Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Ankyrin B Modulates the Function of Na,K-ATPase/Inositol 1,4,5-Trisphosphate Receptor Signaling Microdomain
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.
Vise andre og tillknytning
2008 (engelsk)Inngår i: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 283, nr 17, s. 11461-11468Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Na, K-ATPase and inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) can form a signaling microdomain that in the presence of ouabain triggers highly regular calcium oscillations. Downstream effects include NF-kappa B activation. Here we report that ankyrin B (Ank-B), expressed in most mammalian cells, plays a pivotal role in the function of the Na, K-ATPase/ IP3R signaling microdomain. In studies performed on a monkey kidney cell line, we show that Ank-B co-precipitates with both Na, K-ATPase and IP3R. We identify the N terminus tail of the Na, K-ATPase catalytic subunit and the N-terminal portion 1-604 of the IP3R as novel binding sites for Ank-B. Knockdown of Ank-B with small interfering RNA reduced the expression of Ank-B to 15-30%. This down-regulation of Ank-B attenuated the interaction between Na, K-ATPase and IP3R, reduced the number of cells responding to pM doses of ouabain with calcium oscillations, altered the calcium oscillatory pattern, and abolished the ouabain effect on NF-kappa B. In contrast, Ank-B down-regulation had no effect on the ion transporting function of Na, K-ATPase and no effect on the distribution and apparent mobility of Na, K-ATPase in the plasma membrane.

sted, utgiver, år, opplag, sider
2008. Vol. 283, nr 17, s. 11461-11468
Emneord [en]
generates calcium oscillations, nf-kappa-b, cardiac-arrhythmia, catalytic subunit, serum deprivation, binding domain, na+/k+-atpase, ip3 receptor, na, k-atpase, identification
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-11817DOI: 10.1074/jbc.M706942200ISI: 000255067400044PubMedID: 18303017Scopus ID: 2-s2.0-45549103740OAI: oai:DiVA.org:kth-11817DiVA, id: diva2:283474
Merknad
QC 20100806Tilgjengelig fra: 2009-12-28 Laget: 2009-12-28 Sist oppdatert: 2022-06-25bibliografisk kontrollert
Inngår i avhandling
1. Studies of Cellular Responses to External Stimuli in Engineered Microenvironments
Åpne denne publikasjonen i ny fane eller vindu >>Studies of Cellular Responses to External Stimuli in Engineered Microenvironments
2009 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
sted, utgiver, år, opplag, sider
Stockholm: KTH, 2009. s. xvi, 59
Serie
Trita-FYS, ISSN 0280-316X ; 09:73
HSV kategori
Identifikatorer
urn:nbn:se:kth:diva-11819 (URN)978-91-7415-529-7 (ISBN)
Disputas
2010-01-15, Sal FD5, AlbaNova, Roslagstullsbacken 21, Stockholm, 13:00 (engelsk)
Opponent
Veileder
Merknad
QC 20100806Tilgjengelig fra: 2009-12-29 Laget: 2009-12-29 Sist oppdatert: 2022-06-25bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Person

Brismar, Hjalmar

Søk i DiVA

Av forfatter/redaktør
Rydholm, SusannaBrismar, HjalmarAperia, Anita
Av organisasjonen
I samme tidsskrift
Journal of Biological Chemistry

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 110 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf