This manuscript demonstrates the first successful application of the delayed-x-LMS (dxLMS) control algorithm for TS-wave cancelation. Active wave cancelation of two-dimensional broadband Tollmien–Schlichting (TS) disturbances is performed with a single DBD plasma actuator. The experiments are conducted in flight on the pressure side of a laminar flow wing glove, mounted on a manned glider. The stability properties of the controller are investigated in detail with experimental flight data, DNS and stability analysis of the boundary layer. Finally, a model-free approach for dxLMS operation is introduced to operate the controller as a ‘black-box’ system, which automatically adjusts the controller settings based on a group speed measurement of the disturbance wave packets. The modified dxLMS controller is operated without a model and is able to adapt to varying conditions that may occur during flight in atmosphere.