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Micromachined filter-chamber array with passive valves for biochemical assays on beads
KTH, Tidigare Institutioner (före 2005), Bioteknologi.
KTH, Tidigare Institutioner (före 2005), Signaler, sensorer och system.ORCID-id: 0000-0001-8248-6670
KTH, Tidigare Institutioner (före 2005), Signaler, sensorer och system.ORCID-id: 0000-0001-9552-4234
2001 (engelsk)Inngår i: Electrophoresis, ISSN 0173-0835, E-ISSN 1522-2683, Vol. 22, nr 2, s. 249-257Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The filter-chamber array presented here enables a real-time parallel analysis of three different samples on beads in a volume of 3 nL, on a 1 cm(2) chip. The filter-chamber array is a system containing three filter-chambers, three passive valves at the inlet channels and a common outlet. The design enables parallel sample handling and time-controlled analysis. The device is microfabricated in silicon and sealed with a Pyrex lid to enable real-time analysis. Single nucleotide polymorphism analysis by using pyrose-quencing has successfully been performed in single filter-chamber devices. The passive valves consist of plasma-deposited octafluorocyclobutane and show a much higher resistance towards water and surface-active solutions than previous hydrophobic patches. The device is not sensitive to gas bubbles, clogging is rare and reversible, and the filter-chamber array is reusable. More complex (bio)chemical reactions on beads can be performed in the devices with passive valves than in the devices without valves.

sted, utgiver, år, opplag, sider
2001. Vol. 22, nr 2, s. 249-257
Emneord [en]
bead trapping, microfluidic array, hydrophobic valve, pyrosequencing, single nucleotide polymorphism
Identifikatorer
URN: urn:nbn:se:kth:diva-20335DOI: 10.1002/1522-2683(200101)22:2<249::AID-ELPS249>3.0.CO;2-4ISI: 000166783500009PubMedID: 11288892Scopus ID: 2-s2.0-0035142937OAI: oai:DiVA.org:kth-20335DiVA, id: diva2:339030
Merknad
QC 20100525Tilgjengelig fra: 2010-08-10 Laget: 2010-08-10 Sist oppdatert: 2022-06-25bibliografisk kontrollert

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van der Wijngaart, WouterStemme, Göran

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