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Comparative characterization of putative chitin deacetylases from Phaeodactylum tricornutum and Thalassiosira pseudonana highlights the potential for distinct chitin-based metabolic processes in diatoms
Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.;PSL Res Univ, Ecole Normale Super, IBENS, CNRS,INSERM, F-75005 Paris, France.;Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China.;Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China..ORCID iD: 0000-0002-9196-0175
PSL Res Univ, Ecole Normale Super, IBENS, CNRS,INSERM, F-75005 Paris, France..
Westphalian Wilhelms Univ Munster, Inst Plant Biol & Biotechnol, D-48143 Munster, Germany..ORCID iD: 0000-0002-1575-7423
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH). Royal Inst Technol KTH, AlbaNova Univ Ctr, Sch Biotechnol, Div Glycosci, SE-10691 Stockholm, Sweden..
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2019 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 221, no 4, p. 1890-1905Article in journal (Refereed) Published
Abstract [en]

Chitin is generally considered to be present in centric diatoms but not in pennate species. Many aspects of chitin biosynthetic pathways have not been explored in diatoms. We retrieved chitin metabolic genes from pennate (Phaeodactylum tricornutum) and centric (Thalassiosira pseudonana) diatom genomes. Chitin deacetylase (CDA) genes from each genome (PtCDA and TpCDA) were overexpressed in P. tricornutum. We performed comparative analysis of their sequence structure, phylogeny, transcriptional profiles, localization and enzymatic activities. The chitin relevant proteins show complex subcellular compartmentation. PtCDA was likely acquired by horizontal gene transfer from prokaryotes, whereas TpCDA has closer relationships with sequences in Opisthokonta. Using transgenic P. tricornutum lines expressing CDA-green fluorescent protein (GFP) fusion proteins, PtCDA predominantly localizes to Golgi apparatus whereas TpCDA localizes to endoplasmic reticulum/chloroplast endoplasmic reticulum membrane. CDA-GFP overexpression upregulated the transcription of chitin synthases and potentially enhanced the ability of chitin synthesis. Although both CDAs are active on GlcNAc(5), TpCDA is more active on the highly acetylated chitin polymer DA60. We have addressed the ambiguous characters of CDAs from P. tricornutum and T. pseudonana. Differences in localization, evolution, expression and activities provide explanations underlying the greater potential of centric diatoms for chitin biosynthesis. This study paves the way for in vitro applications of novel CDAs.

Place, publisher, year, edition, pages
WILEY , 2019. Vol. 221, no 4, p. 1890-1905
Keywords [en]
chitin, chitin deacetylase, chitosan, enzymatic activity, gene transformation, Phaeodactylum tricornutum, subcellular localization, Thalassiosira pseudonana
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-245130DOI: 10.1111/nph.15510ISI: 000458259600023PubMedID: 30288745Scopus ID: 2-s2.0-85056648317OAI: oai:DiVA.org:kth-245130DiVA, id: diva2:1296059
Note

QC 20190313

Available from: 2019-03-13 Created: 2019-03-13 Last updated: 2019-03-13Bibliographically approved

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Bulone, Vincent

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Shao, ZhanruHembach, LeaXing, XiaohuiBulone, VincentBowler, Chris
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