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Cultivation of COS7-cells using extracellular matrix in 3D microfluidic surface enlarged structure
KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.ORCID-id: 0000-0002-3996-9279
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.
KTH, Skolan för elektro- och systemteknik (EES), Mikrosystemteknik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Cellens fysik.ORCID-id: 0000-0003-0578-4003
Vise andre og tillknytning
2005 (engelsk)Inngår i: Micro Total Analysis Systems 2004, 2005, nr 297, s. 261-263Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

This paper presents a novel method to cultivate cells in a controlled 3D surface enlarged micro-environment. The 3D environment is achieved by insertion of a gel-like extracellular matrix (ECM) mixed with cells into a micromachined silicon fluid structure. Shrinking of the gel enables further flow through the channel. Due to the structure design the shrinking is non-uniform, which results in an increased surface area. With the proposed design cells are alive and viable after 72 h of incubation within the chip.

sted, utgiver, år, opplag, sider
2005. nr 297, s. 261-263
Serie
ROYAL SOCIETY OF CHEMISTRY SPECIAL PUBLICATIONS, ISSN 0260-6291
Emneord [en]
3D-cultivation, matrigel, ECM, microfluidic structure
Identifikatorer
URN: urn:nbn:se:kth:diva-24781ISI: 000229017400086ISBN: 0-85404-896-0 (tryckt)OAI: oai:DiVA.org:kth-24781DiVA, id: diva2:355272
Konferanse
8th International Conference on Miniaturized Systems for Chemistry and Life Sciences Malmo, SWEDEN, SEP 26-30, 2004
Merknad
QC 20101006Tilgjengelig fra: 2010-10-06 Laget: 2010-09-27 Sist oppdatert: 2010-10-06bibliografisk kontrollert

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