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Analysis of butterfly reproductive proteins using capillary electrophoresis and mass spectrometry
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Applied Physical Chemistry.
Uppsala Univ, Dept Ecol & Genet Anim Ecol, Uppsala, Sweden..
Stockholm Univ, Dept Zool, Ecol, Stockholm, Sweden..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Applied Physical Chemistry.ORCID iD: 0000-0002-3444-9987
2019 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 566, p. 23-26Article in journal (Refereed) Published
Abstract [en]

A method for analysis of proteins from spermatophores transferred from male to female Pieris napi butterflies during mating has been developed. The proteins were solubilized from the dissected spermatophores using different solubilization agents (water, methanol, acetonitrile and hexafluoroisopropanol). Capillary electrophoresis (CE) analysis was performed using an acidic background electrolyte containing a fluorosurfactant to avoid protein-wall adsorption, and to increase separation performance. The samples were also analyzed with matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), in a lower m/z range (1000-6000) and a higher m/z range (6000-12000). Solubilization with different solvents and the use of alternative matrices gave partly complementary profiles.

Place, publisher, year, edition, pages
ACADEMIC PRESS INC ELSEVIER SCIENCE , 2019. Vol. 566, p. 23-26
Keywords [en]
Capillary electrophoresis, Matrix assisted laser desorption/ionization, mass spectrometry, Pieris napi, Seminal fluid proteins, Spermatophore
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-243942DOI: 10.1016/j.ab.2018.11.002ISI: 000456354900006PubMedID: 30423321Scopus ID: 2-s2.0-85056596095OAI: oai:DiVA.org:kth-243942DiVA, id: diva2:1293975
Note

QC 20190306

Available from: 2019-03-06 Created: 2019-03-06 Last updated: 2019-03-06Bibliographically approved

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Rokhas, Maria KhihonEmmer, Åsa

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