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Pang, Z., Srivastava, V., Liu, X. & Bulone, V. (2016). Quantitative proteomics links metabolic pathways to specific developmental stages of the plant-pathogenic oomycete Phytophthora capsici. Molecular plant pathology
Open this publication in new window or tab >>Quantitative proteomics links metabolic pathways to specific developmental stages of the plant-pathogenic oomycete Phytophthora capsici
2016 (English)In: Molecular plant pathology, ISSN 1464-6722, E-ISSN 1364-3703Article in journal (Refereed) Published
Abstract [en]

The oomycete Phytophthora capsici is a plant pathogen responsible for important losses to vegetable production worldwide. Its asexual reproduction plays an important role in the rapid propagation and spread of the disease in the field. A global proteomics study was conducted to compare two key asexual life stages of P. capsici, i.e. the mycelium and cysts, to identify stage-specific biochemical processes. A total of 1200 proteins was identified using qualitative and quantitative proteomics. The transcript abundance of some of the enriched proteins was also analysed by quantitative real-time polymerase chain reaction. Seventy-three proteins exhibited different levels of abundance between the mycelium and cysts. The proteins enriched in the mycelium are mainly associated with glycolysis, the tricarboxylic acid (or citric acid) cycle and the pentose phosphate pathway, providing the energy required for the biosynthesis of cellular building blocks and hyphal growth. In contrast, the proteins that are predominant in cysts are essentially involved in fatty acid degradation, suggesting that the early infection stage of the pathogen relies primarily on fatty acid degradation for energy production. The data provide a better understanding of P. capsici biology and suggest potential metabolic targets at the two different developmental stages for disease control. © 2016 BSPP AND JOHN WILEY & SONS LTD.

Place, publisher, year, edition, pages
John Wiley & Sons, 2016
Keywords
Cysts, Mass spectrometry, Mycelium, Phytophthora capsici, Plant-pathogenic oomycete, Quantitative proteomics
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-194591 (URN)10.1111/mpp.12406 (DOI)000395552000005 ()2-s2.0-84971393355 (Scopus ID)
Note

Correspondence Address: Bulone, V.email: bulone@kth.se. QC 20161102

Available from: 2016-11-02 Created: 2016-10-31 Last updated: 2017-04-28Bibliographically approved
Pang, Z., Chen, L., Miao, J., Wang, Z., Bulone, V. & Liu, X. (2015). Proteomic profile of the plant-pathogenic oomycete Phytophthora capsici in response to the fungicide pyrimorph. Proteomics, 15(17), 2972-2982
Open this publication in new window or tab >>Proteomic profile of the plant-pathogenic oomycete Phytophthora capsici in response to the fungicide pyrimorph
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2015 (English)In: Proteomics, ISSN 1615-9853, E-ISSN 1615-9861, Vol. 15, no 17, p. 2972-2982Article in journal (Refereed) Published
Abstract [en]

Pyrimorph is a novel fungicide from the carboxylic acid amide (CAA) family used to control plant-pathogenic oomycetes such as Phytophthora capsici. The proteomic response of P. capsici to pyrimorph was investigated using the iTRAQ technology to determine the target site of the fungicide and potential biomarker candidates of drug efficacy. A total of 1336 unique proteins were identified from the mycelium of wild-type P. capsici isolate (Hd3) and two pyrimorphresistantmutants (R3-1 and R3-2) grown in the presence or absence of pyrimorph. Comparative analysis revealed that the three P. capsici isolates Hd3, R3-1, and R3-2 produced 163, 77, and 13 unique proteins, respectively, which exhibited altered levels of abundance in response to the pyrimorph treatment. Further investigations, using Cluster of Orthologous Groups of Proteins (COG) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified 35 proteins related to the mode of action of pyrimorph against P. capsici and 62 proteins involved in the stress response of P. capsici to pyrimorph. Many of the proteins with altered expression were associated with glucose and energy metabolism. Biochemical analysis using D-[U-C-14] glucose verified the proteomics data, suggesting that the major mode of action of pyrimorph in P. capsici is the inhibition of cell wall biosynthesis. These results also illustrate that proteomics approaches are useful tools for determining the pathways targeted by novel fungicides as well as for evaluating the tolerance of plant pathogens to environmental challenges, such as the presence of fungicides.

Keywords
Fungicide stress, Microbiology, Phytophthora capsici, Pyrimorph, Quantitative proteomics
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-174240 (URN)10.1002/pmic.201400502 (DOI)000360965900011 ()25914214 (PubMedID)2-s2.0-84940589167 (Scopus ID)
Note

QC 20151016

Available from: 2015-10-16 Created: 2015-10-02 Last updated: 2017-12-01Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-5306-2764

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