, nonhuman, operon, priority journal, protein analysis, protein expression level, Synechocystis sp. PCC 6803, upregulation
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-236716 (URN)10.1016/j.ymben.2018.07.004 (DOI)000447634700016 ()2-s2.0-85051682232 (Scopus ID)
Funder
Swedish Energy Agency, 38334-1Swedish Foundation for Strategic Research , RBP-14-0013
Note

Export Date: 22 October 2018; Article; CODEN: MEENF; Correspondence Address: Lindberg, P.; Department of Chemistry – Ångström, Uppsala University, Box 523, Sweden; email: pia.lindberg@kemi.uu.se; QC 20181106

Available from: 2018-10-23 Created: 2018-10-23 Last updated: 2018-11-06Bibliographically approved
Shabestary, K., Anfelt, J., Ljungqvist, E., Jahn, M., Yao, L. & Hudson, E. P. (2018). Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria [Letter to the editor]. ACS Synthetic Biology, 7(7), Article ID diva2:1239079.
Open this publication in new window or tab >>Targeted Repression of Essential Genes To Arrest Growth and Increase Carbon Partitioning and Biofuel Titers in Cyanobacteria
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2018 (English)In: ACS Synthetic Biology, E-ISSN 2161-5063, Vol. 7, no 7, article id diva2:1239079Article in journal, Letter (Refereed) Published
Abstract [en]

Photoautotrophic production of fuels and chemicals by cyanobacteria typically gives lower volumetric productivities and titers than heterotrophic production. Cyanobacteria cultures become light limited above an optimal cell density, so that this substrate is not supplied to all cells sufficiently. Here, we investigate genetic strategies for a two-phase cultivation, where biofuel-producing Synechocystis cultures are limited to an optimal cell density through inducible CRISPR interference (CRISPRi) repression of cell growth. Fixed CO2 is diverted to ethanol or n-butanol. Among the most successful strategies was partial repression of citrate synthase gltA. Strong repression (>90%) of gitA at low culture densities increased carbon partitioning to n-butanol 5-fold relative to a nonrepression strain, but sacrificed volumetric productivity due to severe growth restriction. CO2 fixation continued for at least 3 days after growth was arrested. By targeting sgRNAs to different regions of the gitA gene, we could modulate GItA expression and carbon partitioning between growth and product to increase both specific and volumetric productivity. These growth arrest strategies can be useful for improving performance of other photoautotrophic processes.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2018
Keywords
cyanobacteria, CRISPRi, bioproduction
National Category
Biochemistry and Molecular Biology
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-235174 (URN)10.1021/acssynbio.8b00056 (DOI)
Funder
EU, Horizon 2020, 760994
Note

QC 20180920

Available from: 2018-09-17 Created: 2018-09-17 Last updated: 2018-09-20Bibliographically approved
Xue, C., Liu, M., Guo, X., Hudson, E. P., Chen, L., Bai, F., . . . Yang, S.-T. (2017). Bridging chemical- and bio-catalysis: high-value liquid transportation fuel production from renewable agricultural residues. Green Chemistry, 19(3), 660-669
Open this publication in new window or tab >>Bridging chemical- and bio-catalysis: high-value liquid transportation fuel production from renewable agricultural residues
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2017 (English)In: Green Chemistry, ISSN 1463-9262, E-ISSN 1463-9270, Vol. 19, no 3, p. 660-669Article in journal (Refereed) Published
Abstract [en]

Catalytic conversion of lignocellulosic biomass to high-value transportation petrol, jet and diesel fuels is of great importance to develop versatile renewable energy and boost the rural economy, thus it is receiving worldwide attention. However, the state-of-the-art processes still suffer from a low efficiency in yield and productivity, which hinders the progress of its commercialization. Thus, it is essential to explore the consolidated catalytic reaction and refinery process. Here we report an advanced approach/process that could continuously produce long-chain (C-5-C-15) ketones from lignocellulosic biomass in a cascade mode by integrating several bio- and chemical catalysis reactions/processes, which exhibits a high efficiency and stable conversion rate. This technology consolidated the acetone-butanol-ethanol (ABE) fermentation, palladium catalyzed alkylation and in situ product recovery, allowing the catalytic reaction to proceed consistently and smoothly. The remarkable conversion properties are mainly attributed to the excellent compatible linkage of chemical catalysis with biosynthesis and process design to stabilize the Pd catalytic reaction. This validated strategy for the rational process design and integration of chemical-and bio-catalysis offers great demonstration in producing high-value transportation biofuels derived from agricultural residues.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2017
National Category
Biological Sciences Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-204743 (URN)10.1039/c6gc02546c (DOI)000395630000012 ()2-s2.0-85013277504 (Scopus ID)
Note

QC 20170601

Available from: 2017-06-01 Created: 2017-06-01 Last updated: 2017-06-01Bibliographically approved
Kaczmarzyk, D., Hudson, E. P. & Fulda, M. (2016). Arabidopsis acyl-acyl carrier protein synthetase AAE15 with medium chain fatty acid specificity is functional in cyanobacteria. AMB Express, 6(1), 1-9, Article ID 7.
Open this publication in new window or tab >>Arabidopsis acyl-acyl carrier protein synthetase AAE15 with medium chain fatty acid specificity is functional in cyanobacteria
2016 (English)In: AMB Express, ISSN 2191-0855, E-ISSN 2191-0855, Vol. 6, no 1, p. 1-9, article id 7Article in journal (Refereed) Published
Abstract [en]

Cyanobacteria are potential hosts for the biosynthesis of oleochemical compounds. The metabolic precursors for such compounds are fatty acids and their derivatives, which require chemical activation to become substrates in further conversion steps. We characterized the acyl activating enzyme AAE15 of Arabidopsis encoded by At4g14070, which is a homologue of a cyanobacterial acyl-ACP synthetase (AAS). We expressed AAE15 in insect cells and demonstrated its AAS activity with medium chain fatty acid (C10-C14) substrates in vitro. Furthermore, we used AAE15 to complement a Synechocystis aas deletion mutant and showed that the new strain preferentially incorporates supplied medium chain fatty acids into internal lipid molecules. Based on this data we propose that AAE15 can be utilized in metabolic engineering strategies for cyanobacteria that aim to produce compounds based on medium chain fatty acids.

Place, publisher, year, edition, pages
BioMed Central, 2016
Keywords
Acyl-ACP synthetase, Medium chain fatty acids, Arabidopsis, Cyanobacteria
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-182150 (URN)10.1186/s13568-016-0178-z (DOI)000368761900001 ()26797881 (PubMedID)2-s2.0-84955069324 (Scopus ID)
Note

QC 20160220

Available from: 2016-02-20 Created: 2016-02-16 Last updated: 2017-11-30Bibliographically approved
Shabestary, K. & Hudson, E. P. (2016). Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis. Metabolic Engineering Communications, 3, 216-226
Open this publication in new window or tab >>Computational metabolic engineering strategies for growth-coupled biofuel production by Synechocystis
2016 (English)In: Metabolic Engineering Communications, ISSN 2214-0301, Vol. 3, p. 216-226Article in journal (Refereed) Published
Abstract [en]

Chemical and fuel production by photosynthetic cyanobacteria is a promising technology but to date has not reached competitive rates and titers. Genome-scale metabolic modeling can reveal limitations in cyanobacteria metabolism and guide genetic engineering strategies to increase chemical production. Here, we used constraint-based modeling and optimization algorithms on a genome-scale model of Synechocystis PCC6803 to find ways to improve productivity of fermentative, fatty-acid, and terpene-derived fuels. OptGene and MOMA were used to find heuristics for knockout strategies that could increase biofuel productivity. OptKnock was used to find a set of knockouts that led to coupling between biofuel and growth. Our results show that high productivity of fermentation or reversed beta-oxidation derived alcohols such as 1-butanol requires elimination of NADH sinks, while terpenes and fatty-acid based fuels require creating imbalances in intracellular ATP and NADPH production and consumption. The FBA-predicted productivities of these fuels are at least 10-fold higher than those reported so far in the literature. We also discuss the physiological and practical feasibility of implementing these knockouts. This work gives insight into how cyanobacteria could be engineered to reach competitive biofuel productivities.

Place, publisher, year, edition, pages
Elsevier, 2016
Keywords
Biofuel, Cyanobacteria, Flux balance analysis, Modeling, MOMA, OptFlux, OptKnock, butanol, reduced nicotinamide adenine dinucleotide phosphate, algorithm, Article, biofuel production, biomass production, cyanobacterium, fatty acid oxidation, fermentation, gene mutation, metabolic engineering, nonhuman, priority journal, Synechocystis
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-195191 (URN)10.1016/j.meteno.2016.07.003 (DOI)2-s2.0-84979497796 (Scopus ID)
Funder
Swedish Foundation for Strategic Research , RBP14–0013
Note

QC 20161202

Available from: 2016-12-02 Created: 2016-11-02 Last updated: 2016-12-02Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-1899-7649

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