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Spontaneous calcium activity in metanephric mesenchymal cells regulates branching morphogenesis in the embryonic kidney
KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
KTH, Centra, Science for Life Laboratory, SciLifeLab. KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik.ORCID-id: 0000-0002-5584-9170
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2019 (engelsk)Inngår i: The FASEB Journal, ISSN 0892-6638, E-ISSN 1530-6860, Vol. 33, nr 3, s. 4089-4096Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The central role of calcium signaling during development of early vertebrates is well documented, but little is known about its role in mammalian embryogenesis. We have used immunofluorescence and time-lapse calcium imaging of cultured explanted embryonic rat kidneys to study the role of calcium signaling for branching morphogenesis. In mesenchymal cells, we recorded spontaneous calcium activity that was characterized by irregular calcium transients. The calcium signals were dependent on release of calcium from intracellular stores in the endoplasmic reticulum. Down-regulation of the calcium activity, both by blocking the sarco-endoplasmic reticulum Ca2+-ATPase and by chelating cytosolic calcium, resulted in retardation of branching morphogenesis and a reduced formation of primitive nephrons but had no effect on cell proliferation. We propose that spontaneous calcium activity contributes with a stochastic factor to the self-organizing process that controls branching morphogenesis, a major determinant of the ultimate number of nephrons in the kidney.Fontana, J. M., Khodus, G. R., Unnersjo-Jess, D., Blom, H., Aperia, A., Brismar, H. Spontaneous calcium activity in metanephric mesenchymal cells regulates branching morphogenesis in the embryonic kidney.

sted, utgiver, år, opplag, sider
FEDERATION AMER SOC EXP BIOL , 2019. Vol. 33, nr 3, s. 4089-4096
Emneord [en]
organogenesis, nephron, calcium imaging
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-246250DOI: 10.1096/fj.201802054RISI: 000459794800079PubMedID: 30496703OAI: oai:DiVA.org:kth-246250DiVA, id: diva2:1301596
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QC 20190402

Tilgjengelig fra: 2019-04-02 Laget: 2019-04-02 Sist oppdatert: 2019-04-02bibliografisk kontrollert

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Fontana, Jacopo M.Unnersjö Jess, DavidBlom, HansBrismar, Hjalmar

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Fontana, Jacopo M.Khodus, Georgiy R.Unnersjö Jess, DavidBlom, HansBrismar, Hjalmar
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