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Measurement of interactions between protein layers adsorbed on silica by atomic force microscopy
Vise andre og tillknytning
2004 (engelsk)Inngår i: Journal of Physics: Condensed Matter, ISSN 0953-8984, E-ISSN 1361-648X, Vol. 16, nr 26, s. S2383-S2392Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The present work, using an atomic force microscope and the colloid probe technique, investigates the interaction forces between bovine serum albumin (BSA) layers and between apoferritin layers adsorbed on silica surfaces. The measurements have been carried out in an aqueous medium at different pH values and NaCl concentrations. Similar behaviours have been found with both proteins. Electrostatic and steric forces dominate the interactions between the protein layers at low NaCl concentrations. However, a very strange behaviour is found as a function of pH at high NaCl concentrations. The results obtained under these conditions could be explained if the presence of hydration forces in these systems is assumed.

sted, utgiver, år, opplag, sider
2004. Vol. 16, nr 26, s. S2383-S2392
Emneord [en]
bovine serum-albumin, colloidal stability, hydration forces, apoferritin, scattering, adsorption, lysozyme, aggregation, membranes, cataract
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-23625DOI: 10.1088/0953-8984/16/26/011ISI: 000223086300013Scopus ID: 2-s2.0-3142721401OAI: oai:DiVA.org:kth-23625DiVA, id: diva2:342324
Merknad
QC 20100525 QC 20110926. Meeting on Biological Surfaces and Interfaces. Castelvecchio Pascoli, ITALY. JUN, 2003 Tilgjengelig fra: 2010-08-10 Laget: 2010-08-10 Sist oppdatert: 2017-12-12bibliografisk kontrollert

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Feiler, AdamRutland, Mark W
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