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Polymorphism of curdlan and (1 -> 3)-beta-D-glucans synthesized in vitro: A C-13 CP-MAS and X-ray diffraction analysis
KTH, Skolan för bioteknologi (BIO), Glykovetenskap.
2006 (Engelska)Ingår i: Carbohydrate Polymers, ISSN 0144-8617, E-ISSN 1879-1344, Vol. 66, nr 2, s. 199-207Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The polymorphism of three different forms of curdlan and four (1 -> 3)-beta-D-glucans synthesized in vitro was investigated by C-13 cross-polarization/magic angle spinning nuclear magnetic resonance (CP/MAS NMR) spectroscopy and X-ray powder diffraction. Dried samples of curdlan presented a disordered state whereas two distinct hydrated crystalline structures were evidenced for the polymer after hydrothermal treatment or swelling in water at room temperature. The samples synthesized in vitro by detergent extracts of plasma membranes from Rubus fruticosus and Saprolegnia monoica and by a mutated barley (1 -> 3)-beta-D-glucan endohydrolase exhibited a structural heterogeneity that can be explained in the light of the results obtained on standard samples. A 76-ppm resonance signal corresponding to carbon five was identified by CP/MAS NMR spectroscopy. The relative importance of this peak was shown to be linked to the proportion of the different crystalline allomorphs in a given sample. This peak can be considered as a new marker of the degree of organization of (1 -> 3)-beta-D-glucans. The observed polymorphism provides further detailed information on the conformation of the different (1 -> 3)P-D-glucan allomorphs.

Ort, förlag, år, upplaga, sidor
2006. Vol. 66, nr 2, s. 199-207
Nyckelord [en]
(1 -> 3)-beta-d-glucans, curdlan, triple helix, single helix, biopolymers, biosynthesis of polysaccharides, CP/MAS NMR spectroscopy, X-ray diffraction, triple-helix conformation, coagulation factor-g, solid-state, biological response, antitumor-activity, packing analysis, resilient gel, (1->3)-beta-d-glucans, nmr, activation
Identifikatorer
URN: urn:nbn:se:kth:diva-16082ISI: 000241489900007OAI: oai:DiVA.org:kth-16082DiVA, id: diva2:334124
Anmärkning
QC 20100525Tillgänglig från: 2010-08-05 Skapad: 2010-08-05 Senast uppdaterad: 2017-12-12Bibliografiskt granskad

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