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Russom, Aman, Assoc.Prof.ORCID iD iconorcid.org/0000-0002-0242-358X
Publications (10 of 15) Show all publications
Bose, I., Ohlander, A., Kutter, C. & Russom, A. (2018). An integrated all foil based micro device for point of care diagnostic applications. Sensors and actuators. B, Chemical, 259, 917-925
Open this publication in new window or tab >>An integrated all foil based micro device for point of care diagnostic applications
2018 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 259, p. 917-925Article in journal (Refereed) Published
Abstract [en]

Point-of-Care (POC) diagnostics often fail to meet the market requirements of low cost and advanced functionality, and are often limited to lateral flow based serological diagnostics with reduced sensitivity and specificity. We report here on an integrated microfluidic absorbance measurement device fabricated by roll-to-roll (R2R) compatible manufacturing processes, suitable for low cost POC systems. It is a device exclusively made of foils and takes external light from a low cost LED and converts the point light source to a homogeneous light via a foil based optical filter at the bottom of the device. The light is converted to an electrical signal by an amorphous organic semiconductor (OSC) material, integrated with screen-printed carbon finger on top of the device for electrical measurement. As a proof of principle, we demonstrate DNA hybridization assay, where the target DNA is coupled to magnetic beads for absorbance measurement. The device successfully distinguishes between matched and mismatched DNA hybridization and can differentiate between 1 μM, 50 nM and 2.5 nM DNA target concentrations. The inherent characteristics of the substrates and R2R fabrication concept significantly reduce the cost, making it suitable for POC applications at resource-limited settings. 

Place, publisher, year, edition, pages
Elsevier B.V., 2018
Keyword
DNA analysis, Microfluidics, Optical detection, Point-of-care, Amorphous carbon, Carbon, Costs, DNA, Light sources, Organic light emitting diodes (OLED), Substrates, Absorbance measurements, Dna hybridization assays, Inherent characteristics, Integrated microfluidics, Point of care, Point of care diagnostic, Cost reduction
National Category
Biological Sciences Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-223112 (URN)10.1016/j.snb.2017.12.133 (DOI)000424877600111 ()2-s2.0-85039735667 (Scopus ID)
Note

Export Date: 13 February 2018; Article; CODEN: SABCE; Correspondence Address: Russom, A.; Div of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH Royal Institute of TechnologySweden; email: aman.russom@scilifelab.se. QC 20180327

Available from: 2018-03-27 Created: 2018-03-27 Last updated: 2018-03-27Bibliographically approved
Zhang, W., Ambikan, A. T., Sperk, M., van Domselaar, R., Nowak, P., Noyan, K., . . . Neogi, U. (2018). Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers. EBioMedicine, 27, 40-50
Open this publication in new window or tab >>Transcriptomics and Targeted Proteomics Analysis to Gain Insights Into the Immune-control Mechanisms of HIV-1 Infected Elite Controllers
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2018 (English)In: EBioMedicine, ISSN 0360-0637, E-ISSN 2352-3964, Vol. 27, p. 40-50Article in journal (Refereed) Published
Abstract [en]

A small subset of HIV-1 infected individuals, the "Elite Controllers" (EC), can control viral replication and restrain progression to immunodeficiency without antiretroviral therapy (ART). In this study, a cross-sectional transcriptomics and targeted proteomics analysis were performed in a well-defined Swedish cohort of untreated EC (n = 19), treatment naive patients with viremia (VP, n = 32) and HIV-1-negative healthy controls (HC, n = 23). The blood transcriptome identified 151 protein-coding genes that were differentially expressed (DE) in VP compared to EC. Genes like CXCR6 and SIGLEC1were downregulated in EC compared to VP. A definite distinction in gene expression between males and females among all patient-groups were observed. The gene expression profile between female EC and the healthy females was similar but did differ between male EC and healthy males. At targeted proteomics analysis, 90% (29/32) of VPs clustered together while EC and HC clustered separately from VP. Among the soluble factors, 33 were distinctive to be statistically significant (False discovery rate = 0.02). Cell surface receptor signaling pathway, programmed cell death, response to cytokine and cytokine-mediated signaling seem to synergistically play an essential role in HIV-1 control in EC.

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV, 2018
Keyword
HIV-1 Elite Controllers, Transcriptome, Proteome
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:kth:diva-224058 (URN)10.1016/j.ebiom.2017.11.031 (DOI)000425875400009 ()29269040 (PubMedID)
Note

QC 20180316

Available from: 2018-03-16 Created: 2018-03-16 Last updated: 2018-03-16Bibliographically approved
Aljadi, Z., Nopp, A., Winqvist, O., Russom, A., Hylander, B., Jacobson, S. H. & Lundahl, J. (2017). Altered basophil function in patients with chronic kidney disease on hemodialysis. Clinical Nephrology, 88(2), 86-96
Open this publication in new window or tab >>Altered basophil function in patients with chronic kidney disease on hemodialysis
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2017 (English)In: Clinical Nephrology, ISSN 0301-0430, Vol. 88, no 2, p. 86-96Article in journal (Refereed) Published
Abstract [en]

Aims: Chronic kidney disease (CKD) leads to impairment of immune cell function. Given the potential role of basophils in the pathogenesis of CKD, we aimed to study the basophil responsiveness towards microbial antigen exposure, judged as adhesion molecule expression and degranulation, in CKD patients on hemodialysis. Materials and methods: We selected markers linked to two crucial biological phases: the transmigration and degranulation processes, respectively. For the transmigration process, we selected the adhesion molecules CD11b, active CD11b epitope, and CD62L and for the degranulation process CD203c (piecemeal degranulation marker), CD63 (degranulation marker), and CD300a (inhibitory marker of degranulation). We measured basophil responsiveness after stimulation of different activation pathways in basophils using lipopolysaccharide (LPS), peptidoglycan (PGN), formyl-methyinoyl-leucyl-phenylalanine (fMLP), and anti-FceRI-ab. Results: The expression of CD63 in basophils following activation by fMLP was significantly higher in the patient group compared to matched healthy controls, but no differences were observed after activation by anti-Fc.RI. CD300a expression was significantly higher in patients following activation by fMLP and anti-Fc.RI, and the active epitope CD11b expression was significantly higher in patients after LPS activation. In addition, we found that CD62L was not shed from the cell surface after activation with LPS and fMLP. A slight downregulation was noted after activation with anti-Fc.RI in healthy controls. Conclusion: Together, these data demonstrate that basophil functions related to adhesion and degranulation are altered in CKD patients on hemodialysis, which indicates a potential role for the basophil in the pathogenesis of complications related to infections.

Place, publisher, year, edition, pages
DUSTRI-VERLAG DR KARL FEISTLE, 2017
Keyword
chronic kidney disease, hemodialysis, basophilactivation pathways, activator markers
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-211591 (URN)10.5414/CN108992 (DOI)000405468600004 ()2-s2.0-85026763657 (Scopus ID)
Funder
Cancer and Allergy Foundation
Note

QC 20170815

Available from: 2017-08-15 Created: 2017-08-15 Last updated: 2017-08-15Bibliographically approved
Faridi, M. A., Ramachandraiah, H., Banerjee, I., Ardabli, S., Zelenin, S. & Russom, A. (2017). Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics. Journal of Nanobiotechnology, 15, Article ID 3.
Open this publication in new window or tab >>Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics
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2017 (English)In: Journal of Nanobiotechnology, ISSN 1477-3155, E-ISSN 1477-3155, Vol. 15, article id 3Article in journal (Refereed) Published
Abstract [en]

Background: Bloodstream infections (BSI) remain a major challenge with high mortality rate, with an incidence that is increasing worldwide. Early treatment with appropriate therapy can reduce BSI-related morbidity and mortality. However, despite recent progress in molecular based assays, complex sample preparation steps have become critical roadblock for a greater expansion of molecular assays. Here, we report a size based, label-free, bacteria separation from whole blood using elasto-inertial microfluidics.

Results: In elasto-inertial microfluidics, the viscoelastic flow enables size based migration of blood cells into a non- Newtonian solution, while smaller bacteria remain in the streamline of the blood sample entrance and can be sepa- rated. We first optimized the flow conditions using particles, and show continuous separation of 5 μm particles from 2 μm at a yield of 95% for 5 μm particle and 93% for 2 μm particles at respective outlets. Next, bacteria were continu- ously separated at an efficiency of 76% from undiluted whole blood sample.

Conclusion: We demonstrate separation of bacteria from undiluted while blood using elasto-inertial microfluidics. The label-free, passive bacteria preparation method has a great potential for downstream phenotypic and molecular analysis of bacteria. 

Place, publisher, year, edition, pages
BioMed Central, 2017
Keyword
Micro particle separation, Elasto-inertial microfluidics, Sepsis, Sample preparation
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-200300 (URN)10.1186/s12951-016-0235-4 (DOI)000391073000001 ()2-s2.0-85008198016 (Scopus ID)
Projects
RAPP_ID
Funder
EU, European Research Council, 115153
Note

QC 20170124

Available from: 2017-01-24 Created: 2017-01-24 Last updated: 2017-11-29Bibliographically approved
Etcheverry, S., Faridi, M. A., Ramachandraiah, H., Kumar, T., Margulis, W., Laurell, F. & Russom, A. (2017). High performance micro-flow cytometer based on optical fibres. Scientific Reports, 7, Article ID 5628.
Open this publication in new window or tab >>High performance micro-flow cytometer based on optical fibres
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, article id 5628Article in journal (Refereed) Published
Abstract [en]

Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, current microsystems remain less versatile and much slower than their large-scale counterparts. In this work, an all-silica fibre microflow cytometer is presented that measures fluorescence and scattering from particles and cells. It integrates cell transport in circular capillaries and light delivery by optical fibres. Single-stream cell focusing is performed by Elasto-inertial microfluidics to guarantee accurate and sensitive detection. The capability of this technique is extended to high flow rates (up to 800 mu l/min), enabling a throughput of 2500 particles/s. The robust, portable and low-cost system described here could be the basis for a point-of-care flow cytometer with a performance comparable to commercial systems.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-211606 (URN)10.1038/s41598-017-05843-7 (DOI)000405677200013 ()2-s2.0-85025168074 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research CouncilKnut and Alice Wallenberg FoundationSwedish Childhood Cancer Foundation
Note

QC 20170814

Available from: 2017-08-14 Created: 2017-08-14 Last updated: 2017-10-17Bibliographically approved
Zelenin, S., Ramachandraiah, H., Faridi, M. A. & Russom, A. (2017). Microfluidic-based bacteria isolation from whole blood for diagnostics of blood stream infection. In: Methods in Molecular Biology: Microchip Diagnostics. Paper presented at Methods in Molecular Biology (pp. 175-186). Springer
Open this publication in new window or tab >>Microfluidic-based bacteria isolation from whole blood for diagnostics of blood stream infection
2017 (English)In: Methods in Molecular Biology: Microchip Diagnostics, Springer, 2017, p. 175-186Conference paper, Published paper (Refereed)
Abstract [en]

Bacterial blood stream infection (BSI) potentially leads to life-threatening clinical conditions and medical emergencies such as severe sepsis, septic shock, and multi organ failure syndrome. Blood culturing is currently the gold standard for the identification of microorganisms and, although it has been automated over the decade, the process still requires 24–72 h to complete. This long turnaround time, especially for the identification of antimicrobial resistance, is driving the development of rapid molecular diagnostic methods. Rapid detection of microbial pathogens in blood related to bloodstream infections will allow the clinician to decide on or adjust the antimicrobial therapy potentially reducing the morbidity, mortality, and economic burden associated with BSI. For molecular-based methods, there is a lot to gain from an improved and straightforward method for isolation of bacteria from whole blood for downstream processing. We describe a microfluidic-based sample-preparation approach that rapidly and selectively lyses all blood cells while it extracts intact bacteria for downstream analysis. Whole blood is exposed to a mild detergent, which lyses most blood cells, and then to osmotic shock using deionized water, which eliminates the remaining white blood cells. The recovered bacteria are 100% viable, which opens up possibilities for performing drug susceptibility tests and for nucleic-acid-based molecular identification. © Springer Science+Business Media LLC 2017.

Place, publisher, year, edition, pages
Springer, 2017
Keyword
Bacteremia, Blood stream infection, Diagnostic, Microfluidics, Pathogens, Point-of-care diagnostics, Sepsis
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-202244 (URN)10.1007/978-1-4939-6734-6_14 (DOI)2-s2.0-85008466301 (Scopus ID)
Conference
Methods in Molecular Biology
Note

Funding text: This work was supported by the European Commission (projects FP7 InTopSens and IMI RAPP-ID). QC 20170222

Available from: 2017-03-06 Created: 2017-03-06 Last updated: 2017-03-06Bibliographically approved
Zhang, W., Morshed, M. M., Noyan, K., Russom, A., Sonnerborg, A. & Neogi, U. (2017). Quantitative humoral profiling of the HIV-1 proteome in elite controllers and patients with very long-term efficient antiretroviral therapy. Scientific Reports, 7, Article ID 666.
Open this publication in new window or tab >>Quantitative humoral profiling of the HIV-1 proteome in elite controllers and patients with very long-term efficient antiretroviral therapy
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, article id 666Article in journal (Refereed) Published
Abstract [en]

A major challenge in evaluating the success of HIV eradication approaches is the need for accurate measurement of persistent HIV during effective antiretroviral therapy (ART). Previous studies have reported that the anti-HIV antibody assay "luciferase immuno-precipitation systems (LIPS)"can distinguish HIV-infected individuals harboring different sizes of the viral reservoirs. We performed antibody profiling of HIV-1 proteomes using LIPS in viremic progressors (n = 38), elite controllers (ECs; n = 19) and patients with fully suppressive long-term antiretroviral therapy (ART) (n = 19) (mean 17 years). IgG was quantified against six HIV-1 fusion proteins: p24, gp41, RT, Tat, integrase and protease. Lower antibody levels to all six-fusion proteins were observed in long-term ART patients compared to viremics (p < 0.05). In contrast ECs had lower antibody levels only against Tat and Integrase (p < 0.05). Principal component analysis and cluster-network analysis identified that 68% (13/19) of the long-term ART patients clustered together with 26% (5/19) ECs. The remaining ECs clustered together with the viremics indicating non-homogeneity among the ECs. The low anti-HIV levels in the long-term treated patients may indicate a restricted remaining viral replication. In contrast, the higher levels in ECs suggest a continuous viral expression with a limited concomitant release of extracellular virus.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP, 2017
National Category
Nano Technology
Identifiers
urn:nbn:se:kth:diva-206253 (URN)10.1038/s41598-017-00759-8 (DOI)000398545400010 ()28386076 (PubMedID)
Note

QC 20170512

Available from: 2017-05-12 Created: 2017-05-12 Last updated: 2017-05-12Bibliographically approved
Banerjee, I., Salih, T., Ramachandraiah, H., Erlandsson, J., Pettersson, T., Araújo, A. C., . . . Russom, A. (2017). Slipdisc: A versatile sample preparation platform for point of care diagnostics. RSC Advances, 7(56), 35048-35054
Open this publication in new window or tab >>Slipdisc: A versatile sample preparation platform for point of care diagnostics
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2017 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 7, no 56, p. 35048-35054Article in journal (Refereed) Published
Abstract [en]

We report a microfluidic sample preparation platform called "Slipdisc" based on slipchip technology. Slipdisc is a rotational slipchip that uses a unique hand-wound clockwork mechanism for precise movement of specially fabricated polycarbonate discs. In operation, the microchannels and microchambers carved on the closely aligned microfluidic discs convert from continuous filled paths to defined compartments using the slip movement. The clockwork mechanism introduced here is characterised by a food dye experiment and a conventional HRP TMB reaction before measuring lactate dehydrogenase (LDH) enzyme levels, which is a crucial biomarker for neonatal diagnostics. The colorimetry based detection of LDH was performed with an unmodified camera and an image analysis procedure based on normalising images and observing changes in red channel intensity. The analysis showed a close to unity coefficient of determination (R2 = 0.96) in detecting the LDH concentration when compared with a standard Chemical Analyser, demonstrating the excellent performance of the slipdisc platform with colorimetric detection. The versatile point of care sample preparation platform should ideally be suited for a multitude of applications at resource-limited settings.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2017
Keyword
Chemical analysis, Chemical detection, Clocks, Colorimetric analysis, Colorimetry, Coefficient of determination, Colorimetric detection, Lactate dehydrogenase, Micro-chambers, Point of care, Point of care diagnostic, Red channels, Sample preparation, Microfluidics
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-219077 (URN)10.1039/c7ra05209j (DOI)000405811400015 ()2-s2.0-85025080747 (Scopus ID)
Note

QC 20171201

Available from: 2017-12-01 Created: 2017-12-01 Last updated: 2018-02-15Bibliographically approved
Etcheverry, S., Faridi, M. A., Ramachandraiah, H., Margulis, W., Laurell, F. & Russom, A. (2016). All fiber based micro-flow cytometer by combining optical fiber with inertial focusing. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016: . Paper presented at 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016 (pp. 1655-1656). Chemical and Biological Microsystems Society
Open this publication in new window or tab >>All fiber based micro-flow cytometer by combining optical fiber with inertial focusing
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2016 (English)In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society , 2016, p. 1655-1656Conference paper, Published paper (Refereed)
Abstract [en]

Towards a portable point of care flow cytometry platform, we present here an integrated all optical fiber-based optofluidic system capable of counting and discriminating fluorescent particles and cells. The robust and compact device incorporates optical fibers and circular capillaries to build an all-fiber optofluidic device to enable counting particles based on their fluorescent and back-scatter light emission. Here, we combine this with inertial- and elasto-inertial microfluidics for sheathless particle and cell focusing for integrated detection with scattering and fluorescence detections - all necessary components of standard cytometers. We validated the system for cell counting based on scattering and fluorescence.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society, 2016
Keyword
Cell counting, Flow cytometry, Inertial focusing, Optical fibers
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-207530 (URN)2-s2.0-85014148814 (Scopus ID)9780979806490 (ISBN)
Conference
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016
Note

QC 20170531

Available from: 2017-05-31 Created: 2017-05-31 Last updated: 2018-02-27Bibliographically approved
Faridi, M. A., Ramachandraiah, H., Iranmanesh, I. S., Grishenkov, D., Wiklund, M. & Russom, A. (2016). Microbubble assisted cell sorting by acoustophoresis. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016: . Paper presented at 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016 (pp. 1677-1678). Chemical and Biological Microsystems Society
Open this publication in new window or tab >>Microbubble assisted cell sorting by acoustophoresis
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2016 (English)In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society , 2016, p. 1677-1678Conference paper, Published paper (Refereed)
Abstract [en]

Polymer shelled gas microbubbles (MBs) are used to sort cells in a microfluidic chip under acoustic standing waves (SW). When particles are subjected to SW based on their acoustic contrast factor (ACF) they migrate to nodes (positive contrast factor particles; PACP) or antinodes (negative acoustic contrast particles; NACP)[1]. We have bounded functionalized MBs with cells such that, they can be selectively migrated to antinodes under SW and sorted from unbounded cell both in no flow and flow conditions. Here we demonstrate acoustic mediated microbubble tagged cell sorting with 75% efficiency.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society, 2016
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-207568 (URN)2-s2.0-85014178442 (Scopus ID)9780979806490 (ISBN)
Conference
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016
Note

Conference code: 126047; Export Date: 22 May 2017; Conference Paper; Correspondence Address: Faridi, M.A.; School of Biotechnology, Royal Institute of Technology KTHSweden; email: mafaridi@kth.se. QC 20170530

Available from: 2017-05-30 Created: 2017-05-30 Last updated: 2017-05-30Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0002-0242-358X

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