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High density custom microarrays formed by microcompartment amplification on glass surface
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi.
Vise andre og tillknytning
2014 (engelsk)Inngår i: 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society , 2014, s. 1027-1029Konferansepaper, Publicerat paper (Fagfellevurdert)
Abstract [en]

Compartmentalization of a single DNA molecule is necessary for digital amplification. In the present study we have developed a microscale isothermal amplification using exponential rolling circle amplification (RCA). RCA was performed in PDMS microcompartments on a microarray glass, with a volume of less than 1 pL. Resulting amplicons were attached to the glass surface and formed a custom array with the density of spots above 2,5 × 105 per cm2. Our technology can be applied for digital amplification of DNA or RNA from a variety of complex biological samples in a microchip format.

sted, utgiver, år, opplag, sider
Chemical and Biological Microsystems Society , 2014. s. 1027-1029
Emneord [en]
Digital, Microcompartments, RCA, Solid surface amplification, Bioassay, Microarrays, Nucleic acids, Biological samples, Isothermal amplifications, Micro-compartments, Rolling circle amplifications, Single DNA molecules, Solid surface, Glass
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-174808Scopus ID: 2-s2.0-84941662700ISBN: 9780979806476 (tryckt)OAI: oai:DiVA.org:kth-174808DiVA, id: diva2:881628
Konferanse
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, 26 October 2014 through 30 October 2014
Merknad

QC 20151211

Tilgjengelig fra: 2015-12-11 Laget: 2015-10-07 Sist oppdatert: 2018-01-10bibliografisk kontrollert

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Totalt: 44 treff
RefereraExporteraLink to record
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  • apa
  • harvard1
  • ieee
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  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
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Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf