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Björlenius, Berndt
Publications (10 of 10) Show all publications
Ostman, M., Björlenius, B., Fick, J. & Tysklind, M. (2019). Effect of full-scale ozonation and pilot-scale granular activated carbon on the removal of biocides, antimycotics and antibiotics in a sewage treatment plant. Science of the Total Environment, 649, 1117-1123
Open this publication in new window or tab >>Effect of full-scale ozonation and pilot-scale granular activated carbon on the removal of biocides, antimycotics and antibiotics in a sewage treatment plant
2019 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 649, p. 1117-1123Article in journal (Refereed) Published
Abstract [en]

Several micropollutants show low removal efficiencies in conventional sewage treatment plants, and therefore enter the aquatic environment. To reduce the levels of micropollutants in sewage effluent, and thereby the effects on biota, a number of extra treatment steps are currently being evaluated. Two such techniques are ozonation and adsorption onto activated carbon. In this study, we investigated the efficiency of Sweden's first full-scale ozonation treatment plant at removing a number of antibiotics, antimycotics and biocides. The effect of adding granular activated carbon (GAC) on a pilot scale and pilot-scale ozonation were also evaluated. The conventional treatment (13,000 PE) with the add-on of full-scale ozonation (0.55 g O-3/g Total organic carbon (TOC)) was able to remove most of the studied compounds (>90%), except for benzotriazoles and fluconazole (<50%). Adsorption on GAC on a pilot scale showed a higher removal efficiency than ozonation (>80% for all studied compounds). Three types of GAC were evaluated and shown to have different removal efficiencies. In particular, the GAC with the smallest particle sizes exhibited the highest removal efficiency. The results demonstrate that it is important to select an appropriate type of carbon to achieve the removal goal for specific target compounds.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Biocides, Antibiotics, Ozonation, GAC, Removal efficiency, Wastewater
National Category
Environmental Sciences
Identifiers
urn:nbn:se:kth:diva-237079 (URN)10.1016/j.scitotenv.2018.08.382 (DOI)000446076500106 ()30308883 (PubMedID)2-s2.0-85052640940 (Scopus ID)
Funder
Mistra - The Swedish Foundation for Strategic Environmental ResearchSwedish Research Council FormasSwedish Energy Agency
Note

QC 20181024

Available from: 2018-10-24 Created: 2018-10-24 Last updated: 2018-10-24Bibliographically approved
Östman, M., Björlenius, B., Fick, J. & Tysklind, M. (2019). Effect of full-scale ozonation and pilot-scale granular activated carbon on the removal of biocides, antimycotics and antibiotics in a sewage treatment plant. Science of the Total Environment, 649, 1117-1123
Open this publication in new window or tab >>Effect of full-scale ozonation and pilot-scale granular activated carbon on the removal of biocides, antimycotics and antibiotics in a sewage treatment plant
2019 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 649, p. 1117-1123Article in journal (Refereed) Published
Abstract [en]

Several micropollutants show low removal efficiencies in conventional sewage treatment plants, and therefore enter the aquatic environment. To reduce the levels of micropollutants in sewage effluent, and thereby the effects on biota, a number of extra treatment steps are currently being evaluated. Two such techniques are ozonation and adsorption onto activated carbon. In this study, we investigated the efficiency of Sweden's first full-scale ozonation treatment plant at removing a number of antibiotics, antimycotics and biocides. The effect of adding granular activated carbon (GAC) on a pilot scale and pilot-scale ozonation were also evaluated. The conventional treatment (13,000 PE) with the add-on of full-scale ozonation (0.55 g O3/g Total organic carbon (TOC)) was able to remove most of the studied compounds (&gt;90%), except for benzotriazoles and fluconazole (&lt;50%). Adsorption on GAC on a pilot scale showed a higher removal efficiency than ozonation (&gt;80% for all studied compounds). Three types of GAC were evaluated and shown to have different removal efficiencies. In particular, the GAC with the smallest particle sizes exhibited the highest removal efficiency. The results demonstrate that it is important to select an appropriate type of carbon to achieve the removal goal for specific target compounds.

Place, publisher, year, edition, pages
Elsevier, 2019
Keywords
Antibiotics, Biocides, GAC, Ozonation, Removal efficiency, Wastewater, Activated carbon, Activated carbon treatment, Efficiency, Effluent treatment, Effluents, Granular materials, Organic carbon, Ozone, Ozone water treatment, Ozonization, Removal, River pollution, Aquatic environments, Conventional treatments, Granular activated carbons, Low removal efficiencies, Ozonation treatment, Removal efficiencies, Sewage effluents, Total Organic Carbon, Sewage treatment plants, 2 (methylthio)benzothiazole, antibiotic agent, antifungal agent, benzothiazole derivative, benzotriazole derivative, biocide, ciprofloxacin, econazole, erythromycin, fluconazole, fluorine, granular activated carbon, metronidazole, terbinafine, trimethoprim, unclassified drug, effluent, pesticide, pollutant removal, sewage, wastewater treatment plant, adsorption kinetics, Article, biofilm, biological activity, concentration (parameters), controlled study, electrophilicity, filtration, limit of quantitation, particle size, priority journal, sewage treatment plant, waste component removal, Sweden
National Category
Environmental Biotechnology
Identifiers
urn:nbn:se:kth:diva-236327 (URN)10.1016/j.scitotenv.2018.08.382 (DOI)000446076500106 ()2-s2.0-85052640940 (Scopus ID)
Funder
Swedish Research Council FormasMistra - The Swedish Foundation for Strategic Environmental ResearchSwedish Agency for Marine and Water Management
Note

QC 20181120

Available from: 2018-11-20 Created: 2018-11-20 Last updated: 2018-11-20Bibliographically approved
Wang, H., Sikora, P., Rutgersson, C., Lindh, M., Brodin, T., Björlenius, B., . . . Norder, H. (2018). Differential removal of human pathogenic viruses from sewage by conventional and ozone treatments. International journal of hygiene and environmental health (Print), 221(3), 479-488
Open this publication in new window or tab >>Differential removal of human pathogenic viruses from sewage by conventional and ozone treatments
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2018 (English)In: International journal of hygiene and environmental health (Print), ISSN 1438-4639, E-ISSN 1618-131X, Vol. 221, no 3, p. 479-488Article in journal (Refereed) Published
Abstract [en]

Sewage contains a mixed ecosystem of diverse sets of microorganisms, including human pathogenic viruses. Little is known about how conventional as well as advanced treatments of sewage, such as ozonation, reduce the environmental spread of viruses. Analyses for viruses were therefore conducted for three weeks in influent, after conventional treatment, after additional ozonation, and after passing an open dam system at a full-scale treatment plant in Knivsta, Sweden. Viruses were concentrated by adsorption to a positively charged filter, from which they were eluted and pelleted by ultracentrifugation, with a recovery of about 10%. Ion Torrent sequencing was used to analyze influent, leading to the identification of at least 327 viral species, most of which belonged to 25 families with some having unclear classification. Real-time PCR was used to test for 21 human-related viruses in inlet, conventionally treated, and ozone-treated sewage and outlet waters. The viruses identified in influent and further analyzed were adenovirus, norovirus, sapovirus, parechovirus, hepatitis E virus, astrovirus, pecovirus, picobirnavirus, parvovirus, and gokushovirus. Conventional treatment reduced viral concentrations by one to four log10, with the exception of adenovirus and parvovirus, for which the removal was less efficient. Ozone treatment led to a further reduction by one to two log10, but less for adenovirus. This study showed that the amount of all viruses was reduced by conventional sewage treatment. Further ozonation reduced the amounts of several viruses to undetectable levels, indicating that this is a promising technique for reducing the transmission of many pathogenic human viruses. 

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Adenovirus, Effluent, Next generation sequencing, Parvovirus, Waste water
National Category
Earth and Related Environmental Sciences
Identifiers
urn:nbn:se:kth:diva-227368 (URN)10.1016/j.ijheh.2018.01.012 (DOI)000438327900012 ()29402695 (PubMedID)2-s2.0-85041284211 (Scopus ID)
Funder
Swedish Research Council Formas, 942-2015-306
Note

QC 20180727

Available from: 2018-06-04 Created: 2018-06-04 Last updated: 2018-07-27Bibliographically approved
Pohl, J., Björlenius, B., Brodin, T., Carlsson, G., Fick, J., Larsson, D. G., . . . Örn, S. (2018). Effects of ozonated sewage effluent on reproduction and behavioral endpoints in zebrafish (Danio rerio). Aquatic Toxicology, 200, 93-101
Open this publication in new window or tab >>Effects of ozonated sewage effluent on reproduction and behavioral endpoints in zebrafish (Danio rerio)
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2018 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 200, p. 93-101Article in journal (Refereed) Published
Abstract [en]

Pharmaceutical residues and other micro-contaminants may enter aquatic environments through effluent from sewage treatment plants (STPs) and could cause adverse effects in wild fish. One strategy to alleviate this situation is to improve wastewater treatment by ozonation. To test the effectiveness of full-scale wastewater effluent ozonation at a Swedish municipal STP, the added removal efficiency was measured for 105 pharmaceuticals. In addition, gene expression, reproductive and behavioral endpoints were analyzed in zebrafish (Danio rerio) exposed on-site over 21 days to ozonated or non-ozonated effluents as well as to tap water. Ozone treatment (7 g O3/m3) removed pharmaceuticals by an average efficiency of 77% in addition to the conventional treatment, leaving 11 screened pharmaceuticals above detection limits. Differences in biological responses of the exposure treatments were recorded in gene expression, reproduction and behavior. Hepatic vitellogenin gene expression was higher in male zebrafish exposed to the ozonated effluent compared to the non-ozonated effluent and tap water treatments. The reproductive success was higher in fish exposed to ozonated effluent compared to non-ozonated effluent and to tap water. The behavioral measurements showed that fish exposed to the ozonated STP effluent were less active in swimming the first minute after placed in a novel vessel. Ozonation is a capable method for removing pharmaceuticals in effluents. However, its implementation should be thoroughly evaluated for any potential biological impact. Future research is needed for uncovering the factors which produced the in vivo responses in fish.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Fecundity, Ozone, Pharmaceuticals, Vitellogenin, Wastewater
National Category
Other Biological Topics
Identifiers
urn:nbn:se:kth:diva-228731 (URN)10.1016/j.aquatox.2018.04.014 (DOI)000438180700010 ()29729477 (PubMedID)2-s2.0-85046783086 (Scopus ID)
Funder
Mistra - The Swedish Foundation for Strategic Environmental ResearchSwedish Agency for Marine and Water Management
Note

QC 20180529

Available from: 2018-05-29 Created: 2018-05-29 Last updated: 2018-08-03Bibliographically approved
Björlenius, B., Ripszám, M., Haglund, P., Lindberg, R. H., Tysklind, M. & Fick, J. (2018). Pharmaceutical residues are widespread in Baltic Sea coastal and offshore waters – Screening for pharmaceuticals and modelling of environmental concentrations of carbamazepine. Science of the Total Environment, 633, 1496-1509
Open this publication in new window or tab >>Pharmaceutical residues are widespread in Baltic Sea coastal and offshore waters – Screening for pharmaceuticals and modelling of environmental concentrations of carbamazepine
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2018 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 633, p. 1496-1509Article in journal (Refereed) Published
Abstract [en]

The consumption of pharmaceuticals worldwide coupled with modest removal efficiencies of sewage treatment plants have resulted in the presence of pharmaceuticals in aquatic systems globally. In this study, we investigated the environmental concentrations of a selection of 93 pharmaceuticals in 43 locations in the Baltic Sea and Skagerrak. The Baltic Sea is vulnerable to anthropogenic activities due to a long turnover time and a sensitive ecosystem in the brackish water. Thirty-nine of 93 pharmaceuticals were detected in at least one sample, with concentrations ranging between 0.01 and 80 ng/L. One of the pharmaceuticals investigated, the anti-epileptic drug carbamazepine, was widespread in coastal and offshore seawaters (present in 37 of 43 samples). In order to predict concentrations of pharmaceuticals in the sub-basins of the Baltic Sea, a mass balance-based grey box model was set up and the persistent, widely used carbamazepine was selected as the model substance. The model was based on hydrological and meteorological sub-basin characteristics, removal data from smaller watersheds and wastewater treatment plants, and statistics relating to population, consumption and excretion rate of carbamazepine in humans. The grey box model predicted average environmental concentrations of carbamazepine in sub-basins with no significant difference from the measured concentrations, amounting to 0.57-3.2 ng/L depending on sub-basin location. In the Baltic Sea, the removal rate of carbamazepine in seawater was estimated to be 6.2 10(-9) s(-1) based on a calculated half-life time of 3.5 years at 10 degrees C, which demonstrates the long response time of the environment to measures phasing out persistent or slowly degradable substances such as carbamazepine. Sampling, analysis and grey box modelling were all valuable in describing the presence and removal of carbamazepine in the Baltic Sea.

Place, publisher, year, edition, pages
Elsevier, 2018
Keywords
Baltic Sea, Carbamazepine, Coastal and offshore waters, Half-life time, Model, Pharmaceuticals
National Category
Environmental Sciences
Identifiers
urn:nbn:se:kth:diva-227511 (URN)10.1016/j.scitotenv.2018.03.276 (DOI)000432475300145 ()29758901 (PubMedID)2-s2.0-85044791102 (Scopus ID)
Funder
Mistra - The Swedish Foundation for Strategic Environmental Research
Note

QC 20180516

Available from: 2018-05-16 Created: 2018-05-16 Last updated: 2018-11-26Bibliographically approved
Björlenius, B. (2018). Pharmaceuticals – improved removal from municipal wastewater and their occurrence in the Baltic Sea. (Doctoral dissertation). Stockholm: KTH Royal Institute of Technology
Open this publication in new window or tab >>Pharmaceuticals – improved removal from municipal wastewater and their occurrence in the Baltic Sea
2018 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Pharmaceutical residues are found in the environment due to extensive use in human and veterinary medicine. The active pharmaceutical ingredients (APIs) have a potential impact in non-target organisms. Municipal wastewater treatment plants (WWTPs) are not designed to remove APIs.

In this thesis, two related matters are addressed 1) evaluation of advanced treatment to remove APIs from municipal wastewater and 2) the prevalence and degradation of APIs in the Baltic Sea.

A stationary pilot plant with nanofiltration (NF) and a mobile pilot plant with activated carbon and ozonation were designed to study the removal of APIs at four WWTPs. By NF, removal reached 90%, but the retentate needed further treatment. A predictive model of the rejection of APIs by NF was developed based on the variables: polarizability, globularity, ratio hydrophobic to polar water accessible surface and charge. The pilot plants with granular and powdered activated carbon (GAC) and (PAC) removed more than 95% of the APIs. Screening of activated carbon products was essential, because of a broad variation in adsorption capacity. Recirculation of PAC or longer contact time, increased the removal of APIs. Ozonation with 5-7 g/m3 ozone resulted in 87-95% removal of APIs. Elevated activity and transcription of biomarkers indicated presence of xenobiotics in regular effluent. Chemical analysis of APIs, together with analysis of biomarkers, were valuable and showed that GAC-filtration and ozonation can be implemented to remove APIs in WWTPs, with decreased biomarker responses.

Sampling of the Baltic Sea showed presence of APIs in 41 out of 43 locations. A developed grey box model predicted concentration and half-life of carbamazepine in the Baltic Sea to be 1.8 ng/L and 1300 d respectively.

In conclusion, APIs were removed to 95% by GAC or PAC treatment. The additional treatment resulted in lower biomarker responses than today and some APIs were shown to be widespread in the aquatic environment.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2018. p. 150
Series
TRITA-CBH-FOU ; 2018-62
Keywords
Advanced wastewater treatment, WWTP, pilot plant, pharmaceutical residues, removal of pharmaceuticals, activated carbon, ozonation, nanofiltration, biomarker, Baltic Sea
National Category
Bioprocess Technology
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-239307 (URN)978-91-7873-047-6 (ISBN)
Public defence
2018-12-20, F3, Lindstedtsvägen 26, Stockholm, 10:00 (English)
Opponent
Supervisors
Funder
Mistra - The Swedish Foundation for Strategic Environmental Research, MistraPharma
Note

QC 20181120

Available from: 2018-11-20 Created: 2018-11-20 Last updated: 2018-11-20Bibliographically approved
Kårelid, V., Larsson, G. & Björlenius, B. (2017). Effects of recirculation in a three-tank pilot-scale system for pharmaceutical removal with powdered activated carbon. Journal of Environmental Management, 193(May), 163-Environmental Impact Optimization of Reinforced Concrete Slab Frame Bridges
Open this publication in new window or tab >>Effects of recirculation in a three-tank pilot-scale system for pharmaceutical removal with powdered activated carbon
2017 (English)In: Journal of Environmental Management, ISSN 0301-4797, E-ISSN 1095-8630, Vol. 193, no May, p. 163-Environmental Impact Optimization of Reinforced Concrete Slab Frame BridgesArticle, review/survey (Refereed) Accepted
Abstract [en]

The removal of pharmaceutically active compounds by powdered activated carbon (PAC) in municipal wastewater is a promising solution to the problem of polluted recipient waters. Today, an efficient design strategy is however lacking with regard to high-level overall, and specific, substance removal in the large scale. The performance of PAC-based removal of pharmaceuticals was studied in pilot-scale with respect to the critical parameters; contact time and PAC dose using one PAC product selected by screening in bench-scale. The goal was a minimum of 95% removal of the pharmaceuticals present in the evaluated municipal wastewater. A set of 21 pharmaceuticals was selected from an initial 100 due to their high occurrence in the effluent water of two selected wastewater treatment plants (WWTPs) in Sweden, whereof candidates discussed for future EU regulation directives were included. By using recirculation of PAC over a treatment system using three sequential contact tanks, a combination of the benefits of powdered and granular carbon performance was achieved. The treatment system was designed so that recirculation could be introduced to any of the three tanks to investigate the effect of recirculation on the adsorption performance. This was compared to use of the setup, but without recirculation. A higher degree of pharmaceutical removal was achieved in all recirculation setups, both overall and with respect to specific substances, as compared to without recirculation. Recirculation was tested with nominal contact times of 30, 60 and 120 min and the goal of 95% removal could be achieved already at the shortest contact times at a PAC dose of 10–15 mg/L. In particular, the overall removal could be increased even to 97% and 99%, at 60 and 120 min, respectively, when the recirculation point was the first tank. Recirculation of PAC to either the first or the second contact tank proved to be comparable, while a slightly lower performance was observed with recirculation to the third tank. With regards to individual substances, clarithromycin and diclofenac were ubiquitously removed according to the set goal and in contrast, a few substances (fluconazole, irbesartan, memantine and venlafaxine) required specific settings to reach an acceptable removal.

Keywords
Adsorption, Advanced wastewater treatment, Municipal wastewater, PAC, Pharmaceuticals, Recirculation
National Category
Water Treatment
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-195707 (URN)10.1016/j.jenvman.2017.01.078 (DOI)000397687100018 ()2-s2.0-85013040903 (Scopus ID)
Projects
Mistra Pharma
Note

QC 20170314

Available from: 2016-11-08 Created: 2016-11-08 Last updated: 2018-11-26Bibliographically approved
Kårelid, V., Larsson, G. & Björlenius, B. (2017). Pilot-scale removal of pharmaceuticals in municipal wastewater: Comparison of granular and powdered activated carbon treatment at three wastewater treatment plants. Journal of Environmental Management, 193(-1), 491-502
Open this publication in new window or tab >>Pilot-scale removal of pharmaceuticals in municipal wastewater: Comparison of granular and powdered activated carbon treatment at three wastewater treatment plants
2017 (English)In: Journal of Environmental Management, ISSN 0301-4797, E-ISSN 1095-8630, Vol. 193, no -1, p. 491-502Article in journal (Refereed) Published
Abstract [en]

Adsorption with activated carbon is widely suggested as an option for the removal of organic micropollutants including pharmaceutically active compounds (PhACs) in wastewater. In this study adsorption with granular activated carbon (GAC) and powdered activated carbon (PAC) was analyzed and compared in parallel operation at three Swedish wastewater treatment plants with the goal to achieve a 95% PhAC removal. Initially, mapping of the prevalence of over 100 substances was performed at each plant and due to low concentrations a final 22 were selected for further evaluation. These include carbamazepine, clarithromycin and diclofenac, which currently are discussed for regulation internationally. A number of commercially available activated carbon products were initially screened using effluent wastewater. Of these, a reduced set was selected based on adsorption characteristics and cost. Experiments designed with the selected carbons in pilot-scale showed that most products could indeed remove PhACs to the target level, both on total and individual basis. In a setup using internal recirculation the PAC system achieved a 95% removal applying a fresh dose of 15–20 mg/L, while carbon usage rates for the GAC application were much broader and ranged from <28 to 230 mg/L depending on the carbon product. The performance of the PAC products generally gave better results for individual PhACs in regards to carbon availability. All carbon products showed a specific adsorption for a specific PhAC meaning that knowledge of the target pollutants must be acquired before successful design of a treatment system. In spite of different configurations and operating conditions of the different wastewater treatment plants no considerable differences regarding pharmaceutical removal were observed.

Place, publisher, year, edition, pages
Academic Press, 2017
Keywords
Adsorption, Advanced wastewater treatment, Municipal wastewater, PAC, Pharmaceuticals, Recirculation
National Category
Water Treatment
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-195703 (URN)10.1016/j.jenvman.2017.02.042 (DOI)000397687100049 ()2-s2.0-85014089196 (Scopus ID)
Projects
Mistra Pharma
Note

QC 20161124

Available from: 2016-11-08 Created: 2016-11-08 Last updated: 2018-11-26Bibliographically approved
Beijer, K., Björlenius, B., Shaik, S., Lindberg, R. H., Brunström, B. & Brandt, I. (2017). Removal of pharmaceuticals and unspecified contaminants in sewage treatment effluents by activated carbon filtration and ozonation: Evaluation using biomarker responses and chemical analysis. Chemosphere, 176, 342-351
Open this publication in new window or tab >>Removal of pharmaceuticals and unspecified contaminants in sewage treatment effluents by activated carbon filtration and ozonation: Evaluation using biomarker responses and chemical analysis
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2017 (English)In: Chemosphere, ISSN 0045-6535, E-ISSN 1879-1298, Vol. 176, p. 342-351Article in journal (Refereed) Published
Abstract [en]

Traces of active pharmaceutical ingredients (APIs) and other chemicals are demonstrated in effluents from sewage treatment plants (STPs) and they may affect quality of surface water and eventually drinking water. Treatment of effluents with granular activated carbon (GAC) or ozone to improve removal of APIs and other contaminants was evaluated at two Swedish STPs, Käppala and Uppsala (88 and 103 APIs analyzed). Biomarker responses in rainbow trout exposed to regular and additionally treated effluents were determined. GAC and ozone treatment removed 87–95% of the total concentrations of APIs detected. In Käppala, GAC removed 20 and ozonation (7 g O3/m3) 21 of 24 APIs detected in regular effluent. In Uppsala, GAC removed 25 and ozonation (5.4 g O3/m3) 15 of 25 APIs detected in effluent. GAC and ozonation also reduced biomarker responses caused by unidentified pollutants in STP effluent water. Elevated ethoxyresorufin-O-deethylase (EROD) activity in gills was observed in fish exposed to effluent in both STPs. Gene expression analysis carried out in Käppala showed increased concentrations of cytochrome P450 (CYP1As and CYP1C3) transcripts in gills and of CYP1As in liver of fish exposed to effluent. In fish exposed to GAC- or ozone-treated effluent water, gill EROD activity and expression of CYP1As and CYP1C3 in gills and liver were generally equal to or below levels in fish held in tap water. The joint application of chemical analysis and sensitive biomarkers proved useful for evaluating contaminant removal in STPs with new technologies.

Place, publisher, year, edition, pages
Elsevier Ltd, 2017
Keywords
Activated carbon, Biomarkers, Ozonation, Pharmaceuticals, Rainbow trout, Wastewater
National Category
Electrical Engineering, Electronic Engineering, Information Engineering
Identifiers
urn:nbn:se:kth:diva-207305 (URN)10.1016/j.chemosphere.2017.02.127 (DOI)000399849300039 ()2-s2.0-85014414522 (Scopus ID)
Note

QC 20170613

Available from: 2017-06-13 Created: 2017-06-13 Last updated: 2018-11-26Bibliographically approved
Bengtsson-Palme, J., Hammaren, R., Pal, C., Ostman, M., Björlenius, B., Flach, C.-F., . . . Larsson, D. G. (2016). Elucidating selection processes for antibiotic resisitance in sewage treatment plants using metagenomics. Science of the Total Environment, 572, 697-712
Open this publication in new window or tab >>Elucidating selection processes for antibiotic resisitance in sewage treatment plants using metagenomics
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2016 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 572, p. 697-712Article in journal (Refereed) Published
Abstract [en]

Sewage treatment plants (STPs) have repeatedly been suggested as hotspots for the emergence and dissemination of antibiotic-resistant bacteria. A critical question still unanswered is if selection pressures within STPs, caused by residual antibiotics or other co-selective agents, are sufficient to specifically promote resistance. To address this, we employed shotgun metagenomic sequencing of samples from different steps of the treatment process in three Swedish STPs. In parallel, concentrations of selected antibiotics, biocides and metals were analyzed. We found that concentrations of tetracycline and ciprofloxacin in the influent were above predicted concentrations for resistance selection, however, there was no consistent enrichment of resistance genes to any particular class of antibiotics in the STPs, neither for biocide and metal resistance genes. The most substantial change of the bacterial communities compared to human feces occurred already in the sewage pipes, manifested by a strong shift from obligate to facultative anaerobes. Through the treatment process, resistance genes against antibiotics, biocides and metals were not reduced to the same extent as fecal bacteria. The OXA-48 gene was consistently enriched in surplus and digested sludge. We find this worrying as OXA-48, still rare in Swedish clinical isolates, provides resistance to carbapenems, one of our most critically important classes of antibiotics. Taken together, metagenomics analyses did not provide clear support for specific antibiotic resistance selection. However, stronger selective forces affecting gross taxonomic composition, and with that resistance gene abundances, limit interpretability. Comprehensive analyses of resistant/non-resistant strains within relevant species are therefore warranted. 

Place, publisher, year, edition, pages
Elsevier, 2016
Keywords
Antibiotic resistance genes, Co-selection, Fecal bacteria, Microbial ecology, Risk assessment, Wastewater treatment
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-197765 (URN)10.1016/j.scitotenv.2016.06.228 (DOI)000387807200068 ()27542633 (PubMedID)2-s2.0-84989282570 (Scopus ID)
Note

QC 20161229

Available from: 2016-12-29 Created: 2016-12-08 Last updated: 2017-11-29Bibliographically approved
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