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Cytotoxic effect of crude and purified pectins from Campomanesia xanthocarpa Berg on human glioblastoma cells
Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil..
Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Glycoscience. UF, GCREC, Dept Entomol & Nematol, Wimauma, FL USA..ORCID iD: 0000-0002-8533-1022
Univ Fed Parana, Sect Biol Sci, Postgrad Program Biochem Sci, BR-81531990 Curitiba, PR, Brazil..
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2019 (English)In: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 224, article id UNSP 115140Article in journal (Refereed) Published
Abstract [en]

A new source of pectin with a cytotoxic effect on glioblastoma cells is presented. A homogeneous GWP-FP-S fraction (M-w, of 29,170 g mol(-1)) was obtained by fractionating the crude pectin extract (GW) from Campomanesia xanthocarpa pulp. According to the monosaccharide composition, the GWP-FP-S was composed of galacturonic acid (58.8%), arabinose (28.5%), galactose (11.3%) and rhamnose (1.1%), comprising 57.7% of homogalacturonans (HG) and 42.0% of type I rhamnogalacturonans (RG-I). These structures were characterized by chromatographic and spectroscopic methods; GW and GWP-FP-S fractions were evaluated by MIT and crystal violet assays for their cytotoxic effects. Both fractions induced cytotoxicity (15.55-37.65%) with concomitant increase in the cellular ROS levels in human glioblastoma cells at 25-400 mu g mL(-)(1), after 48 h of treatment, whereas no cytotoxicity was observed for normal NIH 3T3 cells. This is the first report of in vitro bioactivity and the first investigation of the antitumor potential of gabiroba pectins.

Place, publisher, year, edition, pages
ELSEVIER SCI LTD , 2019. Vol. 224, article id UNSP 115140
Keywords [en]
Gabiroba, Pectin, NMR analysis, Glioblastoma cells, Cytotoxicity, ROS
National Category
Polymer Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-260154DOI: 10.1016/j.carbpol.2019.115140ISI: 000484001200012PubMedID: 31472853Scopus ID: 2-s2.0-85070215128OAI: oai:DiVA.org:kth-260154DiVA, id: diva2:1356224
Note

QC 20191001

Available from: 2019-10-01 Created: 2019-10-01 Last updated: 2019-10-01Bibliographically approved

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Ruthes, Andrea C.

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