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Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry
KTH, School of Engineering Sciences (SCI), Applied Physics. Karolinska Inst, Dept Clin Neurosci, Ctr Psychiat Res, SE-17176 Solna, Sweden ; Univ Bu Ali Sina, Dept Chem, Hamadan 65174, Iran.
Univ Bu Ali Sina, Dept Chem, Hamadan 65174, Iran..
KTH, School of Engineering Sciences (SCI), Applied Physics.ORCID iD: 0000-0001-6288-7709
Karolinska Inst, Dept Clin Neurosci, Ctr Psychiat Res, SE-17176 Solna, Sweden..
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2019 (English)In: Molecules, ISSN 1431-5157, E-ISSN 1420-3049, Vol. 24, no 7, article id 1191Article in journal (Refereed) Published
Abstract [en]

In this study, a novel sort of sample preparation sorbent was developed, by preparing thin layer graphene oxide tablets (GO-Tabs) utilizing a mixture of graphene oxide and polyethylene glycol on a polyethylene substrate. The GO-Tabs were used for extraction and concentration of omeprazole (OME) in human saliva samples. The determination of OME was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) under gradient LC conditions and in the positive ion mode (ESI+) with mass transitions of m/z 346.3 -> 198.0 for OME and m/z 369.98 -> 252.0 for the internal standard. Standard calibration for the saliva samples was in the range of 2.0-2000 nmol L-1. Limits of detection and quantification were 0.05 and 2.0 nmol L-1, respectively. Method validation showed good method accuracy and precision; the inter-day precision values ranged from 5.7 to 8.3 (%RSD), and the accuracy of determinations varied from -11.8% to 13.3% (% deviation from nominal values). The extraction recovery was 60%, and GO-Tabs could be re-used for more than ten extractions without deterioration in recovery. In this study, the determination of OME in real human saliva samples using GO-Tab extraction was validated.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 24, no 7, article id 1191
Keywords [en]
graphene oxide, omeprazole, liquid chromatography, tandem mass spectrometry, saliva, GO-Tabs
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-251343DOI: 10.3390/molecules24071191ISI: 000464946300002PubMedID: 30934659Scopus ID: 2-s2.0-85063759590OAI: oai:DiVA.org:kth-251343DiVA, id: diva2:1317081
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QC 20190521

Available from: 2019-05-21 Created: 2019-05-21 Last updated: 2023-12-07Bibliographically approved

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Zohdi, ZeynabUheida, AbdusalamAbdel-Rehim, Mohamed

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