Single-particle imaging without symmetry constraints at an X-ray free-electron laserDESY, Notkestr 85, D-22607 Hamburg, Germany..
DESY, Notkestr 85, D-22607 Hamburg, Germany..
Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany..
SLAC Natl Accelerator Lab, Linac Coherent Light Source, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA..
Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Uppsala, Sweden..
European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany.;Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Uppsala, Sweden..
Brookhaven Natl Lab, 98 Rochester St, Shirley, NY 11967 USA..
Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Uppsala, Sweden.;Lawrence Berkeley Natl Lab, NERSC, Berkeley, CA USA..
Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany..
Natl Res Ctr, Kurchatov Inst, Akad Kurchatova Pl 1, Moscow 123182, Russia..
European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany..
Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany..
Arizona State Univ, Biodesign Inst, Biodesign Ctr Immunotherapy Vaccines & Virotherap, Tempe, AZ 85287 USA.;Arizona State Univ, Biodesign Inst, Biodesign Ctr Appl Struct Discovery, Tempe, AZ 85287 USA.;Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA..
SLAC Natl Accelerator Lab, Linac Coherent Light Source, 2575 Sand Hill Rd, Menlo Pk, CA 94025 USA..
Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany..
DESY, Notkestr 85, D-22607 Hamburg, Germany.;Natl Res Nucl Univ, MEPhI Moscow Engn Phys Inst, Kashirskoe Shosse 31, Moscow 115409, Russia..
Vise andre og tillknytning
2018 (engelsk)Inngår i: IUCrJ, E-ISSN 2052-2525, Vol. 5, s. 727-736Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]
The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single-particle data is developed. It consists of several well defined steps including single-hit diffraction data classification, refined filtering of the classified data, reconstruction of three-dimensional scattered intensity from the experimental diffraction patterns by orientation determination and a final three-dimensional reconstruction of the virus electron density without symmetry constraints. The analysis developed here revealed and quantified nanoscale features of the PR772 virus measured in this experiment, with the obtained resolution better than 10 nm, with a clear indication that the structure was compressed in one direction and, as such, deviates from ideal icosahedral symmetry.
sted, utgiver, år, opplag, sider
INT UNION CRYSTALLOGRAPHY , 2018. Vol. 5, s. 727-736
Emneord [en]
single-particle imaging, three-dimensional virus reconstructions, XFELs
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-239079DOI: 10.1107/S205225251801120XISI: 000448982300010PubMedID: 30443357Scopus ID: 2-s2.0-85056165262OAI: oai:DiVA.org:kth-239079DiVA, id: diva2:1264915
Forskningsfinansiär
Swedish Research CouncilSwedish Foundation for Strategic Research
Merknad
QC 20181121
2018-11-212018-11-212022-09-28bibliografisk kontrollert