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AKAP79 enables calcineurin to directly suppress protein kinase A activity
UCL, Dept Neurosci Physiol & Pharmacol, London, England..ORCID iD: 0000-0002-5958-6304
KTH, School of Electrical Engineering and Computer Science (EECS), Computer Science, Computational Science and Technology (CST). KTH, Centres, Science for Life Laboratory, SciLifeLab. Karolinska Inst, Dept Neurosci, Stockholm, Sweden..
UCL, Dept Neurosci Physiol & Pharmacol, London, England..ORCID iD: 0000-0003-4594-0808
KTH, School of Electrical Engineering and Computer Science (EECS). KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-9635-5145
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2021 (English)In: eLIFE, E-ISSN 2050-084X, Vol. 10, article id e68164Article in journal (Refereed) Published
Abstract [en]

Interplay between the second messengers cAMP and Ca2+ is a hallmark of dynamic cellular processes. A common motif is the opposition of the Ca2+-sensitive phosphatase calcineurin and the major cAMP receptor, protein kinase A (PKA). Calcineurin dephosphorylates sites primed by PKA to bring about changes including synaptic long-term depression (LTD). AKAP79 supports signaling of this type by anchoring PKA and calcineurin in tandem. In this study, we discovered that AKAP79 increases the rate of calcineurin dephosphorylation of type II PKA regulatory subunits by an order of magnitude. Fluorescent PKA activity reporter assays, supported by kinetic modeling, show how AKAP79-enhanced calcineurin activity enables suppression of PKA without altering cAMP levels by increasing PKA catalytic subunit capture rate. Experiments with hippocampal neurons indicate that this mechanism contributes toward LTD. This non-canonical mode of PKA regulation may underlie many other cellular processes.

Place, publisher, year, edition, pages
eLIFE SCIENCES PUBL LTD , 2021. Vol. 10, article id e68164
Keywords [en]
protein kinase A, anchoring protein, synaptic plasticity, calcineurin, cyclic AMP, calcium, Rat
National Category
Cell and Molecular Biology Biochemistry Molecular Biology Microbiology
Identifiers
URN: urn:nbn:se:kth:diva-305114DOI: 10.7554/eLife.68164ISI: 000714025800001PubMedID: 34612814Scopus ID: 2-s2.0-85118489018OAI: oai:DiVA.org:kth-305114DiVA, id: diva2:1613402
Note

See also peer review documents at DOI  10.7554/eLife.68164.sa1 and  10.7554/eLife.68164.sa2

QC 20211122

Available from: 2021-11-22 Created: 2021-11-22 Last updated: 2025-02-20Bibliographically approved

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Tewatia, ParulAntunes, JoaoEriksson, OliviaHellgren Kotaleski, Jeanette

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Church, Timothy W.Tewatia, ParulHannan, SaadAntunes, JoaoEriksson, OliviaSmart, Trevor G.Hellgren Kotaleski, JeanetteGold, Matthew G.
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Computational Science and Technology (CST)Science for Life Laboratory, SciLifeLabSchool of Electrical Engineering and Computer Science (EECS)
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