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Publications (6 of 6) Show all publications
Reu, P., Svedberg, G., Hässler, L., Möller, B., Svahn Andersson, H. & Gantelius, J. (2019). A 61% lighter cell culture dish to reduce plastic waste. PLoS ONE, 14(4), Article ID e0216251.
Open this publication in new window or tab >>A 61% lighter cell culture dish to reduce plastic waste
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2019 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 14, no 4, article id e0216251Article in journal (Refereed) Published
Abstract [en]

Cell culture is a ubiquitous and flexible research method. However, it heavily relies on plastic consumables generating millions of tonnes of plastic waste yearly. Plastic waste is a major and growing global concern. Here we describe a new cell culture dish that offers a culture area equivalent to three petri dishes but that is on average 61% lighter and occupies 67% less volume. Our dish is composed of a lid and three thin containers surrounded by a light outer shell. Cell culture can be performed in each of the containers sequentially. The outer shell provides the appropriate structure for the manipulation of the dish as a whole. The prototype was tested by sequentially growing cells in each of its containers. As a control, sequential cultures in groups of 3 petri dishes were performed. No statistical differences were found between the prototype and the control in terms of cell number, cell viability or cell distribution.

Place, publisher, year, edition, pages
Public Library of Science, 2019
National Category
Environmental Engineering
Identifiers
urn:nbn:se:kth:diva-258015 (URN)10.1371/journal.pone.0216251 (DOI)000466364800040 ()31039189 (PubMedID)2-s2.0-85065404122 (Scopus ID)
Note

QC 20191004

Available from: 2019-09-09 Created: 2019-09-09 Last updated: 2019-10-07Bibliographically approved
Svedberg, G. (2019). Novel planar and particle-based microarrays for point-of-care diagnostics. (Doctoral dissertation). Stockholm: KTH Royal Institute of Technology
Open this publication in new window or tab >>Novel planar and particle-based microarrays for point-of-care diagnostics
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Point-of-care assays are easy-to-use, portable and inexpensive tests that can

be used to aid diagnostics by measuring levels of disease-specific molecules

in settings where access to advanced laboratory equipment and trained

personnel are limited, such as at the patient's bedside or in low resource

parts of developing countries. In order to achieve high multiplexing

capacities, such assays can be based on planar microarrays consisting of

spots immobilized on a flat surface or on particle-based microarrays based

on populations of encoded particles. The aim of the work presented in this

thesis is to develop new point-of-care amenable planar and particle-based

microarrays that allow for highly multiplexed assays while maintaining low

sample-to-result times, complexity and instrumentation requirements.

Paper I demonstrates the use graphically encoded particles for colorimetric

detection of autoantibodies using a consumer-grade flatbed scanner. Four

graphical characters on the surface of each particle allows for millions of

codes and the use of gold nanoparticles as a detection label allows both the

code and the signal intensity to be read out in a single image.

Paper II describes a signal enhancement method that increases the

sensitivity of gold nanoparticle detection on planar microarrays. Using this

method, detection of allergen-specific IgE can be carried out using a

consumer-grade flatbed scanner instead of a more expensive fluorescence

scanner without sacrificing assay performance.

Paper III demonstrates the use of an isothermal DNA amplification method

for detection of adenoviral DNA on a paper-based microarray. Using an

isothermal amplification method eliminates the need for a thermocycler,

reducing the instrumentation required for such detection.

Paper IV shows the use of solid-phase PCR to amplify bacterial DNA directly

on the surface of particles. This strategy reduces assay time by eliminating

the need for separate amplification and hybridisation steps.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2019. p. 88
Series
TRITA-CBH-FOU ; 2019:13
Keywords
Planar microarrays, Particle-based microarrays, Point-of-care diagnostics, Colorimetric detection, Signal enhancement, Isothermal DNA amplification, Solid-phase DNA amplification
National Category
Medical Biotechnology
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-244430 (URN)978-91-7873-101-5 (ISBN)
Public defence
2019-03-22, Science for Life Laboratory, room Air & Fire, Tomtebodavägen 23A, Solna, 10:00 (English)
Opponent
Supervisors
Note

QC 20190221

Available from: 2019-02-21 Created: 2019-02-20 Last updated: 2019-02-21Bibliographically approved
Dias, J. T., Svedberg, G., Nystrand, M., Svahn Andersson, H. & Gantelius, J. (2018). Rapid signal enhancement method for nanoprobe-based biosensing (vol 7, 2017). Scientific Reports, 8(1), Article ID 8184.
Open this publication in new window or tab >>Rapid signal enhancement method for nanoprobe-based biosensing (vol 7, 2017)
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2018 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, no 1, article id 8184Article in journal (Refereed) Published
Abstract [en]

In the Methods section of this Article references 18 to 22 are incorrectly cited. The correct references were omitted from the reference list and appear below as references 1-5. References 18 to 22 are correctly cited in Introduction and Results and Discussion sections. "AuNPs of 10 nm in diameter were prepared following the protocol described by Bastus et al.18." should read: "AuNPs of 10 nm in diameter were prepared following the protocol described by Bastus et al.1." "AgNPs of 90 nm in diameter were prepared following the protocol described by Rivero et al.19." should read: "AgNPs of 90 nm in diameter were prepared following the protocol described by Rivero et al.2" "The size was determined by UV-Vis spectroscopy according to the AgNPs size theory demonstrated by Malynych20." should read: "The size was determined by UV-Vis spectroscopy according to the AgNPs size theory demonstrated by Malynych3." "The coupling of antibody to the NPs was prepared following a modified version of a protocol previously reported by Puertas et al.21." should read: "The coupling of antibody to the NPs was prepared following a modified version of a protocol previously reported by Puertas et al.4." "Microarrays were prepared as previously reported by our group22." should read: "Microarrays were prepared as previously reported by our group5.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP, 2018
National Category
Organic Chemistry
Identifiers
urn:nbn:se:kth:diva-230435 (URN)10.1038/s41598-018-26155-4 (DOI)000432657600002 ()29786686 (PubMedID)2-s2.0-85047421736 (Scopus ID)
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20180615

Available from: 2018-06-15 Created: 2018-06-15 Last updated: 2019-10-10Bibliographically approved
Dias, J. T., Svedberg, G., Nystrand, M., Andersson-Svahn, H. & Gantelius, J. (2017). Rapid signal enhancement method for nanoprobe-based biosensing. Scientific Reports, 7, Article ID 6837.
Open this publication in new window or tab >>Rapid signal enhancement method for nanoprobe-based biosensing
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2017 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 7, article id 6837Article in journal (Refereed) Published
Abstract [en]

The introduction of nanomaterials as detection reagents has enabled improved sensitivity and facilitated detection in a variety of bioanalytical assays. However, high nanoprobe densities are typically needed for colorimetric detection and to circumvent this limitation several enhancement protocols have been reported. Nevertheless, there is currently a lack of universal, enzyme-free and versatile methods that can be readily applied to existing as well as new biosensing strategies. The novel method presented here is shown to enhance the signal of gold nanoparticles enabling visual detection of a spot containing < 10 nanoparticles. Detection of Protein G on paper arrays was improved by a 100-fold amplification factor in under five minutes of assay time, using IgG-labelled gold, silver, silica and iron oxide nanoprobes. Furthermore, we show that the presented protocol can be applied to a commercial allergen microarray assay, ImmunoCAP ISAC sIgE 112, attaining a good agreement with fluorescent detection when analysing human clinical samples.

Place, publisher, year, edition, pages
NATURE PUBLISHING GROUP, 2017
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:kth:diva-212341 (URN)10.1038/s41598-017-07030-0 (DOI)000406610000088 ()2-s2.0-85026428495 (Scopus ID)
Note

QC 20170823

Available from: 2017-08-23 Created: 2017-08-23 Last updated: 2019-02-20Bibliographically approved
Svedberg, G., Jeong, Y., Na, H., Jang, J., Nilsson, P., Kwon, S., . . . Svahn Andersson, H. (2017). Towards encoded particles for highly multiplexed colorimetric point of care autoantibody detection. Lab on a Chip, 17(3), 549-556
Open this publication in new window or tab >>Towards encoded particles for highly multiplexed colorimetric point of care autoantibody detection
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2017 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 17, no 3, p. 549-556Article in journal (Refereed) Published
Abstract [en]

Highly multiplexed point of care tests could improve diagnostic accuracy and differential diagnostic capacity in for instance emergency medicine and low resource environments. Available technology platforms for POC biomarker detection are typically simplex or low-plexed, whereas common lab-based microarray systems allow for the simultaneous detection of thousands of DNA or protein biomarkers. In this study, we demonstrate a novel suspension particle array platform that utilizes 900 mu m bricks for POC amenable colorimetric biomarker detection with an encoding capacity of over two million. Due to the mm-scale size, both the lithographic codes and colorimetric signals of individual particles can be visualized using a consumer grade office flatbed scanner, with a potential for simultaneous imaging of around 19000 particles per scan. The analytical sensitivity of the assay was determined to be 4 ng ml(-1) using an antibody model system. As a proof of concept, autoantibodies toward anoctamin 2 were detected in order to discriminate between multiple sclerosis plasma samples and healthy controls with p < 0.0001 and an inter-assay % CV of 9.44%.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2017
National Category
Biological Sciences
Identifiers
urn:nbn:se:kth:diva-204746 (URN)10.1039/c6lc01358a (DOI)000395887900019 ()28102419 (PubMedID)2-s2.0-85010943844 (Scopus ID)
Funder
VINNOVAEU, FP7, Seventh Framework Programme, 615458
Note

QC 20170601

Available from: 2017-06-01 Created: 2017-06-01 Last updated: 2019-02-20Bibliographically approved
Jeong, Y., Svedberg, G., Réu, P., Lee, Y., Song, S. W., Na, H., . . . Kwon, S.Solid-phase PCR on graphically encoded microparticles for multiplexed colorimetric detection of bacterial DNA.
Open this publication in new window or tab >>Solid-phase PCR on graphically encoded microparticles for multiplexed colorimetric detection of bacterial DNA
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(English)Manuscript (preprint) (Other academic)
National Category
Medical Biotechnology
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-244428 (URN)
Note

QC 20190221

Available from: 2019-02-20 Created: 2019-02-20 Last updated: 2019-02-21Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0003-4727-6138

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