The increasing global demand for agricultural production poses challenges to maintain the needs for critical fertilizers such as nitrogen. This study explores the potential of human urine as a source of renewable nitrogen for fertilizer production. Through a life cycle assessment, three different urine management strategies were compared: (S1) an artificial wetland, (S2) an on-site lab-scale aerobic reactor for nitrogen recovery, and (S3) a centralized wastewater treatment plant. While scenario S2 had the highest impacts in 6 out of 8 categories, an advantage in marine eutrophication was identified. S2 showed high energy demand (750 kg MJ-eq) and ecotoxicity (602 kg 1.4-DCB-eq.) mainly due to energy requirements. Nitrogen production exceeded 2.3 times the yearly nitrogen demands of the building tomato production. Upscaling S2 reduces impacts up to 2 times, lowering the payback time from 29 to 13 years. Therefore, implementing large-scale nitrogen recovery systems in cities is encouraged.