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Functionalized graphene oxide tablets for sample preparation of drugs in biological fluids: Extraction of ritonavir, a HIV protease inhibitor, from human saliva and plasma using LC–MS/MS
Karolinska institutet.
KTH, School of Engineering Sciences (SCI), Applied Physics, Materials and Nanophysics.ORCID iD: 0000-0001-9424-6965
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2021 (English)In: BMC Biomedical chromotography, ISSN 0269-3879, E-ISSN 1099-0801, Vol. 35, no 12, article id e5111Article in journal (Refereed) Published
Abstract [en]

In this work, graphene oxide–based tablets (GO-Tabs) were prepared by applying a thin layer of functionalized GO on a polyethylene substrate. The GO was functionalized with amine groups (–NH2) by poly(ethylene glycol)bis(3-aminopropyl) terminated (GO-NH2-PEG-NH2). The functionalized GO-Tabs were used for the extraction of ritonavir (RTV) in human saliva samples. RTV in plasma and saliva samples was analyzed using LC–MS/MS. Gradient LC system with MS/MS in the positive-ion mode [electrospray ionization (ESI+)] was used. The transitions m/z 721 → 269.0 and m/z 614 → 421 were used for RTV and the internal standard indinavir, respectively. This study determined the human immunodeficiency virus protease inhibitor RTV in human saliva samples using functionalized GO-Tab and LC–MS/MS, and the method was validated. The standard calibration curve for plasma and saliva samples was constructed from 5.0 to 2000 nmol L−1. The limit of detection was 0.1 nmol L−1, and the limit of quantification was 5.0 nmol L−1 in both plasma and saliva matrices. The intra- and inter-assay precision values were found to be between 1.5 and 5.8%, and the accuracy values ranged from 88.0 to 108% utilizing saliva and plasma samples. The extraction recovery was more than 80%, and the presented functionalized GO-Tabs could be reused for more than 10 extractions without deterioration in recovery.

Place, publisher, year, edition, pages
Wiley , 2021. Vol. 35, no 12, article id e5111
Keywords [en]
graphene oxide tablets, LC–MS/MS, plasma, ritonavir, saliva, amine, graphene oxide, macrogol derivative, nanocomposite, poly(ethylene glycol)bis (3 aminopropyl), polyethylene, unclassified drug, graphite, Human immunodeficiency virus proteinase inhibitor, nanomaterial, blood sampling, Conference Paper, drug determination, drug synthesis, human, hydrogen bond, limit of detection, limit of quantitation, liquid chromatography-mass spectrometry, positive ion electrospray, saliva analysis, tablet, chemistry, liquid chromatography, procedures, reproducibility, statistical model, tablet manufacture, tandem mass spectrometry, Chromatography, Liquid, HIV Protease Inhibitors, Humans, Linear Models, Nanostructures, Reproducibility of Results, Tablets
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-309668DOI: 10.1002/bmc.5111ISI: 000656746500001PubMedID: 33675066Scopus ID: 2-s2.0-85107333551OAI: oai:DiVA.org:kth-309668DiVA, id: diva2:1644567
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QC 20220314

Available from: 2022-03-14 Created: 2022-03-14 Last updated: 2024-03-18Bibliographically approved

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Laxman, KarthikUheida, AbdusalamDutta, JoydeepAbdel-Rehim, Mohamed

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