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Wiklund, Martin
Publications (10 of 12) Show all publications
Saeidi, D., Saghafian, M., Javanmard, S. H., Hammarström, B. & Wiklund, M. (2019). Acoustic dipole and monopole effects in solid particle interaction dynamics during acoustophoresis. Journal of the Acoustical Society of America, 145(6), 3311-3319
Open this publication in new window or tab >>Acoustic dipole and monopole effects in solid particle interaction dynamics during acoustophoresis
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2019 (English)In: Journal of the Acoustical Society of America, ISSN 0001-4966, E-ISSN 1520-8524, Vol. 145, no 6, p. 3311-3319Article in journal (Refereed) Published
Abstract [en]

A method is presented for measurements of secondary acoustic radiation forces acting on solid particles in a plain ultrasonic standing wave. The method allows for measurements of acoustic interaction forces between particles located in arbitrary positions such as in between a pressure node and a pressure antinode. By utilizing a model that considers both density- and compressibility-dependent effects, the observed particle-particle interaction dynamics can be well understood. Two differently sized polystyrene micro-particles (4.8 and 25 mu m, respectively) were used in order to achieve pronounced interaction effects. The particulate was subjected to a 2-MHz ultrasonic standing wave in a microfluidic channel, such as commonly used for acoustophoresis. Observation of deflections in the particle pathways shows that the particle interaction force is not negligible under this circumstance and has to be considered in accurate particle manipulation applications. The effect is primarily pronounced when the distance between two particles is small, the sizes of the particles are different, and the acoustic properties of the particles are different relative to the media. As predicted by theory, the authors also observe that the interaction forces are affected by the angle between the inter-particle centerline and the axis of the standing wave propagation direction.

Place, publisher, year, edition, pages
ACOUSTICAL SOC AMER AMER INST PHYSICS, 2019
National Category
Meteorology and Atmospheric Sciences
Identifiers
urn:nbn:se:kth:diva-255491 (URN)10.1121/1.5110303 (DOI)000474603700029 ()31255151 (PubMedID)2-s2.0-85067078144 (Scopus ID)
Note

QC 20190919

Available from: 2019-09-19 Created: 2019-09-19 Last updated: 2019-09-19Bibliographically approved
Delsing, P., Wiklund, M. & Westerhausen, C. (2019). The 2019 surface acoustic waves roadmap. Journal of Physics D: Applied Physics, 52(35), Article ID 353001.
Open this publication in new window or tab >>The 2019 surface acoustic waves roadmap
2019 (English)In: Journal of Physics D: Applied Physics, ISSN 0022-3727, E-ISSN 1361-6463, Vol. 52, no 35, article id 353001Article, review/survey (Refereed) Published
Abstract [en]

Today, surface acoustic waves (SAWs) and bulk acoustic waves are already two of the very few phononic technologies of industrial relevance and can been found in a myriad of devices employing these nanoscale earthquakes on a chip. Acoustic radio frequency filters, for instance, are integral parts of wireless devices. SAWs in particular find applications in life sciences and microfluidics for sensing and mixing of tiny amounts of liquids. In addition to this continuously growing number of applications, SAWs are ideally suited to probe and control elementary excitations in condensed matter at the limit of single quantum excitations. Even collective excitations, classical or quantum are nowadays coherently interfaced by SAWs. This wide, highly diverse, interdisciplinary and continuously expanding spectrum literally unites advanced sensing and manipulation applications. Remarkably, SAW technology is inherently multiscale and spans from single atomic or nanoscopic units up even to the millimeter scale. The aim of this Roadmap is to present a snapshot of the present state of surface acoustic wave science and technology in 2019 and provide an opinion on the challenges and opportunities that the future holds from a group of renown experts, covering the interdisciplinary key areas, ranging from fundamental quantum effects to practical applications of acoustic devices in life science.

Place, publisher, year, edition, pages
IOP PUBLISHING LTD, 2019
Keywords
surface acoustic waves, phononics, quantum acoustics
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-255401 (URN)10.1088/1361-6463/ab1b04 (DOI)000473816400001 ()
Note

QC 20190814

Available from: 2019-08-14 Created: 2019-08-14 Last updated: 2019-08-14Bibliographically approved
Olofsson, K., Carannante, V., Ohlin, M., Frisk, T., Kushiro, K., Takai, M., . . . Wiklund, M. (2018). Acoustic formation of multicellular tumor spheroids enabling on-chip functional and structural imaging. Lab on a Chip, 18(16), 2466-2476
Open this publication in new window or tab >>Acoustic formation of multicellular tumor spheroids enabling on-chip functional and structural imaging
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2018 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 18, no 16, p. 2466-2476Article in journal (Refereed) Published
Abstract [en]

Understanding the complex 3D tumor microenvironment is important in cancer research. This microenvironment can be modelled in vitro by culturing multicellular tumor spheroids (MCTS). Key challenges when using MCTS in applications such as high-throughput drug screening are overcoming imaging and analytical issues encountered during functional and structural investigations. To address these challenges, we use an ultrasonic standing wave (USW) based MCTS culture platform for parallel formation, staining and imaging of 100 whole MCTS. A protein repellent amphiphilic polymer coating enables flexible production of high quality and unanchored MCTS. This enables high-content multimode analysis based on flow cytometry and in situ optical microscopy. We use HepG2 hepatocellular carcinoma, A498 and ACHN renal carcinoma, and LUTC-2 thyroid carcinoma cell lines to demonstrate (i) the importance of the ultrasound-coating combination, (ii) bright field image based automatic characterization of MTCS, (iii) detailed deep tissue confocal imaging of whole MCTS mounted in a refractive index matching solution, and (iv) single cell functional analysis through flow cytometry of single cell suspensions of disintegrated MTCS. The USW MCTS culture platform is customizable and holds great potential for detailed multimode MCTS analysis in a high-content manner.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2018
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
urn:nbn:se:kth:diva-234606 (URN)10.1039/c8lc00537k (DOI)000442265100012 ()30033460 (PubMedID)2-s2.0-85051357097 (Scopus ID)
Funder
Stockholm County CouncilSwedish Cancer SocietySwedish Childhood Cancer FoundationSwedish Research CouncilSwedish Foundation for Strategic Research
Note

QC 20180914

Available from: 2018-09-14 Created: 2018-09-14 Last updated: 2018-09-14Bibliographically approved
Faridi, M. A., Shahzad, A. F., Russom, A. & Wiklund, M. (2018). Milliliter scale acoustophoresis based bioparticle processing platform. In: ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018: . Paper presented at ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018, Dubrovnik, Croatia, 10 June 2018 through 13 June 2018. ASME Press
Open this publication in new window or tab >>Milliliter scale acoustophoresis based bioparticle processing platform
2018 (English)In: ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018, ASME Press, 2018Conference paper, Published paper (Refereed)
Abstract [en]

Bioparticles such as mammalian cells and bacteria can be manipulated directly or indirectly for multiple applications such as sample preparation for diagnostic applications mainly up-concentration, enrichment & separation as well as immunoassay development. There are various active and passive microfluidic particle manipulation techniques where Acoustophoresis is a powerful technique showing high cell viability. The use of disposable glass capillaries for acoustophoresis, instead of cleanroom fabricated glass-silicon chip can potentially bring down the cost factor substantially, aiding the realization of this technique for real-world diagnostic devices. Unlike available chips and capillary-based microfluidic devices, we report milliliter-scale platform able to accommodate 1ml of a sample for acoustophoresis based processing on a market available glass capillary. Although it is presented as a generic platform but as a demonstration we have shown that polystyrene suspending medium sample can be processed with trapping efficiency of 87% and the up-concentration factor of 10 times in a flow through manner i.e., at 35µl/min. For stationary volume accommodation, this platform practically offers 50 times more sample handling capacity than most of the microfluidic setups. Furthermore, we have also shown that with diluted blood (0.6%) in a flow-through manner, 82% of the white blood cells (WBCs) per ml could be kept trapped. This milliliter platform could potentially be utilized for assisting in sample preparation, plasma separation as well as a flow-through immunoassay assay development for clinical diagnostic applications.

Place, publisher, year, edition, pages
ASME Press, 2018
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:kth:diva-238419 (URN)2-s2.0-85053923284 (Scopus ID)9780791851197 (ISBN)
Conference
ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018, Dubrovnik, Croatia, 10 June 2018 through 13 June 2018
Note

QC 20181108

Available from: 2018-11-08 Created: 2018-11-08 Last updated: 2018-11-08Bibliographically approved
Olofsson, K., Hammarström, B. & Wiklund, M. (2018). Ultrasonic Based Tissue Modelling and Engineering. Micromachines, 9(11), Article ID 594.
Open this publication in new window or tab >>Ultrasonic Based Tissue Modelling and Engineering
2018 (English)In: Micromachines, ISSN 2072-666X, E-ISSN 2072-666X, Vol. 9, no 11, article id 594Article, review/survey (Refereed) Published
Abstract [en]

Systems and devices for in vitro tissue modelling and engineering are valuable tools, which combine the strength between the controlled laboratory environment and the complex tissue organization and environment in vivo. Device-based tissue engineering is also a possible avenue for future explant culture in regenerative medicine. The most fundamental requirements on platforms intended for tissue modelling and engineering are their ability to shape and maintain cell aggregates over long-term culture. An emerging technology for tissue shaping and culture is ultrasonic standing wave (USW) particle manipulation, which offers label-free and gentle positioning and aggregation of cells. The pressure nodes defined by the USW, where cells are trapped in most cases, are stable over time and can be both static and dynamic depending on actuation schemes. In this review article, we highlight the potential of USW cell manipulation as a tool for tissue modelling and engineering.

Place, publisher, year, edition, pages
MDPI, 2018
Keywords
acoustic trapping, ultrasonic manipulation, tissue engineering, tissue modelling, acoustofluidics, microfluidics
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-240005 (URN)10.3390/mi9110594 (DOI)000451314900057 ()30441752 (PubMedID)2-s2.0-85057017318 (Scopus ID)
Note

QC 20181210

Available from: 2018-12-10 Created: 2018-12-10 Last updated: 2018-12-10Bibliographically approved
Faridi, M. A., Ramachandraiah, H., Iranmanesh, I. S., Grishenkov, D., Wiklund, M. & Russom, A. (2017). MicroBubble Activated Acoustic Cell Sorting: BAACS. Biomedical microdevices (Print), 19(2), Article ID 23.
Open this publication in new window or tab >>MicroBubble Activated Acoustic Cell Sorting: BAACS
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2017 (English)In: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 19, no 2, article id 23Article in journal (Refereed) Published
Abstract [en]

Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting applications. However, one of the major impediments for routine use of acoustophoresis at clinical laboratory has been the reliance on the inherent physical properties of cells for separation. Here, we present a microfluidic-based microBubble-Activated Acoustic Cell Sorting (BAACS) method that rely on the specific binding of target cells to microbubbles conjugated with specific antibodies on their surface for continuous cell separation using ultrasonic standing wave. In acoustophoresis, cells being positive acoustic contrast particles migrate to pressure nodes. On the contrary we show that air-filled polymer-shelled microbubbles being strong negative acoustic contrast particles migrate to pressure antinodes at acoustic pressure amplitudes as low as 60 kPa. As a proof of principle, using the BAACS strategy, we demonstrate the separation of cancer cell line in a suspension with better than 75% efficiency. Moreover, 100% of the microbubble-cell conjugates migrated to the anti-node. Hence a better upstream affinity-capture has the potential to provide higher sorting efficiency. The BAACS technique may potentially provide a simplistic approach for similar sized selective isolation of cells, and is suited for applications in point of care.

Place, publisher, year, edition, pages
Springer, 2017
Keywords
Cell sorting, acoustophoresis, microbubble, contrast agent, microfluidic separation
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-205293 (URN)10.1007/s10544-017-0157-4 (DOI)000400547000005 ()28374278 (PubMedID)2-s2.0-85016958658 (Scopus ID)
Funder
EU, FP7, Seventh Framework Programme, 115153Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20170515

Available from: 2017-04-12 Created: 2017-04-12 Last updated: 2019-01-30Bibliographically approved
Carannante, V., Olofsson, K., Van Oojen, H., Edwards, S., Brismar, H., Lundqvist, A., . . . Önfelt, B. (2017). Novel platform for studying infiltration, migration and cytotoxicity of human Natural Killer cells in solid tumors. Paper presented at 44th Annual Meeting of the Scandinavian-Society-for-Immunology (SSI), OCT 17-20, 2017, Stockholm, SWEDEN. Scandinavian Journal of Immunology, 86(4), 315-315
Open this publication in new window or tab >>Novel platform for studying infiltration, migration and cytotoxicity of human Natural Killer cells in solid tumors
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2017 (English)In: Scandinavian Journal of Immunology, ISSN 0300-9475, E-ISSN 1365-3083, Vol. 86, no 4, p. 315-315Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
WILEY, 2017
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-215794 (URN)000411865200163 ()
Conference
44th Annual Meeting of the Scandinavian-Society-for-Immunology (SSI), OCT 17-20, 2017, Stockholm, SWEDEN
Note

QC 20171018

Available from: 2017-10-18 Created: 2017-10-18 Last updated: 2017-10-18Bibliographically approved
Faridi, M. A., Ramachandraiah, H., Iranmanesh, I. S., Grishenkov, D., Wiklund, M. & Russom, A. (2016). Microbubble assisted cell sorting by acoustophoresis. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016: . Paper presented at 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016 (pp. 1677-1678). Chemical and Biological Microsystems Society
Open this publication in new window or tab >>Microbubble assisted cell sorting by acoustophoresis
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2016 (English)In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society , 2016, p. 1677-1678Conference paper, Published paper (Refereed)
Abstract [en]

Polymer shelled gas microbubbles (MBs) are used to sort cells in a microfluidic chip under acoustic standing waves (SW). When particles are subjected to SW based on their acoustic contrast factor (ACF) they migrate to nodes (positive contrast factor particles; PACP) or antinodes (negative acoustic contrast particles; NACP)[1]. We have bounded functionalized MBs with cells such that, they can be selectively migrated to antinodes under SW and sorted from unbounded cell both in no flow and flow conditions. Here we demonstrate acoustic mediated microbubble tagged cell sorting with 75% efficiency.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society, 2016
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-207568 (URN)2-s2.0-85014178442 (Scopus ID)9780979806490 (ISBN)
Conference
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016
Note

Conference code: 126047; Export Date: 22 May 2017; Conference Paper; Correspondence Address: Faridi, M.A.; School of Biotechnology, Royal Institute of Technology KTHSweden; email: mafaridi@kth.se. QC 20170530

Available from: 2017-05-30 Created: 2017-05-30 Last updated: 2017-05-30Bibliographically approved
Hsu, H.-T., Mace, E. M., Carisey, A. F., Viswanath, D. I., Christakou, A., Wiklund, M., . . . Orange, J. S. (2016). NK cells converge lytic granules to promote cytotoxicity and prevent bystander killing. Journal of Cell Biology, 215(6), 875-889
Open this publication in new window or tab >>NK cells converge lytic granules to promote cytotoxicity and prevent bystander killing
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2016 (English)In: Journal of Cell Biology, ISSN 0021-9525, E-ISSN 1540-8140, Vol. 215, no 6, p. 875-889Article in journal (Refereed) Published
Abstract [en]

Natural killer (NK) cell activation triggers sequential cellular events leading to destruction of diseased cells. We previously identified lytic granule convergence, a dynein-and integrin signal-dependent movement of lysosome-related organelles to the microtubule-organizing center, as an early step in the cell biological process underlying NK cell cytotoxicity. Why lytic granules converge during NK cell cytotoxicity, however, remains unclear. We experimentally controlled the availability of human ligands to regulate NK cell signaling and promote granule convergence with either directed or nondirected degranulation. By the use of acoustic trap microscopy, we generated specific effector-target cell arrangements to define the impact of the two modes of degranulation. NK cells with converged granules had greater targeted and less nonspecific "bystander" killing. Additionally, NK cells in which dynein was inhibited or integrin blocked under physiological conditions demonstrated increased nondirected degranulation and bystander killing. Thus, NK cells converge lytic granules and thereby improve the efficiency of targeted killing and prevent collateral damage to neighboring healthy cells.

Place, publisher, year, edition, pages
Rockefeller University Press, 2016
National Category
Cell Biology
Identifiers
urn:nbn:se:kth:diva-199737 (URN)10.1083/jcb.201604136 (DOI)000390414900014 ()2-s2.0-85009183766 (Scopus ID)
Note

QC 20170123

Available from: 2017-01-23 Created: 2017-01-16 Last updated: 2017-11-29Bibliographically approved
Olofsson, K., Carannante, V., Frisk, T., Kushiro, K., Takai, M., Önfelt, B. & Wiklund, M. (2016). Unanchored micro-tumors in an ultrasonic actuated multi-well microplate with protein repellent coating. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016: . Paper presented at 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016 (pp. 409-410). Chemical and Biological Microsystems Society
Open this publication in new window or tab >>Unanchored micro-tumors in an ultrasonic actuated multi-well microplate with protein repellent coating
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2016 (English)In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society , 2016, p. 409-410Conference paper, Published paper (Refereed)
Abstract [en]

In this paper we demonstrate an improved tissue engineering method producing 100 three-dimensional (3D) HepG2 cell structures in parallel based on a combination of ultrasonic actuation and polymer coating in a multi-well microplate. By the use of a polymer coating in the plates, the method creates non-adherent tumor models of controlled size and shape which introduces both a more flexible 3D culture system as well as improved quality of the 3D tumor relative to previous studies [1].

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society, 2016
Keywords
3D cell culture, Polymer coating, Ultrasonic trapping, Cell culture, Cell engineering, Coatings, Polymers, Tissue engineering, Tumors, 3-D cell culture, 3-D culture system, Controlled size, Hep-g2 cells, Threedimensional (3-d), Tumor models, Plastic coatings
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-207518 (URN)2-s2.0-85014291559 (Scopus ID)9780979806490 (ISBN)
Conference
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016
Note

Conference code: 126047; Export Date: 22 May 2017; Conference Paper. QC 20170607

Available from: 2017-06-07 Created: 2017-06-07 Last updated: 2017-06-07Bibliographically approved
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