The cellulose nanofiber(CNF)-shelled perfluoropentane (PFP) droplets were the subject of the evaluation in this work. CNF-shelled PFP droplets  Mechanical and acoustic properties were studied previously and reported elsewhere [1-4], however more insights into the biological effects of these droplets are needed to translate previous in vitro tests into in vivo applications as drug carriers in ultrasound-mediated drug delivery. 

The aim of this work is to investigate the effect of CNF-shelled PFP droplets on cell viability of 4T1 cells, a murine breast cancer cell line. Cells were exposed to different concentrations of droplets with or without the addition of paclitaxel, i.e. a hydrophobic cancer drug.

[1].  Ghorbani M, Olofsson K, Benjamins J-W, Loskutova K, Paulraj T, Wiklund M, Grishenkov D, Svagan A J, Unravelling the Acoustic and Thermal Responses of Perfluorocarbon Liquid Droplets Stabilized with Cellulose Nanofibers, Langmuir 35(40): 13090-13099, 2019.

[2].  Song X, Loskutova K, Chen H, Shen G, Grishenkov D, Deriving acoustic properties for perfluoropentane droplets with viscoelastic cellulose nanofiber shell via numerical simulations, The Journal of the Acoustical Society of America 150(3): 1750-1761, 2021.

[3].  Loskutova K, Nimander D, Gouwy I, Chen H, Ghorbani M, Svagan A J, Grishenkov D, A Study on the Acoustic Response of Pickering Perfluoropentane Droplets in Different Media, ACS Omega 6(8): 5670-5678, 2019.

[4].  Loskutova K, Olofsson K, Hammarström B, Wiklund M, Svagan A J, Grishenkov D, Measuring the Compressibility of Cellulose Nanofiber-Stabilized Microdroplets Using Acoustophoresis, Micromachines 12(12): 1465, 2021.