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Ohlander, Anna
Publications (4 of 4) Show all publications
Bose, I., Ohlander, A., Kutter, C. & Russom, A. (2018). An integrated all foil based micro device for point of care diagnostic applications. Sensors and actuators. B, Chemical, 259, 917-925
Open this publication in new window or tab >>An integrated all foil based micro device for point of care diagnostic applications
2018 (English)In: Sensors and actuators. B, Chemical, ISSN 0925-4005, E-ISSN 1873-3077, Vol. 259, p. 917-925Article in journal (Refereed) Published
Abstract [en]

Point-of-Care (POC) diagnostics often fail to meet the market requirements of low cost and advanced functionality, and are often limited to lateral flow based serological diagnostics with reduced sensitivity and specificity. We report here on an integrated microfluidic absorbance measurement device fabricated by roll-to-roll (R2R) compatible manufacturing processes, suitable for low cost POC systems. It is a device exclusively made of foils and takes external light from a low cost LED and converts the point light source to a homogeneous light via a foil based optical filter at the bottom of the device. The light is converted to an electrical signal by an amorphous organic semiconductor (OSC) material, integrated with screen-printed carbon finger on top of the device for electrical measurement. As a proof of principle, we demonstrate DNA hybridization assay, where the target DNA is coupled to magnetic beads for absorbance measurement. The device successfully distinguishes between matched and mismatched DNA hybridization and can differentiate between 1 μM, 50 nM and 2.5 nM DNA target concentrations. The inherent characteristics of the substrates and R2R fabrication concept significantly reduce the cost, making it suitable for POC applications at resource-limited settings. 

Place, publisher, year, edition, pages
Elsevier B.V., 2018
Keywords
DNA analysis, Microfluidics, Optical detection, Point-of-care, Amorphous carbon, Carbon, Costs, DNA, Light sources, Organic light emitting diodes (OLED), Substrates, Absorbance measurements, Dna hybridization assays, Inherent characteristics, Integrated microfluidics, Point of care, Point of care diagnostic, Cost reduction
National Category
Biological Sciences Chemical Sciences
Identifiers
urn:nbn:se:kth:diva-223112 (URN)10.1016/j.snb.2017.12.133 (DOI)000424877600111 ()2-s2.0-85039735667 (Scopus ID)
Note

Export Date: 13 February 2018; Article; CODEN: SABCE; Correspondence Address: Russom, A.; Div of Proteomics and Nanobiotechnology, Science for Life Laboratory, KTH Royal Institute of TechnologySweden; email: aman.russom@scilifelab.se. QC 20180327

Available from: 2018-03-27 Created: 2018-03-27 Last updated: 2018-03-27Bibliographically approved
Banerjee, I., Salih, T., Ohlander, A. & Russom, A. (2017). Three dimensional Slipdisc aimed at HIV viral load detection. In: : . Paper presented at HIV and Hepatitis Nordic Conference, 2017 (September 27-29), Stockholm, Sweden. (Poster).
Open this publication in new window or tab >>Three dimensional Slipdisc aimed at HIV viral load detection
2017 (English)Conference paper, Poster (with or without abstract) (Refereed)
Abstract [en]

In this study, we introduce a three dimensional Slipdisc1 based on slipchip technology, aimed to be used at detection of HIV-1 viral load in resource limited settings. This method is based on magnetic bead based isolation of RNA from sample, followed by release of RNA in an elution buffer, followed by amplification of the initial concentration of RNA in an elution buffer. The novelty is the extraction method which is a one step method and involves using magnetic beads attached to sample DNA to pass through an immiscible oil phase2 for one step washing.

National Category
Engineering and Technology
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-255526 (URN)
Conference
HIV and Hepatitis Nordic Conference, 2017 (September 27-29), Stockholm, Sweden. (Poster)
Note

QC 20190819

Available from: 2019-07-30 Created: 2019-07-30 Last updated: 2019-08-19Bibliographically approved
Ohlander, A., Bose, I., Njenda, D., Zelenin, S., Neogi, U., Kutter, C. & Russom, A.Lab-on-foil based portable microPCR for point-of-care nucleic acid testing of HIV-1.
Open this publication in new window or tab >>Lab-on-foil based portable microPCR for point-of-care nucleic acid testing of HIV-1
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(English)Manuscript (preprint) (Other academic)
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:kth:diva-205728 (URN)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20170427

Available from: 2017-04-24 Created: 2017-04-24 Last updated: 2017-05-29Bibliographically approved
Ohlander, A., Zelenin, S., Huygens, F., Kutter, C. & Russom, A.Staphylococcus Aureus subtyping and detection of MRSA on a microfluidic lab-on-Foil device.
Open this publication in new window or tab >>Staphylococcus Aureus subtyping and detection of MRSA on a microfluidic lab-on-Foil device
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(English)Manuscript (preprint) (Other academic)
National Category
Biomedical Laboratory Science/Technology
Identifiers
urn:nbn:se:kth:diva-205726 (URN)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20170427

Available from: 2017-04-24 Created: 2017-04-24 Last updated: 2017-05-29Bibliographically approved
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