Open this publication in new window or tab >>Stanford Univ, Program Epithelial Biol, Sch Med, Stanford, CA 94305 USA..
Stanford Univ, Program Epithelial Biol, Sch Med, Stanford, CA 94305 USA..
Stanford Univ, Program Epithelial Biol, Sch Med, Stanford, CA 94305 USA..
Stanford Univ, Inst Stem Cell Biol & Regenerat Med, Sch Med, Stanford, CA 94305 USA..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USA..
Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USA..
Univ Calif San Francisco UCSF, Dept Neurol, San Francisco, CA 94122 USA..
Stanford Univ, Program Epithelial Biol, Sch Med, Stanford, CA 94305 USA..
Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USA..
Stanford Univ, Program Epithelial Biol, Sch Med, Stanford, CA 94305 USA..
Stanford Univ, Program Epithelial Biol, Sch Med, Stanford, CA 94305 USA..
Stanford Univ, Dept Microbiol & Immunol, Sch Med, Stanford, CA 94305 USA..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Gene Technology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Stanford Univ, Program Epithelial Biol, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Stanford Canc Inst, Sch Med, Stanford, CA 94305 USA.;Vet Affairs Palo Alto Healthcare Syst, Palo Alto, CA 94304 USA..
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2020 (English)In: Cell, ISSN 0092-8674, E-ISSN 1097-4172, Vol. 182, no 2, p. 497-+Article in journal (Refereed) Published
Abstract [en]
To define the cellular composition and architecture of cutaneous squamous cell carcinoma (cSCC), we combined single-cell RNA sequencing with spatial transcriptomics and multiplexed ion beam imaging from a series of human cSCCs and matched normal skin. cSCC exhibited four tumor subpopulations, three recapitulating normal epidermal states, and a tumor-specific keratinocyte (TSK) population unique to cancer, which localized to a fibrovascular niche. Integration of single-cell and spatial data mapped ligand-receptor networks to specific cell types, revealing TSK cells as a hub for intercellular communication. Multiple features of potential immunosuppression were observed, including T regulatory cell (Treg) co-localization with CD8 T cells in compartmentalized tumor stroma. Finally, single-cell characterization of human tumor xenografts and in vivo CRISPR screens identified essential roles for specific tumor subpopulation-enriched gene networks in tumorigenesis. These data define cSCC tumor and stromal cell subpopulations, the spatial niches where they interact, and the communicating gene networks that they engage in cancer.
Place, publisher, year, edition, pages
Cell Press, 2020
National Category
Cancer and Oncology
Identifiers
urn:nbn:se:kth:diva-279217 (URN)10.1016/j.cell.2020.05.039 (DOI)000552745000018 ()32579974 (PubMedID)2-s2.0-85087696271 (Scopus ID)
Note
QC 20200818
2020-08-182020-08-182022-06-26Bibliographically approved