Change search
Link to record
Permanent link

Direct link
BETA
Svahn Andersson, Helene
Publications (10 of 12) Show all publications
Reu, P., Svedberg, G., Hässler, L., Möller, B., Svahn Andersson, H. & Gantelius, J. (2019). A 61% lighter cell culture dish to reduce plastic waste. PLoS ONE, 14(4), Article ID e0216251.
Open this publication in new window or tab >>A 61% lighter cell culture dish to reduce plastic waste
Show others...
2019 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 14, no 4, article id e0216251Article in journal (Refereed) Published
Abstract [en]

Cell culture is a ubiquitous and flexible research method. However, it heavily relies on plastic consumables generating millions of tonnes of plastic waste yearly. Plastic waste is a major and growing global concern. Here we describe a new cell culture dish that offers a culture area equivalent to three petri dishes but that is on average 61% lighter and occupies 67% less volume. Our dish is composed of a lid and three thin containers surrounded by a light outer shell. Cell culture can be performed in each of the containers sequentially. The outer shell provides the appropriate structure for the manipulation of the dish as a whole. The prototype was tested by sequentially growing cells in each of its containers. As a control, sequential cultures in groups of 3 petri dishes were performed. No statistical differences were found between the prototype and the control in terms of cell number, cell viability or cell distribution.

Place, publisher, year, edition, pages
Public Library of Science, 2019
National Category
Environmental Engineering
Identifiers
urn:nbn:se:kth:diva-258015 (URN)10.1371/journal.pone.0216251 (DOI)000466364800040 ()31039189 (PubMedID)2-s2.0-85065404122 (Scopus ID)
Note

QC 20191004

Available from: 2019-09-09 Created: 2019-09-09 Last updated: 2019-10-07Bibliographically approved
Nybond, S., Réu, P., Rhedin, S., Svedberg, G., Alfvén, T., Gantelius, J. & Svahn Andersson, H. (2019). Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray.. Analytical and Bioanalytical Chemistry, 411(4), 813-822
Open this publication in new window or tab >>Adenoviral detection by recombinase polymerase amplification and vertical flow paper microarray.
Show others...
2019 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 411, no 4, p. 813-822Article in journal (Refereed) Published
Abstract [en]

Respiratory viral infections often mimic the symptoms of infections caused by bacteria; however, restricted and targeted administration of antibiotics is needed to combat growing antimicrobial resistance. This is particularly relevant in low-income settings. In this work, we describe the use of isothermal amplification of viral DNA at 37 °C coupled to a paper-based vertical flow microarray (VFM) setup that utilizes a colorimetric detection of amplicons using functionalized gold nanoparticles. Two oligonucleotide probes, one in-house designed and one known adenoviral probe were tested and validated for microarray detection down to 50 nM using a synthetic target template. Furthermore, primers were shown to function in a recombinase polymerase amplification reaction using both synthetic template and viral DNA. As a proof-of-concept, we demonstrate adenoviral detection with four different adenoviral species associated with respiratory infections using the paper-based VFM format. The presented assay was validated with selected adenoviral species using the in-house probe, enabling detection at 1 ng of starting material with intra- and inter-assay %CV of ≤ 9% and ≤ 13%. Finally, we validate our overall method using clinical samples. Based on the results, the combination of recombinase polymerase amplification, paper microarray analysis, and nanoparticle-based colorimetric detection could thus be a useful strategy towards rapid and affordable multiplexed viral diagnostics.

Place, publisher, year, edition, pages
Springer, 2019
Keywords
Adenoviral, RPA, VFM
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-244426 (URN)10.1007/s00216-018-1503-y (DOI)000456132900003 ()30498984 (PubMedID)2-s2.0-8505759966 (Scopus ID)
Note

QC 20190222

Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-22Bibliographically approved
Antypas, H., Veses-Garcia, M., Weibull, E., Svahn Andersson, H. & Richter-Dahlfors, A. (2018). A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide. Lab on a Chip, 18(12), 1767-1777
Open this publication in new window or tab >>A universal platform for selection and high-resolution phenotypic screening of bacterial mutants using the nanowell slide
Show others...
2018 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 18, no 12, p. 1767-1777Article in journal (Refereed) Published
Abstract [en]

The Petri dish and microtiter plate are the golden standard for selection and screening of bacteria in microbiological research. To improve on the limited resolution and throughput of these methods, we developed a universal, user-friendly platform for selection and high-resolution phenotypic screening based on the nanowell slide. This miniaturized platform has an optimal ratio between throughput and assay complexity, holding 672 nanowells of 500 nl each. As monoclonality is essential in bacterial genetics, we used FACS to inoculate each nanowell with a single bacterium in 15 min. We further extended the protocol to select and sort only bacteria of interest from a mixed culture. We demonstrated this by isolating single transposon mutants generated by a custom-made transposon with dual selection for GFP fluorescence and kanamycin resistance. Optical compatibility of the nanowell slide enabled phenotypic screening of sorted mutants by spectrophotometric recording during incubation. By processing the absorbance data with our custom algorithm, a phenotypic screen for growth-associated mutations was performed. Alternatively, by processing fluorescence data, we detected metabolism-associated mutations, exemplified by a screen for -galactosidase activity. Besides spectrophotometry, optical compatibility enabled us to perform microscopic analysis directly in the nanowells to screen for mutants with altered morphologies. Despite the miniaturized format, easy transition from nano- to macroscale cultures allowed retrieval of bacterial mutants for downstream genetic analysis, demonstrated here by a cloning-free single-primer PCR protocol. Taken together, our FACS-linked nanowell slide replaces manual selection of mutants on agar plates, and enables combined selection and phenotypic screening in a one-step process. The versatility of the nanowell slide, and the modular workflow built on mainstream technologies, makes our universal platform widely applicable in microbiological research.

Place, publisher, year, edition, pages
ROYAL SOC CHEMISTRY, 2018
National Category
Microbiology
Identifiers
urn:nbn:se:kth:diva-231698 (URN)10.1039/c8lc00190a (DOI)000435115300009 ()29781496 (PubMedID)2-s2.0-85048406946 (Scopus ID)
Note

QC 20180824

Available from: 2018-08-24 Created: 2018-08-24 Last updated: 2018-08-24Bibliographically approved
Reuterswärd, P., Bergström, S., Orikiiriza, J., Lindquist, E., Bergström, S., Svahn Andersson, H., . . . Nilsson, P. (2018). Levels of human proteins in plasma associated with acute paediatric malaria. Malaria Journal, 17, Article ID 426.
Open this publication in new window or tab >>Levels of human proteins in plasma associated with acute paediatric malaria
Show others...
2018 (English)In: Malaria Journal, ISSN 1475-2875, E-ISSN 1475-2875, Vol. 17, article id 426Article in journal (Refereed) Published
Abstract [en]

Background: The intimate interaction between the pathophysiology of the human host and the biology of the Plasmodium falciparum parasite results in a wide spectrum of disease outcomes in malaria. Development of severe disease is associated with a progressively augmented imbalance in pro- and anti-inflammatory responses to high parasite loads and sequestration of parasitized erythrocytes. Although these phenomena collectively constitute common denominators for the wide variety of discrete severe malaria manifestations, the mechanistic rationales behind discrepancies in outcome are poorly understood. Exploration of the human pathophysiological response by variations in protein profiles in plasma presents an excellent opportunity to increase the understanding. This is ultimately required for better prediction, prevention and treatment of malaria, which is essential for ongoing elimination and eradication efforts. Results: An affinity proteomics approach was used to analyse 541 paediatric plasma samples collected from community controls and patients with mild or severe malaria in Rwanda. Protein profiles were generated with an antibody-based suspension bead array containing 255 antibodies targetting 115 human proteins. Here, 57 proteins were identified with significantly altered levels (adjusted p-values<0.001) in patients with malaria compared to controls. From these, the 27 most significant proteins (adjusted p-values<10(-14)) were selected for a stringent analysis approach. Here, 24 proteins showed elevated levels in malaria patients and included proteins involved in acute inflammatory response as well as cell adhesion. The remaining three proteins, also implicated in immune regulation and cellular adhesivity, displayed lower abundance in malaria patients. In addition, 37 proteins (adjusted p-values<0.05) were identified with increased levels in patients with severe compared to mild malaria. This set includes, proteins involved in tissue remodelling and erythrocyte membrane proteins. Collectively, this approach has been successfully used to identify proteins both with known and unknown association with different stages of malaria. Conclusion: In this study, a high-throughput affinity proteomics approach was used to find protein profiles in plasma linked to P. falciparum infection and malaria disease progression. The proteins presented herein are mainly involved in inflammatory response, cellular adhesion and as constituents of erythrocyte membrane. These findings have a great potential to provide increased conceptual understanding of host-parasite interaction and malaria pathogenesis.

Place, publisher, year, edition, pages
BMC, 2018
Keywords
Affinity proteomics, Human plasma profiling, Malaria, Plasmodium falciparum, suspension bead arrays, Sequestration, Cytoadhesion
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-239769 (URN)10.1186/s12936-018-2576-y (DOI)000450509700002 ()30442134 (PubMedID)2-s2.0-85056636195 (Scopus ID)
Note

QC 20190109

Available from: 2019-01-09 Created: 2019-01-09 Last updated: 2019-01-09Bibliographically approved
Dias, J. T. T., Svedberg, G., Nystrand, M., Svahn Andersson, H. & Gantelius, J. (2018). Rapid nanoprobe signal enhancement by in situ gold nanoparticle synthesis. Journal of Visualized Experiments, 2018(133), Article ID e57297.
Open this publication in new window or tab >>Rapid nanoprobe signal enhancement by in situ gold nanoparticle synthesis
Show others...
2018 (English)In: Journal of Visualized Experiments, ISSN 1940-087X, E-ISSN 1940-087X, Vol. 2018, no 133, article id e57297Article in journal (Refereed) Published
Abstract [en]

The use of nanoprobes such as gold, silver, silica or iron-oxide nanoparticles as detection reagents in bioanalytical assays can enable high sensitivity and convenient colorimetric readout. However, high densities of nanoparticles are typically needed for detection. The available synthesis-based enhancement protocols are either limited to gold and silver nanoparticles or rely on precise enzymatic control and optimization. Here, we present a protocol to enhance the colorimetric readout of gold, silver, silica, and iron oxide nanoprobes. It was observed that the colorimetric signal can be improved by up to a 10000-fold factor. The basis for such signal enhancement strategies is the chemical reduction of Au3+ to Au0. There are several chemical reactions that enable the reduction of Au3+ to Au0. In the protocol, Good's buffers and H2O2 are used and it is possible to favor the deposition of Au0 onto the surface of existing nanoprobes, in detriment of the formation of new gold nanoparticles. The protocol consists of the incubation of the microarray with a solution consisting of chloroauric acid and H2O2 in 2-(N-morpholino)ethanesulfonic acid pH 6 buffer following the nanoprobe-based detection assay. The enhancement solution can be applied to paper and glass-based sensors. Moreover, it can be used in commercially available immunoassays as demonstrated by the application of the method to a commercial allergen microarray. The signal development requires less than 5 min of incubation with the enhancement solution and the readout can be assessed by naked eye or low-end image acquisition devices such as a table-top scanner or a digital camera. 

Place, publisher, year, edition, pages
Journal of Visualized Experiments, 2018
Keywords
Glass-based assay, Gold nanoparticles, Immunoassay, Issue 133, Lateral flow assay, Microarray, Nanoprobe, Paper-based assay, Signal enhancement, Vertical flow assay
National Category
Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-227396 (URN)10.3791/57297 (DOI)000443329500046 ()29578517 (PubMedID)2-s2.0-85044726803 (Scopus ID)
Funder
EU, FP7, Seventh Framework Programme, 615458Swedish Research CouncilVINNOVAScience for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20180920

Available from: 2018-05-30 Created: 2018-05-30 Last updated: 2018-09-20Bibliographically approved
Veses-Garcia, M., Antypas, H., Loffler, S., Brauner, A., Svahn Andersson, H. & Richter-Dahlfors, A. (2018). Rapid Phenotypic Antibiotic Susceptibility Testing of Uropathogens Using Optical Signal Analysis on the Nanowell Slide. Frontiers in Microbiology, 9, Article ID 1530.
Open this publication in new window or tab >>Rapid Phenotypic Antibiotic Susceptibility Testing of Uropathogens Using Optical Signal Analysis on the Nanowell Slide
Show others...
2018 (English)In: Frontiers in Microbiology, ISSN 1664-302X, E-ISSN 1664-302X, Vol. 9, article id 1530Article in journal (Refereed) Published
Abstract [en]

Achieving fast antimicrobial susceptibility results is a primary goal in the fight against antimicrobial resistance. Standard antibiotic susceptibility testing (AST) takes, however, at least a day from patient sample to susceptibility profile. Here, we developed and clinically validated a rapid phenotypic AST based on a miniaturized nanotiter plate, the nanowell slide, that holds 672 wells in a 500 nl format for bacterial cultivation. The multitude of nanowells allows multiplexing with a panel of six antibiotics relevant for urinary tract infections. Inclusion of seven concentrations per antibiotic plus technical replicates enabled us to determine a precise minimum inhibitory concentration for 70 clinical uropathogenic Escherichia coil isolates. By combining optical recordings of bacterial growth with an algorithm for optical signal analysis, we calculated T-lag, the point of transition from lag to exponential phase, in each nanoculture. Algorithm-assisted analysis determined antibiotic susceptibility as early as 3 h 40 min. In comparison to standard disk diffusion assays, the nanowell AST showed a total categorical agreement of 97.9% with 2.6% major errors and 0% very major errors for all isolate-antibiotic combination tested. Taking advantage of the optical compatibility of the nanowell slide, we performed microscopy to illustrate its potential in defining susceptibility profiles based on bacterial morphotyping. The excellent clinical performance of the nanowell AST, combined with a short detection time, morphotyping, and the very low consumption of reagents clearly show the advantage of this phenotypic AST as a diagnostic tool in a clinical setting.

Place, publisher, year, edition, pages
FRONTIERS MEDIA SA, 2018
Keywords
AST, UTI, antibiotic resistance, diagnostics, microfabrication, algorithm
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:kth:diva-232389 (URN)10.3389/fmicb.2018.01530 (DOI)000438072000001 ()2-s2.0-85049866021 (Scopus ID)
Funder
Swedish Research CouncilStockholm County CouncilEU, FP7, Seventh Framework Programme, 615458Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20180726

Available from: 2018-07-26 Created: 2018-07-26 Last updated: 2018-07-26Bibliographically approved
Dias, J. T., Svedberg, G., Nystrand, M., Svahn Andersson, H. & Gantelius, J. (2018). Rapid signal enhancement method for nanoprobe-based biosensing (vol 7, 2017). Scientific Reports, 8(1), Article ID 8184.
Open this publication in new window or tab >>Rapid signal enhancement method for nanoprobe-based biosensing (vol 7, 2017)
Show others...
2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, no 1, article id 8184Article in journal (Refereed) Published
Abstract [en]

In the Methods section of this Article references 18 to 22 are incorrectly cited. The correct references were omitted from the reference list and appear below as references 1-5. References 18 to 22 are correctly cited in Introduction and Results and Discussion sections. "AuNPs of 10 nm in diameter were prepared following the protocol described by Bastus et al.18." should read: "AuNPs of 10 nm in diameter were prepared following the protocol described by Bastus et al.1." "AgNPs of 90 nm in diameter were prepared following the protocol described by Rivero et al.19." should read: "AgNPs of 90 nm in diameter were prepared following the protocol described by Rivero et al.2" "The size was determined by UV-Vis spectroscopy according to the AgNPs size theory demonstrated by Malynych20." should read: "The size was determined by UV-Vis spectroscopy according to the AgNPs size theory demonstrated by Malynych3." "The coupling of antibody to the NPs was prepared following a modified version of a protocol previously reported by Puertas et al.21." should read: "The coupling of antibody to the NPs was prepared following a modified version of a protocol previously reported by Puertas et al.4." "Microarrays were prepared as previously reported by our group22." should read: "Microarrays were prepared as previously reported by our group5.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP, 2018
National Category
Organic Chemistry
Identifiers
urn:nbn:se:kth:diva-230435 (URN)10.1038/s41598-018-26155-4 (DOI)000432657600002 ()29786686 (PubMedID)2-s2.0-85047421736 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20180615

Available from: 2018-06-15 Created: 2018-06-15 Last updated: 2019-10-10Bibliographically approved
Svedberg, G., Jeong, Y., Na, H., Jang, J., Nilsson, P., Kwon, S., . . . Svahn Andersson, H. (2017). Towards encoded particles for highly multiplexed colorimetric point of care autoantibody detection. Lab on a Chip, 17(3), 549-556
Open this publication in new window or tab >>Towards encoded particles for highly multiplexed colorimetric point of care autoantibody detection
Show others...
2017 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 17, no 3, p. 549-556Article in journal (Refereed) Published
Abstract [en]

Highly multiplexed point of care tests could improve diagnostic accuracy and differential diagnostic capacity in for instance emergency medicine and low resource environments. Available technology platforms for POC biomarker detection are typically simplex or low-plexed, whereas common lab-based microarray systems allow for the simultaneous detection of thousands of DNA or protein biomarkers. In this study, we demonstrate a novel suspension particle array platform that utilizes 900 mu m bricks for POC amenable colorimetric biomarker detection with an encoding capacity of over two million. Due to the mm-scale size, both the lithographic codes and colorimetric signals of individual particles can be visualized using a consumer grade office flatbed scanner, with a potential for simultaneous imaging of around 19000 particles per scan. The analytical sensitivity of the assay was determined to be 4 ng ml(-1) using an antibody model system. As a proof of concept, autoantibodies toward anoctamin 2 were detected in order to discriminate between multiple sclerosis plasma samples and healthy controls with p < 0.0001 and an inter-assay % CV of 9.44%.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2017
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-204746 (URN)10.1039/c6lc01358a (DOI)000395887900019 ()28102419 (PubMedID)2-s2.0-85010943844 (Scopus ID)
Funder
VINNOVAEU, FP7, Seventh Framework Programme, 615458
Note

QC 20170601

Available from: 2017-06-01 Created: 2017-06-01 Last updated: 2019-02-20Bibliographically approved
Eissler, N., Mao, Y., Brodin, D., Reuterswärd, P., Svahn Andersson, H., Johnsen, J. I., . . . Kogner, P. (2016). Combination Therapy of Anti-PD-1 Antibody and CSF-1R Inhibitor Reverses Induction of Suppressive Myeloid Cells and Controls Spontaneous Neuroblastoma Progression. Pediatric Blood & Cancer, 63, S28-S28
Open this publication in new window or tab >>Combination Therapy of Anti-PD-1 Antibody and CSF-1R Inhibitor Reverses Induction of Suppressive Myeloid Cells and Controls Spontaneous Neuroblastoma Progression
Show others...
2016 (English)In: Pediatric Blood & Cancer, ISSN 1545-5009, E-ISSN 1545-5017, Vol. 63, p. S28-S28Article in journal, Meeting abstract (Refereed) Published
Place, publisher, year, edition, pages
Wiley-Blackwell, 2016
National Category
Cancer and Oncology Hematology
Identifiers
urn:nbn:se:kth:diva-199552 (URN)000384818800426 ()
Note

QC 20170116

Available from: 2017-01-16 Created: 2017-01-09 Last updated: 2017-11-29Bibliographically approved
Afrasiabi, R., Söderberg, L. M., Jönsson, H., Björk, P., Svahn Andersson, H. & Linnros, J. (2016). Integration of a Droplet-Based Microfluidic System and Silicon Nanoribbon FET Sensor. Micromachines, 7(8)
Open this publication in new window or tab >>Integration of a Droplet-Based Microfluidic System and Silicon Nanoribbon FET Sensor
Show others...
2016 (English)In: Micromachines, ISSN 2072-666X, E-ISSN 2072-666X, Vol. 7, no 8Article in journal (Refereed) Published
Abstract [en]

We present a novel microfluidic system that integrates droplet microfluidics with a silicon nanoribbon field-effect transistor (SiNR FET), and utilize this integrated system to sense differences in pH. The device allows for selective droplet transfer to a continuous water phase, actuated by dielectrophoresis, and subsequent detection of the pH level in the retrieved droplets by SiNR FETs on an electrical sensor chip. The integrated microfluidic system demonstrates a label-free detection method for droplet microfluidics, presenting an alternative to optical fluorescence detection. In this work, we were able to differentiate between droplet trains of one pH-unit difference. The pH-based detection method in our integrated system has the potential to be utilized in the detection of biochemical reactions that induce a pH-shift in the droplets.

Place, publisher, year, edition, pages
MDPI AG, 2016
Keywords
NanoFET; silicon nanoribbon; droplet microfluidics; pH measurement
National Category
Nano Technology
Research subject
Information and Communication Technology
Identifiers
urn:nbn:se:kth:diva-191177 (URN)10.3390/mi7080134 (DOI)000382467700006 ()2-s2.0-84984791952 (Scopus ID)
Funder
Knut and Alice Wallenberg Foundation
Note

QC 20160825. QC 20191126

Available from: 2016-08-25 Created: 2016-08-25 Last updated: 2019-11-26Bibliographically approved
Organisations

Search in DiVA

Show all publications