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Sjöqvist, Conny
Publications (2 of 2) Show all publications
Wampach, L., Heintz-Buschart, A., Fritz, J. V., Ramiro-Garcia, J., Habier, J., Herold, M., . . . Wilmes, P. (2018). Birth mode is associated with earliest strain-conferred gut microbiome functions and immunostimulatory potential. Nature Communications, 9(1), Article ID 5091.
Open this publication in new window or tab >>Birth mode is associated with earliest strain-conferred gut microbiome functions and immunostimulatory potential
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2018 (English)In: Nature Communications, ISSN 2041-1723, E-ISSN 2041-1723, Vol. 9, no 1, article id 5091Article in journal (Refereed) Published
Abstract [en]

The rate of caesarean section delivery (CSD) is increasing worldwide. It remains unclear whether disruption of mother-to-neonate transmission of microbiota through CSD occurs and whether it affects human physiology. Here we perform metagenomic analysis of earliest gut microbial community structures and functions. We identify differences in encoded functions between microbiomes of vaginally delivered (VD) and CSD neonates. Several functional pathways are over-represented in VD neonates, including lipopolysaccharide (LPS) biosynthesis. We link these enriched functions to individual-specific strains, which are transmitted from mothers to neonates in case of VD. The stimulation of primary human immune cells with LPS isolated from early stool samples of VD neonates results in higher levels of tumour necrosis factor (TNF-α) and interleukin 18 (IL-18). Accordingly, the observed levels of TNF-α and IL-18 in neonatal blood plasma are higher after VD. Taken together, our results support that CSD disrupts mother-to-neonate transmission of specific microbial strains, linked functional repertoires and immune-stimulatory potential during a critical window for neonatal immune system priming.

Place, publisher, year, edition, pages
Nature Publishing Group, 2018
Keywords
beta interferon, galectin 1, gamma interferon, granzyme B, interleukin 10, interleukin 12, interleukin 13, interleukin 15, interleukin 18, interleukin 1beta, interleukin 2, interleukin 21, interleukin 23, interleukin 23p40, interleukin 27, interleukin 4, interleukin 5, interleukin 6, interleukin 8, RNA 16S, tumor necrosis factor, unclassified drug, interleukin 18 protein, human, lipopolysaccharide, cell, community structure, digestive system, genetic analysis, immune system, microbial community, microorganism, neonate, physiology, amplicon, Article, bacterial strain, cesarean section, DNA sequence, female, gene function, genetic variability, human, human cell, human tissue, immunostimulation, intestine flora, marker gene, metagenomics, microbial colonization, newborn, quantitative analysis, real time polymerase chain reaction, sequence homology, taxonomy, vagina flora, vaginal delivery, genetics, in vitro study, metabolism, obstetric delivery, pregnancy, procedures, vertical transmission, Delivery, Obstetric, Gastrointestinal Microbiome, Humans, In Vitro Techniques, Infant, Newborn, Infectious Disease Transmission, Vertical, Interleukin-18, Lipopolysaccharides, Tumor Necrosis Factor-alpha
National Category
Basic Medicine
Identifiers
urn:nbn:se:kth:diva-247015 (URN)10.1038/s41467-018-07631-x (DOI)000451741900005 ()2-s2.0-85057604219 (Scopus ID)
Note

QC 20190626

Available from: 2019-06-26 Created: 2019-06-26 Last updated: 2019-06-26Bibliographically approved
Rinne, H., Bjorkman, U., Sjöqvist, C., Salovius-Lauren, S. & Mattila, J. (2018). Morphological and genetic variation of Fucus in the eastern Gulf of Bothnia, northern Baltic Sea. European journal of phycology, 53(3), 369-380
Open this publication in new window or tab >>Morphological and genetic variation of Fucus in the eastern Gulf of Bothnia, northern Baltic Sea
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2018 (English)In: European journal of phycology, ISSN 0967-0262, E-ISSN 1469-4433, Vol. 53, no 3, p. 369-380Article in journal (Refereed) Published
Abstract [en]

Fucus vesiculosus and F. radicans (Phaeophyceae) are important habitat-formers on rocky shores in the Bothnian Sea. While both species occur sympatrically along the entire western Bothnian Sea coast, F. radicans has been found only in the northern part of the eastern coast. According to previous studies, the two species can be distinguished based on morphology, F. radicans having narrower thalli and a bushier appearance. However, marine mapping in the eastern Bothnian Sea has revealed that high morphological variation in Fucus, partly caused by gradients in salinity and exposure, makes differentiation between the two species difficult. We studied morphological and genetic variation to find out whether the two Fucus species can be differentiated in the south-eastern Bothnian Sea, and if F. radicans occurs in the area. The study was carried out in six subareas including 350 km of coast, witha salinity gradient of 3.5-6.5 PSU, and varying wave exposure. We found a gradual change towards smaller and narrower thalli and a higher number of holdfasts in Fucus populations when moving northwards to lower salinities. Distinct Fucus morphs were often found within the study sites but the morphs were genetically differentiated only at one study site in the Skaftung subarea, suggesting the occurrence of both species. However, in the Vasa subarea the sample size for analysing genetic differentiation was low due to high clonality. In the Luvia subarea south of Skaftung, Fucus morphology corresponded to that of F. radicans in earlier studies but the population was genetically more similar to F. vesiculosus in the southern subareas. We conclude that by using only morphological characteristics it is not possible to differentiate between the two species in central and northern parts of the eastern Bothnian Sea. Based on genetic analyses, the southernmost known occurrence of F. radicans in the eastern Bothnian Sea is in Skaftung.

Place, publisher, year, edition, pages
TAYLOR & FRANCIS LTD, 2018
Keywords
asexual reproduction, clones, environmental gradients, macroalgae, microsatellites, species identification
National Category
Biological Systematics
Identifiers
urn:nbn:se:kth:diva-234214 (URN)10.1080/09670262.2018.1453089 (DOI)000441948900010 ()2-s2.0-85047135109 (Scopus ID)
Note

QC 20180905

Available from: 2018-09-05 Created: 2018-09-05 Last updated: 2018-09-05Bibliographically approved
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