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Faridi, Muhammad AsimORCID iD iconorcid.org/0000-0003-1176-0905
Publications (5 of 5) Show all publications
Faridi, M. A., Shahzad, A. F., Russom, A. & Wiklund, M. (2018). Milliliter scale acoustophoresis based bioparticle processing platform. In: ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018: . Paper presented at ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018, Dubrovnik, Croatia, 10 June 2018 through 13 June 2018. ASME Press
Open this publication in new window or tab >>Milliliter scale acoustophoresis based bioparticle processing platform
2018 (English)In: ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018, ASME Press, 2018Conference paper, Published paper (Refereed)
Abstract [en]

Bioparticles such as mammalian cells and bacteria can be manipulated directly or indirectly for multiple applications such as sample preparation for diagnostic applications mainly up-concentration, enrichment & separation as well as immunoassay development. There are various active and passive microfluidic particle manipulation techniques where Acoustophoresis is a powerful technique showing high cell viability. The use of disposable glass capillaries for acoustophoresis, instead of cleanroom fabricated glass-silicon chip can potentially bring down the cost factor substantially, aiding the realization of this technique for real-world diagnostic devices. Unlike available chips and capillary-based microfluidic devices, we report milliliter-scale platform able to accommodate 1ml of a sample for acoustophoresis based processing on a market available glass capillary. Although it is presented as a generic platform but as a demonstration we have shown that polystyrene suspending medium sample can be processed with trapping efficiency of 87% and the up-concentration factor of 10 times in a flow through manner i.e., at 35µl/min. For stationary volume accommodation, this platform practically offers 50 times more sample handling capacity than most of the microfluidic setups. Furthermore, we have also shown that with diluted blood (0.6%) in a flow-through manner, 82% of the white blood cells (WBCs) per ml could be kept trapped. This milliliter platform could potentially be utilized for assisting in sample preparation, plasma separation as well as a flow-through immunoassay assay development for clinical diagnostic applications.

Place, publisher, year, edition, pages
ASME Press, 2018
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:kth:diva-238419 (URN)2-s2.0-85053923284 (Scopus ID)9780791851197 (ISBN)
Conference
ASME 2018 16th International Conference on Nanochannels, Microchannels, and Minichannels, ICNMM 2018, Dubrovnik, Croatia, 10 June 2018 through 13 June 2018
Note

QC 20181108

Available from: 2018-11-08 Created: 2018-11-08 Last updated: 2018-11-08Bibliographically approved
Faridi, M. A., Ramachandraiah, H., Banerjee, I., Ardabli, S., Zelenin, S. & Russom, A. (2017). Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics. Journal of Nanobiotechnology, 15, Article ID 3.
Open this publication in new window or tab >>Elasto-inertial microfluidics for bacteria separation from whole blood for sepsis diagnostics
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2017 (English)In: Journal of Nanobiotechnology, ISSN 1477-3155, E-ISSN 1477-3155, Vol. 15, article id 3Article in journal (Refereed) Published
Abstract [en]

Background: Bloodstream infections (BSI) remain a major challenge with high mortality rate, with an incidence that is increasing worldwide. Early treatment with appropriate therapy can reduce BSI-related morbidity and mortality. However, despite recent progress in molecular based assays, complex sample preparation steps have become critical roadblock for a greater expansion of molecular assays. Here, we report a size based, label-free, bacteria separation from whole blood using elasto-inertial microfluidics.

Results: In elasto-inertial microfluidics, the viscoelastic flow enables size based migration of blood cells into a non- Newtonian solution, while smaller bacteria remain in the streamline of the blood sample entrance and can be sepa- rated. We first optimized the flow conditions using particles, and show continuous separation of 5 μm particles from 2 μm at a yield of 95% for 5 μm particle and 93% for 2 μm particles at respective outlets. Next, bacteria were continu- ously separated at an efficiency of 76% from undiluted whole blood sample.

Conclusion: We demonstrate separation of bacteria from undiluted while blood using elasto-inertial microfluidics. The label-free, passive bacteria preparation method has a great potential for downstream phenotypic and molecular analysis of bacteria. 

Place, publisher, year, edition, pages
BioMed Central, 2017
Keywords
Micro particle separation, Elasto-inertial microfluidics, Sepsis, Sample preparation
National Category
Medical Biotechnology
Identifiers
urn:nbn:se:kth:diva-200300 (URN)10.1186/s12951-016-0235-4 (DOI)000391073000001 ()2-s2.0-85008198016 (Scopus ID)
Projects
RAPP_ID
Funder
EU, European Research Council, 115153
Note

QC 20170124

Available from: 2017-01-24 Created: 2017-01-24 Last updated: 2019-02-28Bibliographically approved
Etcheverry, S., Faridi, M. A., Ramachandraiah, H., Kumar, T., Margulis, W., Laurell, F. & Russom, A. (2017). High performance micro-flow cytometer based on optical fibres. Scientific Reports, 7, Article ID 5628.
Open this publication in new window or tab >>High performance micro-flow cytometer based on optical fibres
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, article id 5628Article in journal (Refereed) Published
Abstract [en]

Flow cytometry is currently the gold standard for analysis of cells in the medical laboratory and biomedical research. Fuelled by the need of point-of-care diagnosis, a significant effort has been made to miniaturize and reduce cost of flow cytometers. However, despite recent advances, current microsystems remain less versatile and much slower than their large-scale counterparts. In this work, an all-silica fibre microflow cytometer is presented that measures fluorescence and scattering from particles and cells. It integrates cell transport in circular capillaries and light delivery by optical fibres. Single-stream cell focusing is performed by Elasto-inertial microfluidics to guarantee accurate and sensitive detection. The capability of this technique is extended to high flow rates (up to 800 mu l/min), enabling a throughput of 2500 particles/s. The robust, portable and low-cost system described here could be the basis for a point-of-care flow cytometer with a performance comparable to commercial systems.

Place, publisher, year, edition, pages
Nature Publishing Group, 2017
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-211606 (URN)10.1038/s41598-017-05843-7 (DOI)000405677200013 ()2-s2.0-85025168074 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscienceSwedish Research CouncilKnut and Alice Wallenberg FoundationSwedish Childhood Cancer Foundation
Note

QC 20170814

Available from: 2017-08-14 Created: 2017-08-14 Last updated: 2017-10-17Bibliographically approved
Zelenin, S., Ramachandraiah, H., Faridi, M. A. & Russom, A. (2017). Microfluidic-based bacteria isolation from whole blood for diagnostics of blood stream infection. In: Methods in Molecular Biology: Microchip Diagnostics. Paper presented at Methods in Molecular Biology (pp. 175-186). Springer
Open this publication in new window or tab >>Microfluidic-based bacteria isolation from whole blood for diagnostics of blood stream infection
2017 (English)In: Methods in Molecular Biology: Microchip Diagnostics, Springer, 2017, p. 175-186Conference paper, Published paper (Refereed)
Abstract [en]

Bacterial blood stream infection (BSI) potentially leads to life-threatening clinical conditions and medical emergencies such as severe sepsis, septic shock, and multi organ failure syndrome. Blood culturing is currently the gold standard for the identification of microorganisms and, although it has been automated over the decade, the process still requires 24–72 h to complete. This long turnaround time, especially for the identification of antimicrobial resistance, is driving the development of rapid molecular diagnostic methods. Rapid detection of microbial pathogens in blood related to bloodstream infections will allow the clinician to decide on or adjust the antimicrobial therapy potentially reducing the morbidity, mortality, and economic burden associated with BSI. For molecular-based methods, there is a lot to gain from an improved and straightforward method for isolation of bacteria from whole blood for downstream processing. We describe a microfluidic-based sample-preparation approach that rapidly and selectively lyses all blood cells while it extracts intact bacteria for downstream analysis. Whole blood is exposed to a mild detergent, which lyses most blood cells, and then to osmotic shock using deionized water, which eliminates the remaining white blood cells. The recovered bacteria are 100% viable, which opens up possibilities for performing drug susceptibility tests and for nucleic-acid-based molecular identification. © Springer Science+Business Media LLC 2017.

Place, publisher, year, edition, pages
Springer, 2017
Keywords
Bacteremia, Blood stream infection, Diagnostic, Microfluidics, Pathogens, Point-of-care diagnostics, Sepsis
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-202244 (URN)10.1007/978-1-4939-6734-6_14 (DOI)2-s2.0-85008466301 (Scopus ID)
Conference
Methods in Molecular Biology
Note

Funding text: This work was supported by the European Commission (projects FP7 InTopSens and IMI RAPP-ID). QC 20170222

Available from: 2017-03-06 Created: 2017-03-06 Last updated: 2017-03-06Bibliographically approved
Etcheverry, S., Faridi, M. A., Ramachandraiah, H., Margulis, W., Laurell, F. & Russom, A. (2016). All fiber based micro-flow cytometer by combining optical fiber with inertial focusing. In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016: . Paper presented at 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016 (pp. 1655-1656). Chemical and Biological Microsystems Society
Open this publication in new window or tab >>All fiber based micro-flow cytometer by combining optical fiber with inertial focusing
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2016 (English)In: 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society , 2016, p. 1655-1656Conference paper, Published paper (Refereed)
Abstract [en]

Towards a portable point of care flow cytometry platform, we present here an integrated all optical fiber-based optofluidic system capable of counting and discriminating fluorescent particles and cells. The robust and compact device incorporates optical fibers and circular capillaries to build an all-fiber optofluidic device to enable counting particles based on their fluorescent and back-scatter light emission. Here, we combine this with inertial- and elasto-inertial microfluidics for sheathless particle and cell focusing for integrated detection with scattering and fluorescence detections - all necessary components of standard cytometers. We validated the system for cell counting based on scattering and fluorescence.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society, 2016
Keywords
Cell counting, Flow cytometry, Inertial focusing, Optical fibers
National Category
Physical Sciences
Identifiers
urn:nbn:se:kth:diva-207530 (URN)2-s2.0-85014148814 (Scopus ID)9780979806490 (ISBN)
Conference
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, 9 October 2016 through 13 October 2016
Note

QC 20170531

Available from: 2017-05-31 Created: 2017-05-31 Last updated: 2018-02-27Bibliographically approved
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ORCID iD: ORCID iD iconorcid.org/0000-0003-1176-0905

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