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Publications (10 of 19) Show all publications
Chang, T., Leygraf, C., Herting, G., Fan, Y., Babu, P., Malkoch, M., . . . Odnevall, I. (2024). Effect of blue light illumination on atmospheric corrosion and bacterial adhesion on copper. Corrosion Science, 230, Article ID 111909.
Open this publication in new window or tab >>Effect of blue light illumination on atmospheric corrosion and bacterial adhesion on copper
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2024 (English)In: Corrosion Science, ISSN 0010-938X, E-ISSN 1879-0496, Vol. 230, article id 111909Article in journal (Refereed) Published
Abstract [en]

The effect of blue light on atmospheric corrosion of Cu and on the antimicrobial properties was explored upon exposure mimicking the condition of hygienic surface disinfection. The results show that blue light illumination enhanced the formation of Cu2O, resulting in a slightly increased corrosion resistance of Cu without pre-deposited NaCl, whereas the enhanced formation of Cu2O, CuCl and/or Cu(OH)3Cl on copper with pre-deposited NaCl caused concomitant corrosion product flaking and a reduced corrosion resistance. The blue light induced enhancement of Cu corrosion led to increased surface roughness and more pronounced integration of bacteria within the corrosion products.

Place, publisher, year, edition, pages
Elsevier BV, 2024
Keywords
Atmospheric corrosion, Bacteria (E. coli), Blue light, Chloride, Copper
National Category
Surface- and Corrosion Engineering
Identifiers
urn:nbn:se:kth:diva-343995 (URN)10.1016/j.corsci.2024.111909 (DOI)001185810700001 ()2-s2.0-85185492077 (Scopus ID)
Note

QC 20240229

Available from: 2024-02-28 Created: 2024-02-28 Last updated: 2025-02-09Bibliographically approved
Biscari, G., Malkoch, M., Fiorica, C., Fan, Y., Palumbo, F. S., Indelicato, S., . . . Pitarresi, G. (2024). Gellan gum-dopamine mediated in situ synthesis of silver nanoparticles and development of nano/micro-composite injectable hydrogel with antimicrobial activity. International Journal of Biological Macromolecules, 258, Article ID 128766.
Open this publication in new window or tab >>Gellan gum-dopamine mediated in situ synthesis of silver nanoparticles and development of nano/micro-composite injectable hydrogel with antimicrobial activity
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2024 (English)In: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 258, article id 128766Article in journal (Refereed) Published
Abstract [en]

Infected skin wounds represent a serious health threat due to the long healing process and the risk of colonization by multi-drug-resistant bacteria. Silver nanoparticles (AgNPs) have shown broad-spectrum antimicrobial activity. This study introduces a novel approach to address the challenge of infected skin wounds by employing gellan gum-dopamine (GG-DA) as a dual-functional agent, serving both as a reducing and capping agent, for the in situ green synthesis of silver nanoparticles. Unlike previous methods, this work utilizes a spray-drying technique to convert the dispersion of GG-DA and AgNPs into microparticles, resulting in nano-into-micro systems (AgNPs@MPs). The microparticles, with an average size of approximately 3 μm, embed AgNPs with a 13 nm average diameter. Furthermore, the study explores the antibacterial efficacy of these AgNPs@MPs directly and in combination with other materials against gram-positive and gram-negative bacteria. The versatility of the antimicrobial material is showcased by incorporating the microparticles into injectable hydrogels. These hydrogels, based on oxidized Xanthan Gum (XGox) and a hyperbranched synthetic polymer (HB10K-G5-alanine), are designed with injectability and self-healing properties through Shiff base formation. The resulting nano-into-micro-into-macro hybrid hydrogel emerges as a promising biomedical solution, highlighting the multifaceted potential of this innovative approach in wound care and infection management.

Place, publisher, year, edition, pages
Elsevier BV, 2024
Keywords
Antimicrobial, Dendritic hydrogel, Gellan gum, Nano-into-micro, Silver nanoparticles
National Category
Materials Chemistry
Identifiers
urn:nbn:se:kth:diva-341936 (URN)10.1016/j.ijbiomac.2023.128766 (DOI)001141667800001 ()38096933 (PubMedID)2-s2.0-85180404366 (Scopus ID)
Note

QC 20240108

Available from: 2024-01-08 Created: 2024-01-08 Last updated: 2024-02-06Bibliographically approved
Liu, H., Fan, Y., Zhong, J., Malkoch, M., Cai, Z. & Wang, Z. (2023). Advance in oral delivery of living material. Biomedical Technology, 3, 26-39
Open this publication in new window or tab >>Advance in oral delivery of living material
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2023 (English)In: Biomedical Technology, E-ISSN 2949-723X, Vol. 3, p. 26-39Article, review/survey (Refereed) Published
Abstract [en]

Nowadays, living therapeutics is a promising candidate among various therapies, and oral administration of living therapeutics characterized by universality and safety avail to make the transition from bench to bedside. However, precise delivery and continuous maintenance of cell viability and activity to acquire stable and ideal therapeutic efficacy remain challenging. Living material is the smart integration of the flexibly programmable functionality of biomaterial and the autonomous environmental responsiveness of living elements, heralding a new era of biotherapeutics in addressing human health concerns. Here, our review aims at presenting an overview of the status of the oral delivery of living material from a biological, technical, and practical perspective, describing the clinical significance and potential, the current technological development and dilemma, as well as the prospect.

Place, publisher, year, edition, pages
Elsevier BV, 2023
Keywords
Biomaterial, Gut microbiome, Living material, Oral administration
National Category
Dentistry Materials Engineering
Identifiers
urn:nbn:se:kth:diva-338441 (URN)10.1016/j.bmt.2022.12.003 (DOI)2-s2.0-85149338749 (Scopus ID)
Note

QC 20231116

Available from: 2023-11-16 Created: 2023-11-16 Last updated: 2023-11-16Bibliographically approved
Qin, L., Zhang, Y., Fan, Y. & Li, L. (2023). Cellulose nanofibril reinforced functional chitosan biocomposite films. Polymer testing, 120, 107964, Article ID 107964.
Open this publication in new window or tab >>Cellulose nanofibril reinforced functional chitosan biocomposite films
2023 (English)In: Polymer testing, ISSN 0142-9418, E-ISSN 1873-2348, Vol. 120, p. 107964-, article id 107964Article in journal (Refereed) Published
Abstract [en]

Recently, chitosan has become attractive due to being biodegradable, biocompatible and renewable. However, the weak mechanical properties of chitosan films limit their large-scale application. In this work, a strategy of blending TEMPO, oxidized CNF (TOCN) and chitosan was developed to fabricate nanocomposite films in order to improve the mechanical properties and maintain biocompatibility. The TOCN/chitosan nanocomposite films exhibited excellent optical transmittance (>85%) and extremely high tensile strength of 235 MPa. The good compatibility of TOCN and chitosan chains, good dispersion of chitosan aggregates and the presence of stiff TOCN crystal domains are the main reasons for getting improved mechanical strength of composite films. The films showed good biocompatible properties based on the cell activity assay results. Furthermore, they were stable in PBS buffer for more than 6 months without significant degradation. The TOCN/chitosan nanocomposite films with these excellent properties could be employed in medical applications.

Place, publisher, year, edition, pages
Elsevier BV, 2023
Keywords
TEMPO oxidized CNF, Chitosan, Nanocomposites, Biocompatibility, Mechanical properties
National Category
Paper, Pulp and Fiber Technology
Identifiers
urn:nbn:se:kth:diva-328431 (URN)10.1016/j.polymertesting.2023.107964 (DOI)000992869700001 ()2-s2.0-85148330765 (Scopus ID)
Note

QC 20230612

Available from: 2023-06-12 Created: 2023-06-12 Last updated: 2023-06-12Bibliographically approved
Zhang, Y., Håkansson, J., Fan, Y., Andrén, O. C. J., San Jacinto García, J., Qin, L., . . . Malkoch, M. (2023). Dendritic Nanogels Directed Dual-Encapsulation Topical Delivery System of Antimicrobial Peptides Targeting Skin Infections. Macromolecular Bioscience, 23(4), Article ID 2200433.
Open this publication in new window or tab >>Dendritic Nanogels Directed Dual-Encapsulation Topical Delivery System of Antimicrobial Peptides Targeting Skin Infections
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2023 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 23, no 4, article id 2200433Article in journal (Refereed) Published
Abstract [en]

Antimicrobial peptides (AMPs) are promising antibacterial agents in the fight against multidrug resistant pathogens. However, their application to skin infections is limited by the absence of a realizable topical delivery strategy. Herein, a hybrid hierarchical delivery system for topical delivery of AMPs is accomplished through the incorporation of AMPs into dendritic nanogels (DNGs) and their subsequent embedding into poloxamer gel. The high level of control over the crosslink density and the number of chosen functionalities makes DNGs ideal capsules with tunable loading capacity for DPK-060, a human kininogen-derived AMP. Once embedded into the poloxamer gel, DPK-060 encapsulated in DNGs displays a slower release rate compared to those entrapped directly in the gels. In vitro EpiDerm Skin Irritation Tests show good biocompatibility, while MIC and time-kill curves reveal the potency of the peptide toward Staphylococcus aureus. Anti-infection tests on ex vivo pig skin and in vivo mouse infection models demonstrate that formulations with 0.5% and 1% AMPs significantly inhibit the growth of S. aureus. Similar outcomes are observed for an in vivo mouse surgical site infection model. Importantly, when normalizing the bacteria inhibition to released/free DPK-060 at the wound site, all formulations display superior efficacy compared to DPK-060 in solution.

Place, publisher, year, edition, pages
Wiley, 2023
Keywords
antimicrobial peptide delivery, dendritic nanogels, DPK-060, poloxamer gels
National Category
Polymer Technologies
Identifiers
urn:nbn:se:kth:diva-330058 (URN)10.1002/mabi.202200433 (DOI)000919125200001 ()36639138 (PubMedID)2-s2.0-85146683018 (Scopus ID)
Note

QC 20230626

Available from: 2023-06-26 Created: 2023-06-26 Last updated: 2023-06-26Bibliographically approved
Fan, Y., Lüchow, M., Badria, A., Hutchinson, D. & Malkoch, M. (2023). Placenta Powder-Infused Thiol-Ene PEG Hydrogels as Potential Tissue Engineering Scaffolds. Biomacromolecules, 24(4), 1617-1626
Open this publication in new window or tab >>Placenta Powder-Infused Thiol-Ene PEG Hydrogels as Potential Tissue Engineering Scaffolds
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2023 (English)In: Biomacromolecules, ISSN 1525-7797, E-ISSN 1526-4602, Vol. 24, no 4, p. 1617-1626Article in journal (Refereed) Published
Abstract [en]

Human placenta is a source of extracellular matrix for tissue engineering. In this study, placenta powder (PP), made from decellularized human placenta, was physically incorporated into synthetic poly(ethylene glycol) (PEG)-based hydrogels via UV-initiated thiol-ene coupling (TEC). The PP-incorporated PEG hydrogels (MoDPEG+) showed tunable storage moduli ranging from 1080 ± 290 to 51,400 ± 200 Pa. The addition of PP (1, 4, or 8 wt %) within the PEG hydrogels increased the storage moduli, with the 8 wt % PP hydrogels showing the highest storage moduli. PP reduced the swelling ratios compared with the pristine hydrogels (MoDPEG). All hydrogels showed good biocompatibility in vitro toward human skin cells and murine macrophages, with cell viability above 91%. Importantly, cells could adhere and proliferate on MoDPEG+ hydrogels due to the bioactive PP, while MoDPEG hydrogels were bio-inert as cells moved away from the hydrogel or were distributed in a large cluster on the hydrogel surface. To showcase their potential use in application-driven research, the MoDPEG+ hydrogels were straightforwardly (i) 3D printed using the SLA technique and (ii) produced via high-energy visible light (HEV-TEC) to populate damaged soft-tissue or bone cavities. Taking advantage of the bioactivity of PP and the tunable physicochemical properties of the synthetic PEG hydrogels, the presented MoDPEG+ hydrogels show great promise for tissue regeneration.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2023
National Category
Bio Materials
Identifiers
urn:nbn:se:kth:diva-330964 (URN)10.1021/acs.biomac.2c01355 (DOI)000955460100001 ()36944137 (PubMedID)2-s2.0-85151336832 (Scopus ID)
Note

QC 20230705

Available from: 2023-07-05 Created: 2023-07-05 Last updated: 2023-07-05Bibliographically approved
Lin, J., Fan, Y., Hutchinson, D. & Malkoch, M. (2023). Soft Hydroxyapatite Composites Based on Triazine-Trione Systems as Potential Biomedical Engineering Frameworks. ACS Applied Materials and Interfaces, 15(5), 7329-7339
Open this publication in new window or tab >>Soft Hydroxyapatite Composites Based on Triazine-Trione Systems as Potential Biomedical Engineering Frameworks
2023 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 15, no 5, p. 7329-7339Article in journal (Refereed) Published
Abstract [en]

Composites of triazine-trione (TATO) thiol-ene networks and hydroxyapatite (HA) have shown great potential as topological fixation materials for complex bone fractures due to their high flexural modulus, biocompatibility, and insusceptibility to forming soft-tissue adhesions. However, the rigid mechanical properties of these composites make them unsuitable for applications requiring softness. The scope of these materials could therefore be widened by the design of new TATO monomers that would lead to composites with a range of mechanical properties. In this work, four novel TATO-based monomers, decorated with either ester or amide linkages as well as alkene or alkyne end groups, have been proposed and synthesized via fluoride-promoted esterification (FPE) chemistry. The ester-modified monomers were then successfully formulated along with the thiol TATO monomer tris [2-(3-mercaptopropionyloxy)ethyl] isocyanurate (TEMPIC) and HA to give soft composites, following the established photo-initiated thiol-ene coupling (TEC) or thiol-yne coupling (TYC) chemistry methodologies. The most promising composite shows excellent softness, with a flexural modulus of 57 (2) MPa and ϵf at maximum σf of 11.8 (0.3)%, which are 117 and 10 times softer than the previously developed system containing the commercially available tri-allyl TATO monomer (TATATO). Meanwhile, the surgically convenient viscosity of the composite resins and their excellent cytotoxicity profile allow them to be used in the construction of soft objects in a variety of shapes through drop-casting suitable for biomedical applications.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2023
Keywords
biocompatibility, biomedical engineering, soft hydroxyapatite composites, thiol−ene and thiol−yne materials, triazine−trione materials
National Category
Polymer Chemistry
Identifiers
urn:nbn:se:kth:diva-330044 (URN)10.1021/acsami.2c16235 (DOI)000931726500001 ()36695708 (PubMedID)2-s2.0-85147151493 (Scopus ID)
Note

QC 20230627

Available from: 2023-06-27 Created: 2023-06-27 Last updated: 2024-05-03Bibliographically approved
Wang, H., Fan, Y., Yang, Q., Sun, X., Liu, H., Chen, W., . . . Wang, S. (2022). Boosting the Electrochemical Performance of PI-5-CA/C-SWCNT Nanohybrid for Sensitive Detection of E. coli O157:H7 From the Real Sample. Frontiers in Chemistry, 10, Article ID 843859.
Open this publication in new window or tab >>Boosting the Electrochemical Performance of PI-5-CA/C-SWCNT Nanohybrid for Sensitive Detection of E. coli O157:H7 From the Real Sample
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2022 (English)In: Frontiers in Chemistry, E-ISSN 2296-2646, Vol. 10, article id 843859Article in journal (Refereed) Published
Abstract [en]

Redox activity is an important indicator for evaluating electrochemical biosensors. In this work, we have successfully polymerized indole-5-carboxylic acid into poly-5-carboxyindole nanomaterials (PI-5-CA), using its superior redox activity, and introduced carboxylated single-walled carbon nanotubes (C-SWCNTs) to synthesize a composite material. Finally, a synthesized composite material was used for the modification of the glass carbon electrode to fabricate the PI-5-CA/C-SWCNTs/GCE-based immunosensor and was successfully applied for the sensitive detection of E. coli O157:H7. The fabricated immunosensor exhibited an outstanding electrocatalytic activity toward the detection of E. coli O157:H7 with a remarkably lowest limit of detection (2.5 CFU/ml, LOD = 3 SD/k, n = 3) and has a wide linear range from 2.98x10(1) to 2.98x10(7) CFU/ml. Inspired from the excellent results, the fabricated electrode was applied for the detection of bacteria from real samples (water samples) with a good recovery rate (98.13-107.69%) as well as an excellent stability and specificity. Owing to its simple preparation, excellent performance, and detection time within 30 min, our proposed immunosensor will open a new horizon in different fields for the sensitive detection of bacteria from real samples.

Place, publisher, year, edition, pages
Frontiers Media SA, 2022
Keywords
poly-5-carboxyindole, carboxylated single-walled carbon nanotubes, E. coli O157:H7, electrochemical immunosensor, indole-5-carboxylic acid, plate counting method
National Category
Food Science Microbiology Microbiology in the medical area
Identifiers
urn:nbn:se:kth:diva-309809 (URN)10.3389/fchem.2022.843859 (DOI)000761050700001 ()35223774 (PubMedID)2-s2.0-85125264323 (Scopus ID)
Note

QC 20220315

Available from: 2022-03-15 Created: 2022-03-15 Last updated: 2022-06-25Bibliographically approved
Fan, Y., Mohanty, S., Zhang, Y., Lüchow, M., Qin, L., Fortuin, L., . . . Malkoch, M. (2021). Dendritic Hydrogels Induce Immune Modulation in Human Keratinocytes and Effectively Eradicate Bacterial Pathogens. Journal of the American Chemical Society, 143(41), 17180-17190
Open this publication in new window or tab >>Dendritic Hydrogels Induce Immune Modulation in Human Keratinocytes and Effectively Eradicate Bacterial Pathogens
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2021 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 143, no 41, p. 17180-17190Article in journal (Refereed) Published
Abstract [en]

Infections caused by antibiotic-resistant bacteria are globally a major threat, leading to high mortality rates and increased economic burden. Novel treatment strategies are therefore urgently needed by healthcare providers to protect people. Biomaterials that have inherent antibacterial properties and do not require the use of antibiotics present an attractive and feasible avenue to achieve this goal. Herein, we demonstrate the effect of a new class of cationic hydrogels based on amino-functional hyperbranched dendritic-linear-dendritic copolymers (HBDLDs) exhibiting excellent antimicrobial activity toward a wide range of clinical Gram-positive and Gram-negative bacteria, including drug-resistant strains isolated from wounds. Intriguingly, the hydrogels can induce the expression of the antimicrobial peptides RNase 7 and psoriasin, promoting host-mediated bacterial killing in human keratinocytes (HaCaT). Moreover, treatment with the hydrogels decreased the proinflammatory cytokine IL-1 beta, reactive nitrogen species (NO), and mitochondrial reactive oxygen species (ROS) in S. aureus-infected HaCaT cells, conjunctively resulting in reduced inflammation.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2021
National Category
Infectious Medicine
Identifiers
urn:nbn:se:kth:diva-305669 (URN)10.1021/jacs.1c07492 (DOI)000710935500033 ()34636555 (PubMedID)2-s2.0-85118244398 (Scopus ID)
Note

QC 20211206

Available from: 2021-12-06 Created: 2021-12-06 Last updated: 2022-06-25Bibliographically approved
Fan, Y., Lüchow, M., Zhang, Y., Lin, J., Mohanty, S., Brauner, A., . . . Fortuin, L. (2021). Nanogel encapsulated hydrogels as advanced wound dressings for the controlled delivery of antibiotics. Advanced Functional Materials, 31
Open this publication in new window or tab >>Nanogel encapsulated hydrogels as advanced wound dressings for the controlled delivery of antibiotics
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2021 (English)In: Advanced Functional Materials, ISSN 1616-301X, Vol. 31Article in journal (Refereed) Published
Abstract [en]

Biocompatible and degradable dual-delivery gel systems based on hyperbrancheddendritic−linear−dendritic copolymers (HBDLDs) is herein conceptualizedand accomplished via thiol-ene click chemistry. The elasticity of thehydrogels is tunable by varying the lengths of PEG (2, 6, 10 kDa) or the dryweight percentages (20, 30, 40 wt%), and are found to be between 2–14.7 kPa,comparable to human skin. The co-delivery of antibiotics is achieved, wherethe hydrophilic drug novobiocin sodium salt (NB) is entrapped within thehydrophilic hydrogel, while the hydrophobic antibiotic ciprofloxacin (CIP) isencapsulated within the dendritic nanogels (DNGs) with hydrophobic cores(DNGs-CIP). The DNGs-CIP with drug loading capacity of 2.83 wt% are thenphysically entrapped within the hybrid hydrogels through UV curing. Thehybrid hydrogels enabled the quick release of NB and prolonged released ofCIP. In vitro cell infection assays showed that the antibiotic-loaded hybridhydrogels are able to treat bacterial infections with significant bacterialreduction. Hybrid hydrogel band aids are fabricated and exhibited betterantibacterial activity compared with commercial antimicrobial band aids.Remarkably, most hydrogels and hybrid hydrogels showed enhanced humandermal cell proliferation and could be degraded into non-toxic constituents,showing great promise as wound dressing materials.

National Category
Natural Sciences
Research subject
Fibre and Polymer Science; Chemical Engineering
Identifiers
urn:nbn:se:kth:diva-293758 (URN)10.1002/adfm.202006453 (DOI)2-s2.0-85094644501 (Scopus ID)
Note

QC 20210623

Available from: 2021-05-23 Created: 2021-05-23 Last updated: 2022-06-25Bibliographically approved
Organisations
Identifiers
ORCID iD: ORCID iD iconorcid.org/0000-0001-7639-1173

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