Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Wearable All-Solid-State Potentiometric Microneedle Patch for Intradermal Potassium Detection
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0002-1344-8432
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0002-3858-8466
Karolinska Inst, Dept Clin Sci Intervent & Technol, K 57, SE-14186 Stockholm, Sweden.;Karolinska Univ Sjukhuset, Div Obstet & Gynecol, S-14186 Stockholm, Sweden..
KTH, School of Electrical Engineering and Computer Science (EECS), Micro and Nanosystems.ORCID iD: 0000-0001-7769-3931
Show others and affiliations
2019 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 91, no 2, p. 1578-1586Article in journal (Refereed) Published
Abstract [en]

A new analytical all-solid-state platform for intradermal potentiometric detection of potassium in interstitial fluid is presented here. Solid microneedles are modified with different coatings and polymeric membranes to prepare both the potassium-selective electrode and reference electrode needed for the potentiometric readout. These microneedle-based electrodes are fixed in an epidermal patch suitable for insertion into the skin. The analytical performances observed for the potentiometric cell (Nernstian slope, limit of detection of 10(-4.9) potassium activity, linear range of 10(-4.2) to 10(-1.1), drift of 0.35 +/- 0.28 mV h(-1)), together with a fast response time, adequate selectivity, and excellent reproducibility and repeatability, are appropriate for potassium analysis in interstitial fluid within both clinical and harmful levels. The potentiometric response is maintained after several insertions into animal skin, confirming the resiliency of the microneedle-based sensor. Ex vivo tests based on the intradermal detection of potassium in chicken and porcine skin demonstrate that the microneedle patch is suitable for monitoring potassium changes inside the skin. In addition, the dimensions of the microneedles modified with the corresponding layers necessary to enhance robustness and provide sensing capabilities (1000 mu m length, 45 degrees tip angle, 15 mu m thickness in the tip, and 435 mu m in the base) agree with the required ranges for a painless insertion into the skin. In vitro cytotoxicity experiments showed that the patch can be used for at least 24 h without any side effect for the skin cells. Overall, the developed concept constitutes important progress in the intradermal analysis of ions related to an electrolyte imbalance in humans, which is relevant for the control of certain types of diseases.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2019. Vol. 91, no 2, p. 1578-1586
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-243957DOI: 10.1021/acs.analchem.8b04877ISI: 000456350000049PubMedID: 30543102Scopus ID: 2-s2.0-85059747630OAI: oai:DiVA.org:kth-243957DiVA, id: diva2:1293031
Note

QC 20190301

Available from: 2019-03-01 Created: 2019-03-01 Last updated: 2019-03-06Bibliographically approved
In thesis
1. Flexible and Stretchable Biointerfacing for Healthcare Diagnostics
Open this publication in new window or tab >>Flexible and Stretchable Biointerfacing for Healthcare Diagnostics
2019 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

Flexible and stretchable wearable biomedical devices provide a platform for continues long-term monitoring of biological signals during neutral body movements thus enabling early intervention and diagnostics of various diseases. This thesis evaluates novel flexible and stretchable bio interfacing medical devices based on microneedle patches and split ring resonator for healthcare diagnostics. Flexible and stretchable microneedle patches were realized by integrating a soft polymer substrate with sharp stainless steel microneedles. This was realized using a magnetic assembly technique. Investigations have shown that the flexible microneedle patch can provide conformal and reliable contact with wrinkles and deformations of the skin. In addition, transdermal monitoring of potassium ions using the proposed flexible microneedle patch have been demonstrated by coating the microneedles with a potassium sensing membrane. Ex-vivo test on the microneedle potassium sensor performed on chicken and porcine skin was able to detect change in potassium concentration in the skin. Furthermore, a novel flexible bio-interface spilt ring resonator (SRR) for the monitoring of intera cranial pressure (ICP) is demonstrated. The sensor was fabricated by depositing a 500 nm gold film on a thermoset thiolene epoxy polymer substrate. The flexible sensor was able to clearly detect the pressure variation that might be an indication of increased ICP in the skull. The proposed methodology of heterogeneous integration of hard materials on a soft and flexible substrate demonstrates a first proof of concept of flexible wearable bio-interfacing devices with vastly different material properties with the potential for continuous and real-time health monitoring.

Place, publisher, year, edition, pages
KTH Royal Institute of Technology, 2019. p. 36
Series
TRITA-EECS-AVL ; 2019:22
Keywords
Wearable biointerfacing sensor, flexible and stretchable microneedles patch, wearable microneedles device, health and fitness monitoring, minimally invasive ICP monitoring, bioelectronics
National Category
Engineering and Technology
Research subject
Electrical Engineering
Identifiers
urn:nbn:se:kth:diva-245075 (URN)978-91-7873-128-2 (ISBN)
Presentation
2019-03-27, Q34, Kungliga Tekniska högskolan, Malvinas väg 6, Stockholm, 10:00 (English)
Opponent
Supervisors
Note

QC 20190306

Available from: 2019-03-06 Created: 2019-03-05 Last updated: 2019-10-09Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Parrilla, MarcCuartero, MariaRajabi, MinaRoxhed, NiclasNiklaus, FrankCrespo, Gaston A.

Search in DiVA

By author/editor
Parrilla, MarcCuartero, MariaRajabi, MinaRoxhed, NiclasNiklaus, FrankCrespo, Gaston A.
By organisation
Applied Physical ChemistryMicro and Nanosystems
In the same journal
Analytical Chemistry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 169 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf