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Publications (6 of 6) Show all publications
Thul, P. J., Åkesson, L., Wiking, M., Mahdessian, D., Geladaki, A., Ait Blal, H., . . . Lundberg, E. (2017). A subcellular map of the human proteome. Science, 356(6340), Article ID 820.
Open this publication in new window or tab >>A subcellular map of the human proteome
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2017 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 356, no 6340, article id 820Article in journal (Refereed) Published
Abstract [en]

Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.

Place, publisher, year, edition, pages
American Association for the Advancement of Science, 2017
Keywords
antibody, proteome, biology, cells and cell components, disease incidence, image analysis, physiological response, protein, proteomics, spatial distribution, Article, cell organelle, cellular distribution, human, human cell, immunofluorescence microscopy, mass spectrometry, priority journal, protein analysis, protein localization, protein protein interaction, single cell analysis, transcriptomics
National Category
Cell Biology
Identifiers
urn:nbn:se:kth:diva-216588 (URN)10.1126/science.aal3321 (DOI)000401957900032 ()2-s2.0-85019201137 (Scopus ID)
Note

QC 20171208

Available from: 2017-12-08 Created: 2017-12-08 Last updated: 2019-10-03Bibliographically approved
Alm, T. L., von Feilitzen, K. & Uhlén, M. (2016). ANTIBODYPEDIA: THE WIKI OF ANTIBODIES. Paper presented at Annual Meeting of the American-Society-for-Cell-Biology (ASCB), DEC 03-07, 2016, San Francisco, CA. Molecular Biology of the Cell, 27
Open this publication in new window or tab >>ANTIBODYPEDIA: THE WIKI OF ANTIBODIES
2016 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 27Article in journal (Refereed) Published
Place, publisher, year, edition, pages
AMER SOC CELL BIOLOGY, 2016
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-204763 (URN)000396046900059 ()
Conference
Annual Meeting of the American-Society-for-Cell-Biology (ASCB), DEC 03-07, 2016, San Francisco, CA
Note

QC 20170529

Available from: 2017-05-29 Created: 2017-05-29 Last updated: 2017-05-29Bibliographically approved
Bjork, L., Ait Blal, C., Alm, T. L., Bäckström, A., Gnann, C., Hjelmare, M., . . . Lundberg, E. (2016). Application specific antibody validation. The Human Protein Atlas validation scheme and how to confirm subcellular protein localization.. Paper presented at Annual Meeting of the American-Society-for-Cell-Biology (ASCB), DEC 03-07, 2016, San Francisco, CA. Molecular Biology of the Cell, 27
Open this publication in new window or tab >>Application specific antibody validation. The Human Protein Atlas validation scheme and how to confirm subcellular protein localization.
Show others...
2016 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 27Article in journal (Refereed) Published
Place, publisher, year, edition, pages
AMER SOC CELL BIOLOGY, 2016
National Category
Cell Biology
Identifiers
urn:nbn:se:kth:diva-205179 (URN)000396047100677 ()
Conference
Annual Meeting of the American-Society-for-Cell-Biology (ASCB), DEC 03-07, 2016, San Francisco, CA
Note

QC 20170410

Available from: 2017-04-10 Created: 2017-04-10 Last updated: 2017-11-29Bibliographically approved
Alm, T. L., Lundberg, E. & Uhlén, M. (2016). The Affinity Binder Knockdown Initiative.. Paper presented at Annual Meeting of the American-Society-for-Cell-Biology (ASCB), DEC 03-07, 2016, San Francisco, CA. Molecular Biology of the Cell, 27
Open this publication in new window or tab >>The Affinity Binder Knockdown Initiative.
2016 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 27Article in journal (Refereed) Published
Place, publisher, year, edition, pages
AMER SOC CELL BIOLOGY, 2016
National Category
Cell Biology
Identifiers
urn:nbn:se:kth:diva-205178 (URN)000396047100685 ()
Conference
Annual Meeting of the American-Society-for-Cell-Biology (ASCB), DEC 03-07, 2016, San Francisco, CA
Note

QC 20170410

Available from: 2017-04-10 Created: 2017-04-10 Last updated: 2017-11-29Bibliographically approved
Alm, T. L., von Feilitzen, K. & Uhlén, M. (2015). Antibodypedia - The wiki of antibodies. Molecular Biology of the Cell, 26
Open this publication in new window or tab >>Antibodypedia - The wiki of antibodies
2015 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 26Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
AMER SOC CELL BIOLOGY, 2015
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-242776 (URN)000209928600548 ()
Note

QC 20190208

Available from: 2019-02-08 Created: 2019-02-08 Last updated: 2019-08-21Bibliographically approved
Alm, T. L., Lundberg, E. & Uhlén, M. (2015). The Affinity Binder Knockdown Initiative. Molecular Biology of the Cell, 26
Open this publication in new window or tab >>The Affinity Binder Knockdown Initiative
2015 (English)In: Molecular Biology of the Cell, ISSN 1059-1524, E-ISSN 1939-4586, Vol. 26Article in journal, Meeting abstract (Other academic) Published
Place, publisher, year, edition, pages
AMER SOC CELL BIOLOGY, 2015
National Category
Cell and Molecular Biology
Identifiers
urn:nbn:se:kth:diva-242777 (URN)000209928600549 ()
Note

QC 20190208

Available from: 2019-02-08 Created: 2019-02-08 Last updated: 2019-08-21Bibliographically approved
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Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0002-2643-8241

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