Åpne denne publikasjonen i ny fane eller vindu >>2017 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]
The search for alternatives to oil-based monomers has sparked interest for scientists to focus on the use of renewable resources for energy production, for the synthesis of polymeric materials and in other areas. With the use of renewable resources, scientists face new challenges to first isolate interesting molecules and then to process them.
Enzymes are nature’s own powerful catalysts and display a variety of activities. They regulate important functions in life. They can also be used for chemical synthesis due to their efficiency, selectivity and mild reaction conditions. The selectivity of the enzyme allows specific reactions enabling the design of building blocks for polymers.
In the work presented here, a lipase (Candida antarctica lipase B (CalB)) was used to produce building blocks for polymers. An efficient route was developed to selectively process epoxy-functional fatty acids into resins with a variety of functional groups (maleimide, oxetane, thiol, methacrylate). These oligoester structures, based on epoxy fatty acids from birch bark and vegetable oils, could be selectively cured to form thermosets with tailored properties.
The specificity of an esterase with acyl transfer activity from Mycobacterium smegmatis (MsAcT) was altered by rational design. The produced variants increased the substrate scope and were then used to synthesize amides in water, where the wild type showed no conversion. A synthetic procedure was developed to form mixed dicarboxylic esters by selectively reacting only one side of divinyl adipate in order to introduce additional functional groups.
sted, utgiver, år, opplag, sider
Stockholm: KTH Royal Institute of Technology, 2017. s. 61
Serie
TRITA-BIO-Report, ISSN 1654-2312 ; 2017:15
Emneord
Enzyme, Enzyme Engineering, Biocatalysis, Lipase, CalB, MsAcT, Substrate specificity, Selectivity, Polymer Chemistry, Polymer Synthesis
HSV kategori
Forskningsprogram
Bioteknologi
Identifikatorer
urn:nbn:se:kth:diva-212499 (URN)978-91-7729-494-8 (ISBN)
Disputas
2017-09-22, Kollegiesalen, Brinellvägen 8, Stockholm, 10:00
Opponent
Veileder
Merknad
QC 20170823
2017-08-232017-08-222022-06-27bibliografisk kontrollert