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Phospholipase C zeta, the trigger of egg activation in mammals, is present in a non-mammalian species
Vise andre og tillknytning
2005 (engelsk)Inngår i: Reproduction, ISSN 1470-1626, E-ISSN 1476-3990, Vol. 130, nr 2, s. 157-163Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The activation of the egg to begin development into an embryo is triggered by a sperm-induced increase in intracellular egg Ca2+. There has been much controversy about how the sperm induces this fundamental developmental event, but recent studies suggest that, in mammals, egg activation is triggered by a testis-specific phospholipase C: PLC zeta. Since the discovery of PLC zeta, it has been unclear whether its role in triggering egg activation is common to all vertebrates, or is confined to mammals. Here, we demonstrate for the first time that PLC zeta is present in a non-mammalian vertebrate. Using genomic and cDNA databases, we have identified the cDNA encoding a PLC zeta orthologue in the domestic chicken that, like the mammalian isoforms, is a testis-specific gene. The chicken PLC zeta cDNA is 2152 bp in size and encodes an open reading frame of 639 amino acids. When injected into mouse oocytes, chicken PLC zeta cRNA triggers Ca2+ oscillations, indicating that it has functional properties similar to those of mammalian PLC zeta. Our findings suggest that PLC zeta may have a universal role in triggering egg activation in vertebrates.

sted, utgiver, år, opplag, sider
2005. Vol. 130, nr 2, s. 157-163
Emneord [en]
ca2+ oscillations, mouse oocytes, calcium oscillations, protein factor, plc-zeta, fertilization, sequence, sensitivity, maturation, contain
Identifikatorer
URN: urn:nbn:se:kth:diva-14995DOI: 10.1530/rep.1.00707ISI: 000231460900003PubMedID: 16049153Scopus ID: 2-s2.0-23844457941OAI: oai:DiVA.org:kth-14995DiVA, id: diva2:333036
Merknad
QC 20100525Tilgjengelig fra: 2010-08-05 Laget: 2010-08-05 Sist oppdatert: 2022-06-25bibliografisk kontrollert

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Savolainen, Peter

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