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Photoderivatized polymer thin films at quartz crystal microbalance surfaces: Sensors for carbohydrate-protein interactions
KTH, Skolan för kemivetenskap (CHE), Kemi, Organisk kemi.
KTH, Skolan för kemivetenskap (CHE), Kemi, Organisk kemi.
KTH, Skolan för kemivetenskap (CHE), Kemi, Organisk kemi.
KTH, Skolan för kemivetenskap (CHE), Kemi, Organisk kemi.
Vise andre og tillknytning
2007 (engelsk)Inngår i: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 79, nr 18, s. 6897-6902Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Photoderivatized polymer-coated gold surfaces have been developed following a perfluorophenylazide-based double ligation strategy. Gold-plated quartz crystal microbalance (QCM) crystals were initially covalently functionalized with a monolayer of poly(ethylene glycol) (PEG), using photo-or thermolytic nitrene formation and insertion. The polymer surfaces were subsequently used as substrates for photoinsertion of carbohydrate-derivatized photoprobes, yielding different recognition motifs for selective protein binding. The resulting robust and biocompatible sensor surfaces were applied to a flow-through QCM instrument for monitoring lectin-carbohydrate interactions in real time. The results clearly show the predicted lectin selectivity, demonstrating the applicability of the approach.

sted, utgiver, år, opplag, sider
2007. Vol. 79, nr 18, s. 6897-6902
Emneord [en]
functionalized perfluorophenyl azides, ligand-binding characteristics, lectin concanavalin-a, monomer/dimer equilibrium, glycolipid monolayers, plasmon resonance, mistletoe lectin, pisum-sativum, affinity, mannan
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Identifikatorer
URN: urn:nbn:se:kth:diva-16958DOI: 10.1021/ac070740rISI: 000249527700001PubMedID: 17705448Scopus ID: 2-s2.0-34548783269OAI: oai:DiVA.org:kth-16958DiVA, id: diva2:335001
Merknad
QC 20100525Tilgjengelig fra: 2010-08-05 Laget: 2010-08-05 Sist oppdatert: 2020-03-09bibliografisk kontrollert

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Pei, YuxinYu, HuiPei, ZhichaoTheurer, MatthiasAmmer, CarolinYan, MingdiRamström, Olof
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