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Tunable Ampere phase plate for low dose imaging of biomolecular complexes
Forschungszentrum Julich, Ernst Ruska Ctr Microscopy & Spect Electrons, D-52428 Julich, Germany.;Forschungszentrum Julich, Peter Grunberg Inst, D-52428 Julich, Germany..
Tech Univ Denmark, Ctr Elect Nanoscopy, DK-2800 Lyngby, Denmark..
Forschungszentrum Julich, Ernst Ruska Ctr Microscopy & Spect Electrons, D-52428 Julich, Germany.;Forschungszentrum Julich, Peter Grunberg Inst, D-52428 Julich, Germany..
FEI Co, Achtseweg Noord 5, NL-5600 KA Eindhoven, Netherlands..
Vise andre og tillknytning
2018 (engelsk)Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 8, artikkel-id 5592Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.

sted, utgiver, år, opplag, sider
NATURE PUBLISHING GROUP , 2018. Vol. 8, artikkel-id 5592
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Identifikatorer
URN: urn:nbn:se:kth:diva-228130DOI: 10.1038/s41598-018-23100-3ISI: 000429095600001PubMedID: 29618785Scopus ID: 2-s2.0-85044965282OAI: oai:DiVA.org:kth-228130DiVA, id: diva2:1207121
Merknad

QC 20180518

Tilgjengelig fra: 2018-05-18 Laget: 2018-05-18 Sist oppdatert: 2018-05-18bibliografisk kontrollert

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