Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Development of a fed-batch process for the production of a recombinant protein X in CHO-GS system: Case study from the cell to reactor process ready for pilot scale cultivation
KTH, Skolan för bioteknologi (BIO), Bioprocessteknik. (Cell Technology Group)ORCID-id: 0000-0002-5370-4621
Biovitrum/SOBI, Sweden.
Biovitrum/SOBI, Sweden.
Biovitrum/SOBI, Sweden.
Vise andre og tillknytning
2010 (engelsk)Inngår i: Cells and Culture: Proceedings of the 20th ESACT / [ed] Noll T, Springer Science+Business Media B.V., 2010, s. 723-725Konferansepaper, Publicerat paper (Annet vitenskapelig)
Abstract [en]

A new cell line was created using CHO-GS system. The most promising clones were adapted to different base cultivation media leading to the selection of one medium. The fed-batch process development was performed in spinner, shake flask and bioreactor scale. It included the selection of a feed medium, the choice of the feed strategy and the optimisation of the glucose feeding. The process was then simplified by using a single feed including the feed medium and the glucose feed. Finally up-scaling parameters like aeration and CO2 stripping were studied in 3 L and 15 L bioreactors in preparation for pilot scale operation. This process proved to be robust, reproducible and suitable for large and commercial scale operation.

sted, utgiver, år, opplag, sider
Springer Science+Business Media B.V., 2010. s. 723-725
Emneord [en]
CHO·GS·Fed-batch·Feed·Up-scale·Bioreactors
HSV kategori
Forskningsprogram
SRA - Molekylär biovetenskap
Identifikatorer
URN: urn:nbn:se:kth:diva-86350DOI: 10.1007/978-90-481-3419-9_125ISBN: 978-90-481-3418-2 (tryckt)OAI: oai:DiVA.org:kth-86350DiVA, id: diva2:500653
Konferanse
Proceedings of the 20th ESACT, Dresden, Germany, June 17-20, 2007
Merknad
QC 20120420Tilgjengelig fra: 2012-02-13 Laget: 2012-02-13 Sist oppdatert: 2012-04-20bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekst

Personposter BETA

Chotteau, Veronique

Søk i DiVA

Av forfatter/redaktør
Chotteau, Veronique
Av organisasjonen

Søk utenfor DiVA

GoogleGoogle Scholar

doi
isbn
urn-nbn

Altmetric

doi
isbn
urn-nbn
Totalt: 665 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf