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Zhou, T., Jie, V. W., Karpov, D., Pacureanu, A., Mokso, R., Romell, J. & Baird, E. (2023). Bumblebee compound eye 3D imaging with x-ray holotomography. In: AIP Conference Proceedings: . Paper presented at 15th International Conference on X-ray Microscopy, XRM 2022, Virtual, Online, Taiwan, Jun 19 2022 - Jun 24 2022. AIP Publishing, Article ID 020012.
Open this publication in new window or tab >>Bumblebee compound eye 3D imaging with x-ray holotomography
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2023 (English)In: AIP Conference Proceedings, AIP Publishing , 2023, article id 020012Conference paper, Published paper (Refereed)
Abstract [en]

Bumblebees are important pollinators, that are declining rapidly in number and need urgent protection. Visual cues are extremely important for bumblebee activities and survival so a full understanding of the micro-and nano-structure of their eyes will help us to better understand their vision and how it is affected by rapid habitat changes. Here, we present the first report on imaging of 3D structures with sub-100 nm resolution of an unstained bumblebee compound eye using X-ray holographic tomography. This study demonstrates the potential of using X-ray nano-tomography for bulk samples for zoological studies on insects. Compared to the other commonly used methods, such as TEM and FIB-SEM, X-ray nanoCT is non-destructive and relatively fast, and has the potential for providing a better understanding of a broad range of biological materials.

Place, publisher, year, edition, pages
AIP Publishing, 2023
Series
AIP Conference Proceedings, ISSN 0094243X
National Category
Zoology
Identifiers
urn:nbn:se:kth:diva-340364 (URN)10.1063/5.0168572 (DOI)2-s2.0-85177603067 (Scopus ID)
Conference
15th International Conference on X-ray Microscopy, XRM 2022, Virtual, Online, Taiwan, Jun 19 2022 - Jun 24 2022
Note

QC 20231206

Available from: 2023-12-06 Created: 2023-12-06 Last updated: 2023-12-06Bibliographically approved
Twengström, W., Moro, C. F., Romell, J., Larsson, J. C., Sparrelid, E., Björnstedt, M. & Hertz, H. (2022). Can laboratory x-ray virtual histology provide intraoperative 3D tumor resection margin assessment?. Journal of Medical Imaging, 9(3), Article ID 031503.
Open this publication in new window or tab >>Can laboratory x-ray virtual histology provide intraoperative 3D tumor resection margin assessment?
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2022 (English)In: Journal of Medical Imaging, ISSN 2329-4302, E-ISSN 2329-4310, Vol. 9, no 3, article id 031503Article in journal (Refereed) Published
Abstract [en]

Purpose:

Surgery is an essential part of the curative plan for most patients affected with solid tumors. The outcome of such surgery, e.g., recurrence rates and ultimately patient survival, depends on several factors where the resection margin is of key importance. Presently, the resection margin is assessed by classical histology, which is time-consuming (several days), destructive, and basically only gives two-dimensional information. Clearly, it would be advantageous if immediate feedback on tumor extension in all three dimensions were available to the surgeon intraoperatively.

Approach:

We investigate a laboratory propagation-based phase-contrast x-ray computed tomography system that provides the resolution, the contrast, and, potentially, the speed for this purpose. The system relies on a liquid-metal jet microfocus source and a scintillator-coated CMOS detector. Our study is performed on paraffin-embedded non-stained samples of human pancreatic neuroendocrine tumors, liver intrahepatic cholangiocarcinoma, and pancreatic serous cystic neoplasm (benign).

Results:

We observe tumors with distinct and sharp edges having cellular resolution (similar to 10 mu m) as well as many assisting histological landmarks, allowing for resection margin assessment. All x-ray data are compared with classical histology. The agreement is excellent.

Conclusion:

We conclude that the method has potential for intraoperative three-dimensional virtual histology.

Place, publisher, year, edition, pages
SPIE-Intl Soc Optical Eng, 2022
Keywords
human resection margin assessment, phase-contrast tomography, propagation-based imaging
National Category
Radiology, Nuclear Medicine and Medical Imaging Cancer and Oncology
Identifiers
urn:nbn:se:kth:diva-315850 (URN)10.1117/1.JMI.9.3.031503 (DOI)000819039300003 ()35155718 (PubMedID)2-s2.0-85133709793 (Scopus ID)
Note

QC 20220721

Available from: 2022-07-21 Created: 2022-07-21 Last updated: 2022-07-21Bibliographically approved
Twengström, W., Moro, C. F., Romell, J., Larsson, J. C., Sparrelid, E., Bjornstedt, M. & Hertz, H. (2021). Can laboratory x-ray virtual histology provide intraoperative 3D tumor resection margin assessment?. In: Muller, B Wang, G (Ed.), DEVELOPMENTS IN X-RAY TOMOGRAPHY XIII: . Paper presented at Conference on Developments in X-Ray Tomography XIII, AUG 01-05, 2021, San Diego, CA. SPIE-Intl Soc Optical Eng, 11840, Article ID 118400Q.
Open this publication in new window or tab >>Can laboratory x-ray virtual histology provide intraoperative 3D tumor resection margin assessment?
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2021 (English)In: DEVELOPMENTS IN X-RAY TOMOGRAPHY XIII / [ed] Muller, B Wang, G, SPIE-Intl Soc Optical Eng , 2021, Vol. 11840, article id 118400QConference paper, Published paper (Refereed)
Abstract [en]

Surgery is an essential part of the curative plan for most patients affected with solid tumors. The outcome of such surgery, e.g., recurrence rates and ultimately patient survival, depends on several factors where the resection margin is of key importance. Presently the resection margin is assessed by classical histology, which is time-consuming (several days), destructive, and basically only gives two-dimensional information. Clearly it would be advantageous if immediate feedback on tumor extension in all three dimensions were available to the surgeon intra-operatively. In the present paper we investigate a laboratory propagation-based phase-contrast x-ray computed tomography (CT) system that provides the resolution, contrast, and, potentially, the speed for this purpose. The system relies on a liquid-metal jet micro-focus source and a scintillator-coated CMOS detector. The study is performed on paraffin-embedded non-stained samples of human pancreatic neuroendocrine tumors, liver intrahepatic cholangiocarcinoma, and pancreatic serous cystic neoplasm (benign). We observe tumors with distinct and sharp edges having cellular resolution (similar to 10 mu m) as well as many assisting histological landmarks, allowing for resection margin assessment. All x-ray data is compared with classical histology. The agreement is excellent, and we conclude that the method has potential for intra-operative three-dimensional virtual histology.

Place, publisher, year, edition, pages
SPIE-Intl Soc Optical Eng, 2021
Series
Proceedings of SPIE, ISSN 0277-786X
National Category
Radiology, Nuclear Medicine and Medical Imaging
Identifiers
urn:nbn:se:kth:diva-315940 (URN)10.1117/12.2593928 (DOI)000825424600022 ()2-s2.0-85123057874 (Scopus ID)
Conference
Conference on Developments in X-Ray Tomography XIII, AUG 01-05, 2021, San Diego, CA
Note

Part of proceedings: ISBN 978-1-5106-4519-6

QC 20220728

Available from: 2022-07-28 Created: 2022-07-28 Last updated: 2022-08-16Bibliographically approved
Romell, J., Jie, V. W., Miettinen, A., Baird, E. & Hertz, H. (2021). Laboratory phase-contrast nanotomography of unstained Bombus terrestris compound eyes. Journal of Microscopy, 283(1), 29-40
Open this publication in new window or tab >>Laboratory phase-contrast nanotomography of unstained Bombus terrestris compound eyes
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2021 (English)In: Journal of Microscopy, ISSN 0022-2720, E-ISSN 1365-2818, Vol. 283, no 1, p. 29-40Article in journal (Refereed) Published
Abstract [en]

Imaging the visual systems of bumblebees and other pollinating insects may increase understanding of their dependence on specific habitats and how they will be affected by climate change. Current high-resolution imaging methods are either limited to two dimensions (light- and electron microscopy) or have limited access (synchrotron radiation x-ray tomography). For x-ray imaging, heavy metal stains are often used to increase contrast. Here, we present micron-resolution imaging of compound eyes of buff-tailed bumblebees (Bombus terrestris) using a table-top x-ray nanotomography (nano-CT) system. By propagation-based phase-contrast imaging, the use of stains was avoided and the microanatomy could more accurately be reconstructed than in samples stained with phosphotungstic acid or osmium tetroxide. The findings in the nano-CT images of the compound eye were confirmed by comparisons with light- and transmission electron microscopy of the same sample and finally, comparisons to synchrotron radiation tomography as well as to a commercial micro-CT system were done.

Place, publisher, year, edition, pages
Wiley, 2021
Keywords
bumblebee, compound eyes, laboratory nano-CT, phase contrast, system comparison, glucose, glutaraldehyde, nanotube, osmium tetraoxide, paraformaldehyde, phosphotungstic acid, acceleration, Article, basement membrane, Bombus terrestris, comparative study, compound eye, controlled study, electric field, electron microscopy, histology, illumination, image processing, micro-computed tomography, nonhuman, phase contrast microscopy, photoreceptor cell, reconstruction algorithm, signal noise ratio, transmission electron microscopy, animal, bee, devices, laboratory, procedures, synchrotron, x-ray computed tomography, Animals, Bees, Laboratories, Microscopy, Phase-Contrast, Osmium Tetroxide, Synchrotrons, Tomography, X-Ray Computed, X-Ray Microtomography
National Category
Radiology, Nuclear Medicine and Medical Imaging
Identifiers
urn:nbn:se:kth:diva-309236 (URN)10.1111/jmi.13005 (DOI)000648344300001 ()33822371 (PubMedID)2-s2.0-85105346424 (Scopus ID)
Note

QC 20220301

Available from: 2022-03-01 Created: 2022-03-01 Last updated: 2022-06-25Bibliographically approved
Romell, J. (2021). Virtual histology by laboratory x-ray phase-contrast tomography. (Doctoral dissertation). KTH Royal Institute of Technology
Open this publication in new window or tab >>Virtual histology by laboratory x-ray phase-contrast tomography
2021 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Detailed imaging of biological samples is central to different fields of research, as well as for clinical pathology. Classical histology, using light- orelectron microscopy, can generate high-resolution images but is destructive and only gives two-dimensional information. With virtual histology, athree-dimensional reconstruction of the sample is instead generated, whichcan be virtually sectioned in arbitrary directions. This Thesis presents developments of x-ray phase-contrast computed tomography (CT) as a tool forvirtual histological analysis. In particular, the focus is imaging with laboratory systems as opposed to at large-scale synchrotron facilities, and usingphase-contrast imaging as an alternative to chemical contrast agents.A broad range of imaging applications are demonstrated, from archaeology to clinical pathology, where acquisition and data processing have beenoptimised for each sample. A micro-CT system based on a liquid-metaljet x-ray source was used for imaging centimetre-sized samples. Threedimensional imaging of mummified remains was demonstrated, with sufficient contrast and resolution within the soft tissue to capture microanatomical details; blood vessels, skin layers, fingerprints and remains of adiposecells were visualised in the hand of an ancient Egyptian mummy. Virtualhistology was also performed on paraffin-embedded excised tumours foranalysis of the resection margin, and on rat bones for mapping the vascularcanals.For imaging with micrometre resolution, a nano-CT system was builtand characterised. Photoreceptor imaging in unstained compound eyes ofbumblebees was demonstrated, and light- and electron microscopy wereused to verify the results. Comparisons with conventional attenuationmicro-CT and synchrotron radiation tomography, with both unstained andstained samples, showed the advantage of using phase contrast instead ofstaining the samples

Abstract [sv]

Detaljerad avbildning av biologiska prover är viktigt för olika forskningsområden såväl som för klinisk patologi. Klassisk histologi, genom ljus- ellerelektronmikroskopi, ger högupplösta bilder men är destruktiv och kan endast ge tvådimensionell information. Med virtuell histologi genereras istället en tredimensionell rekonstruktion av provet, vilken kan analyseras genom snittning i godtyckliga riktningar. Den här Avhandlingen presenterarvidareutvecklingar av faskontrast-baserad röntgentomografi (CT), som ettverktyg för virtuell histologi. I synnerhet ligger fokus på avbildning medlabbaserade system, i motsats till storskaliga synkrotronljusanläggningar,och på faskontrastavbildning som ett alternativ till kemiska kontrastmedel.En bredd av olika tillämpningar presenteras, från arkeologi till kliniskpatologi, med datainsamling och databehandling anpassade för varje prov.Ett mikro-CT-system baserat på en metallstråle-röntgenkälla användes föravbildning av centimeterstora prover. Tredimensionell avbildning av mumifierade kvarlevor demonstrerades, med tillräcklig kontrast och upplösning i mjukvävnader för att fånga mikroanatomiska detaljer; blodkärl, hudlager, fingeravtryck och rester av fettceller kunde visualiseras i en egyptiskmumiehand. Virtuell histologi tillämpades också på paraffininbäddade tumörer för analys av resektionsmarginal, och på råttben för avbildning avvaskulära kanaler.För avbildning med mikrometerupplösning byggdes ett nano-CTsystem. Efter karaktärisering av systemet demonstrerades avbildning avfotoreceptorer i ofärgade facettögon från humlor. Ljus- och elektronmikroskopi användes för att verifiera resultaten. Jämförelser med konventionellattenuationsbaserad mikro-CT samt synkrotronljustomografi, både medofärgade och färgade prover, visade på fördelarna med användning avfaskontrast istället för att färga proverna.

Place, publisher, year, edition, pages
KTH Royal Institute of Technology, 2021. p. 69
Series
TRITA-SCI-FOU ; 2021:07
National Category
Radiology, Nuclear Medicine and Medical Imaging
Research subject
Physics, Biological and Biomedical Physics
Identifiers
urn:nbn:se:kth:diva-290960 (URN)978-91-7873-806-9 (ISBN)
Public defence
2021-03-26, Via zoom https://kth-se.zoom.us/j/66938252095, 13:00 (English)
Opponent
Supervisors
Available from: 2021-03-01 Created: 2021-02-26 Last updated: 2022-06-25Bibliographically approved
Romell, J., Häggmark, I., Twengström, W., Romell, M., Häggman, S., Ikram, S. & Hertz, H. (2019). Virtual histology of dried and mummified biological samples by laboratory phase-contrast tomography. In: X-Ray Nanoimaging: Instruments and Methods IV. Paper presented at X-Ray Nanoimaging: Instruments and Methods IV 2019; San Diego; United States; 11 August 2019 through 12 August 2019. SPIE - The International Society for Optics and Photonics, 11112, Article ID 111120S.
Open this publication in new window or tab >>Virtual histology of dried and mummified biological samples by laboratory phase-contrast tomography
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2019 (English)In: X-Ray Nanoimaging: Instruments and Methods IV, SPIE - The International Society for Optics and Photonics, 2019, Vol. 11112, article id 111120SConference paper, Published paper (Refereed)
Abstract [en]

Ancient remains from humans, animals and plants hold valuable information about our history. X-ray imaging methods are often, because of their non-destructive nature, used in the analysis of such samples. The classical x-ray imaging methods, radiography and computed tomography (CT), are based on absorption, which works well for radiodense structures like bone, but gives limited contrast for textiles and soft tissues, which exhibit high x-ray transmission. Destructive methods, such as classical histology, have historically been used for analysing ancient soft tissue but the extent to which it is used today is limited because of the fragility and value of many ancient samples. For detailed, non-destructive analysis of ancient biological samples, we instead propose x-ray phase-contrast CT, which like conventional CT gives volume data but with the possibility of better resolution through the detection of phase shift. Using laboratory x-ray sources, we here demonstrate the capabilities of phase-contrast tomography of dried biological samples. Virtual histological analysis of a mummified human hand from ancient Egypt is performed, revealing remains of adipose cells in situ, which would not be possible with classical histology. For higher resolution, a lab-based nano-CT arrangement based on a nanofocus transmission x-ray source is presented. With an x-ray emission spot of 300 nm the system shows potential for sub-micronresolution 3D imaging. For characterisation of the performance of phase-contrast imaging of dried samples a piece of wood is imaged. Finally, we present the first phase-contrast CT data from our nano-CT system, acquired of the dried head of a bee.

Place, publisher, year, edition, pages
SPIE - The International Society for Optics and Photonics, 2019
Series
Proceedings of SPIE - The International Society for Optical Engineering, ISSN 0277-786X ; 11112
Keywords
Archaeology, Liquid-metal-jet x-ray source, Mummy, Nanofocus x-ray source, Phase-contrast tomography, Virtual histology
National Category
Medical Engineering
Identifiers
urn:nbn:se:kth:diva-266659 (URN)10.1117/12.2531163 (DOI)000511110600016 ()2-s2.0-85076557974 (Scopus ID)
Conference
X-Ray Nanoimaging: Instruments and Methods IV 2019; San Diego; United States; 11 August 2019 through 12 August 2019
Note

Part of ISBN 9781510629172

QC 20200116

Available from: 2020-01-16 Created: 2020-01-16 Last updated: 2024-03-15Bibliographically approved
Zdora, M.-C., Zanette, I., Zhou, T., Koch, F. J., Romell, J., Sala, S., . . . Thibault, P. (2018). At-wavelength optics characterisation via X-ray speckle- and grating-based unified modulated pattern analysis. Optics Express, 26(4), 4989-5004
Open this publication in new window or tab >>At-wavelength optics characterisation via X-ray speckle- and grating-based unified modulated pattern analysis
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2018 (English)In: Optics Express, E-ISSN 1094-4087, Vol. 26, no 4, p. 4989-5004Article in journal (Refereed) Published
Abstract [en]

The current advances in new generation X-ray sources are calling for the development and improvement of high-performance optics. Techniques for high-sensitivity phase sensing and wavefront characterisation, preferably performed at-wavelength, are increasingly required for quality control, optimisation and development of such devices. We here show that the recently proposed unified modulated pattern analysis (UMPA) can be used for these purposes. We characterised two polymer X-ray refractive lenses and quantified the effect of beam damage and shape errors on their refractive properties. Measurements were performed with two different setups for UMPA and validated with conventional X-ray grating interferometry. Due to its adaptability to different setups, the ease of implementation and cost-effectiveness, we expect UMPA to find applications for high-throughput quantitative optics characterisation and wavefront sensing.

Place, publisher, year, edition, pages
Optical Society of America, 2018
National Category
Other Physics Topics
Identifiers
urn:nbn:se:kth:diva-224701 (URN)10.1364/OE.26.004989 (DOI)000426268500112 ()29475342 (PubMedID)2-s2.0-85042115138 (Scopus ID)
Funder
EU, FP7, Seventh Framework Programme, StG 279753
Note

QC 20180323

Available from: 2018-03-23 Created: 2018-03-23 Last updated: 2024-03-18Bibliographically approved
Häggmark, I., Romell, J., Lewin, S., Öhman, C. & Hertz, H. (2018). Cellular-Resolution Imaging of Microstructures in Rat Bone using Laboratory Propagation-Based Phase-Contrast X-ray Tomography. In: Microscopy and Microanalysis: . Paper presented at 14th International Conference on X-ray Microscopy (pp. 368-369). , 24
Open this publication in new window or tab >>Cellular-Resolution Imaging of Microstructures in Rat Bone using Laboratory Propagation-Based Phase-Contrast X-ray Tomography
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2018 (English)In: Microscopy and Microanalysis, 2018, Vol. 24, p. 368-369Conference paper, Published paper (Refereed)
National Category
Medical Image Processing Radiology, Nuclear Medicine and Medical Imaging
Identifiers
urn:nbn:se:kth:diva-240273 (URN)10.1017/S1431927618014137 (DOI)
Conference
14th International Conference on X-ray Microscopy
Note

QC 20181217

Available from: 2018-12-14 Created: 2018-12-14 Last updated: 2022-06-26Bibliographically approved
Romell, J. (2018). Imaging fingerprints of an ancient Egyptian mummy by propagation-based phase-contrast x-ray tomography. In: Proceedings of the 14th International Conference on X-ray Microscopy (XRM2018): . Paper presented at 14th International Conference on X-ray Microscopy, Saskatoon, Saskatchewan, Canada, from August 19-24, 2018..
Open this publication in new window or tab >>Imaging fingerprints of an ancient Egyptian mummy by propagation-based phase-contrast x-ray tomography
2018 (English)In: Proceedings of the 14th International Conference on X-ray Microscopy (XRM2018), 2018Conference paper, Published paper (Refereed)
National Category
Medical Image Processing Atom and Molecular Physics and Optics
Identifiers
urn:nbn:se:kth:diva-290957 (URN)
Conference
14th International Conference on X-ray Microscopy, Saskatoon, Saskatchewan, Canada, from August 19-24, 2018.
Note

QC 20210301

Available from: 2021-02-26 Created: 2021-02-26 Last updated: 2022-06-25Bibliographically approved
Romell, J., Vågberg, W., Romell, M., Haggman, S., Ikram, S. & Hertz, H. (2018). Soft-Tissue Imaging in a Human Mummy: Propagation-based Phase-Contrast CT. Radiology, 289(3), 670-676
Open this publication in new window or tab >>Soft-Tissue Imaging in a Human Mummy: Propagation-based Phase-Contrast CT
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2018 (English)In: Radiology, ISSN 0033-8419, E-ISSN 1527-1315, Vol. 289, no 3, p. 670-676Article in journal (Refereed) Published
Abstract [en]

Purpose: To evaluate phase-contrast CT as a noninvasive alternative to histology in the study of ancient soft tissue. Materials and Methods: The imaging was performed between May 8 and June 13, 2017. A mummified human hand from ancient Egypt was imaged in a laboratory phase-contrast CT arrangement with propagation-based imaging. The experimental arrangement for propagation-based imaging included a microfocus x-ray source, a rotation stage for the sample, and an x-ray detector. The mummified hand was imaged in two different modes. First, a CT scan of the whole hand was performed in an overview arrangement. Then, a detailed scan of the tip of the middle finger was performed. With imaging distances tailored fora large magnification and to maximize die phase-contrast signal, the estimated resolution in the final images was 6-9 mu m. Results: The overview CT allowed identification tendons of the hand, as well as identification of arteries and nerves in the dehydrated soft tissue. In the detailed phase-contrast setting, virtual histology of the soft tissues of the fingertip could be performed. Blood vessels in the nail bed and the microanatomy of the bone marrow and hypodermis were imaged, and the layers of the skin could be distinguished. Round structures in the adipose tissue were identified as the reamins of adipocytes. Conclusion: Laboratory phase-contrast CT enables imaging of the anatomy and microanatomy of mummified soft tissue with sub-10-mu m resolution and may serve as a complement or alternative to the classic invasive histrologic methods used in soft-tissue paleopathology. (C) RSNA.2018

Place, publisher, year, edition, pages
RADIOLOGICAL SOC NORTH AMERICA, 2018
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-239987 (URN)10.1148/radiol.2018180945 (DOI)000450569200015 ()30251933 (PubMedID)2-s2.0-85056714548 (Scopus ID)
Funder
Swedish Research CouncilWallenberg Foundations
Note

QC 20181211

Available from: 2018-12-11 Created: 2018-12-11 Last updated: 2022-12-06Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-6716-1795

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