Open this publication in new window or tab >>MSF Epicentre Mbarara Research Centre, Mbarara, Uganda.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Nano Biotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Department of Global Public Health, Karolinska Institutet, Stockholm, Sweden, Stockholm.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden, Stockholm.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden, Stockholm.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Nano Biotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
MSF Epicentre Mbarara Research Centre, Mbarara, Uganda; Department of Paediatrics and Child Health, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden, Stockholm.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Nano Biotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Department of Paediatrics and Child Health, Faculty of Medicine, Mbarara University of Science and Technology, Mbarara, Uganda.
Department of Global Public Health, Karolinska Institutet, Stockholm, Sweden, Stockholm; Sachs' Children & Youth Hospital, South General Hospital, Stockholm, Sweden, Stockholm.
Institut Pasteur of Bangui, Bangui, Central Africa Republic, Institut Pasteur of Bangui, Bangui, Central Africa Republic.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Nano Biotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Show others...
2024 (English)In: Talanta Open, E-ISSN 2666-8319, Vol. 10, article id 100357Article in journal (Refereed) Published
Abstract [en]
Objectives: Meningitis is a medical emergency, and it is crucial to diagnose it accurately and promptly in order to manage patients effectively. It would, therefore, be essential to introduce and have fast, accurate, and user-friendly methods to determine the cause of these infections. This study aimed to demonstrate a potentially cost-effective new approach for detecting meningitis using a paper-based vertical flow microarray, which could be useful in settings with limited resources. Methods: We describe a multiplex paper microarray for detecting Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, and Salmonella spp. by the passive vertical flow of PCR-amplified clinical samples. A multibiotinylated amplicon was obtained as a product of PCR in the presence of both a biotinylated primer and biotin-11-dUTP. An enhancement step based on an enzyme-free gold enhancement protocol was also used to facilitate visual detection. Results: This study showed that the vertical flow microarray (previously evaluated for one pathogen) can discriminately detect the amplification results down to the 102 copies of DNA limit for four meningitis pathogens in a multiplexed set-up. The study further demonstrated the ability of this device and setup to detect three of the four pathogens from clinical biosamples. Discussion: This study demonstrated the capacity of a vertical flow microarray device to detect amplification products for four prevalent meningitis pathogens in a multiplex format. The vertical flow microarray demonstrated consistent visualization of the expected gene amplification results; however, indicating limitations in the pre- and amplification steps. This study highlights the potential of this multiplexing method for diagnosing meningitis and other syndromic diseases caused by various pathogens, especially in resource-limited areas.
Place, publisher, year, edition, pages
Elsevier BV, 2024
Keywords
Global health, Low-resource settings, Multiplex paper microarray, Paediatrics, Passive vertical flow, Point-of-care, Signal enhancement
National Category
Medical and Health Sciences
Identifiers
urn:nbn:se:kth:diva-353912 (URN)10.1016/j.talo.2024.100357 (DOI)001318148500001 ()2-s2.0-85204042669 (Scopus ID)
Note
QC 20241009
2024-09-252024-09-252025-03-13Bibliographically approved