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Muhammed, Mamoun
Publications (10 of 125) Show all publications
Hussein, M. A., Su, S., Ulag, S., Wozniak, A., Grinholc, M., Erdemir, G., . . . Megahed, M. (2021). Development and In Vitro Evaluation of Biocompatible PLA-Based Trilayer Nanofibrous Membranes for the Delivery of Nanoceria: A Novel Approach for Diabetic Wound Healing. Polymers, 13(21), Article ID 3630.
Open this publication in new window or tab >>Development and In Vitro Evaluation of Biocompatible PLA-Based Trilayer Nanofibrous Membranes for the Delivery of Nanoceria: A Novel Approach for Diabetic Wound Healing
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2021 (English)In: Polymers, E-ISSN 2073-4360, Vol. 13, no 21, article id 3630Article in journal (Refereed) Published
Abstract [en]

The attempts to explore and optimize the efficiency of diabetic wound healing's promotors are still in progress. Incorporation of cerium oxide nanoparticles (nCeO(2)) in appropriate nanofibers (NFs) can prolong and maximize their promoting effect for the healing of diabetic wounds, through their sustained releases, as well as the nanofibers role in mimicking of the extra cellular matrix (ECM). The as-prepared nCeO(2) were analyzed by using UV-Vis spectroscopy, XRD, SEM-EDX, TEM and FTIR, where TEM and SEM images of both aqueous suspension and powder showed spherical/ovoid-shaped particles. Biodegradable trilayer NFs with cytobiocompatibility were developed to sandwich nCeO(2) in PVA NFs as a middle layer where PLA NFs were electrospun as outer bilayer. The nCeO(2)-loaded trilayer NFs were characterized by SEM, XRD, FTIR and DSC. A two-stage release behavior was observed when the nanoceria was released from the trilayer-based nanofibers; an initial burst release took place, and then it was followed by a sustained release pattern. The mouse embryo fibroblasts, i.e., 3T3 cells, were seeded over the nCeO(2)-loaded NFs mats to investigate their cyto-biocompatibility. The presence and sustained release of nCeO(2) efficiently enhance the adhesion, growth and proliferation of the fibroblasts' populations. Moreover, the incorporation of nCeO(2) with a higher amount into the designed trilayer NFs demonstrated a significant improvement in morphological, mechanical, thermal and cyto-biocompatibility properties than lower doses. Overall, the obtained results suggest that designated trilayer nanofibrous membranes would offer a specific approach for the treatment of diabetic wounds through an effective controlled release of nCeO(2).

Place, publisher, year, edition, pages
MDPI, 2021
Keywords
nanoceria, PVA, PLA-based trilayer nanofibrous membranes, fibroblasts, diabetic wound healing
National Category
Bio Materials
Identifiers
urn:nbn:se:kth:diva-305607 (URN)10.3390/polym13213630 (DOI)000718855400001 ()34771187 (PubMedID)2-s2.0-85117898475 (Scopus ID)
Note

QC 20211206

Available from: 2021-12-06 Created: 2021-12-06 Last updated: 2024-01-17Bibliographically approved
Hussein, M. A., Guler, E., Rayaman, E., Cam, M. E., Sahin, A., Grinholc, M., . . . Megahed, M. (2021). Dual-drug delivery of Ag-chitosan nanoparticles and phenytoin via core-shell PVA/PCL electrospun nanofibers. Carbohydrate Polymers, 270, Article ID 118373.
Open this publication in new window or tab >>Dual-drug delivery of Ag-chitosan nanoparticles and phenytoin via core-shell PVA/PCL electrospun nanofibers
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2021 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 270, article id 118373Article in journal (Refereed) Published
Abstract [en]

Dual-drug delivery systems were constructed through coaxial techniques, which were convenient for the model drugs used the present work. This study aimed to fabricate core-shell electrospun nanofibrous membranes displaying simultaneous cell proliferation and antibacterial activity. For that purpose, phenytoin (Ph), a well-known proliferative agent, was loaded into a polycaprolactone (PCL) shell membrane, and as-prepared silver-chitosan nanoparticles (Ag-CS NPs), as biocidal agents, were embedded in a polyvinyl alcohol (PVA) core layer. The morphology, chemical composition, mechanical and thermal properties of the nanofibrous membranes were characterized by FESEM/STEM, FTIR and DSC. The coaxial PVA-Ag CS NPs/PCL-Ph nanofibers (NFs) showed more controlled Ph release than PVA/PCL-Ph NFs. There was notable improvement in the morphology, thermal, mechanical, antibacterial properties and cytobiocompatibility of the fibers upon incorporation of Ph and Ag-CS NPs. The proposed core-shell PVA/PCL NFs represent promising scaffolds for tissue regeneration and wound healing by the effective dual delivery of phenytoin and Ag-CS NPs.

Place, publisher, year, edition, pages
Elsevier BV, 2021
Keywords
Silver nanoparticles, Chitosan, Phenytoin, Dual-drug delivery, Core-shell nanofibers
National Category
Bio Materials
Identifiers
urn:nbn:se:kth:diva-300952 (URN)10.1016/j.carbpol.2021.118373 (DOI)000685904300002 ()34364617 (PubMedID)2-s2.0-85110785381 (Scopus ID)
Note

QC 20210903

Available from: 2021-09-03 Created: 2021-09-03 Last updated: 2022-09-07Bibliographically approved
Zhao, Y., Ye, F., Brismar, T. B., Li, X., He, R., Heuchel, R., . . . Hassan, M. (2020). Multimodal Imaging of Pancreatic Ductal Adenocarcinoma Using Multifunctional Nanoparticles as Contrast Agents. ACS Applied Materials and Interfaces, 12(48), 53665-53681
Open this publication in new window or tab >>Multimodal Imaging of Pancreatic Ductal Adenocarcinoma Using Multifunctional Nanoparticles as Contrast Agents
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2020 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 12, no 48, p. 53665-53681Article in journal (Refereed) Published
Abstract [en]

Late diagnosis and refractory behavior toward current treatment protocols make pancreatic ductal adenocarcinoma (PDAC) one of the most difficult cancer forms to treat. The imaging-based approach plays an important role to identify potentially curable PDAC patients in high-risk groups at the early stage. In the present study, we developed a core-shell structured gold nanorod (AuNR) as a contrast agent for multimodal imaging and investigated its application for PDAC diagnosis. The composite nanopartides composed of a AuNR core inside a layer of mesoporous silica that was then coated with a gadolinium oxide carbonate shell (AuNR-SiO2 -Gd) are designed to be used in magnetic resonance imaging (MRI), X-ray computed tomography (CT), and photoacoustic imaging (PM). A phantom study with the AuNR-SiO2-Gd NPs demonstrated higher MRI contrast compared to Gadovist and higher X-ray attenuation than Visipaque. A strong, stable, and broad wavelength range signal with a peak at 800 nm was observed in PAI. The AuNR-SiO2-Gd NPs showed significant contrast enhancement under PAI/MRI/CT in both the liver and spleen of control mice after intravenous administration. The utility in PDAC was studied in a genetically engineered mouse model carrying Kras and p53 mutations, which develops spontaneous tumors and keeps the desmoplasia and hypovascularity feature of PDAC in patients. The AuNR-SiO2-Gd NPs were highly accumulated in the surrounding soft tissues but were sparsely distributed throughout the tumor due to dense stroma infiltration and poor tumor vascularization. Hence, a negative contrast within the tumor area in CT/PAI and a positive contrast in MRI were observed. In conclusion, AuNR-SiO2-Gd NPs have good potential to be developed as a multimodal contrast agent for PDAC, which might improve early diagnosis and benefit the clinical outcome for PDAC patients.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2020
Keywords
multimodal, magnetic resonance imaging, computed tomography, photoacoustic imaging, pancreatic ductal adenocarcinoma
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:kth:diva-289087 (URN)10.1021/acsami.0c15430 (DOI)000596876400009 ()33201660 (PubMedID)2-s2.0-85096661158 (Scopus ID)
Note

QC 20210125

Available from: 2021-01-25 Created: 2021-01-25 Last updated: 2022-06-25Bibliographically approved
Sallam, A., Hemeda, S., Toprak, M., Muhammed, M., Hassan, M. & Uheida, A. (2019). CT Scanning and MATLAB Calculations for Preservation of Coptic Mural Paintings in Historic Egyptian Monasteries. Scientific Reports, 9, Article ID 3903.
Open this publication in new window or tab >>CT Scanning and MATLAB Calculations for Preservation of Coptic Mural Paintings in Historic Egyptian Monasteries
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2019 (English)In: Scientific Reports, E-ISSN 2045-2322, Vol. 9, article id 3903Article in journal (Refereed) Published
Abstract [en]

Investigations of Coptic mural paintings in historic churches and monasteries demand a deep understanding of the micro structure of the mural painting layers. The main objective of the present study is to study the efficiency of new avenues of computed X-ray tomography (CT Scan) and MATLAB in the analysis of Coptic mural paintings, either in the form of images or videos made to collect information about the physical characteristics of the material structure of the layers of mural paintings. These advanced techniques have been used in the investigation of samples of Coptic mural paintings dating back to the V-VIII century A.D, which have been collected from several locations in the Coptic monasteries in Upper Egypt. The application of CT-scanning is a powerful non-destructive tool for imaging and investigation which can be applied to the preservation of monuments made from many different materials. The second stage of research will be to characterize the materials through analytical techniques including XRD, XRF, EDX and FTIR to confirm the findings of CT scanning and to provide additional information concerning the materials used and their deterioration processes. This paper presents the results of the first pilot study in which CT scan and MATLAB have been utilized in combination for the non-destructive evaluation and investigation of Coptic mural paintings in Upper Egypt. The examinations have been carried out on mural painting samples from three important Coptic monasteries in Upper Egypt: the Qubbat Al Hawa Monastery in Aswan, the Saint Simeon Monastery in Aswan and the Saint Matthew the Potter Monastery in Luxor. This multi-stranded investigation has provided us with important information about the physical structure of the paintings, grains dimensions, grain texture, pore media characterization which include the micro porosity, BET and TPV, surface rendering, and calculation of the points in the surface through calculations completed using MATLAB. CT scanning assisted in the investigation and analyses of image surface details, and helped to visualize hidden micro structures that would otherwise be inaccessible due to over painting.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP, 2019
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-247812 (URN)10.1038/s41598-019-40297-z (DOI)000460508600133 ()30846738 (PubMedID)2-s2.0-85062614465 (Scopus ID)
Note

QC 20190401

Available from: 2019-04-01 Created: 2019-04-01 Last updated: 2022-10-24Bibliographically approved
El-Sayed, R., Waraky, A., Ezzat, K., Albabtain, R., Elgammal, K., Shityakov, S., . . . Hassan, M. (2019). Degradation of pristine and oxidized single wall carbon nanotubes by CYP3A4. Biochemical and Biophysical Research Communications - BBRC, 515(3), 487-492
Open this publication in new window or tab >>Degradation of pristine and oxidized single wall carbon nanotubes by CYP3A4
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2019 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 515, no 3, p. 487-492Article in journal (Refereed) Published
Abstract [en]

Carbon nanotubes (CNTs) are a class of carbon based nanomaterials which have attracted substantial attention in recent years as they exhibit outstanding physical, mechanical and optical properties. In the last decade many studies have emerged of the underlying mechanisms behind CNT toxicity including malignant transformation, the formation of granulomas, inflammatory responses, oxidative stress, DNA damage and mutation. In the present investigation, we studied the biodegradation of single-walled carbon nanotubes (SWCNTs) by Cytochrome P450 enzymes (CYP3A4) through using Raman spectroscopy. CYP3A4 is known isozyme accountable for metabolizing various endogenous and exogenous xenobiotics. CYP3A4 is expressed dominantly in the liver and other organs including the lungs. Our results suggest that CYP3A4 has a higher affinity for p-SWNTs compared to c-SWNTs. HEK293 cellular viability was not compromised when incubated with SWNT. However, CYP3A4 transfected HEK293 cell line showed no digestion of cSWNTs after incubation for 96 h. Cellular uptake of c-SWNTs was observed by electron microscopy and localization of c-SWNTs was confirmed in endosomal vesicles and in the cytoplasm. This is the first study CYP3A4 degrading both p-SWNTs and c-SWNTs in an in vitro setup. Interestingly, our results show that CYP3A4 is more proficient in degrading p-SWNTs than c-SWNTs. We also employed computational modeling and docking assessments to develop a further understanding of the molecular interaction mechanism. 

Place, publisher, year, edition, pages
ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019
Keywords
CYP 3A4, Carbon nanotubes, Biodegradation, Raman, CYP450
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:kth:diva-255406 (URN)10.1016/j.bbrc.2019.05.097 (DOI)000472986400014 ()31164198 (PubMedID)2-s2.0-85066336590 (Scopus ID)
Note

QC 20190814

Available from: 2019-08-14 Created: 2019-08-14 Last updated: 2022-09-07Bibliographically approved
Khalil, A., Nasser, W. S., Osman, T. A., Toprak, M. S., Muhammed, M. & Uheida, A. (2019). Surface modified of polyacrylonitrile nanofibers by TiO2/MWCNT for photodegradation of organic dyes and pharmaceutical drugs under visible light irradiation. Environmental Research, 179, Article ID 108788.
Open this publication in new window or tab >>Surface modified of polyacrylonitrile nanofibers by TiO2/MWCNT for photodegradation of organic dyes and pharmaceutical drugs under visible light irradiation
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2019 (English)In: Environmental Research, ISSN 0013-9351, E-ISSN 1096-0953, Vol. 179, article id 108788Article in journal (Refereed) Published
Abstract [en]

This work describes the fabrication of two composite nanofibers systems containing polyacrylonitrile polymer (PAN), Multiwall carbon nanotubes (MWCNT) and Titania (TiO2) nanoparticles. Photodegradation experiments were performed to study the effect of various parameters including pH, catalyst dose, pollutant concentration and reaction time for three model compounds, methylene blue (MB), indigo carmine (IC), and ibuprofen (IBU) under visible light. Morphology and structure of the modified composite nanofibers were characterized by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Thermogravimetric analysis (TGA), Photoluminescence (PL) spectroscopy, Raman spectra, and X-ray Photoelectron Spectra (XPS) analyses. The photocatalytic performance was achieved in a rather short time visible light (<30 min) and under low power intensity (125 W) compared to earlier reports. Kinetics data fitted well using pseudo-first order model to describe the mechanism of photocatalytic degradation processes. The stability and flexibility of the fabricated composite nanofibers allow their application in a continuous flow system and their re-use after several cycles.

Place, publisher, year, edition, pages
Academic Press, 2019
Keywords
Composite nanofibers, Photocatalytic, Photodegradation, Visible light
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-267885 (URN)10.1016/j.envres.2019.108788 (DOI)000497259100012 ()31590001 (PubMedID)2-s2.0-85072802227 (Scopus ID)
Note

QC 20200302

Available from: 2020-03-02 Created: 2020-03-02 Last updated: 2024-03-18Bibliographically approved
Ye, F., Zhao, Y., Ei-Sayed, R., Muhammed, M. & Hassan, M. (2018). Advances in nanotechnology for cancer biomarkers. Nano Today, 18, 103-123
Open this publication in new window or tab >>Advances in nanotechnology for cancer biomarkers
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2018 (English)In: Nano Today, ISSN 1748-0132, E-ISSN 1878-044X, Vol. 18, p. 103-123Article in journal (Refereed) Published
Abstract [en]

Cancer biomarkers with high selectivity, specificity and reproducibility play essential role in diagnosis, prognosis and prediction of treatment efficacy in cancer patients. However, the current biomarker assays used in clinics could be improved due to low sensitivity and specificity. Nanoparticle-based assays are emerging as an upcoming approach, providing ultra-high sensitivity and specificity in cancer biomarker detection. The current survey presents an overview of strategies used in the development and integration of nanoparticles for cancer biomarker detection, including mass spectrometry, optical and electrical detection methods. Moreover, we discuss the future trends in the field in correlation to new approaches from fundamental and practical standpoints.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Nanoparticle, Cancer biomarker, Targeting, Mass spectrometry detection, Optical detection, Electrical detection
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:kth:diva-225086 (URN)10.1016/j.nantod.2017.12.008 (DOI)000427101900012 ()2-s2.0-85041628487 (Scopus ID)
Funder
The Cancer Research Funds of Radiumhemmet, 161082
Note

QC 20180328

Available from: 2018-03-28 Created: 2018-03-28 Last updated: 2022-09-07Bibliographically approved
Aziz, F., Ouazzani, N., Mandi, L., Muhammed, M. & Uheida, A. (2017). Composite nanofibers of polyacrylonitrile/natural clay for decontamination of water containing Pb(II), Cu(II), Zn(II) and pesticides. Separation science and technology (Print), 52(1), 58-70
Open this publication in new window or tab >>Composite nanofibers of polyacrylonitrile/natural clay for decontamination of water containing Pb(II), Cu(II), Zn(II) and pesticides
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2017 (English)In: Separation science and technology (Print), ISSN 0149-6395, E-ISSN 1520-5754, Vol. 52, no 1, p. 58-70Article in journal (Refereed) Published
Abstract [en]

Composite nanofibers containing polyacrylonitrile and natural clay particles were fabricated and investigated for the removal of Pb(II), Cu(II) and Zn(II) from aqueous solutions. The adsorption behavior of Pb(II), Cu(II) and Zn(II) can be well described by the Langmuir adsorption model and high loading capacities at pH 7 were obtained. The kinetics of the adsorption process showed that equilibrium was attained after 60 min and the experimental data followed a pseudo-first-order model. The nanocomposites were also tested for photocatalytic degradation of Monocrotophos pesticides in which high degradation efficiency (>90%) was obtained in less than 60 min.

Place, publisher, year, edition, pages
Taylor & Francis, 2017
Keywords
Composite nanofibers, heavy metals, pesticides, polluted waters, Degradation, Lead, Nanofibers, Solutions, Water pollution, Zinc, Zinc compounds, Adsorption behavior, Adsorption process, Degradation efficiency, Langmuir adsorption model, Photo catalytic degradation, Polluted water, Pseudo-first-order, Adsorption
National Category
Nano Technology
Identifiers
urn:nbn:se:kth:diva-202238 (URN)10.1080/01496395.2016.1231692 (DOI)000392446300007 ()2-s2.0-84991498328 (Scopus ID)
Note

Correspondence Address: Uheida, A.; Department of Materials and Nano Physics, KTH-Royal Institute of Technology, Electrum 229 - Isafjordsgatan 22, Sweden; email: salam@kth.se. QC 20170320

Available from: 2017-03-20 Created: 2017-03-20 Last updated: 2024-03-15Bibliographically approved
Toprak, M., Li, S. & Muhammed, M. (2017). Fabrication routes for nanostructured TE material architectures. In: Materials, Preparation, and Characterization in Thermoelectrics: (pp. 17-1-17-18). CRC Press
Open this publication in new window or tab >>Fabrication routes for nanostructured TE material architectures
2017 (English)In: Materials, Preparation, and Characterization in Thermoelectrics, CRC Press , 2017, p. 17-1-17-18Chapter in book (Other academic)
Abstract [en]

Nanomaterials have been an emerging œeld of research due to the novel properties exhibited when the size of building blocks is reduced below 100 nm. Several size-dependent phenomena make nanomaterials attractive in terms of potential applicability compared to their larger-sized counterparts, justifying the importance and attention of this research.1-3 For thermoelectric research, nanomaterials are of great interest due to the possibility of decoupling electrical and thermal transport properties which may help attain higher ZT values for the currently available materials.4,5 še commonly accepted nomenclature of nanomaterials is based on the number of degrees of freedom of charge carriers for the description of their dimensionalities. šus, a thin œlm or superlattice is conœned in one dimension, but is a 2D nanomaterial. Similarly, a nanowire is conœned in two dimensions, hence is a 1D nanomaterial, and œnally a nanoparticle is conœned in three dimensions and is therefore a 0D nanomaterial. In the case of larger nanocrystals (not quantum conœned), the size and shape of the nanocrystals also affect their properties due to differences in surface-to-volume ratio. Bulk nanostructured (NS) TE materials are fabricated using a bulk process rather than a nanofabrication process, which has the important advantage of being produced in large quantities and in a form that is compatible with commercially available devices.6 šey are different from the advanced low-dimensional TE materials as they are consolidated under high T and P, resulting in larger-sized (40-200 nm) grains. še advanced low-dimensional TE materials are usually in quantum size and have well-deœned shapes such as rod-like or tube-like structures. še nanoeffects of bulk NS TE materials will not be as strong as in the case of advanced low-dimensional TE materials, but since the material is closer to bulk material, it can be easily handled the same way as bulk TE materials using conventional TE module/device technology for further steps toward direct applications. So far, of all the NS materials, only bulk NS materials have been produced in enough quantity to be used in this manner. 

Place, publisher, year, edition, pages
CRC Press, 2017
National Category
Materials Engineering
Identifiers
urn:nbn:se:kth:diva-236811 (URN)10.1201/b11891 (DOI)2-s2.0-85051958883 (Scopus ID)
Note

Duplicate record in Scopus 2-s2.0-85139642938

QC 20230608

Available from: 2019-01-07 Created: 2019-01-07 Last updated: 2023-06-08Bibliographically approved
El-Sayed, R., Ye, F., Asem, H., Ashour, R., Zheng, W., Muhammed, M. & Hassan, M. (2017). Importance of the surface chemistry of nanoparticles on peroxidase-like activity. Biochemical and Biophysical Research Communications - BBRC, 491(1), 15-18
Open this publication in new window or tab >>Importance of the surface chemistry of nanoparticles on peroxidase-like activity
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2017 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 491, no 1, p. 15-18Article in journal (Refereed) Published
Abstract [en]

We report the studies on origin of peroxidase-like activity for gold nanoparticles, as well as the impact from morphology and surface charge of nanoparticles. For this purpose, we have synthesized hollow gold nanospheres (HAuNS) and gold nanorods (AuNR) with different morphology and surface chemistry to investigate their influence on the catalytic activity. We found that citrate-capped HAuNS show catalyzing efficiency in oxidation reaction of 3,3′,5,5′-tetramethylbenzidine (TMB) by hydrogen peroxide (H2O2) and it is superior to that of cetyltrimethylammonium bromide (CTAB)-capped AuNR. The kinetics of catalytic activities from HAuNS and AuNR were respectively studied under varied temperatures. The results indicated that surface chemistry rather than morphology of nanoparticles plays an important role in the catalytic reaction of substrate. Furthermore, influencing factors such as pH, amount of nanoparticle and H2O2 concentration were also investigated on HAuNS-catalyzed system. The great impact of nanoparticle surface properties on catalytic reactions makes a paradigm in constructing nanozymes as peroxidase mimic for sensing application.

Place, publisher, year, edition, pages
Elsevier, 2017
Keywords
Enzyme mimic, Gold nanorods, Hollow gold nanospheres, Peroxidase activity, Surface chemistry
National Category
Materials Chemistry
Identifiers
urn:nbn:se:kth:diva-212231 (URN)10.1016/j.bbrc.2017.07.014 (DOI)000408518700003 ()28687493 (PubMedID)2-s2.0-85023636388 (Scopus ID)
Funder
Swedish Cancer Society, CAN 2014/759
Note

QC 20170821

Available from: 2017-08-21 Created: 2017-08-21 Last updated: 2024-03-18Bibliographically approved
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