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Multifocus structured illumination microscopy for fast volumetric super-resolution imaging
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.ORCID-id: 0000-0002-5584-9170
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.
Vise andre og tillknytning
2017 (engelsk)Inngår i: Biomedical Optics Express, ISSN 2156-7085, E-ISSN 2156-7085, Vol. 8, nr 9, s. 4135-4140, artikkel-id #294866Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in superresolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens.

sted, utgiver, år, opplag, sider
OSA - The Optical Society , 2017. Vol. 8, nr 9, s. 4135-4140, artikkel-id #294866
Emneord [en]
Fluorescence microscopy, Imaging systems, Microscopy, Superresolution, Three-dimensional microscopy, Diffraction, Fluorescence, Image reconstruction, Light, Mergers and acquisitions, Microscopic examination, Optical resolving power, Biological specimens, Imaging performance, Structured illumination microscopies (SIM), Structured illumination microscopy, Super resolution, Super resolution imaging, Volumetric acquisitions, Magnetic force microscopy
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Identifikatorer
URN: urn:nbn:se:kth:diva-216180DOI: 10.1364/BOE.8.004135ISI: 000409020600017Scopus ID: 2-s2.0-85028755480OAI: oai:DiVA.org:kth-216180DiVA, id: diva2:1154877
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QC 20171106

Tilgjengelig fra: 2017-11-06 Laget: 2017-11-06 Sist oppdatert: 2017-11-06bibliografisk kontrollert

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Blom, HansNilsson, LinneaBrismar, Hjalmar

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